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Q8W1L6 (MFP_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peroxisomal fatty acid beta-oxidation multifunctional protein

Short name=MFP

Including the following 2 domains:

  1. Enoyl-CoA hydratase/3-2-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase
    EC=4.2.1.17
    EC=5.1.2.3
    EC=5.3.3.8
  2. 3-hydroxyacyl-CoA dehydrogenase
    EC=1.1.1.35
Gene names
Name:MFP
Ordered Locus Names:Os02g0274100, LOC_Os02g17390
ORF Names:P0413A11.18
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length726 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Multifunctional enzyme involved in fatty acid beta-oxidation. Also binds to RNA and microtubules. Possible role in subcellular mRNA localization and RNA-cytoskeleton interactions. Ref.1

Catalytic activity

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O. Ref.1

(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA. Ref.1

(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA. Ref.1 UniProtKB Q39659

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH. Ref.1

Pathway

Lipid metabolism; fatty acid beta-oxidation.

Subunit structure

Monomer Probable. Ref.1

Subcellular location

Peroxisome Probable. Cytoplasmcytoskeleton Probable.

Domain

The epimerase and isomerase activities are contained in the N-terminal region while the dehydrogenase activity is in the C-terminal region By similarity. UniProtKB Q39659

Sequence similarities

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.

In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Sequence caution

The sequence AAL35606.1 differs from that shown. Reason: Frameshift at positions 544 and 549.

The sequence AAQ13901.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processFatty acid metabolism
Lipid metabolism
   Cellular componentCytoplasm
Cytoskeleton
Microtubule
Peroxisome
   LigandNAD
RNA-binding
   Molecular functionIsomerase
Lyase
Oxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Multifunctional enzyme
Reference proteome
Gene Ontology (GO)
   Biological_processfatty acid beta-oxidation

Inferred from direct assay Ref.1. Source: UniProtKB

flower development

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

jasmonic acid biosynthetic process

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Cellular_componentcell wall

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

microtubule

Inferred from electronic annotation. Source: UniProtKB-KW

peroxisome

Non-traceable author statement Ref.1. Source: UniProtKB

plasmodesma

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_function3-hydroxyacyl-CoA dehydratase activity

Inferred from direct assay Ref.1. Source: UniProtKB

3-hydroxyacyl-CoA dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

3-hydroxybutyryl-CoA epimerase activity

Inferred from sequence or structural similarity PubMed 8051146. Source: UniProtKB

coenzyme binding

Inferred from electronic annotation. Source: InterPro

dodecenoyl-CoA delta-isomerase activity

Inferred from sequence or structural similarity PubMed 8051146. Source: UniProtKB

enoyl-CoA hydratase activity

Inferred from direct assay Ref.1. Source: UniProtKB

mRNA binding

Inferred from direct assay Ref.1. Source: UniProtKB

microtubule binding

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 726726Peroxisomal fatty acid beta-oxidation multifunctional protein
PRO_0000109252

Experimental info

Sequence conflict1781G → R in AAQ13901. Ref.3
Sequence conflict3451L → R in AAL35606. Ref.1
Sequence conflict3841S → C in AAL35606. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8W1L6 [UniParc].

Last modified May 30, 2006. Version 2.
Checksum: AFB6E3D2AD02E03E

FASTA72678,461
        10         20         30         40         50         60 
MAGAIRVTME VGADGVAVVT ICNPPVNALH PIIIQGLKEK YAEAMDRDDV KAIVLTGAGG 

        70         80         90        100        110        120 
KFCGGFDINV FTEVHKTGNV SLMPDVSVEL VSNLMEAGKK PSVAAIQGLA LGGGLELTMG 

       130        140        150        160        170        180 
CHARISTPEA QLGLPELTLG IIPGFGGTQR LPRLVGLPKA IEMMLQSKFI TAKEGKEGGL 

       190        200        210        220        230        240 
VDALCSPDEL IKMSRLWALE IANYRKPWIR SLARTDRLGS LSEARSVLNS ARQQAKKVAA 

       250        260        270        280        290        300 
NLPQHQACLD VMEEGVLCGG HAGVLKEAKV FKELVLSPTS KALVHAFFAQ RLTTKVPGVT 

       310        320        330        340        350        360 
DVQLKPRKIR KVAVIGGGLM GSGIATALLV SNTSVVLKEV NPQFLQRGQK MIAANLEGLV 

       370        380        390        400        410        420 
KRGSLTKDKM NKAMSLLKGA LDYSDFKDVD MVIEAVIEKI PLKQSIFSDL EKVCPPHCIL 

       430        440        450        460        470        480 
ATNTSTIDLN VVGEKTNSQD RIIGAHFFSP AHIMPLLEIV RTEKTSPQAI LDLITVGKMI 

       490        500        510        520        530        540 
KKVPVVVGNC TGFAVNRTFF PYTQGSHLLV SIGIDVFRID RVISSFGMPM GPFQLQDLAG 

       550        560        570        580        590        600 
YGVALAVKDI YAAAFGTRNL DSNLVDLMVQ NGRQGKSNGK GYYLYEKGGK PKPDPSVQVV 

       610        620        630        640        650        660 
IDEYRRCAKT MPGGKPVTLS DQDILEMIFF PVVNEACRVM DENVVIRASD LDIASILGMG 

       670        680        690        700        710        720 
FPKFRGGLVF WADTIGAPYI HSKLSKWTEI YGDFFKPSSY LEDRAKRSLP LSAPNATQQA 


SSRSRM 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a rice RNA- and microtubule-binding protein as the multifunctional protein, a peroxisomal enzyme involved in the beta-oxidation of fatty acids."
Chuong S.D.X., Mullen R.T., Muench D.G.
J. Biol. Chem. 277:2419-2429(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, RNA-BINDING, MICROTUBULE-BINDING, CATALYTIC ACTIVITY.
Tissue: Seed.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"Isolation and characterization of a multi-functional protein (OsMFP) gene from rice."
Gaur R., Tyagi A.K.
Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-726.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF442962 mRNA. Translation: AAL35606.1. Frameshift.
AP004771 Genomic DNA. Translation: BAD21833.1.
AF220609 mRNA. Translation: AAQ13901.1. Different initiation.
RefSeqNP_001046536.1. NM_001053071.1.
UniGeneOs.9729.

3D structure databases

ProteinModelPortalQ8W1L6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39947.LOC_Os02g17390.1.

Proteomic databases

PRIDEQ8W1L6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS02T0274100-01; OS02T0274100-01; OS02G0274100.
GeneID4328997.
KEGGosa:4328997.

Organism-specific databases

GrameneQ8W1L6.

Phylogenomic databases

eggNOGCOG1250.
HOGENOMHOG000261347.
KOK10527.
OMARICELGR.

Enzyme and pathway databases

UniPathwayUPA00659.

Family and domain databases

Gene3D1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
InterProIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
PROSITEPS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMFP_ORYSJ
AccessionPrimary (citable) accession number: Q8W1L6
Secondary accession number(s): Q336K1, Q6K7T9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: May 30, 2006
Last modified: June 11, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways