Peroxisomal fatty acid beta-oxidation multifunctional protein

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Q8W1L6 (MFP_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 75. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Peroxisomal fatty acid beta-oxidation multifunctional protein
Short name=MFP

Including the following 2 domains:

Enoyl-CoA hydratase/3-2-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase
EC=4.2.1.17
EC=5.1.2.3
EC=5.3.3.8
3-hydroxyacyl-CoA dehydrogenase
EC=1.1.1.35

Gene names

Name:	MFP
Ordered Locus Names:	Os02g0274100, LOC_Os02g17390
ORF Names:	P0413A11.18

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza ›

Protein attributes

Sequence length	726 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Multifunctional enzyme involved in fatty acid beta-oxidation. Also binds to RNA and microtubules. Possible role in subcellular mRNA localization and RNA-cytoskeleton interactions. Ref.1
Catalytic activity	(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H₂O. Ref.1 (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA. Ref.1 (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA. Ref.1 UniProtKB Q39659 (S)-3-hydroxyacyl-CoA + NAD⁺ = 3-oxoacyl-CoA + NADH. Ref.1
Pathway	Lipid metabolism; fatty acid beta-oxidation.
Subunit structure	Monomer Probable. Ref.1
Subcellular location	Peroxisome Probable. Cytoplasm › cytoskeleton Probable.
Domain	The epimerase and isomerase activities are contained in the N-terminal region while the dehydrogenase activity is in the C-terminal region By similarity. UniProtKB Q39659
Sequence similarities	In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
Sequence caution	The sequence AAL35606.1 differs from that shown. Reason: Frameshift at positions 544 and 549. The sequence AAQ13901.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Cytoplasm Cytoskeleton Microtubule Peroxisome
Ligand	NAD RNA-binding
Molecular function	Isomerase Lyase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid beta-oxidation Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	microtubule Inferred from electronic annotation. Source: UniProtKB-KW peroxisome Non-traceable author statement Ref.1. Source: UniProtKB
Molecular_function	3-hydroxyacyl-CoA dehydratase activity Inferred from direct assay Ref.1. Source: UniProtKB 3-hydroxyacyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: EC 3-hydroxybutyryl-CoA epimerase activity Inferred from sequence or structural similarity PubMed 8051146. Source: UniProtKB coenzyme binding Inferred from electronic annotation. Source: InterPro dodecenoyl-CoA delta-isomerase activity Inferred from sequence or structural similarity PubMed 8051146. Source: UniProtKB enoyl-CoA hydratase activity Inferred from direct assay Ref.1. Source: UniProtKB mRNA binding Inferred from direct assay Ref.1. Source: UniProtKB microtubule binding Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 726

726

Peroxisomal fatty acid beta-oxidation multifunctional protein

PRO_0000109252

Experimental info

Sequence conflict

178

G → R in AAQ13901. Ref.3

Sequence conflict

345

L → R in AAL35606. Ref.1

Sequence conflict

384

S → C in AAL35606. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q8W1L6 [UniParc].

Last modified May 30, 2006. Version 2.
Checksum: AFB6E3D2AD02E03E
Show »

FASTA

726

78,461

        10         20         30         40         50         60 
MAGAIRVTME VGADGVAVVT ICNPPVNALH PIIIQGLKEK YAEAMDRDDV KAIVLTGAGG 

        70         80         90        100        110        120 
KFCGGFDINV FTEVHKTGNV SLMPDVSVEL VSNLMEAGKK PSVAAIQGLA LGGGLELTMG 

       130        140        150        160        170        180 
CHARISTPEA QLGLPELTLG IIPGFGGTQR LPRLVGLPKA IEMMLQSKFI TAKEGKEGGL 

       190        200        210        220        230        240 
VDALCSPDEL IKMSRLWALE IANYRKPWIR SLARTDRLGS LSEARSVLNS ARQQAKKVAA 

