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Protein

Adagio protein 2

Gene

ADO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of an E3 ubiquitin ligase complex that plays a central role in blue light-dependent circadian cycles. Acts as a blue light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO2) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including the transition to flowering time, hypocotyl elongation, cotyledons and leaf movement rhythms. APRR1/TOC1 and APRR5 seem to be substrates of the SCF(ADO2) complex. ADO2 interacts with ADO3 and export it to cytoplasmic speckles, preventing the interaction of ADO3 with CDF1. Ubiquitination of ADO2 is not involved in this recruitment.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • flower development Source: UniProtKB-KW
  • protein-chromophore linkage Source: UniProtKB-KW
  • protein ubiquitination Source: UniProtKB-UniPathway
  • regulation of circadian rhythm Source: TAIR
  • rhythmic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Photoreceptor protein, Receptor

Keywords - Biological processi

Biological rhythms, Flowering, Sensory transduction, Ubl conjugation pathway

Keywords - Ligandi

Chromophore, Flavoprotein, FMN

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Adagio protein 2
Alternative name(s):
F-box only protein 2c
Short name:
FBX2c
Flavin-binding kelch repeat F-box protein 1-like protein 1
Short name:
FKF1-like protein 1
LOV kelch protein 2
Gene namesi
Name:ADO2
Synonyms:FKL1, LKP2
Ordered Locus Names:At2g18915
ORF Names:F19F24.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G18915.

Subcellular locationi

GO - Cellular componenti

  • Cajal body Source: TAIR
  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Accumulation of ADO3 protein during the morning period but no effect on flowering time.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi283 – 2831K → R: No effect on the recruitment of ADO3 to the cytoplasm; when associated with R-335; R-410; R-463; R-472 and R-553. 1 Publication
Mutagenesisi335 – 3351K → R: No effect on the recruitment of ADO3 to the cytoplasm; when associated with R-283; R-410; R-463; R-472 and R-553. 1 Publication
Mutagenesisi410 – 4101K → R: No effect on the recruitment of ADO3 to the cytoplasm; when associated with R-283; R-335; R-463; R-472 and R-553. 1 Publication
Mutagenesisi463 – 4631K → R: No effect on the recruitment of ADO3 to the cytoplasm; when associated with R-283; R-335; R-410; R-472 and R-553. 1 Publication
Mutagenesisi472 – 4721K → R: No effect on the recruitment of ADO3 to the cytoplasm; when associated with R-283; R-335; R-410; R-463 and R-553. 1 Publication
Mutagenesisi553 – 5531K → R: No effect on the recruitment of ADO3 to the cytoplasm; when associated with R-283; R-335; R-410; R-463 and R-472. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 611611Adagio protein 2PRO_0000119957Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei82 – 821S-4a-FMN cysteineBy similarity

Post-translational modificationi

FMN binds covalently to cysteine after exposure to blue light and is reversed in the dark.By similarity

Proteomic databases

PaxDbiQ8W420.
PRIDEiQ8W420.

Expressioni

Tissue specificityi

Weakly expressed in seedlings, root tips, stems, leaves, flowers, young siliques, sepals and seeds.2 Publications

Gene expression databases

GenevisibleiQ8W420. AT.

Interactioni

Subunit structurei

Self-associates. Interacts (via Kelch repeats) with AD03, CDF1, CDF2 and CDF3. Interacts with GI, ADO1, SKP1A/ASK1, SKP1B/ASK2, SKP1C/ASK3, SKP1D/ASK4, SKP1E/ASK5, SKP1K/ASK11, SKP1N/ASK14, SKP1TA/ASK20A, SKP1TB/ASK20B, APRR1, APRR5, DI19 and COL1.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDF1Q8W1E32EBI-1015688,EBI-1536051
CDF2Q93ZL53EBI-1015688,EBI-1536103
CDF3Q8LFV33EBI-1015688,EBI-1536119
GIQ9SQI23EBI-1015688,EBI-446380

Protein-protein interaction databases

BioGridi1765. 29 interactions.
DIPiDIP-33897N.
IntActiQ8W420. 16 interactions.
STRINGi3702.AT2G18915.2.

