Reviewed, UniProtKB/Swiss-Prot Q8W1E3 (DOF55_ARATH)

Names and origin

Protein names

Recommended name:
    Dof zinc finger protein DOF5.5
      Short name=AtDOF5.5

Gene names

Name:	DOF5.5
Ordered Locus Names:	At5g62430
ORF Names:	K19B1.4, MMI9.24

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence By similarity.
Subcellular location	Nucleus Probable.
Sequence similarities	Contains 1 Dof-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	negative regulation of transcription Inferred from direct assay. Source: TAIR regulation of timing of transition from vegetative to reproductive phase Inferred from mutant phenotype. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from direct assay. Source: TAIR
Molecular function	DNA binding Inferred from direct assay. Source: TAIR protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ADO2	Q8W420	1	EBI-1536051,EBI-1015688
ADO3	Q9C9W9	1	EBI-1536051,EBI-401228
GI	Q9SQI2	2	EBI-1536051,EBI-446380

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8W1E3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Gene prediction confirmed by EST data. No experimental confirmation available.

Isoform 2 (identifier: Q8W1E3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Dof zinc finger protein DOF5.5		PRO_0000074295
Regions
	Zinc finger	54 – 108	55	Dof-type
Natural variations
	Alternative sequence	1 – 61	61	Missing in isoform 2.		VSP_011870
Experimental info
	Sequence conflict	84	1	C → Y in AAL48235. Ref.2
	Sequence conflict	84	1	C → Y in AAN28769. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 9, 2004. Version 2. Checksum: 683CEB24D4F22D75	FASTA	298	33,639
	10 20 30 40 50 60 MLETKDPAIK LFGMKIPFPT VLEVADEEEE KNQNKTLTDQ SEKDKTLKKP TKILPCPRCN 70 80 90 100 110 120 SMETKFCYYN NYNVNQPRHF CKACQRYWTS GGTMRSVPIG AGRRKNKNNS PTSHYHHVTI 130 140 150 160 170 180 SETNGPVLSF SLGDDQKVSS NRFGNQKLVA RIENNDERSN NNTSNGLNCF PGVSWPYTWN 190 200 210 220 230 240 PAFYPVYPYW SMPVLSSPVS SSPTSTLGKH SRDEDETVKQ KQRNGSVLVP KTLRIDDPNE 250 260 270 280 290 AAKSSIWTTL GIKNEVMFNG FGSKKEVKLS NKEETETSLV LCANPAALSR SINFHEQM
	Isoform 2. Checksum: F35D4F1ADB11E0A6	FASTA	237	26,696
	10 20 30 40 50 60 METKFCYYNN YNVNQPRHFC KACQRYWTSG GTMRSVPIGA GRRKNKNNSP TSHYHHVTIS 70 80 90 100 110 120 ETNGPVLSFS LGDDQKVSSN RFGNQKLVAR IENNDERSNN NTSNGLNCFP GVSWPYTWNP 130 140 150 160 170 180 AFYPVYPYWS MPVLSSPVSS SPTSTLGKHS RDEDETVKQK QRNGSVLVPK TLRIDDPNEA 190 200 210 220 230 AKSSIWTTLG IKNEVMFNGF GSKKEVKLSN KEETETSLVL CANPAALSRS INFHEQM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones." Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S. DNA Res. 5:297-308(1998) [PubMed: 9872454] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[3]	"The Dof family of plant transcription factors." Yanagisawa S. Trends Plant Sci. 7:555-560(2002) [PubMed: 12475498] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB015469 Genomic DNA. Translation: BAB11493.1. AF446362 mRNA. Translation: AAL48235.1. AY143830 mRNA. Translation: AAN28769.1.
IPI	IPI00525269. IPI00529068.
RefSeq	NP_201049.3.
UniGene	At.9300
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8W1E3. 5 interactions.
Genome annotation databases
GeneID	836364.
GenomeReviews	Gene locus AT5G62430 in contig BA000015_GR.
KEGG	ath:AT5G62430.
NMPDR	fig|3702.1.peg.28280.
Organism-specific databases
GeneFarm	4477. 446.
TAIR	At5g62430.
Phylogenomic databases
OMA	Q8W1E3. ARIENND.
Gene expression databases
ArrayExpress	Q8W1E3.
GermOnline	AT5G62430. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003851. Znf_Dof. [Graphical view]
Pfam	PF02701. zf-Dof. 1 hit. [Graphical view]
PROSITE	PS01361. ZF_DOF_1. 1 hit. PS50884. ZF_DOF_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DOF55_ARATH

Accession

Primary (citable) accession number: Q8W1E3
Secondary accession number(s): Q9FJJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 9, 2004
Last sequence update:	November 9, 2004
Last modified:	June 16, 2009
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)