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Q8W1L6

- MFP_ORYSJ

UniProt

Q8W1L6 - MFP_ORYSJ

Protein

Peroxisomal fatty acid beta-oxidation multifunctional protein

Gene

MFP

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 2 (30 May 2006)
      Previous versions | rss
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    Functioni

    Multifunctional enzyme involved in fatty acid beta-oxidation. Also binds to RNA and microtubules. Possible role in subcellular mRNA localization and RNA-cytoskeleton interactions.1 Publication1 Publication

    Catalytic activityi

    (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.1 Publication
    (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.1 Publication
    (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.By similarity1 Publication
    (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. 3-hydroxyacyl-CoA dehydratase activity Source: UniProtKB
    2. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-EC
    3. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB
    4. coenzyme binding Source: InterPro
    5. dodecenoyl-CoA delta-isomerase activity Source: UniProtKB
    6. enoyl-CoA hydratase activity Source: UniProtKB
    7. microtubule binding Source: UniProtKB
    8. mRNA binding Source: UniProtKB

    GO - Biological processi

    1. fatty acid beta-oxidation Source: UniProtKB
    2. flower development Source: EnsemblPlants/Gramene
    3. jasmonic acid biosynthetic process Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Isomerase, Lyase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    NAD, RNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisomal fatty acid beta-oxidation multifunctional protein
    Short name:
    MFP
    Including the following 2 domains:
    Enoyl-CoA hydratase/3-2-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3, EC:5.3.3.8)
    3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
    Gene namesi
    Name:MFP
    Ordered Locus Names:Os02g0274100, LOC_Os02g17390
    ORF Names:P0413A11.18
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 2

    Organism-specific databases

    GrameneiQ8W1L6.

    Subcellular locationi

    Peroxisome Curated. Cytoplasmcytoskeleton Curated

    GO - Cellular componenti

    1. cell wall Source: EnsemblPlants/Gramene
    2. chloroplast Source: EnsemblPlants/Gramene
    3. microtubule Source: UniProtKB-KW
    4. peroxisome Source: UniProtKB
    5. plasmodesma Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 726726Peroxisomal fatty acid beta-oxidation multifunctional proteinPRO_0000109252Add
    BLAST

    Proteomic databases

    PRIDEiQ8W1L6.

    Interactioni

    Subunit structurei

    Monomer.1 PublicationCurated

    Protein-protein interaction databases

    STRINGi39947.LOC_Os02g17390.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8W1L6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The epimerase and isomerase activities are contained in the N-terminal region while the dehydrogenase activity is in the C-terminal region.By similarity

    Sequence similaritiesi

    In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.Curated
    In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.Curated

    Phylogenomic databases

    eggNOGiCOG1250.
    HOGENOMiHOG000261347.
    KOiK10527.
    OMAiRICELGR.

    Family and domain databases

    Gene3Di1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR018376. Enoyl-CoA_hyd/isom_CS.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00725. 3HCDH. 1 hit.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    PROSITEiPS00067. 3HCDH. 1 hit.
    PS00166. ENOYL_COA_HYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8W1L6-1 [UniParc]FASTAAdd to Basket

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    MAGAIRVTME VGADGVAVVT ICNPPVNALH PIIIQGLKEK YAEAMDRDDV    50
    KAIVLTGAGG KFCGGFDINV FTEVHKTGNV SLMPDVSVEL VSNLMEAGKK 100
    PSVAAIQGLA LGGGLELTMG CHARISTPEA QLGLPELTLG IIPGFGGTQR 150
    LPRLVGLPKA IEMMLQSKFI TAKEGKEGGL VDALCSPDEL IKMSRLWALE 200
    IANYRKPWIR SLARTDRLGS LSEARSVLNS ARQQAKKVAA NLPQHQACLD 250
    VMEEGVLCGG HAGVLKEAKV FKELVLSPTS KALVHAFFAQ RLTTKVPGVT 300
    DVQLKPRKIR KVAVIGGGLM GSGIATALLV SNTSVVLKEV NPQFLQRGQK 350
    MIAANLEGLV KRGSLTKDKM NKAMSLLKGA LDYSDFKDVD MVIEAVIEKI 400
    PLKQSIFSDL EKVCPPHCIL ATNTSTIDLN VVGEKTNSQD RIIGAHFFSP 450
    AHIMPLLEIV RTEKTSPQAI LDLITVGKMI KKVPVVVGNC TGFAVNRTFF 500
    PYTQGSHLLV SIGIDVFRID RVISSFGMPM GPFQLQDLAG YGVALAVKDI 550
    YAAAFGTRNL DSNLVDLMVQ NGRQGKSNGK GYYLYEKGGK PKPDPSVQVV 600
    IDEYRRCAKT MPGGKPVTLS DQDILEMIFF PVVNEACRVM DENVVIRASD 650
    LDIASILGMG FPKFRGGLVF WADTIGAPYI HSKLSKWTEI YGDFFKPSSY 700
    LEDRAKRSLP LSAPNATQQA SSRSRM 726
    Length:726
    Mass (Da):78,461
    Last modified:May 30, 2006 - v2
    Checksum:iAFB6E3D2AD02E03E
    GO

    Sequence cautioni

    The sequence AAL35606.1 differs from that shown. Reason: Frameshift at positions 544 and 549.
    The sequence AAQ13901.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti178 – 1781G → R in AAQ13901. 1 PublicationCurated
    Sequence conflicti345 – 3451L → R in AAL35606. (PubMed:11706039)Curated
    Sequence conflicti384 – 3841S → C in AAL35606. (PubMed:11706039)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF442962 mRNA. Translation: AAL35606.1. Frameshift.
    AP004771 Genomic DNA. Translation: BAD21833.1.
    AF220609 mRNA. Translation: AAQ13901.1. Different initiation.
    RefSeqiNP_001046536.1. NM_001053071.1.
    UniGeneiOs.9729.

    Genome annotation databases

    EnsemblPlantsiOS02T0274100-01; OS02T0274100-01; OS02G0274100.
    GeneIDi4328997.
    KEGGiosa:4328997.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF442962 mRNA. Translation: AAL35606.1 . Frameshift.
    AP004771 Genomic DNA. Translation: BAD21833.1 .
    AF220609 mRNA. Translation: AAQ13901.1 . Different initiation.
    RefSeqi NP_001046536.1. NM_001053071.1.
    UniGenei Os.9729.

    3D structure databases

    ProteinModelPortali Q8W1L6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 39947.LOC_Os02g17390.1.

    Proteomic databases

    PRIDEi Q8W1L6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS02T0274100-01 ; OS02T0274100-01 ; OS02G0274100 .
    GeneIDi 4328997.
    KEGGi osa:4328997.

    Organism-specific databases

    Gramenei Q8W1L6.

    Phylogenomic databases

    eggNOGi COG1250.
    HOGENOMi HOG000261347.
    KOi K10527.
    OMAi RICELGR.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .

    Family and domain databases

    Gene3Di 1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR018376. Enoyl-CoA_hyd/isom_CS.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00725. 3HCDH. 1 hit.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    PROSITEi PS00067. 3HCDH. 1 hit.
    PS00166. ENOYL_COA_HYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a rice RNA- and microtubule-binding protein as the multifunctional protein, a peroxisomal enzyme involved in the beta-oxidation of fatty acids."
      Chuong S.D.X., Mullen R.T., Muench D.G.
      J. Biol. Chem. 277:2419-2429(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, RNA-BINDING, MICROTUBULE-BINDING, CATALYTIC ACTIVITY.
      Tissue: Seed1 Publication.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "Isolation and characterization of a multi-functional protein (OsMFP) gene from rice."
      Gaur R., Tyagi A.K.
      Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-726.

    Entry informationi

    Entry nameiMFP_ORYSJ
    AccessioniPrimary (citable) accession number: Q8W1L6
    Secondary accession number(s): Q336K1, Q6K7T9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2003
    Last sequence update: May 30, 2006
    Last modified: October 1, 2014
    This is version 83 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3