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Protein

Peroxisomal fatty acid beta-oxidation multifunctional protein

Gene

MFP

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Multifunctional enzyme involved in fatty acid beta-oxidation. Also binds to RNA and microtubules. Possible role in subcellular mRNA localization and RNA-cytoskeleton interactions.1 Publication1 Publication

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.1 Publication
A (3Z)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.1 Publication
A (3E)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.1 Publication
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.By similarity1 Publication
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.1 Publication

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

GO - Molecular functioni

  • 3-hydroxyacyl-CoA dehydratase activity Source: UniProtKB
  • 3-hydroxyacyl-CoA dehydrogenase activity Source: GO_Central
  • 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB
  • dodecenoyl-CoA delta-isomerase activity Source: UniProtKB
  • enoyl-CoA hydratase activity Source: UniProtKB
  • microtubule binding Source: UniProtKB
  • mRNA binding Source: UniProtKB

GO - Biological processi

  • fatty acid beta-oxidation Source: UniProtKB

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme, Oxidoreductase, RNA-binding
Biological processFatty acid metabolism, Lipid metabolism
LigandNAD

Enzyme and pathway databases

ReactomeiR-OSA-390247 Beta-oxidation of very long chain fatty acids
UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal fatty acid beta-oxidation multifunctional protein
Short name:
MFP
Including the following 2 domains:
Enoyl-CoA hydratase/3-2-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3, EC:5.3.3.8)
3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
Gene namesi
Name:MFP
Ordered Locus Names:Os02g0274100, LOC_Os02g17390
ORF Names:P0413A11.18
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001092521 – 726Peroxisomal fatty acid beta-oxidation multifunctional proteinAdd BLAST726

Proteomic databases

PaxDbiQ8W1L6
PRIDEiQ8W1L6

Expressioni

Gene expression databases

ExpressionAtlasiQ8W1L6 differential
GenevisibleiQ8W1L6 OS

Interactioni

Subunit structurei

Monomer.1 PublicationCurated

GO - Molecular functioni

  • microtubule binding Source: UniProtKB

Protein-protein interaction databases

STRINGi39947.LOC_Os02g17390.1

Structurei

3D structure databases

ProteinModelPortaliQ8W1L6
SMRiQ8W1L6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The epimerase and isomerase activities are contained in the N-terminal region while the dehydrogenase activity is in the C-terminal region.By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.Curated
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.Curated

Phylogenomic databases

eggNOGiKOG1683 Eukaryota
COG1024 LUCA
COG1250 LUCA
HOGENOMiHOG000261347
InParanoidiQ8W1L6
KOiK10527
OMAiIDLCMIL
OrthoDBiEOG09360359

Family and domain databases

InterProiView protein in InterPro
IPR006180 3-OHacyl-CoA_DH_CS
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR033346 AIM1
IPR029045 ClpP/crotonase-like_dom_sf
IPR018376 Enoyl-CoA_hyd/isom_CS
IPR001753 Enoyl-CoA_hydra/iso
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR23309:SF10 PTHR23309:SF10, 1 hit
PfamiView protein in Pfam
PF00725 3HCDH, 1 hit
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
PROSITEiView protein in PROSITE
PS00067 3HCDH, 1 hit
PS00166 ENOYL_COA_HYDRATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8W1L6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGAIRVTME VGADGVAVVT ICNPPVNALH PIIIQGLKEK YAEAMDRDDV
60 70 80 90 100
KAIVLTGAGG KFCGGFDINV FTEVHKTGNV SLMPDVSVEL VSNLMEAGKK
110 120 130 140 150
PSVAAIQGLA LGGGLELTMG CHARISTPEA QLGLPELTLG IIPGFGGTQR
160 170 180 190 200
LPRLVGLPKA IEMMLQSKFI TAKEGKEGGL VDALCSPDEL IKMSRLWALE
210 220 230 240 250
IANYRKPWIR SLARTDRLGS LSEARSVLNS ARQQAKKVAA NLPQHQACLD
260 270 280 290 300
VMEEGVLCGG HAGVLKEAKV FKELVLSPTS KALVHAFFAQ RLTTKVPGVT
310 320 330 340 350
DVQLKPRKIR KVAVIGGGLM GSGIATALLV SNTSVVLKEV NPQFLQRGQK
360 370 380 390 400
MIAANLEGLV KRGSLTKDKM NKAMSLLKGA LDYSDFKDVD MVIEAVIEKI
410 420 430 440 450
PLKQSIFSDL EKVCPPHCIL ATNTSTIDLN VVGEKTNSQD RIIGAHFFSP
460 470 480 490 500
AHIMPLLEIV RTEKTSPQAI LDLITVGKMI KKVPVVVGNC TGFAVNRTFF
510 520 530 540 550
PYTQGSHLLV SIGIDVFRID RVISSFGMPM GPFQLQDLAG YGVALAVKDI
560 570 580 590 600
YAAAFGTRNL DSNLVDLMVQ NGRQGKSNGK GYYLYEKGGK PKPDPSVQVV
610 620 630 640 650
IDEYRRCAKT MPGGKPVTLS DQDILEMIFF PVVNEACRVM DENVVIRASD
660 670 680 690 700
LDIASILGMG FPKFRGGLVF WADTIGAPYI HSKLSKWTEI YGDFFKPSSY
710 720
LEDRAKRSLP LSAPNATQQA SSRSRM
Length:726
Mass (Da):78,461
Last modified:May 30, 2006 - v2
Checksum:iAFB6E3D2AD02E03E
GO

Sequence cautioni

The sequence AAL35606 differs from that shown. Reason: Frameshift at positions 544 and 549.Curated
The sequence AAQ13901 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti178G → R in AAQ13901 (Ref. 5) Curated1
Sequence conflicti345L → R in AAL35606 (PubMed:11706039).Curated1
Sequence conflicti384S → C in AAL35606 (PubMed:11706039).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF442962 mRNA Translation: AAL35606.1 Frameshift.
AP004771 Genomic DNA Translation: BAD21833.1
AP008208 Genomic DNA Translation: BAF08450.1
AP014958 Genomic DNA Translation: BAS78079.1
AF220609 mRNA Translation: AAQ13901.1 Different initiation.
RefSeqiXP_015625624.1, XM_015770138.1
UniGeneiOs.9729

Genome annotation databases

EnsemblPlantsiOs02t0274100-01; Os02t0274100-01; Os02g0274100
GeneIDi4328997
GrameneiOs02t0274100-01; Os02t0274100-01; Os02g0274100
KEGGiosa:4328997

Similar proteinsi

Entry informationi

Entry nameiMFP_ORYSJ
AccessioniPrimary (citable) accession number: Q8W1L6
Secondary accession number(s): Q0E238, Q336K1, Q6K7T9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: May 30, 2006
Last modified: May 23, 2018
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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