       250        260        270        280        290        300 
NLPQHQACLD VMEEGVLCGG HAGVLKEAKV FKELVLSPTS KALVHAFFAQ RLTTKVPGVT 

       310        320        330        340        350        360 
DVQLKPRKIR KVAVIGGGLM GSGIATALLV SNTSVVLKEV NPQFLQRGQK MIAANLEGLV 

       370        380        390        400        410        420 
KRGSLTKDKM NKAMSLLKGA LDYSDFKDVD MVIEAVIEKI PLKQSIFSDL EKVCPPHCIL 

       430        440        450        460        470        480 
ATNTSTIDLN VVGEKTNSQD RIIGAHFFSP AHIMPLLEIV RTEKTSPQAI LDLITVGKMI 

       490        500        510        520        530        540 
KKVPVVVGNC TGFAVNRTFF PYTQGSHLLV SIGIDVFRID RVISSFGMPM GPFQLQDLAG 

       550        560        570        580        590        600 
YGVALAVKDI YAAAFGTRNL DSNLVDLMVQ NGRQGKSNGK GYYLYEKGGK PKPDPSVQVV 

       610        620        630        640        650        660 
IDEYRRCAKT MPGGKPVTLS DQDILEMIFF PVVNEACRVM DENVVIRASD LDIASILGMG 

       670        680        690        700        710        720 
FPKFRGGLVF WADTIGAPYI HSKLSKWTEI YGDFFKPSSY LEDRAKRSLP LSAPNATQQA 


SSRSRM

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a rice RNA- and microtubule-binding protein as the multifunctional protein, a peroxisomal enzyme involved in the beta-oxidation of fatty acids." Chuong S.D.X., Mullen R.T., Muench D.G. J. Biol. Chem. 277:2419-2429(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, RNA-BINDING, MICROTUBULE-BINDING, CATALYTIC ACTIVITY. Tissue: Seed.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Isolation and characterization of a multi-functional protein (OsMFP) gene from rice." Gaur R., Tyagi A.K. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-726.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF442962 mRNA. Translation: AAL35606.1. Frameshift. AP004771 Genomic DNA. Translation: BAD21833.1. AF220609 mRNA. Translation: AAQ13901.1. Different initiation.
RefSeq	NP_001046536.1. NM_001053071.1.
UniGene	Os.9729.
3D structure databases
ProteinModelPortal	Q8W1L6.
ModBase	Search...
Protein-protein interaction databases
STRING	39947.LOC_Os02g17390.1.
Proteomic databases
PRIDE	Q8W1L6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os02g17390.1; LOC_Os02g17390.1; LOC_Os02g17390.
GeneID	4328997.
KEGG	dosa:Os02t0274100-01. osa:4328997.
Organism-specific databases
Gramene	Q8W1L6.
Phylogenomic databases
eggNOG	COG1250.
HOGENOM	HOG000261347.
KO	K10527.
OMA	DIDIVWI.
ProtClustDB	CLSN2916032.
Enzyme and pathway databases
UniPathway	UPA00659.
Gene expression databases
ArrayExpress	Q8W1L6.
Family and domain databases
Gene3D	1.10.1040.10. 2 hits. 3.40.50.720. 1 hit.
InterPro	IPR006180. 3-OHacyl-CoA_DH_CS. IPR006176. 3-OHacyl-CoA_DH_NAD-bd. IPR006108. 3HC_DH_C. IPR008927. 6-PGluconate_DH_C-like. IPR001753. Crotonase_core_superfam. IPR013328. DH_multihelical. IPR018376. Enoyl-CoA_hyd/isom_CS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF00725. 3HCDH. 1 hit. PF02737. 3HCDH_N. 1 hit. PF00378. ECH. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 2 hits.
PROSITE	PS00067. 3HCDH. 1 hit. PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MFP_ORYSJ

Accession

Primary (citable) accession number: Q8W1L6
Secondary accession number(s): Q336K1, Q6K7T9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	May 30, 2006
Last modified:	May 29, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Including the following 2 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents