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Protein

Serine acetyltransferase 4

Gene

SAT4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine.

Enzyme regulationi

Feedback inhibitions by L-Ser and acetyl-CoA.1 Publication

Kineticsi

  1. KM=39.5 mM for L-Ser (at pH 8 and 30 degrees Celsius)1 Publication
  2. KM=45.1 mM for acetyl-CoA (at pH 8 and 30 degrees Celsius)1 Publication

    Pathwayi: L-cysteine biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes L-cysteine from L-serine.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Serine acetyltransferase 3, mitochondrial (SAT3), Serine acetyltransferase 5 (SAT5), Serine acetyltransferase 1, chloroplastic (SAT1), Serine acetyltransferase 2 (SAT2), Serine acetyltransferase 4 (SAT4)
    2. Bifunctional L-3-cyanoalanine synthase/cysteine synthase D2 (CYSD2), Bifunctional cystathionine gamma-lyase/cysteine synthase (DES1), Cysteine synthase, mitochondrial (OASC), Bifunctional L-3-cyanoalanine synthase/cysteine synthase D1 (CYSD1), Cysteine synthase, chloroplastic/chromoplastic (OASB), Cysteine synthase 1 (OASA1)
    This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

    GO - Molecular functioni

    • serine O-acetyltransferase activity Source: TAIR

    GO - Biological processi

    • cysteine biosynthetic process Source: GO_Central
    • cysteine biosynthetic process from serine Source: InterPro
    • sulfate assimilation Source: TAIR

    Keywordsi

    Molecular functionAcyltransferase, Transferase
    Biological processAmino-acid biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00136; UER00199.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine acetyltransferase 4 (EC:2.3.1.30)
    Short name:
    AtSAT-4
    Short name:
    AtSERAT3;2
    Gene namesi
    Name:SAT4
    Ordered Locus Names:At4g35640
    ORF Names:F8D20.150
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    TAIRilocus:2127978. AT4G35640.

    Subcellular locationi

    • Cytoplasm 1 Publication

    GO - Cellular componenti

    • cytosol Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000686921 – 355Serine acetyltransferase 4Add BLAST355

    Proteomic databases

    PaxDbiQ8W2B8.

    PTM databases

    iPTMnetiQ8W2B8.

    Expressioni

    Tissue specificityi

    Localized in vascular tissues, particularly in phloem.1 Publication

    Inductioni

    By cadmium (Cd). Induced in roots and shoots under sulfur-deficient conditions.1 Publication

    Gene expression databases

    ExpressionAtlasiQ8W2B8. baseline and differential.

    Interactioni

    Subunit structurei

    Homomultimer.By similarity

    Protein-protein interaction databases

    BioGridi14998. 2 interactors.
    MINTiMINT-8061727.
    STRINGi3702.AT4G35640.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8W2B8.
    SMRiQ8W2B8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi12 – 19Poly-Ser8

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG410INAF. Eukaryota.
    COG1045. LUCA.
    HOGENOMiHOG000049437.
    InParanoidiQ8W2B8.
    KOiK00640.
    OMAiMIVSRNF.
    OrthoDBiEOG09360HSO.
    PhylomeDBiQ8W2B8.

    Family and domain databases

    InterProiView protein in InterPro
    IPR001451. Hexapep.
    IPR018357. Hexapep_transf_CS.
    IPR010493. Ser_AcTrfase_N.
    IPR005881. Ser_O-AcTrfase.
    IPR011004. Trimer_LpxA-like.
    PfamiView protein in Pfam
    PF00132. Hexapep. 1 hit.
    PF06426. SATase_N. 1 hit.
    SMARTiView protein in SMART
    SM00971. SATase_N. 1 hit.
    SUPFAMiSSF51161. SSF51161. 1 hit.
    TIGRFAMsiTIGR01172. cysE. 1 hit.
    PROSITEiView protein in PROSITE
    PS00101. HEXAPEP_TRANSFERASES. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8W2B8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MACINGENRD FSSSSSLSSL PMIVSRNFSA RDDGETGDEF PFERIFPVYA
    60 70 80 90 100
    RGTLNPVADP VLLDFTNSSY DPIWDSIREE AKLEAEEEPV LSSFLYASIL
    110 120 130 140 150
    SHDCLEQALS FVLANRLQNP TLLATQLMDI FCNVMVHDRG IQSSIRLDVQ
    160 170 180 190 200
    AFKDRDPACL SYSSAILHLK GYLALQAYRV AHKLWKQGRK LLALALQSRV
    210 220 230 240 250
    SEVFGIDIHP AARIGKGILL DHGTGVVIGE TAVIGDRVSI LHGVTLGGTG
    260 270 280 290 300
    KETGDRHPNI GDGALLGACV TILGNIKIGA GAMVAAGSLV LKDVPSHSMV
    310 320 330 340 350
    AGNPAKLIGF VDEQDPSMTM EHDATREFFQ NVAVAYRETI PNGSSVSGSC

    RERRH
    Length:355
    Mass (Da):38,424
    Last modified:March 1, 2002 - v1
    Checksum:i3BF39845776C0EC7
    GO

    Sequence cautioni

    The sequence CAA20034 differs from that shown. Reason: Erroneous gene model prediction.Curated
    The sequence CAB80280 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF331847 Genomic DNA. Translation: AAL37489.1.
    AL031135 Genomic DNA. Translation: CAA20034.1. Sequence problems.
    AL161587 Genomic DNA. Translation: CAB80280.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE86543.1.
    BT004080 mRNA. Translation: AAO42107.1.
    BT005047 mRNA. Translation: AAO50580.1.
    PIRiT04669.
    RefSeqiNP_195289.3. NM_119729.4.
    UniGeneiAt.19734.
    At.71197.

    Genome annotation databases

    EnsemblPlantsiAT4G35640.1; AT4G35640.1; AT4G35640.
    GeneIDi829716.
    GrameneiAT4G35640.1; AT4G35640.1; AT4G35640.
    KEGGiath:AT4G35640.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF331847 Genomic DNA. Translation: AAL37489.1.
    AL031135 Genomic DNA. Translation: CAA20034.1. Sequence problems.
    AL161587 Genomic DNA. Translation: CAB80280.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE86543.1.
    BT004080 mRNA. Translation: AAO42107.1.
    BT005047 mRNA. Translation: AAO50580.1.
    PIRiT04669.
    RefSeqiNP_195289.3. NM_119729.4.
    UniGeneiAt.19734.
    At.71197.

    3D structure databases

    ProteinModelPortaliQ8W2B8.
    SMRiQ8W2B8.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi14998. 2 interactors.
    MINTiMINT-8061727.
    STRINGi3702.AT4G35640.1.

    PTM databases

    iPTMnetiQ8W2B8.

    Proteomic databases

    PaxDbiQ8W2B8.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT4G35640.1; AT4G35640.1; AT4G35640.
    GeneIDi829716.
    GrameneiAT4G35640.1; AT4G35640.1; AT4G35640.
    KEGGiath:AT4G35640.

    Organism-specific databases

    AraportiAT4G35640.
    TAIRilocus:2127978. AT4G35640.

    Phylogenomic databases

    eggNOGiENOG410INAF. Eukaryota.
    COG1045. LUCA.
    HOGENOMiHOG000049437.
    InParanoidiQ8W2B8.
    KOiK00640.
    OMAiMIVSRNF.
    OrthoDBiEOG09360HSO.
    PhylomeDBiQ8W2B8.

    Enzyme and pathway databases

    UniPathwayiUPA00136; UER00199.

    Miscellaneous databases

    PROiPR:Q8W2B8.

    Gene expression databases

    ExpressionAtlasiQ8W2B8. baseline and differential.

    Family and domain databases

    InterProiView protein in InterPro
    IPR001451. Hexapep.
    IPR018357. Hexapep_transf_CS.
    IPR010493. Ser_AcTrfase_N.
    IPR005881. Ser_O-AcTrfase.
    IPR011004. Trimer_LpxA-like.
    PfamiView protein in Pfam
    PF00132. Hexapep. 1 hit.
    PF06426. SATase_N. 1 hit.
    SMARTiView protein in SMART
    SM00971. SATase_N. 1 hit.
    SUPFAMiSSF51161. SSF51161. 1 hit.
    TIGRFAMsiTIGR01172. cysE. 1 hit.
    PROSITEiView protein in PROSITE
    PS00101. HEXAPEP_TRANSFERASES. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiSAT4_ARATH
    AccessioniPrimary (citable) accession number: Q8W2B8
    Secondary accession number(s): O81795
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
    Last sequence update: March 1, 2002
    Last modified: April 12, 2017
    This is version 92 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.