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Q8W420 (ADO2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adagio protein 2
Alternative name(s):
F-box only protein 2c
Short name=FBX2c
Flavin-binding kelch repeat F-box protein 1-like protein 1
Short name=FKF1-like protein 1
LOV kelch protein 2
Gene names
Name:ADO2
Synonyms:FKL1, LKP2
Ordered Locus Names:At2g18915
ORF Names:F19F24.11
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length611 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of E3 ubiquitin ligase complexes that plays a central role blue-light-dependent circadian cycles. Acts as a blue-light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO2) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including transition to flowering time, hypocotyl elongation, cotyledons and leaves movement rhythms. APRR1 and APRR5 seem to be substrates of the SCF(ADO1) complex. Ref.2

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Self-associates. Interacts with ADO1, AD03, SKP1A/ASK1, SKP1B/ASK2, SKP1C/ASK3, SKP1D/ASK4, SKP1E/ASK5, SKP1K/ASK11, SKP1N/ASK14, SKP1TA/ASK20A, SKP1TB/ASK20B, APRR1, APRR5, DI19 and COL1. Ref.7 Ref.8

Subcellular location

Nucleus Ref.7 Ref.8.

Tissue specificity

Weakly expressed in seedlings, root tips, stems, leaves, flowers, young siliques, sepals and seeds. Ref.2 Ref.7

Post-translational modification

FMN binds covalently to cysteine after exposure to blue light and is reversed in the dark By similarity.

Miscellaneous

'Adagio' means slowly in Italian.

Sequence similarities

Belongs to the ADAGIO family.

Contains 1 F-box domain.

Contains 5 Kelch repeats.

Contains 1 PAC (PAS-associated C-terminal) domain.

Contains 1 PAS (PER-ARNT-SIM) domain.

Sequence caution

The sequence AAK27434.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAM14891.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8W420-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8W420-2)

The sequence of this isoform differs from the canonical sequence as follows:
     79-88: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 611611Adagio protein 2
PRO_0000119957

Regions

Domain35 – 11480PAS
Domain118 – 15841PAC
Domain196 – 24247F-box
Repeat293 – 34351Kelch 1
Repeat358 – 39639Kelch 2
Repeat397 – 44650Kelch 3
Repeat463 – 51553Kelch 4
Repeat527 – 57953Kelch 5

Amino acid modifications

Modified residue821S-4a-FMN cysteine By similarity

Natural variations

Alternative sequence79 – 8810Missing in isoform 2.
VSP_016048

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: FDB9F994CBF0D017

FASTA61166,351
        10         20         30         40         50         60 
MQNQMEWDSD SDLSGGDEVA EDGWFGGDNG AIPFPVGSLP GTAPCGFVVS DALEPDNPII 

        70         80         90        100        110        120 
YVNTVFEIVT GYRAEEVIGR NCRFLQCRGP FTKRRHPMVD STIVAKMRQC LENGIEFQGE 

       130        140        150        160        170        180 
LLNFRKDGSP LMNKLRLVPI REEDEITHFI GVLLFTDAKI DLGPSPDLSA KEIPRISRSF 

       190        200        210        220        230        240 
TSALPIGERN VSRGLCGIFE LSDEVIAIKI LSQLTPGDIA SVGCVCRRLN ELTKNDDVWR 

       250        260        270        280        290        300 
MVCQNTWGTE ATRVLESVPG AKRIGWVRLA REFTTHEATA WRKFSVGGTV EPSRCNFSAC 

       310        320        330        340        350        360 
AVGNRIVIFG GEGVNMQPMN DTFVLDLGSS SPEWKSVLVS SPPPGRWGHT LSCVNGSRLV 

       370        380        390        400        410        420 
VFGGYGSHGL LNDVFLLDLD ADPPSWREVS GLAPPIPRSW HSSCTLDGTK LIVSGGCADS 

       430        440        450        460        470        480 
GALLSDTFLL DLSMDIPAWR EIPVPWTPPS RLGHTLTVYG DRKILMFGGL AKNGTLRFRS 

       490        500        510        520        530        540 
NDVYTMDLSE DEPSWRPVIG YGSSLPGGMA APPPRLDHVA ISLPGGRILI FGGSVAGLDS 

       550        560        570        580        590        600 
ASQLYLLDPN EEKPAWRILN VQGGPPRFAW GHTTCVVGGT RLVVLGGQTG EEWMLNEAHE 

       610 
LLLATSTTAS T

« Hide

Isoform 2 [UniParc].

