Q8W0A1 (BGAL2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase 2
Short name=Lactase 2
EC=3.2.1.23

Gene names

Ordered Locus Names:	Os01g0580200, LOC_Os01g39830
ORF Names:	P0672C09.21

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	827 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family. Contains 1 SUEL-type lectin domain.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 827	803	Beta-galactosidase 2		PRO_0000294153
Regions
	Domain	741 – 827	87	SUEL-type lectin
Sites
	Active site	182	1	Proton donor Potential
	Active site	251	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	209	1	N-linked (GlcNAc...) Potential
	Glycosylation	458	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8W0A1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 100D0225FF558261	FASTA	827	91,549
	10 20 30 40 50 60 MAASAVAVAF VVAVAAVLAA AASAAVTYDR KAVVVNGQRR ILISGSIHYP RSTPEMWPDL 70 80 90 100 110 120 IEKAKDGGLD VVQTYVFWNG HEPSPGQYYF EGRYDLVHFI KLVKQAGLYV NLRIGPYVCA 130 140 150 160 170 180 EWNFGGFPVW LKYVPGISFR TDNEPFKAEM QKFTTKIVEM MKSEGLFEWQ GGPIILSQIE 190 200 210 220 230 240 NEFGPLEWDQ GEPAKAYASW AANMAVALNT SVPWIMCKED DAPDPIINTC NGFYCDWFSP 250 260 270 280 290 300 NKPHKPTMWT EAWTAWYTGF GIPVPHRPVE DLAYGVAKFI QKGGSFVNYY MYHGGTNFGR 310 320 330 340 350 360 TAGGPFIATS YDYDAPIDEY GLLREPKWGH LKQLHKAIKL CEPALVAGDP IVTSLGNAQK 370 380 390 400 410 420 SSVFRSSTGA CAAFLENKDK VSYARVAFNG MHYDLPPWSI SILPDCKTTV FNTARVGSQI 430 440 450 460 470 480 SQMKMEWAGG FAWQSYNEEI NSFGEDPLTT VGLLEQINVT RDNTDYLWYT TYVDVAQDEQ 490 500 510 520 530 540 FLSNGENLKL TVMSAGHALH IFINGQLKGT VYGSVDDPKL TYTGNVKLWA GSNTISCLSI 550 560 570 580 590 600 AVGLPNVGEH FETWNAGILG PVTLDGLNEG RRDLTWQKWT YQVGLKGESM SLHSLSGSST 610 620 630 640 650 660 VEWGEPVQKQ PLTWYKAFFN APDGDEPLAL DMSSMGKGQI WINGQGIGRY WPGYKASGNC 670 680 690 700 710 720 GTCDYRGEYD ETKCQTNCGD SSQRWYHVPR SWLSPTGNLL VIFEEWGGDP TGISMVKRSI 730 740 750 760 770 780 GSVCADVSEW QPSMKNWHTK DYEKAKVHLQ CDNGQKITEI KFASFGTPQG SCGSYTEGGC 790 800 810 820 HAHKSYDIFW KNCVGQERCG VSVVPEIFGG DPCPGTMKRA VVEAICG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003546 Genomic DNA. Translation: BAB84455.1. AP008207 Genomic DNA. Translation: BAF05319.1. AK103045 mRNA. No translation available.
RefSeq	NP_001043405.1. NM_001049940.1.
UniGene	Os.18562.
3D structure databases
ProteinModelPortal	Q8W0A1.
ModBase	Search...
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Proteomic databases
PRIDE	Q8W0A1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os01g39830.1; LOC_Os01g39830.1; LOC_Os01g39830.
GeneID	4324191.
KEGG	osa:4324191.
NMPDR	fig|39947.1.peg.9948.
Organism-specific databases
Gramene	Q8W0A1.
Phylogenomic databases
GeneTree	EPGT00070000027970.
HOGENOM	HBG318019.
OMA	SWQAYNE.
PhylomeDB	Q8W0A1.
ProtClustDB	CLSN2691526.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_subgr_catalytic. IPR001944. Glycoside_Hdrlase_35. IPR017853. Glycoside_hydrolase_SF. IPR000922. Lectin_gal-bd_dom. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF02140. Gal_Lectin. 1 hit. PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
SUPFAM	SSF49785. Gal_bind_like. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. PS50228. SUEL_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL2_ORYSJ

Accession

Primary (citable) accession number: Q8W0A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	March 1, 2002
Last modified:	December 14, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program