ID   PORB_ORYSJ              Reviewed;         402 AA.
AC   Q8W3D9; A0A0P0XWE3; Q337H5; Q7XD32;
DT   26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   24-JAN-2024, entry version 126.
DE   RecName: Full=Protochlorophyllide reductase B, chloroplastic;
DE            Short=PCR B;
DE            EC=1.3.1.33;
DE   AltName: Full=NADPH-protochlorophyllide oxidoreductase B;
DE            Short=POR B;
DE   Flags: Precursor;
GN   Name=PORB; OrderedLocusNames=Os10g0496900, LOC_Os10g35370;
GN   ORFNames=OSJNBa0017E08.8;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12791992; DOI=10.1126/science.1083523;
RA   Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S.,
RA   Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D.,
RA   Oates R., Palmer M., Pries G., Gibson J., Anderson H., Paradkar M.,
RA   Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F.,
RA   Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M.,
RA   Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R.,
RA   Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F.,
RA   Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D.,
RA   Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R.,
RA   Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K.,
RA   Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S.,
RA   Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S.,
RA   Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R.,
RA   Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M.,
RA   Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R.,
RA   Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E.,
RA   Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
RT   "In-depth view of structure, activity, and evolution of rice chromosome
RT   10.";
RL   Science 300:1566-1569(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Phototransformation of protochlorophyllide (Pchlide) to
CC       chlorophyllide (Chlide).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=chlorophyllide a + NADP(+) = H(+) + NADPH +
CC         protochlorophyllide a; Xref=Rhea:RHEA:11132, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:83348,
CC         ChEBI:CHEBI:83350; EC=1.3.1.33;
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q8W3D9-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8W3D9-2; Sequence=VSP_037403;
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. POR subfamily. {ECO:0000305}.
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DR   EMBL; AC068923; AAL58280.1; -; Genomic_DNA.
DR   EMBL; DP000086; AAP54438.1; -; Genomic_DNA.
DR   EMBL; DP000086; ABB47824.1; -; Genomic_DNA.
DR   EMBL; AP008216; BAF26869.1; -; Genomic_DNA.
DR   EMBL; AP014966; BAT11485.1; -; Genomic_DNA.
DR   EMBL; AK068143; BAG90777.1; -; mRNA.
DR   RefSeq; XP_015614945.1; XM_015759459.1.
DR   AlphaFoldDB; Q8W3D9; -.
DR   SMR; Q8W3D9; -.
DR   STRING; 39947.Q8W3D9; -.
DR   PaxDb; 39947-Q8W3D9; -.
DR   EnsemblPlants; Os10t0496900-02; Os10t0496900-02; Os10g0496900. [Q8W3D9-1]
DR   GeneID; 4349004; -.
DR   Gramene; Os10t0496900-02; Os10t0496900-02; Os10g0496900. [Q8W3D9-1]
DR   KEGG; osa:4349004; -.
DR   InParanoid; Q8W3D9; -.
DR   OrthoDB; 713at2759; -.
DR   BRENDA; 1.3.1.33; 8948.
DR   UniPathway; UPA00668; -.
DR   Proteomes; UP000000763; Chromosome 10.
DR   Proteomes; UP000059680; Chromosome 10.
DR   ExpressionAtlas; Q8W3D9; baseline and differential.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0016630; F:protochlorophyllide reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   CDD; cd09810; LPOR_like_SDR_c_like; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR005979; Prochl_reduct.
DR   InterPro; IPR002347; SDR_fam.
DR   NCBIfam; TIGR01289; LPOR; 1.
DR   PANTHER; PTHR44419; PROTOCHLOROPHYLLIDE REDUCTASE C, CHLOROPLASTIC; 1.
DR   PANTHER; PTHR44419:SF15; PROTOCHLOROPHYLLIDE REDUCTASE C, CHLOROPLASTIC; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Chlorophyll biosynthesis; Chloroplast; NADP;
KW   Oxidoreductase; Photosynthesis; Plastid; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..402
FT                   /note="Protochlorophyllide reductase B, chloroplastic"
FT                   /id="PRO_0000376077"
FT   VAR_SEQ         1..114
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:12869764"
FT                   /id="VSP_037403"
SQ   SEQUENCE   402 AA;  42766 MW;  749869F7316DFAAA CRC64;
     MALQAATTTS FLPSALSARK EGAVKDSAFL GVRLGDGLKL ETSALGLRTK RVSTSSVAIR
     AQASAAVSSP TVTPASPSGK QTLRKGTAVI TGASSGLGLA TAKALAETGR WHVVMGCRDF
     LKASRAAKAA GMEKGSYTIV HLDLASLDSV RQFVANVRRL EMPVDVVVCN AAVYQPTAKQ
     PSFTADGFEM SVGVNHLGHF LLARELLADL TSSDYPSKRL IIVGSITGNT NTLAGNVPPK
     ANLGDLRGLA SGLDGVSSSA MIDGGEFDGA KAYKDSKVCN MLTMQEFHRR YHGETGVTFA
     SLYPGCIATT GLFREHVPLF RLLFPPFQKY ITKGYVSEEE AGKRLAQVVS DPSLTKSGVY
     WSWNNNSASF ENQLSEEASD PEKAKKVWEL SEKLVGLADH DQ
//