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Reviewed, UniProtKB/Swiss-Prot Q8W3D9 (PORB_ORYSJ)

Last modified September 22, 2009. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Protochlorophyllide reductase B, chloroplastic
      Short name=PCR B
    EC=1.3.1.33
Alternative name(s):
    NADPH-protochlorophyllide oxidoreductase B
      Short name=POR B
Gene names
Name: PORB
Ordered Locus Names: Os10g0496900, LOC_Os10g35370
ORF Names: OSJNBa0017E08.8
OrganismOryza sativa subsp. japonica (Rice)
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length402 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).

Catalytic activity

Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.

Pathway

Porphyrin biosynthesis; chlorophyll biosynthesis.

Subcellular location

Plastidchloroplast By similarity.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family. POR subfamily.

Ontologies

Keywords
   Biological processChlorophyll biosynthesis
Photosynthesis
   Cellular componentChloroplast
Plastid
   Coding sequence diversityAlternative splicing
   DomainTransit peptide
   LigandNADP
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processchlorophyll biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

photosynthesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionbinding

Inferred from electronic annotation. Source: InterPro

protochlorophyllide reductase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8W3D9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Derived from EST data. No experimental confirmation available.
Isoform 2 (identifier: Q8W3D9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-114: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 402Protochlorophyllide reductase B, chloroplasticPRO_0000376077

Natural variations

Alternative sequence1 – 114114Missing in isoform 2.
VSP_037403

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 749869F7316DFAAA

FASTA40242,766
        10         20         30         40         50         60 
MALQAATTTS FLPSALSARK EGAVKDSAFL GVRLGDGLKL ETSALGLRTK RVSTSSVAIR 

        70         80         90        100        110        120 
AQASAAVSSP TVTPASPSGK QTLRKGTAVI TGASSGLGLA TAKALAETGR WHVVMGCRDF 

       130        140        150        160        170        180 
LKASRAAKAA GMEKGSYTIV HLDLASLDSV RQFVANVRRL EMPVDVVVCN AAVYQPTAKQ 

       190        200        210        220        230        240 
PSFTADGFEM SVGVNHLGHF LLARELLADL TSSDYPSKRL IIVGSITGNT NTLAGNVPPK 

       250        260        270        280        290        300 
ANLGDLRGLA SGLDGVSSSA MIDGGEFDGA KAYKDSKVCN MLTMQEFHRR YHGETGVTFA 

       310        320        330        340        350        360 
SLYPGCIATT GLFREHVPLF RLLFPPFQKY ITKGYVSEEE AGKRLAQVVS DPSLTKSGVY 

       370        380        390        400 
WSWNNNSASF ENQLSEEASD PEKAKKVWEL SEKLVGLADH DQ 

« Hide

Isoform 2.

Checksum: 9D0C3CCC97655D85
Show »

FASTA28831,377

References

« Hide 'large scale' references
[1]"In-depth view of structure, activity, and evolution of rice chromosome 10."
Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S., Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D., Oates R., Palmer M., Pries G., Gibson J., Anderson H. expand/collapse author list , Paradkar M., Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F., Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M., Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R., Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F., Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D., Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R., Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K., Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S., Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S., Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R., Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M., Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R., Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E., Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.
Science 300:1566-1569(2003) [PubMed: 12791992] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed: 16100779] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana."
The rice annotation project (RAP)
Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[4]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed: 12869764] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Nipponbare.

Cross-references

Sequence databases

AC068923 Genomic DNA. Translation: AAL58280.1.
DP000086 Genomic DNA. Translation: AAP54438.1.
DP000086 Genomic DNA. Translation: ABB47824.1.
AP008216 Genomic DNA. Translation: BAF26869.1.
AK068143 mRNA. Translation: BAG90777.1.
RefSeqNP_001064955.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID4349004.
GenomeReviewsGene locus PORB in contig AP008216_GR.
KEGGosa:4349004.
NMPDRfig|39947.1.peg.13659.

Organism-specific databases

GrameneQ8W3D9.

Family and domain databases

InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR005979. Prochl_reduct.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR19410. ADH_short_C2. 1 hit.
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
TIGRFAMsTIGR01289. LPOR. 1 hit.
ProtoNetSearch...

Entry information

Entry namePORB_ORYSJ
AccessionPrimary (citable) accession number: Q8W3D9
Secondary accession number(s): Q337H5, Q7XD32
Entry history
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: March 1, 2002
Last modified: September 22, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents