Q8W3D9 (PORB_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protochlorophyllide reductase B, chloroplastic
Short name=PCR B
EC=1.3.1.33

Alternative name(s):
NADPH-protochlorophyllide oxidoreductase B
Short name=POR B

Gene names

Name:	PORB
Ordered Locus Names:	Os10g0496900, LOC_Os10g35370
ORF Names:	OSJNBa0017E08.8

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).
Catalytic activity	Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis.
Subcellular location	Plastid › chloroplast By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. POR subfamily.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW response to ethylene stimulus Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	chloroplast envelope Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast thylakoid membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	NADPH dehydrogenase activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene nucleotide binding Inferred from electronic annotation. Source: InterPro protochlorophyllide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8W3D9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Derived from EST data. No experimental confirmation available.

Isoform 2 (identifier: Q8W3D9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-114: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 402		Protochlorophyllide reductase B, chloroplastic		PRO_0000376077
Natural variations
	Alternative sequence	1 – 114	114	Missing in isoform 2.		VSP_037403

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 749869F7316DFAAA	FASTA	402	42,766
	10 20 30 40 50 60 MALQAATTTS FLPSALSARK EGAVKDSAFL GVRLGDGLKL ETSALGLRTK RVSTSSVAIR 70 80 90 100 110 120 AQASAAVSSP TVTPASPSGK QTLRKGTAVI TGASSGLGLA TAKALAETGR WHVVMGCRDF 130 140 150 160 170 180 LKASRAAKAA GMEKGSYTIV HLDLASLDSV RQFVANVRRL EMPVDVVVCN AAVYQPTAKQ 190 200 210 220 230 240 PSFTADGFEM SVGVNHLGHF LLARELLADL TSSDYPSKRL IIVGSITGNT NTLAGNVPPK 250 260 270 280 290 300 ANLGDLRGLA SGLDGVSSSA MIDGGEFDGA KAYKDSKVCN MLTMQEFHRR YHGETGVTFA 310 320 330 340 350 360 SLYPGCIATT GLFREHVPLF RLLFPPFQKY ITKGYVSEEE AGKRLAQVVS DPSLTKSGVY 370 380 390 400 WSWNNNSASF ENQLSEEASD PEKAKKVWEL SEKLVGLADH DQ
	Isoform 2 [UniParc]. Checksum: 9D0C3CCC97655D85	FASTA	288	31,377
	10 20 30 40 50 60 MGCRDFLKAS RAAKAAGMEK GSYTIVHLDL ASLDSVRQFV ANVRRLEMPV DVVVCNAAVY 70 80 90 100 110 120 QPTAKQPSFT ADGFEMSVGV NHLGHFLLAR ELLADLTSSD YPSKRLIIVG SITGNTNTLA 130 140 150 160 170 180 GNVPPKANLG DLRGLASGLD GVSSSAMIDG GEFDGAKAYK DSKVCNMLTM QEFHRRYHGE 190 200 210 220 230 240 TGVTFASLYP GCIATTGLFR EHVPLFRLLF PPFQKYITKG YVSEEEAGKR LAQVVSDPSL 250 260 270 280 TKSGVYWSWN NNSASFENQL SEEASDPEKA KKVWELSEKL VGLADHDQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"In-depth view of structure, activity, and evolution of rice chromosome 10." Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S., Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D., Oates R., Palmer M., Pries G., Gibson J., Anderson H. , Paradkar M., Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F., Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M., Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R., Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F., Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D., Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R., Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K., Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S., Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S., Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R., Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M., Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R., Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E., Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A. Science 300:1566-1569(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC068923 Genomic DNA. Translation: AAL58280.1. DP000086 Genomic DNA. Translation: AAP54438.1. DP000086 Genomic DNA. Translation: ABB47824.1. AP008216 Genomic DNA. Translation: BAF26869.1. AK068143 mRNA. Translation: BAG90777.1.
RefSeq	NP_001064955.1. NM_001071490.1.
UniGene	Os.88103.
3D structure databases
ProteinModelPortal	Q8W3D9.
ModBase	Search...
Protein-protein interaction databases
STRING	39947.LOC_Os10g35370.1.
Proteomic databases
PaxDb	Q8W3D9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os10g35370.1; LOC_Os10g35370.1; LOC_Os10g35370.
GeneID	4349004.
KEGG	dosa:Os10t0496900-01. osa:4349004.
Organism-specific databases
Gramene	Q8W3D9.
Phylogenomic databases
eggNOG	COG1028.
KO	K00218.
OMA	WSWNNNS.
ProtClustDB	PLN00015.
Enzyme and pathway databases
UniPathway	UPA00668.
Gene expression databases
ArrayExpress	Q8W3D9.
Family and domain databases
Gene3D	3.40.50.720. 2 hits.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR005979. Prochl_reduct. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
TIGRFAMs	TIGR01289. LPOR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PORB_ORYSJ

Accession

Primary (citable) accession number: Q8W3D9
Secondary accession number(s): Q337H5, Q7XD32

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	March 1, 2002
Last modified:	April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program