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Protein

Cyclic dof factor 1

Gene

CDF1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence. A flanking TGT sequence contributes to the specificity of binding. Regulates a photoperiodic flowering response. Transcriptional repressor of 'CONSTANS' expression. The DNA-binding ability is not modulated by 'GIGANTEA' but the stability of CDF1 is controlled by the proteasome-dependent pathway. Ubiquitinated by the SCF(ADO3) E3 ubiquitin ligase complex. Binds to the FT promoter in the morning.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri54 – 10855Dof-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • chloroplast organization Source: TAIR
  • flower development Source: UniProtKB-KW
  • negative regulation of transcription, DNA-templated Source: TAIR
  • regulation of timing of transition from vegetative to reproductive phase Source: TAIR
  • regulation of transcription, DNA-templated Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Flowering, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic dof factor 1
Alternative name(s):
Dof zinc finger protein DOF5.5
Short name:
AtDOF5.5
Gene namesi
Name:CDF1
Synonyms:DOF5.5
Ordered Locus Names:At5g62430
ORF Names:K19B1.4, MMI9.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G62430.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi253 – 2531K → A: Reduced binding to ADO3 and increased stability. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Cyclic dof factor 1PRO_0000074295Add
BLAST

Post-translational modificationi

Ubiquitinated.Curated

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ8W1E3.
PRIDEiQ8W1E3.

Expressioni

Tissue specificityi

Expressed in the vascular tissues of cotyledons, leaves and hypocotyls and in stomata. Not detected in roots.1 Publication

Inductioni

Circadian-regulation at the protein level, but not at the mRNA level. Strongly decreased expression during the dark phase. Accumulates at high levels at the beginning of the day.1 Publication

Gene expression databases

GenevisibleiQ8W1E3. AT.

Interactioni

Subunit structurei

Interacts with ADO2 (via kelch repeats), ADO3 (via kelch repeats) and GI (via N-terminus).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ADO2Q8W4202EBI-1536051,EBI-1015688
ADO3Q9C9W92EBI-1536051,EBI-401228
GIQ9SQI23EBI-1536051,EBI-446380

Protein-protein interaction databases

BioGridi21608. 5 interactions.
IntActiQ8W1E3. 5 interactions.
STRINGi3702.AT5G62430.1.

Family & Domainsi

Sequence similaritiesi

Contains 1 Dof-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri54 – 10855Dof-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410JC2Q. Eukaryota.
ENOG410ZK6N. LUCA.
HOGENOMiHOG000239772.
KOiK16222.
OMAiQHATISE.
OrthoDBiEOG0936092V.
PhylomeDBiQ8W1E3.

Family and domain databases

InterProiIPR003851. Znf_Dof.
[Graphical view]
PfamiPF02701. zf-Dof. 1 hit.
[Graphical view]
ProDomiPD007478. Znf_Dof. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS01361. ZF_DOF_1. 1 hit.
PS50884. ZF_DOF_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8W1E3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLETKDPAIK LFGMKIPFPT VLEVADEEEE KNQNKTLTDQ SEKDKTLKKP
60 70 80 90 100
TKILPCPRCN SMETKFCYYN NYNVNQPRHF CKACQRYWTS GGTMRSVPIG
110 120 130 140 150
AGRRKNKNNS PTSHYHHVTI SETNGPVLSF SLGDDQKVSS NRFGNQKLVA
160 170 180 190 200
RIENNDERSN NNTSNGLNCF PGVSWPYTWN PAFYPVYPYW SMPVLSSPVS
210 220 230 240 250
SSPTSTLGKH SRDEDETVKQ KQRNGSVLVP KTLRIDDPNE AAKSSIWTTL
260 270 280 290
GIKNEVMFNG FGSKKEVKLS NKEETETSLV LCANPAALSR SINFHEQM
Note: Gene prediction confirmed by EST data. No experimental confirmation available.
Length:298
Mass (Da):33,639
Last modified:November 9, 2004 - v2
Checksum:i683CEB24D4F22D75
GO
Isoform 2 (identifier: Q8W1E3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.

Note: No experimental confirmation available.
Show »
Length:237
Mass (Da):26,696
Checksum:iF35D4F1ADB11E0A6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti84 – 841C → Y in AAL48235 (PubMed:14593172).Curated
Sequence conflicti84 – 841C → Y in AAN28769 (PubMed:14593172).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6161Missing in isoform 2. 1 PublicationVSP_011870Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB231333 mRNA. Translation: BAE16983.1.
AB015469 Genomic DNA. Translation: BAB11493.1.
CP002688 Genomic DNA. Translation: AED97607.1.
AF446362 mRNA. Translation: AAL48235.1.
AY143830 mRNA. Translation: AAN28769.1.
RefSeqiNP_201049.3. NM_125637.3. [Q8W1E3-1]
UniGeneiAt.9300.

Genome annotation databases

EnsemblPlantsiAT5G62430.1; AT5G62430.1; AT5G62430. [Q8W1E3-1]
GeneIDi836364.
KEGGiath:AT5G62430.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB231333 mRNA. Translation: BAE16983.1.
AB015469 Genomic DNA. Translation: BAB11493.1.
CP002688 Genomic DNA. Translation: AED97607.1.
AF446362 mRNA. Translation: AAL48235.1.
AY143830 mRNA. Translation: AAN28769.1.
RefSeqiNP_201049.3. NM_125637.3. [Q8W1E3-1]
UniGeneiAt.9300.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21608. 5 interactions.
IntActiQ8W1E3. 5 interactions.
STRINGi3702.AT5G62430.1.

Proteomic databases

PaxDbiQ8W1E3.
PRIDEiQ8W1E3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G62430.1; AT5G62430.1; AT5G62430. [Q8W1E3-1]
GeneIDi836364.
KEGGiath:AT5G62430.

Organism-specific databases

TAIRiAT5G62430.

Phylogenomic databases

eggNOGiENOG410JC2Q. Eukaryota.
ENOG410ZK6N. LUCA.
HOGENOMiHOG000239772.
KOiK16222.
OMAiQHATISE.
OrthoDBiEOG0936092V.
PhylomeDBiQ8W1E3.

Miscellaneous databases

PROiQ8W1E3.

Gene expression databases

GenevisibleiQ8W1E3. AT.

Family and domain databases

InterProiIPR003851. Znf_Dof.
[Graphical view]
PfamiPF02701. zf-Dof. 1 hit.
[Graphical view]
ProDomiPD007478. Znf_Dof. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS01361. ZF_DOF_1. 1 hit.
PS50884. ZF_DOF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDF1_ARATH
AccessioniPrimary (citable) accession number: Q8W1E3
Secondary accession number(s): Q4ACT9, Q9FJJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: November 9, 2004
Last modified: September 7, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.