Q8W413 (INV4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-fructofuranosidase, insoluble isoenzyme CWINV4
EC=3.2.1.26

Alternative name(s):
Beta-fructofuranosidase 6
Short name=AtFruct6
Cell wall beta-fructosidase 4
Cell wall invertase 4
Short name=AtcwINV4
Sucrose hydrolase 4

Gene names

Name:	CWINV4
Synonyms:	FRUCT6
Ordered Locus Names:	At2g36190
ORF Names:	F9C22.8

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Subcellular location	Secreted › extracellular space › apoplast Probable. Secreted › cell wall Probable. Note: Associated to the cell wall Probable.
Tissue specificity	Expressed in flowers, and seeds, and, to a lower extent, in seedlings. Ref.5
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nectar secretion Inferred from mutant phenotype. Source: TAIR starch metabolic process Inferred from mutant phenotype. Source: TAIR sucrose catabolic process Inferred from mutant phenotype. Source: TAIR
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from direct assay. Source: TAIR
Molecular function	sucrose alpha-glucosidase activity Inferred from mutant phenotype. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 591	569	Beta-fructofuranosidase, insoluble isoenzyme CWINV4		PRO_0000348350
Regions
	Region	61 – 64	4	Substrate binding By similarity
	Region	123 – 124	2	Substrate binding By similarity
	Region	187 – 188	2	Substrate binding By similarity
Sites
	Active site	64	1	By similarity
	Binding site	80	1	Substrate By similarity
	Binding site	88	1	Substrate By similarity
	Binding site	242	1	Substrate By similarity
	Binding site	276	1	Substrate By similarity
Amino acid modifications
	Glycosylation	145	1	N-linked (GlcNAc...) Potential
	Glycosylation	182	1	N-linked (GlcNAc...) Probable
	Glycosylation	336	1	N-linked (GlcNAc...) Probable
	Glycosylation	472	1	N-linked (GlcNAc...) Potential
	Glycosylation	565	1	N-linked (GlcNAc...) Potential
	Disulfide bond	436 ↔ 484		By similarity
Experimental info
	Sequence conflict	134	1	G → V in AAM61359. Ref.4
	Sequence conflict	158	1	P → R in AAM61359. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8W413 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 2886EE8BC024E511	FASTA	591	67,413
	10 20 30 40 50 60 MAISNVISVL LLLLVLINLS NQNIKGIDAF HQIYEELQSE SVESVNHLHR PSFHFQPPKH 70 80 90 100 110 120 WINDPNGPVY YKGLYHLFYQ YNTKGAVWGN IIWAHSVSKD LVNWEALEPA LSPSKWFDIG 130 140 150 160 170 180 GTWSGSITIV PGKGPIILYT GVNQNETQLQ NYAIPEDPSD PYLRKWIKPD DNPIAIPDYT 190 200 210 220 230 240 MNGSAFRDPT TAWFSKDGHW RTVVGSKRKR RGIAYIYRSR DFKHWVKAKH PVHSKQSTGM 250 260 270 280 290 300 WECPDFFPVS LTDFRNGLDL DYVGPNTKHV LKVSLDITRY EYYTLGKYDL KKDRYIPDGN 310 320 330 340 350 360 TPDGWEGLRF DYGNFYASKT FFDYKKNRRI LWGWANESDT VEDDILKGWA GLQVIPRTVL 370 380 390 400 410 420 LDSSKKQLVF WPVEEIESLR GNYVRMNNHD IKMGQRIEVK GITPAQADVE VTFYVGSLEK 430 440 450 460 470 480 AEIFDPSFTW KPLELCNIKG SNVRGGVGPF GLITLATPDL EEYTPVFFRV FNDTKTHKPK 490 500 510 520 530 540 VLMCSDARPS SLKQDTGLLA KDRMYKPSFA GFVDVDMADG RISLRSLIDH SVVESFGALG 550 560 570 580 590 KTVITSRVYP VKAVKENAHL YVFNNGTQTV TIESLNAWNM DRPLQMNDGA L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"acid invertase gene No. 6 from Arabidopsis." Toyomasu T., Sasaki S., Mitsuhasi W. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Roles of cell-wall invertases and monosaccharide transporters in the growth and development of Arabidopsis." Sherson S.M., Alford H.L., Forbes S.M., Wallace G., Smith S.M. J. Exp. Bot. 54:525-531(2003) [PubMed: 12508063] [Abstract] Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB049617 mRNA. Translation: BAB83031.1. AC006921 Genomic DNA. Translation: AAD21446.2. AC007135 Genomic DNA. Translation: AAM15406.1. CP002685 Genomic DNA. Translation: AEC09214.1. AY084792 mRNA. Translation: AAM61359.1.
IPI	IPI00545558.
PIR	G84777.
RefSeq	NP_565837.1. NM_129177.2.
UniGene	At.14109.
3D structure databases
ProteinModelPortal	Q8W413.
SMR	Q8W413. Positions 46-587.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8W413.
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Proteomic databases
PRIDE	Q8W413.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G36190.1; AT2G36190.1; AT2G36190.
GeneID	818191.
GenomeReviews	Gene locus AT2G36190 in contig CT485783_GR.
KEGG	ath:AT2G36190.
NMPDR	fig|3702.1.peg.10722.
Organism-specific databases
TAIR	At2g36190.
Phylogenomic databases
eggNOG	KOG0228.
GeneTree	EPGT00050000007871.
HOGENOM	HBG317462.
InParanoid	Q8W413.
OMA	RYEYYTI.
PhylomeDB	Q8W413.
ProtClustDB	CLSN2688723.
Gene expression databases
ArrayExpress	Q8W413.
Genevestigator	Q8W413.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_43_beta-prop. [Graphical view]
Gene3D	G3DSA:2.115.10.20. Glyco_hydro_43_beta-prop. 1 hit.
KO	K01193.
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INV4_ARATH

Accession

Primary (citable) accession number: Q8W413
Secondary accession number(s): Q8L5V2, Q9SJN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	March 1, 2002
Last modified:	November 16, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program