Structurei

3D structure databases

ProteinModelPortaliQ8W420.
SMRiQ8W420. Positions 47-152, 493-606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 11480PASPROSITE-ProRule annotationAdd
BLAST
Domaini118 – 15841PACAdd
BLAST
Domaini196 – 24247F-boxAdd
BLAST
Repeati293 – 34351Kelch 1Add
BLAST
Repeati358 – 39639Kelch 2Add
BLAST
Repeati397 – 44650Kelch 3Add
BLAST
Repeati463 – 51553Kelch 4Add
BLAST
Repeati527 – 57953Kelch 5Add
BLAST

Sequence similaritiesi

Belongs to the ADAGIO family.Curated
Contains 1 F-box domain.Curated
Contains 5 Kelch repeats.Curated
Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiENOG410IKJW. Eukaryota.
ENOG410XRGE. LUCA.
HOGENOMiHOG000238789.
InParanoidiQ8W420.
KOiK12117.
OMAiCAVGNRI.
PhylomeDBiQ8W420.

Family and domain databases

Gene3Di2.120.10.80. 2 hits.
InterProiIPR001810. F-box_dom.
IPR015915. Kelch-typ_b-propeller.
IPR011498. Kelch_2.
IPR000014. PAS.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF07646. Kelch_2. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 1 hit.
SSF81383. SSF81383. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50112. PAS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8W420-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQNQMEWDSD SDLSGGDEVA EDGWFGGDNG AIPFPVGSLP GTAPCGFVVS
60 70 80 90 100
DALEPDNPII YVNTVFEIVT GYRAEEVIGR NCRFLQCRGP FTKRRHPMVD
110 120 130 140 150
STIVAKMRQC LENGIEFQGE LLNFRKDGSP LMNKLRLVPI REEDEITHFI
160 170 180 190 200
GVLLFTDAKI DLGPSPDLSA KEIPRISRSF TSALPIGERN VSRGLCGIFE
210 220 230 240 250
LSDEVIAIKI LSQLTPGDIA SVGCVCRRLN ELTKNDDVWR MVCQNTWGTE
260 270 280 290 300
ATRVLESVPG AKRIGWVRLA REFTTHEATA WRKFSVGGTV EPSRCNFSAC
310 320 330 340 350
AVGNRIVIFG GEGVNMQPMN DTFVLDLGSS SPEWKSVLVS SPPPGRWGHT
360 370 380 390 400
LSCVNGSRLV VFGGYGSHGL LNDVFLLDLD ADPPSWREVS GLAPPIPRSW
410 420 430 440 450
HSSCTLDGTK LIVSGGCADS GALLSDTFLL DLSMDIPAWR EIPVPWTPPS
460 470 480 490 500
RLGHTLTVYG DRKILMFGGL AKNGTLRFRS NDVYTMDLSE DEPSWRPVIG
510 520 530 540 550
YGSSLPGGMA APPPRLDHVA ISLPGGRILI FGGSVAGLDS ASQLYLLDPN
560 570 580 590 600
EEKPAWRILN VQGGPPRFAW GHTTCVVGGT RLVVLGGQTG EEWMLNEAHE
610
LLLATSTTAS T
Length:611
Mass (Da):66,351
Last modified:March 1, 2002 - v1
Checksum:iFDB9F994CBF0D017
GO
Isoform 2 (identifier: Q8W420-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     79-88: Missing.

Show »
Length:601
Mass (Da):65,116
Checksum:i2860CE717F0CBD5F
GO

Sequence cautioni

The sequence AAK27434.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAM14891.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei79 – 8810Missing in isoform 2. 1 PublicationVSP_016048

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF252295 mRNA. Translation: AAK27434.1. Different initiation.
AB038797 mRNA. Translation: BAB83169.1.
AC003673 Genomic DNA. Translation: AAM14891.1. Different initiation.
CP002685 Genomic DNA. Translation: AEC06825.1.
CP002685 Genomic DNA. Translation: AEC06826.1.
AY099709 mRNA. Translation: AAM20560.1.
BT046116 mRNA. Translation: ACI46504.1.
RefSeqiNP_565444.2. NM_127448.2. [Q8W420-2]
NP_849983.1. NM_179652.1. [Q8W420-1]
UniGeneiAt.19440.
At.68004.

Genome annotation databases

EnsemblPlantsiAT2G18915.2; AT2G18915.2; AT2G18915. [Q8W420-1]
GeneIDi816408.
KEGGiath:AT2G18915.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF252295 mRNA. Translation: AAK27434.1. Different initiation.
AB038797 mRNA. Translation: BAB83169.1.
AC003673 Genomic DNA. Translation: AAM14891.1. Different initiation.
CP002685 Genomic DNA. Translation: AEC06825.1.
CP002685 Genomic DNA. Translation: AEC06826.1.
AY099709 mRNA. Translation: AAM20560.1.
BT046116 mRNA. Translation: ACI46504.1.
RefSeqiNP_565444.2. NM_127448.2. [Q8W420-2]
NP_849983.1. NM_179652.1. [Q8W420-1]
UniGeneiAt.19440.
At.68004.

