Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8W033

- AL3I1_ARATH

UniProt

Q8W033 - AL3I1_ARATH

Protein

Aldehyde dehydrogenase family 3 member I1, chloroplastic

Gene

ALDH3I1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (31 Oct 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in oxidative stress tolerance by detoxifying reactive aldehydes derived from lipid peroxidation. Medium- to long-chain saturated aldehydes are preferred substrates, while the short-chain aldehyde propanal is a weak substrate. Can use both NAD+ and NADP+, but the coenzyme preference is substrate dependent.2 Publications

    Catalytic activityi

    An aldehyde + NAD+ + H2O = a carboxylate + NADH.1 Publication

    Enzyme regulationi

    Thiol-based regulation. Inactivation after dimerization under oxidizing conditions.1 Publication

    Kineticsi

    1. KM=8053 µM for propionaldehyde1 Publication
    2. KM=111 µM for hexanal1 Publication
    3. KM=24 µM for octanal1 Publication
    4. KM=7 µM for nonanal1 Publication
    5. KM=1.3 µM for dodecanal1 Publication
    6. KM=151 µM for trans-2-hexenal1 Publication
    7. KM=5.5 µM for trans-2-nonenal1 Publication
    8. KM=21 µM for 4-hydroxynonenal1 Publication
    9. KM=71 µM for NAD+ (in the presence of hexanal as co-substrate)1 Publication
    10. KM=1868 µM for NADP+ (in the presence of hexanal as co-substrate)1 Publication
    11. KM=53 µM for NAD+ (in the presence of trans-2-nonenal as co-substrate)1 Publication
    12. KM=87 µM for NADP+ (in the presence of trans-2-nonenal as co-substrate)1 Publication

    Vmax=10.1 µmol/min/mg enzyme with propionaldehyde as substrate1 Publication

    Vmax=17.3 µmol/min/mg enzyme with hexanal as substrate1 Publication

    Vmax=16.6 µmol/min/mg enzyme with octanal as substrate1 Publication

    Vmax=20 µmol/min/mg enzyme with nonanal as substrate1 Publication

    Vmax=18.8 µmol/min/mg enzyme with dodecanal as substrate1 Publication

    Vmax=1.5 µmol/min/mg enzyme with trans-2-hexenal as substrate1 Publication

    Vmax=1.6 µmol/min/mg enzyme with trans-2-nonenal as substrate1 Publication

    Vmax=0.6 µmol/min/mg enzyme with 4-hydroxynonenal as substrate1 Publication

    pH dependencei

    Optimum pH is 9.0.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei186 – 1861Transition state stabilizerBy similarity
    Active sitei281 – 2811Proton acceptorPROSITE-ProRule annotation
    Active sitei316 – 3161NucleophilePROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi259 – 2646NADBy similarity

    GO - Molecular functioni

    1. 3-chloroallyl aldehyde dehydrogenase activity Source: TAIR
    2. aldehyde dehydrogenase (NAD) activity Source: TAIR
    3. aldehyde dehydrogenase (NADP+) activity Source: TAIR
    4. aldehyde dehydrogenase [NAD(P)+] activity Source: InterPro

    GO - Biological processi

    1. cellular aldehyde metabolic process Source: InterPro
    2. response to abscisic acid Source: TAIR
    3. response to water deprivation Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Stress response

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciARA:AT4G34240-MONOMER.
    ARA:GQT-2498-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aldehyde dehydrogenase family 3 member I1, chloroplastic (EC:1.2.1.3)
    Short name:
    AtALDH3
    Short name:
    Ath-ALDH3
    Gene namesi
    Name:ALDH3I1
    Synonyms:ALDH3
    Ordered Locus Names:At4g34240
    ORF Names:F10M10.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G34240.

    Subcellular locationi

    Plastidchloroplast Curated

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast envelope Source: TAIR
    3. plastid Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi114 – 1141C → S: No effect on solubility, but loss of dimerization and 80% loss of activity. 1 Publication
    Mutagenesisi142 – 1421C → S: No effect on solubility, but decreased activity. 1 Publication
    Mutagenesisi263 – 2631V → I: No effect on substrate specificity, but decreased affinity for NADP(+) and increased affinity for NAD(+). 1 Publication
    Mutagenesisi286 – 2861C → S: No effect on solubility, but no effect on activity. 1 Publication
    Mutagenesisi310 – 3101C → S: No effect on solubility, but no effect on activity. 1 Publication
    Mutagenesisi316 – 3161C → S: No effect on solubility, but loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5959ChloroplastSequence AnalysisAdd
    BLAST
    Chaini60 – 550491Aldehyde dehydrogenase family 3 member I1, chloroplasticPRO_0000256061Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi114 – 114Interchain

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ8W033.
    PRIDEiQ8W033.

    Expressioni

    Inductioni

    By abscisic acid (ABA), dehydration, salt stress in plantlets. Induced by heavy metals and H2O2.3 Publications

    Gene expression databases

    ArrayExpressiQ8W033.
    GenevestigatoriQ8W033.

    Interactioni

    Subunit structurei

    Homodimer and homomultimer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ8W033.
    SMRiQ8W033. Positions 31-519.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldehyde dehydrogenase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1012.
    HOGENOMiHOG000271515.
    InParanoidiQ8W033.
    KOiK00128.
    OMAiVARIIMA.
    PhylomeDBiQ8W033.

    Family and domain databases

    Gene3Di3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR012394. Aldehyde_DH_NAD(P).
    [Graphical view]
    PfamiPF00171. Aldedh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036492. ALDH. 1 hit.
    SUPFAMiSSF53720. SSF53720. 1 hit.
    PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q8W033-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTKLLEINHI QTLCFAKGFS PARLNVATSP FRISRRGGGG YCSNACIPYR    50
    LKFTCYATLS AVVKEQASDF SGKEAALLVD ELRSNFNSGR TKSYEWRISQ 100
    LQNIARMIDE KEKCITEALY QDLSKPELEA FLAEISNTKS SCMLAIKELK 150
    NWMAPETVKT SVTTFPSSAQ IVSEPLGVVL VISAWNFPFL LSVEPVIGAI 200
    AAGNAVVLKP SEIAPAASSL LAKLFSEYLD NTTIRVIEGG VPETTALLDQ 250
    KWDKIFFTGG ARVARIIMAA AARNLTPVVL ELGGKCPALV DSDVNLQVAA 300
    RRIIAGKWAC NSGQACIGVD YVITTKDFAS KLIDALKTEL ETFFGQNALE 350
    SKDLSRIVNS FHFKRLESML KENGVANKIV HGGRITEDKL KISPTILLDV 400
    PEASSMMQEE IFGPLLPIIT VQKIEDGFQV IRSKPKPLAA YLFTNNKELE 450
    KQFVQDVSAG GITINDTVLH VTVKDLPFGG VGESGIGAYH GKFSYETFSH 500
    KKGVLYRSFS GDADLRYPPY TPKKKMVLKA LLSSNIFAAI LAFFGFSKDS 550
    Length:550
    Mass (Da):60,173
    Last modified:October 31, 2006 - v2
    Checksum:i42FC2BC37757A083
    GO

    Sequence cautioni

    The sequence CAB36701.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAB80141.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti32 – 321R → L in CAC84903. (PubMed:11849595)Curated
    Sequence conflicti71 – 711S → R in CAC84903. (PubMed:11849595)Curated
    Sequence conflicti435 – 4351P → S in CAC84903. (PubMed:11849595)Curated
    Sequence conflicti536 – 5361I → M in CAC84903. (PubMed:11849595)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ306961 mRNA. Translation: CAC84903.1.
    AL035521 Genomic DNA. Translation: CAB36701.1. Sequence problems.
    AL161585 Genomic DNA. Translation: CAB80141.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE86347.1.
    AY054633 mRNA. Translation: AAK96824.1.
    AY081532 mRNA. Translation: AAM10094.1.
    PIRiT04770.
    RefSeqiNP_567962.1. NM_119588.5. [Q8W033-1]
    UniGeneiAt.26454.

    Genome annotation databases

    EnsemblPlantsiAT4G34240.1; AT4G34240.1; AT4G34240. [Q8W033-1]
    GeneIDi829573.
    KEGGiath:AT4G34240.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ306961 mRNA. Translation: CAC84903.1 .
    AL035521 Genomic DNA. Translation: CAB36701.1 . Sequence problems.
    AL161585 Genomic DNA. Translation: CAB80141.1 . Sequence problems.
    CP002687 Genomic DNA. Translation: AEE86347.1 .
    AY054633 mRNA. Translation: AAK96824.1 .
    AY081532 mRNA. Translation: AAM10094.1 .
    PIRi T04770.
    RefSeqi NP_567962.1. NM_119588.5. [Q8W033-1 ]
    UniGenei At.26454.

    3D structure databases

    ProteinModelPortali Q8W033.
    SMRi Q8W033. Positions 31-519.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q8W033.
    PRIDEi Q8W033.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G34240.1 ; AT4G34240.1 ; AT4G34240 . [Q8W033-1 ]
    GeneIDi 829573.
    KEGGi ath:AT4G34240.

    Organism-specific databases

    TAIRi AT4G34240.

    Phylogenomic databases

    eggNOGi COG1012.
    HOGENOMi HOG000271515.
    InParanoidi Q8W033.
    KOi K00128.
    OMAi VARIIMA.
    PhylomeDBi Q8W033.

    Enzyme and pathway databases

    BioCyci ARA:AT4G34240-MONOMER.
    ARA:GQT-2498-MONOMER.

    Gene expression databases

    ArrayExpressi Q8W033.
    Genevestigatori Q8W033.

    Family and domain databases

    Gene3Di 3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR012394. Aldehyde_DH_NAD(P).
    [Graphical view ]
    Pfami PF00171. Aldedh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036492. ALDH. 1 hit.
    SUPFAMi SSF53720. SSF53720. 1 hit.
    PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Novel ABA- and dehydration-inducible aldehyde dehydrogenase genes isolated from the resurrection plant Craterostigma plantagineum and Arabidopsis thaliana."
      Kirch H.-H., Nair A., Bartels D.
      Plant J. 28:555-567(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
    2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Overexpression of a stress-inducible aldehyde dehydrogenase gene from Arabidopsis thaliana in transgenic plants improves stress tolerance."
      Sunkar R., Bartels D., Kirch H.-H.
      Plant J. 35:452-464(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    6. Cited for: NOMENCLATURE.
    7. "Detailed expression analysis of selected genes of the aldehyde dehydrogenase(ALDH) gene superfamily in Arabidopsis thaliana."
      Kirch H.-H., Schlingensiepen S., Kotchoni S., Sunkar R., Bartels D.
      Plant Mol. Biol. 57:315-332(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    8. "Engineering the nucleotide coenzyme specificity and sulfhydryl redox sensitivity of two stress-responsive aldehyde dehydrogenase isoenzymes of Arabidopsis thaliana."
      Stiti N., Adewale I.O., Petersen J., Bartels D., Kirch H.H.
      Biochem. J. 434:459-471(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, MUTAGENESIS OF CYS-114; CYS-142; VAL-263; CYS-286; CYS-310 AND CYS-316, ENZYME REGULATION, 3D-STRUCTURE MODELING.
    9. "Aldehyde dehydrogenase enzyme ALDH3H1 from Arabidopsis thaliana: Identification of amino acid residues critical for cofactor specificity."
      Stiti N., Podgorska K., Bartels D.
      Biochim. Biophys. Acta 1844:681-693(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING.

    Entry informationi

    Entry nameiAL3I1_ARATH
    AccessioniPrimary (citable) accession number: Q8W033
    Secondary accession number(s): Q940H4, Q9SYY9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: October 31, 2006
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Plants overexpressing ALDH3I1 show improved tolerance when exposed to dehydration, salt stress, heavy metals and H2O2.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3