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Protein

DIMBOA UDP-glucosyltransferase BX8

Gene

Bx8

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Glucosyltransferase involved in the last step of benzoxazinoid glucoside biosynthesis. Catalyzes the glucosylation of hydroxamic acids utilizing UDP-glucose as glucose doner, reducing the toxicity of these natural insecticides for storage. Can use DIMBOA and DIBOA as substrates, HMBOA (2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one) and HBOA (2-hydroxy-2H-1,4-benzoxazin-3(4H)-one) with a lower efficiency, but not indole acetic acid or quercitin.1 Publication

Catalytic activityi

UDP-alpha-D-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one = UDP + (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside.2 Publications
UDP-alpha-D-glucose + 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one = UDP + (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside.2 Publications

Cofactori

Mg2+1 Publication, Ca2+1 Publication

Kineticsi

kcat is 22.7 sec(-1) for DIMBOA. kcat is 12.5 sec(-1) for DIBOA. kcat is 22.6 sec(-1) for UDPG.
  1. KM=81 µM for 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA)2 Publications
  2. KM=61 µM for 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one (DIBOA)2 Publications
  3. KM=81 µM for uridine 5'-diphosphoglucose (UDPG) with DIMBOA as2 Publications

    pH dependencei

    Optimum pH is 8.5.2 Publications

    Temperature dependencei

    Optimum temperature is 45 degrees Celsius.2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei282UDP-glucoseBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-10602.
    SABIO-RKiQ8W2B7.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DIMBOA UDP-glucosyltransferase BX8 (EC:2.4.1.2022 Publications)
    Alternative name(s):
    2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucosyltransferase BX8
    Protein BENZOXAZINLESS 8
    Gene namesi
    Name:Bx8
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
    Proteomesi
    • UP000007305 Componenti: Unplaced

    Organism-specific databases

    MaizeGDBi9021865.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004153061 – 459DIMBOA UDP-glucosyltransferase BX8Add BLAST459

    Proteomic databases

    PRIDEiQ8W2B7.

    Expressioni

    Tissue specificityi

    Expressed at the same levels in roots and shoots.1 Publication

    Developmental stagei

    Highly expressed in young seedlings up to 4 days after imbibition.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi4577.GRMZM2G085054_P01.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8W2B7.
    SMRiQ8W2B7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni340 – 342UDP-glucose bindingBy similarity3
    Regioni357 – 365UDP-glucose bindingBy similarity9
    Regioni379 – 382UDP-glucose bindingBy similarity4

    Sequence similaritiesi

    Belongs to the UDP-glycosyltransferase family.Curated

    Phylogenomic databases

    HOGENOMiHOG000237564.

    Family and domain databases

    InterProiView protein in InterPro
    IPR002213. UDP_glucos_trans.
    IPR035595. UDP_glycos_trans_CS.
    PANTHERiPTHR11926. PTHR11926. 1 hit.
    PfamiView protein in Pfam
    PF00201. UDPGT. 1 hit.
    PROSITEiView protein in PROSITE
    PS00375. UDPGT. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8W2B7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAASCGGRVV VFPFPFQGHF NPVMRLARAL HARGVGITVF HTAGARAPDP
    60 70 80 90 100
    ADYPADYRFV PVPVEVAPEL MASEDIAAIV TALNAACEAP FRDRLSALLS
    110 120 130 140 150
    AADGEAGEAG GRVRCVLTDV SWDAVLSAAR GLGVPALGVM TASAATFRVY
    160 170 180 190 200
    MAYRTLVDKG YLPVREERKD DAVAELPPYR VKDLLRHETC DLEEFADLLG
    210 220 230 240 250
    RVIAAARLSS GLIFHTFPFI EAGTLGEIRD DMSVPVYAVA PLNKLVPAAT
    260 270 280 290 300
    ASLHGEVQAD RGCLRWLDAQ RARSVLYVSF GSMAAMDPHE FVELAWGLAD
    310 320 330 340 350
    AGRPFVWVVR PNLIRGFESG ALPDGVEDRV RGRGVVVSWA PQEEVLAHPA
    360 370 380 390 400
    VGGFFTHCGW NSTVEAVSEG VPMICHPRHG DQYGNARYVC HVWKVGTEVA
    410 420 430 440 450
    GDQLERGEIK AAIDRLMGGS EEGEGIRKRM NELKIAADKG IDESAGSDLT

    NLVHLINSY
    Length:459
    Mass (Da):49,470
    Last modified:March 1, 2002 - v1
    Checksum:i0F5AE7AAA56AD3E7
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF331854 Genomic DNA. Translation: AAL57037.1.
    UniGeneiZm.67050.

    Similar proteinsi

    Entry informationi

    Entry nameiBX8_MAIZE
    AccessioniPrimary (citable) accession number: Q8W2B7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: March 1, 2002
    Last modified: November 22, 2017
    This is version 63 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families