Reviewed, UniProtKB/Swiss-Prot Q8W420 (ADO2_ARATH)

Names and origin

Protein names

Recommended name:
    Adagio protein 2
Alternative name(s):
    LOV kelch protein 2
    Flavin-binding kelch repeat F-box protein 1-like protein 1
      Short name=FKF1-like protein 1
    F-box only protein 2c
      Short name=FBX2c

Gene names

Name:	ADO2
Synonyms:	FKL1, LKP2
Ordered Locus Names:	At2g18915
ORF Names:	F19F24.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	611 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of E3 ubiquitin ligase complexes that plays a central role blue-light-dependent circadian cycles. Acts as a blue-light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO2) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including transition to flowering time, hypocotyl elongation, cotyledons and leaves movement rhythms. APRR1 and APRR5 seem to be substrates of the SCF(ADO1) complex. Ref.2
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Self-associates. Interacts with ADO1, AD03, SKP1A/ASK1, SKP1B/ASK2, SKP1C/ASK3, SKP1D/ASK4, SKP1E/ASK5, SKP1K/ASK11, SKP1N/ASK14, SKP1TA/ASK20A, SKP1TB/ASK20B, APRR1, APRR5, DI19 and COL1. Ref.6 Ref.7
Subcellular location	Nucleus. Ref.6 Ref.7
Tissue specificity	Weakly expressed in seedlings, root tips, stems, leaves, flowers, young siliques, sepals and seeds. Ref.2 Ref.6
Post-translational modification	FMN binds covalently to cysteine after exposure to blue light and is reversed in the dark By similarity.
Miscellaneous	'Adagio' means slowly in Italian.
Sequence similarities	Belongs to the ADAGIO family. Contains 1 F-box domain. Contains 5 Kelch repeats. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
Biological process	Biological rhythms Sensory transduction Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Kelch repeat Repeat
Ligand	Chromophore FMN Flavoprotein
Molecular function	Photoreceptor protein Receptor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW regulation of circadian rhythm Ref.2 Inferred from mutant phenotype. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW rhythmic process Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	Cajal body Ref.7 Inferred from direct assay. Source: TAIR cytosol Ref.7 Inferred from direct assay. Source: TAIR
Molecular function	photoreceptor activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.7 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APRR1	Q9LKL2	2	EBI-1015688,EBI-618423
DOF3.3	Q8LFV3	3	EBI-1015688,EBI-1536119
DOF5.2	Q93ZL5	3	EBI-1015688,EBI-1536103
DOF5.5	Q8W1E3	1	EBI-1015688,EBI-1536051
GI	Q9SQI2	2	EBI-1015688,EBI-446380

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8W420-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8W420-2)

The sequence of this isoform differs from the canonical sequence as follows:
     79-88: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 611	611	Adagio protein 2		PRO_0000119957
Regions
	Domain	35 – 114	80	PAS
	Domain	118 – 158	41	PAC
	Domain	196 – 242	47	F-box
	Repeat	293 – 343	51	Kelch 1
	Repeat	358 – 396	39	Kelch 2
	Repeat	397 – 446	50	Kelch 3
	Repeat	463 – 515	53	Kelch 4
	Repeat	527 – 579	53	Kelch 5
Amino acid modifications
	Modified residue	82	1	S-4a-FMN cysteine By similarity
Natural variations
	Alternative sequence	79 – 88	10	Missing in isoform 2.		VSP_016048

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: FDB9F994CBF0D017	FASTA	611	66,351
	10 20 30 40 50 60 MQNQMEWDSD SDLSGGDEVA EDGWFGGDNG AIPFPVGSLP GTAPCGFVVS DALEPDNPII 70 80 90 100 110 120 YVNTVFEIVT GYRAEEVIGR NCRFLQCRGP FTKRRHPMVD STIVAKMRQC LENGIEFQGE 130 140 150 160 170 180 LLNFRKDGSP LMNKLRLVPI REEDEITHFI GVLLFTDAKI DLGPSPDLSA KEIPRISRSF 190 200 210 220 230 240 TSALPIGERN VSRGLCGIFE LSDEVIAIKI LSQLTPGDIA SVGCVCRRLN ELTKNDDVWR 250 260 270 280 290 300 MVCQNTWGTE ATRVLESVPG AKRIGWVRLA REFTTHEATA WRKFSVGGTV EPSRCNFSAC 310 320 330 340 350 360 AVGNRIVIFG GEGVNMQPMN DTFVLDLGSS SPEWKSVLVS SPPPGRWGHT LSCVNGSRLV 370 380 390 400 410 420 VFGGYGSHGL LNDVFLLDLD ADPPSWREVS GLAPPIPRSW HSSCTLDGTK LIVSGGCADS 430 440 450 460 470 480 GALLSDTFLL DLSMDIPAWR EIPVPWTPPS RLGHTLTVYG DRKILMFGGL AKNGTLRFRS 490 500 510 520 530 540 NDVYTMDLSE DEPSWRPVIG YGSSLPGGMA APPPRLDHVA ISLPGGRILI FGGSVAGLDS 550 560 570 580 590 600 ASQLYLLDPN EEKPAWRILN VQGGPPRFAW GHTTCVVGGT RLVVLGGQTG EEWMLNEAHE 610 LLLATSTTAS T
	Isoform 2. Checksum: 2860CE717F0CBD5F	FASTA	601	65,116
	10 20 30 40 50 60 MQNQMEWDSD SDLSGGDEVA EDGWFGGDNG AIPFPVGSLP GTAPCGFVVS DALEPDNPII 70 80 90 100 110 120 YVNTVFEIVT GYRAEEVIGP FTKRRHPMVD STIVAKMRQC LENGIEFQGE LLNFRKDGSP 130 140 150 160 170 180 LMNKLRLVPI REEDEITHFI GVLLFTDAKI DLGPSPDLSA KEIPRISRSF TSALPIGERN 190 200 210 220 230 240 VSRGLCGIFE LSDEVIAIKI LSQLTPGDIA SVGCVCRRLN ELTKNDDVWR MVCQNTWGTE 250 260 270 280 290 300 ATRVLESVPG AKRIGWVRLA REFTTHEATA WRKFSVGGTV EPSRCNFSAC AVGNRIVIFG 310 320 330 340 350 360 GEGVNMQPMN DTFVLDLGSS SPEWKSVLVS SPPPGRWGHT LSCVNGSRLV VFGGYGSHGL 370 380 390 400 410 420 LNDVFLLDLD ADPPSWREVS GLAPPIPRSW HSSCTLDGTK LIVSGGCADS GALLSDTFLL 430 440 450 460 470 480 DLSMDIPAWR EIPVPWTPPS RLGHTLTVYG DRKILMFGGL AKNGTLRFRS NDVYTMDLSE 490 500 510 520 530 540 DEPSWRPVIG YGSSLPGGMA APPPRLDHVA ISLPGGRILI FGGSVAGLDS ASQLYLLDPN 550 560 570 580 590 600 EEKPAWRILN VQGGPPRFAW GHTTCVVGGT RLVVLGGQTG EEWMLNEAHE LLLATSTTAS T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An Arabidopsis circadian clock component interacts with both CRY1 and phyB." Jarillo J.A., Capel J., Tang R.-H., Yang H.-Q., Alonso J.M., Ecker J.R., Cashmore A.R. Nature 410:487-490(2001) [PubMed: 11260718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"A role for LKP2 in the circadian clock of Arabidopsis." Schultz T.F., Kiyosue T., Yanovsky M., Wada M., Kay S.A. Plant Cell 13:2659-2670(2001) [PubMed: 11752379] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INDUCTION.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"F-box proteins in Arabidopsis." Xiao W., Jang J.-C. Trends Plant Sci. 5:454-457(2000) [PubMed: 11077244] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis." Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T. J. Exp. Bot. 55:2015-2027(2004) [PubMed: 15310821] [Abstract] Cited for: INTERACTION WITH SKP1A; SKP1B; SKP1C; SKP1D; SKP1E; SKP1K; SKP1N; SKP1TA; SKP1TB; ADO1; AD03; APRR1 AND APRR5, SELF-ASSOCIATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[7]	"Identification of LOV KELCH PROTEIN2 (LKP2)-interacting factors that can recruit LKP2 to nuclear bodies." Fukamatsu Y., Mitsui S., Yasuhara M., Tokioka Y., Ihara N., Fujita S., Kiyosue T. Plant Cell Physiol. 46:1340-1349(2005) [PubMed: 15937324] [Abstract] Cited for: INTERACTION WITH DI19 AND COL1, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF252295 mRNA. Translation: AAK27434.1. Different initiation. AB038797 mRNA. Translation: BAB83169.1. AC003673 Genomic DNA. Translation: AAM14891.1. Different initiation. AY099709 mRNA. Translation: AAM20560.1.
IPI	IPI00524842. IPI00528666.
RefSeq	NP_565444.2. NP_849983.1.
UniGene	At.19440
3D structure databases
HSSP	HSSP built from PDB template 1N9L based on UniProtKB Q8LPE0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8W420. 17 interactions.
Proteomic databases
PRIDE	Q8W420.
Genome annotation databases
GeneID	816408.
GenomeReviews	Gene locus AT2G18915 in contig CT485783_GR.
KEGG	ath:AT2G18915.
NMPDR	fig|3702.1.peg.8887.
Organism-specific databases
GeneFarm	5107. 485.
TAIR	At2g18915.
Gene expression databases
GermOnline	AT2G18915. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001810. F-box. IPR015915. Kelch-typ_b-propeller. IPR006652. Kelch_1. IPR011498. Kelch_2. IPR001610. PAC. IPR000014. PAS. [Graphical view]
Gene3D	G3DSA:2.120.10.80. Kelch-typ_b-propeller. 1 hit.
Pfam	PF00646. F-box. 1 hit. PF01344. Kelch_1. 1 hit. PF07646. Kelch_2. 3 hits. [Graphical view]
SMART	SM00086. PAC. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. False negative. PS50113. PAC. False negative. PS50112. PAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADO2_ARATH

Accession

Primary (citable) accession number: Q8W420
Secondary accession number(s): Q9C5S6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	March 1, 2002
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)