Reviewed, UniProtKB/Swiss-Prot Q8W250 (DXR_ORYSJ)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose 5-phosphate reductoisomerase, chloroplastic
      Short name=1-deoxyxylulose-5-phosphate reductoisomerase
      Short name=DXP reductoisomerase
    EC=1.1.1.267
Alternative name(s):
    2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	DXR
Ordered Locus Names:	Os01g0106900, LOC_Os01g01710
ORF Names:	P0005A05.24, P0482C06.2

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity.
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH.
Cofactor	Divalent cation By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 49	49	Chloroplast Potential
	Chain	50 – 473	424	1-deoxy-D-xylulose 5-phosphate reductoisomerase, chloroplastic		PRO_0000247469
Regions
	Nucleotide binding	82 – 111	30	NADP By similarity
Sites
	Metal binding	225	1	Divalent metal cation By similarity
	Metal binding	227	1	Divalent metal cation By similarity
	Metal binding	296	1	Divalent metal cation By similarity
Experimental info
	Sequence conflict	128	1	L → H in AAL37560. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8W250-1 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: 9933D9D8C3D1FC49	FASTA	473	51,473
	10 20 30 40 50 60 MALKVVSFPG DLAAVSFLDS NRGGAFNQLK VDLPFQTRDR RAVSLRRTCC SMQQAPPPAW 70 80 90 100 110 120 PGRAVVEPGR RSWDGPKPIS IVGSTGSIGT QTLDIVAENP DKFRVVALAA GSNVTLLADQ 130 140 150 160 170 180 VKTFKPKLVA VRNESLVDEL KEALADCDWK PEIIPGEQGV IEVARHPDAV TVVTGIVGCA 190 200 210 220 230 240 GLKPTVAAIE AGKDIALANK ETLIAGGPFV LPLAQKHKVK ILPADSEHSA IFQCIQGLPE 250 260 270 280 290 300 GALRRIILTA SGGAFRDWPV DKLKEVKVAD ALKHPNWNMG KKITVDSATL FNKGLEVIEA 310 320 330 340 350 360 HYLFGAEYDD IEIVIHPQSI IHSMIETQDS SVLAQLGWPD MRIPILYTMS WPDRIYCSEV 370 380 390 400 410 420 TWPRLDLCKL GSLTFKAPDN VKYPSMDLAY AAGRAGGTMT GVLSAANEKA VELFIDEKIG 430 440 450 460 470 YLDIFKVVEL TCDAHRNELV TRPSLEEIIH YDLWAREYAA SLQPSTGLSP VPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR), catalyzing the first committed step of the mevalonate-independent pathway for IPP biosynthesis." Carretero-Paulet L., Boronat A., Campos N. Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF367205 mRNA. Translation: AAL37560.1. AP002863 Genomic DNA. Translation: BAB16915.1. AP002845 Genomic DNA. Translation: BAB78606.1.
RefSeq	NP_001041780.1.
UniGene	Os.1005
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4326153.
KEGG	osa:4326153.
NMPDR	fig|39947.1.peg.796.
Organism-specific databases
Gramene	Q8W250.
Family and domain databases
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 1 hit. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
TIGRFAMs	TIGR00243. Dxr. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_ORYSJ

Accession

Primary (citable) accession number: Q8W250
Secondary accession number(s): Q9FTN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	November 24, 2009
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)