3D structure databases

ProteinModelPortaliQ8W420.
SMRiQ8W420. Positions 47-152, 493-606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi1765. 29 interactions.
DIPiDIP-33897N.
IntActiQ8W420. 16 interactions.
STRINGi3702.AT2G18915.2.

Proteomic databases

PaxDbiQ8W420.
PRIDEiQ8W420.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G18915.2; AT2G18915.2; AT2G18915. [Q8W420-1]
GeneIDi816408.
KEGGiath:AT2G18915.

Organism-specific databases

TAIRiAT2G18915.

Phylogenomic databases

eggNOGiENOG410IKJW. Eukaryota.
ENOG410XRGE. LUCA.
HOGENOMiHOG000238789.
InParanoidiQ8W420.
KOiK12117.
OMAiCAVGNRI.
PhylomeDBiQ8W420.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ8W420.

Gene expression databases

GenevisibleiQ8W420. AT.

Family and domain databases

Gene3Di2.120.10.80. 2 hits.
InterProiIPR001810. F-box_dom.
IPR015915. Kelch-typ_b-propeller.
IPR011498. Kelch_2.
IPR000014. PAS.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF07646. Kelch_2. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 1 hit.
SSF81383. SSF81383. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50112. PAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An Arabidopsis circadian clock component interacts with both CRY1 and phyB."
    Jarillo J.A., Capel J., Tang R.-H., Yang H.-Q., Alonso J.M., Ecker J.R., Cashmore A.R.
    Nature 410:487-490(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  2. "A role for LKP2 in the circadian clock of Arabidopsis."
    Schultz T.F., Kiyosue T., Yanovsky M., Wada M., Kay S.A.
    Plant Cell 13:2659-2670(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INDUCTION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. "Arabidopsis ORF clones."
    de los Reyes C., Quan R., Chen H., Bautista V., Kim C.J., Ecker J.R.
    Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  7. Cited for: GENE FAMILY, NOMENCLATURE.
  8. "Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis."
    Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T.
    J. Exp. Bot. 55:2015-2027(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SKP1A; SKP1B; SKP1C; SKP1D; SKP1E; SKP1K; SKP1N; SKP1TA; SKP1TB; ADO1; AD03; APRR1 AND APRR5, SELF-ASSOCIATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  9. "Identification of LOV KELCH PROTEIN2 (LKP2)-interacting factors that can recruit LKP2 to nuclear bodies."
    Fukamatsu Y., Mitsui S., Yasuhara M., Tokioka Y., Ihara N., Fujita S., Kiyosue T.
    Plant Cell Physiol. 46:1340-1349(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DI19 AND COL1, SUBCELLULAR LOCATION.
  10. "FKF1 F-box protein mediates cyclic degradation of a repressor of CONSTANS in Arabidopsis."
    Imaizumi T., Schultz T.F., Harmon F.G., Ho L.A., Kay S.A.
    Science 309:293-297(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CDF1; CDF2 AND CDF3, DISRUPTION PHENOTYPE.
  11. "ZEITLUPE is a circadian photoreceptor stabilized by GIGANTEA in blue light."
    Kim W.Y., Fujiwara S., Suh S.S., Kim J., Kim Y., Han L., David K., Putterill J., Nam H.G., Somers D.E.
    Nature 449:356-360(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GI.
  12. "LOV KELCH PROTEIN2 and ZEITLUPE repress Arabidopsis photoperiodic flowering under non-inductive conditions, dependent on FLAVIN-BINDING KELCH REPEAT F-BOX1."
    Takase T., Nishiyama Y., Tanihigashi H., Ogura Y., Miyazaki Y., Yamada Y., Kiyosue T.
    Plant J. 67:608-621(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ADO3, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-283; LYS-335; LYS-410; LYS-463; LYS-472 AND LYS-553, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiADO2_ARATH
AccessioniPrimary (citable) accession number: Q8W420
Secondary accession number(s): B5X4Z7, Q9C5S6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 1, 2002
Last modified: May 11, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

'Adagio' means slowly in Italian.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.