Checksum: 2860CE717F0CBD5F
Show »

FASTA60165,116

References

« Hide 'large scale' references
[1]"An Arabidopsis circadian clock component interacts with both CRY1 and phyB."
Jarillo J.A., Capel J., Tang R.-H., Yang H.-Q., Alonso J.M., Ecker J.R., Cashmore A.R.
Nature 410:487-490(2001) [PubMed: 11260718] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"A role for LKP2 in the circadian clock of Arabidopsis."
Schultz T.F., Kiyosue T., Yanovsky M., Wada M., Kay S.A.
Plant Cell 13:2659-2670(2001) [PubMed: 11752379] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INDUCTION.
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[6]"F-box proteins in Arabidopsis."
Xiao W., Jang J.-C.
Trends Plant Sci. 5:454-457(2000) [PubMed: 11077244] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[7]"Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis."
Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T.
J. Exp. Bot. 55:2015-2027(2004) [PubMed: 15310821] [Abstract]
Cited for: INTERACTION WITH SKP1A; SKP1B; SKP1C; SKP1D; SKP1E; SKP1K; SKP1N; SKP1TA; SKP1TB; ADO1; AD03; APRR1 AND APRR5, SELF-ASSOCIATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[8]"Identification of LOV KELCH PROTEIN2 (LKP2)-interacting factors that can recruit LKP2 to nuclear bodies."
Fukamatsu Y., Mitsui S., Yasuhara M., Tokioka Y., Ihara N., Fujita S., Kiyosue T.
Plant Cell Physiol. 46:1340-1349(2005) [PubMed: 15937324] [Abstract]
Cited for: INTERACTION WITH DI19 AND COL1, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF252295 mRNA. Translation: AAK27434.1. Different initiation.
AB038797 mRNA. Translation: BAB83169.1.
AC003673 Genomic DNA. Translation: AAM14891.1. Different initiation.
CP002685 Genomic DNA. Translation: AEC06825.1.
CP002685 Genomic DNA. Translation: AEC06826.1.
AY099709 mRNA. Translation: AAM20560.1.
IPIIPI00524842.
IPI00528666.
RefSeqNP_565444.2. NM_127448.2.
NP_849983.1. NM_179652.1.
UniGeneAt.19440.
At.68004.

3D structure databases

ProteinModelPortalQ8W420.
SMRQ8W420. Positions 35-153, 197-242, 274-408.
ModBaseSearch...

Protein-protein interaction databases

IntActQ8W420. 18 interactions.
STRINGQ8W420.

Proteomic databases

PRIDEQ8W420.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G18915.2; AT2G18915.2; AT2G18915.
GeneID816408.
GenomeReviewsGene locus AT2G18915 in contig CT485783_GR.
KEGGath:AT2G18915.
NMPDRfig|3702.1.peg.8887.

Organism-specific databases

GeneFarm5107. 485.
TAIRAt2g18915.

Phylogenomic databases

GeneTreeEPGT00070000028386.
HOGENOMHBG319046.
InParanoidQ8W420.
OMAEEDEITH.
PhylomeDBQ8W420.
ProtClustDBCLSN2689078.

Gene expression databases

GenevestigatorQ8W420.
GermOnlineAT2G18915. Arabidopsis thaliana.

Family and domain databases

InterProIPR022364. F-box_dom_Skp2-like.
IPR015915. Kelch-typ_b-propeller.
IPR006652. Kelch_1.
IPR011498. Kelch_2.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
[Graphical view]
Gene3DG3DSA:2.120.10.80. Kelch-typ_b-propeller. 1 hit.
KOK12117.
PfamPF01344. Kelch_1. 1 hit.
PF07646. Kelch_2. 2 hits.
PF00989. PAS. 1 hit.
[Graphical view]
SMARTSM00086. PAC. 1 hit.
[Graphical view]
SUPFAMSSF81383. F-box_dom_Skp2-like. 1 hit.
TIGRFAMsTIGR00229. Sensory_box. 1 hit.
PROSITEPS50181. FBOX. False negative.
PS50113. PAC. False negative.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameADO2_ARATH
AccessionPrimary (citable) accession number: Q8W420
Secondary accession number(s): Q9C5S6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 1, 2002
Last modified: November 16, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents