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Protein

Aldehyde dehydrogenase family 3 member I1, chloroplastic

Gene

ALDH3I1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in oxidative stress tolerance by detoxifying reactive aldehydes derived from lipid peroxidation. Medium- to long-chain saturated aldehydes are preferred substrates, while the short-chain aldehyde propanal is a weak substrate. Can use both NAD+ and NADP+, but the coenzyme preference is substrate dependent.2 Publications

Catalytic activityi

An aldehyde + NAD+ + H2O = a carboxylate + NADH.1 Publication

Enzyme regulationi

Thiol-based regulation. Inactivation after dimerization under oxidizing conditions.1 Publication

Kineticsi

  1. KM=8053 µM for propionaldehyde1 Publication
  2. KM=111 µM for hexanal1 Publication
  3. KM=24 µM for octanal1 Publication
  4. KM=7 µM for nonanal1 Publication
  5. KM=1.3 µM for dodecanal1 Publication
  6. KM=151 µM for trans-2-hexenal1 Publication
  7. KM=5.5 µM for trans-2-nonenal1 Publication
  8. KM=21 µM for 4-hydroxynonenal1 Publication
  9. KM=71 µM for NAD+ (in the presence of hexanal as co-substrate)1 Publication
  10. KM=1868 µM for NADP+ (in the presence of hexanal as co-substrate)1 Publication
  11. KM=53 µM for NAD+ (in the presence of trans-2-nonenal as co-substrate)1 Publication
  12. KM=87 µM for NADP+ (in the presence of trans-2-nonenal as co-substrate)1 Publication
  1. Vmax=10.1 µmol/min/mg enzyme with propionaldehyde as substrate1 Publication
  2. Vmax=17.3 µmol/min/mg enzyme with hexanal as substrate1 Publication
  3. Vmax=16.6 µmol/min/mg enzyme with octanal as substrate1 Publication
  4. Vmax=20 µmol/min/mg enzyme with nonanal as substrate1 Publication
  5. Vmax=18.8 µmol/min/mg enzyme with dodecanal as substrate1 Publication
  6. Vmax=1.5 µmol/min/mg enzyme with trans-2-hexenal as substrate1 Publication
  7. Vmax=1.6 µmol/min/mg enzyme with trans-2-nonenal as substrate1 Publication
  8. Vmax=0.6 µmol/min/mg enzyme with 4-hydroxynonenal as substrate1 Publication

pH dependencei

Optimum pH is 9.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei186Transition state stabilizerBy similarity1
Active sitei281Proton acceptorPROSITE-ProRule annotation1
Active sitei316NucleophilePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi259 – 264NADBy similarity6

GO - Molecular functioni

  • 3-chloroallyl aldehyde dehydrogenase activity Source: TAIR
  • aldehyde dehydrogenase (NAD) activity Source: TAIR
  • aldehyde dehydrogenase (NADP+) activity Source: TAIR
  • aldehyde dehydrogenase [NAD(P)+] activity Source: InterPro

GO - Biological processi

  • cellular aldehyde metabolic process Source: InterPro
  • response to abscisic acid Source: TAIR
  • response to water deprivation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Stress response

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciARA:AT4G34240-MONOMER.
ReactomeiR-ATH-1660661. Sphingolipid de novo biosynthesis.
R-ATH-211945. Phase 1 - Functionalization of compounds.
R-ATH-389599. Alpha-oxidation of phytanate.
R-ATH-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldehyde dehydrogenase family 3 member I1, chloroplastic (EC:1.2.1.3)
Short name:
AtALDH3
Short name:
Ath-ALDH3
Gene namesi
Name:ALDH3I1
Synonyms:ALDH3
Ordered Locus Names:At4g34240
ORF Names:F10M10.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G34240.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi114C → S: No effect on solubility, but loss of dimerization and 80% loss of activity. 1 Publication1
Mutagenesisi142C → S: No effect on solubility, but decreased activity. 1 Publication1
Mutagenesisi263V → I: No effect on substrate specificity, but decreased affinity for NADP(+) and increased affinity for NAD(+). 1 Publication1
Mutagenesisi286C → S: No effect on solubility, but no effect on activity. 1 Publication1
Mutagenesisi310C → S: No effect on solubility, but no effect on activity. 1 Publication1
Mutagenesisi316C → S: No effect on solubility, but loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 59ChloroplastSequence analysisAdd BLAST59
ChainiPRO_000025606160 – 550Aldehyde dehydrogenase family 3 member I1, chloroplasticAdd BLAST491

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi114Interchain

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ8W033.

PTM databases

iPTMnetiQ8W033.

Expressioni

Inductioni

By abscisic acid (ABA), dehydration, salt stress in plantlets. Induced by heavy metals and H2O2.3 Publications

Gene expression databases

ExpressionAtlasiQ8W033. baseline and differential.
GenevisibleiQ8W033. AT.

Interactioni

Subunit structurei

Homodimer and homomultimer.1 Publication

Protein-protein interaction databases

STRINGi3702.AT4G34240.1.

Structurei

3D structure databases

ProteinModelPortaliQ8W033.
SMRiQ8W033.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2456. Eukaryota.
COG1012. LUCA.
HOGENOMiHOG000271515.
InParanoidiQ8W033.
KOiK00128.
OMAiAGKWACN.
OrthoDBiEOG093609F3.
PhylomeDBiQ8W033.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR012394. Aldehyde_DH_NAD(P).
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036492. ALDH. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q8W033-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTKLLEINHI QTLCFAKGFS PARLNVATSP FRISRRGGGG YCSNACIPYR
60 70 80 90 100
LKFTCYATLS AVVKEQASDF SGKEAALLVD ELRSNFNSGR TKSYEWRISQ
110 120 130 140 150
LQNIARMIDE KEKCITEALY QDLSKPELEA FLAEISNTKS SCMLAIKELK
160 170 180 190 200
NWMAPETVKT SVTTFPSSAQ IVSEPLGVVL VISAWNFPFL LSVEPVIGAI
210 220 230 240 250
AAGNAVVLKP SEIAPAASSL LAKLFSEYLD NTTIRVIEGG VPETTALLDQ
260 270 280 290 300
KWDKIFFTGG ARVARIIMAA AARNLTPVVL ELGGKCPALV DSDVNLQVAA
310 320 330 340 350
RRIIAGKWAC NSGQACIGVD YVITTKDFAS KLIDALKTEL ETFFGQNALE
360 370 380 390 400
SKDLSRIVNS FHFKRLESML KENGVANKIV HGGRITEDKL KISPTILLDV
410 420 430 440 450
PEASSMMQEE IFGPLLPIIT VQKIEDGFQV IRSKPKPLAA YLFTNNKELE
460 470 480 490 500
KQFVQDVSAG GITINDTVLH VTVKDLPFGG VGESGIGAYH GKFSYETFSH
510 520 530 540 550
KKGVLYRSFS GDADLRYPPY TPKKKMVLKA LLSSNIFAAI LAFFGFSKDS
Length:550
Mass (Da):60,173
Last modified:October 31, 2006 - v2
Checksum:i42FC2BC37757A083
GO

Sequence cautioni

The sequence CAB36701 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80141 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32R → L in CAC84903 (PubMed:11849595).Curated1
Sequence conflicti71S → R in CAC84903 (PubMed:11849595).Curated1
Sequence conflicti435P → S in CAC84903 (PubMed:11849595).Curated1
Sequence conflicti536I → M in CAC84903 (PubMed:11849595).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ306961 mRNA. Translation: CAC84903.1.
AL035521 Genomic DNA. Translation: CAB36701.1. Sequence problems.
AL161585 Genomic DNA. Translation: CAB80141.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86347.1.
AY054633 mRNA. Translation: AAK96824.1.
AY081532 mRNA. Translation: AAM10094.1.
PIRiT04770.
RefSeqiNP_567962.1. NM_119588.6. [Q8W033-1]
UniGeneiAt.26454.

Genome annotation databases

EnsemblPlantsiAT4G34240.1; AT4G34240.1; AT4G34240. [Q8W033-1]
GeneIDi829573.
GrameneiAT4G34240.1; AT4G34240.1; AT4G34240.
KEGGiath:AT4G34240.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ306961 mRNA. Translation: CAC84903.1.
AL035521 Genomic DNA. Translation: CAB36701.1. Sequence problems.
AL161585 Genomic DNA. Translation: CAB80141.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86347.1.
AY054633 mRNA. Translation: AAK96824.1.
AY081532 mRNA. Translation: AAM10094.1.
PIRiT04770.
RefSeqiNP_567962.1. NM_119588.6. [Q8W033-1]
UniGeneiAt.26454.

3D structure databases

ProteinModelPortaliQ8W033.
SMRiQ8W033.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G34240.1.

PTM databases

iPTMnetiQ8W033.

Proteomic databases

PaxDbiQ8W033.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G34240.1; AT4G34240.1; AT4G34240. [Q8W033-1]
GeneIDi829573.
GrameneiAT4G34240.1; AT4G34240.1; AT4G34240.
KEGGiath:AT4G34240.

Organism-specific databases

TAIRiAT4G34240.

Phylogenomic databases

eggNOGiKOG2456. Eukaryota.
COG1012. LUCA.
HOGENOMiHOG000271515.
InParanoidiQ8W033.
KOiK00128.
OMAiAGKWACN.
OrthoDBiEOG093609F3.
PhylomeDBiQ8W033.

Enzyme and pathway databases

BioCyciARA:AT4G34240-MONOMER.
ReactomeiR-ATH-1660661. Sphingolipid de novo biosynthesis.
R-ATH-211945. Phase 1 - Functionalization of compounds.
R-ATH-389599. Alpha-oxidation of phytanate.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ8W033.

Gene expression databases

ExpressionAtlasiQ8W033. baseline and differential.
GenevisibleiQ8W033. AT.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR012394. Aldehyde_DH_NAD(P).
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036492. ALDH. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAL3I1_ARATH
AccessioniPrimary (citable) accession number: Q8W033
Secondary accession number(s): Q940H4, Q9SYY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 31, 2006
Last modified: November 30, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants overexpressing ALDH3I1 show improved tolerance when exposed to dehydration, salt stress, heavy metals and H2O2.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.