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Q8W1L6

- MFP_ORYSJ

UniProt

Q8W1L6 - MFP_ORYSJ

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Protein

Peroxisomal fatty acid beta-oxidation multifunctional protein

Gene

MFP

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Multifunctional enzyme involved in fatty acid beta-oxidation. Also binds to RNA and microtubules. Possible role in subcellular mRNA localization and RNA-cytoskeleton interactions.1 Publication1 Publication

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.1 Publication
(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.1 Publication
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.By similarity1 Publication
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.1 Publication

Pathwayi

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydratase activity Source: UniProtKB
  2. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-EC
  3. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB
  4. coenzyme binding Source: EnsemblPlants/Gramene
  5. dodecenoyl-CoA delta-isomerase activity Source: UniProtKB
  6. enoyl-CoA hydratase activity Source: UniProtKB
  7. microtubule binding Source: UniProtKB
  8. mRNA binding Source: UniProtKB

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB
  2. flower development Source: EnsemblPlants/Gramene
  3. jasmonic acid biosynthetic process Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

NAD, RNA-binding

Enzyme and pathway databases

UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal fatty acid beta-oxidation multifunctional protein
Short name:
MFP
Including the following 2 domains:
Enoyl-CoA hydratase/3-2-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3, EC:5.3.3.8)
3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
Gene namesi
Name:MFP
Ordered Locus Names:Os02g0274100, LOC_Os02g17390
ORF Names:P0413A11.18
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 2

Organism-specific databases

GrameneiQ8W1L6.

Subcellular locationi

Peroxisome Curated. Cytoplasmcytoskeleton Curated

GO - Cellular componenti

  1. cell wall Source: EnsemblPlants/Gramene
  2. chloroplast Source: EnsemblPlants/Gramene
  3. microtubule Source: UniProtKB-KW
  4. peroxisome Source: UniProtKB
  5. plasmodesma Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 726726Peroxisomal fatty acid beta-oxidation multifunctional proteinPRO_0000109252Add
BLAST

Proteomic databases

PRIDEiQ8W1L6.

Expressioni

Gene expression databases

ExpressionAtlasiQ8W1L6. baseline.

Interactioni

Subunit structurei

Monomer.1 PublicationCurated

Protein-protein interaction databases

STRINGi39947.LOC_Os02g17390.1.

Structurei

3D structure databases

ProteinModelPortaliQ8W1L6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The epimerase and isomerase activities are contained in the N-terminal region while the dehydrogenase activity is in the C-terminal region.By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.Curated
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.Curated

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261347.
InParanoidiQ8W1L6.
KOiK10527.
OMAiRICELGR.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8W1L6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGAIRVTME VGADGVAVVT ICNPPVNALH PIIIQGLKEK YAEAMDRDDV
60 70 80 90 100
KAIVLTGAGG KFCGGFDINV FTEVHKTGNV SLMPDVSVEL VSNLMEAGKK
110 120 130 140 150
PSVAAIQGLA LGGGLELTMG CHARISTPEA QLGLPELTLG IIPGFGGTQR
160 170 180 190 200
LPRLVGLPKA IEMMLQSKFI TAKEGKEGGL VDALCSPDEL IKMSRLWALE
210 220 230 240 250
IANYRKPWIR SLARTDRLGS LSEARSVLNS ARQQAKKVAA NLPQHQACLD
260 270 280 290 300
VMEEGVLCGG HAGVLKEAKV FKELVLSPTS KALVHAFFAQ RLTTKVPGVT
310 320 330 340 350
DVQLKPRKIR KVAVIGGGLM GSGIATALLV SNTSVVLKEV NPQFLQRGQK
360 370 380 390 400
MIAANLEGLV KRGSLTKDKM NKAMSLLKGA LDYSDFKDVD MVIEAVIEKI
410 420 430 440 450
PLKQSIFSDL EKVCPPHCIL ATNTSTIDLN VVGEKTNSQD RIIGAHFFSP
460 470 480 490 500
AHIMPLLEIV RTEKTSPQAI LDLITVGKMI KKVPVVVGNC TGFAVNRTFF
510 520 530 540 550
PYTQGSHLLV SIGIDVFRID RVISSFGMPM GPFQLQDLAG YGVALAVKDI
560 570 580 590 600
YAAAFGTRNL DSNLVDLMVQ NGRQGKSNGK GYYLYEKGGK PKPDPSVQVV
610 620 630 640 650
IDEYRRCAKT MPGGKPVTLS DQDILEMIFF PVVNEACRVM DENVVIRASD
660 670 680 690 700
LDIASILGMG FPKFRGGLVF WADTIGAPYI HSKLSKWTEI YGDFFKPSSY
710 720
LEDRAKRSLP LSAPNATQQA SSRSRM
Length:726
Mass (Da):78,461
Last modified:May 30, 2006 - v2
Checksum:iAFB6E3D2AD02E03E
GO

Sequence cautioni

The sequence AAL35606.1 differs from that shown. Reason: Frameshift at positions 544 and 549. Curated
The sequence AAQ13901.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti178 – 1781G → R in AAQ13901. 1 PublicationCurated
Sequence conflicti345 – 3451L → R in AAL35606. (PubMed:11706039)Curated
Sequence conflicti384 – 3841S → C in AAL35606. (PubMed:11706039)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF442962 mRNA. Translation: AAL35606.1. Frameshift.
AP004771 Genomic DNA. Translation: BAD21833.1.
AF220609 mRNA. Translation: AAQ13901.1. Different initiation.
RefSeqiNP_001046536.1. NM_001053071.1.
UniGeneiOs.9729.

Genome annotation databases

EnsemblPlantsiOS02T0274100-01; OS02T0274100-01; OS02G0274100.
GeneIDi4328997.
KEGGiosa:4328997.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF442962 mRNA. Translation: AAL35606.1 . Frameshift.
AP004771 Genomic DNA. Translation: BAD21833.1 .
AF220609 mRNA. Translation: AAQ13901.1 . Different initiation.
RefSeqi NP_001046536.1. NM_001053071.1.
UniGenei Os.9729.

3D structure databases

ProteinModelPortali Q8W1L6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 39947.LOC_Os02g17390.1.

Proteomic databases

PRIDEi Q8W1L6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS02T0274100-01 ; OS02T0274100-01 ; OS02G0274100 .
GeneIDi 4328997.
KEGGi osa:4328997.

Organism-specific databases

Gramenei Q8W1L6.

Phylogenomic databases

eggNOGi COG1250.
HOGENOMi HOG000261347.
InParanoidi Q8W1L6.
KOi K10527.
OMAi RICELGR.

Enzyme and pathway databases

UniPathwayi UPA00659 .

Gene expression databases

ExpressionAtlasi Q8W1L6. baseline.

Family and domain databases

Gene3Di 1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
PROSITEi PS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a rice RNA- and microtubule-binding protein as the multifunctional protein, a peroxisomal enzyme involved in the beta-oxidation of fatty acids."
    Chuong S.D.X., Mullen R.T., Muench D.G.
    J. Biol. Chem. 277:2419-2429(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, RNA-BINDING, MICROTUBULE-BINDING, CATALYTIC ACTIVITY.
    Tissue: Seed1 Publication.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "Isolation and characterization of a multi-functional protein (OsMFP) gene from rice."
    Gaur R., Tyagi A.K.
    Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-726.

Entry informationi

Entry nameiMFP_ORYSJ
AccessioniPrimary (citable) accession number: Q8W1L6
Secondary accession number(s): Q336K1, Q6K7T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: May 30, 2006
Last modified: October 29, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3