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Protein

Abscisic acid receptor PYL1

Gene

PYL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA (PubMed:19407143, PubMed:19855379, PubMed:19893533, PubMed:19898420, PubMed:23844015, PubMed:21658606). Can be activated by both (-)-ABA and (+)-ABA (PubMed:23844015).6 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86ABA2 Publications1
Sitei115Involved in interactions with PP2Cs1 Publication1
Binding sitei171ABA2 Publications1
Sitei182Involved in interactions with PP2CsBy similarity1

GO - Molecular functioni

  • abscisic acid binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • protein phosphatase inhibitor activity Source: UniProtKB
  • receptor activity Source: UniProtKB

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB
  • regulation of protein serine/threonine phosphatase activity Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Protein phosphatase inhibitor, Receptor

Keywords - Biological processi

Abscisic acid signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Abscisic acid receptor PYL1
Alternative name(s):
ABI1-binding protein 6
PYR1-like protein 1
Regulatory components of ABA receptor 9
Gene namesi
Name:PYL1
Synonyms:RCAR12
Ordered Locus Names:At5g46790
ORF Names:MZA15.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G46790.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi87H → A: Normal affinity for ABI1. Forms monomers and exhibits normal ABA affinity; when associated by A-88 and S-90. 2 Publications1
Mutagenesisi88F → A: Reduced affinity for ABI1. Forms monomers and exhibits normal ABA affinity; when associated by A-87 and S-90. 2 Publications1
Mutagenesisi90K → S: Forms monomers and exhibits normal ABA affinity; when associated by A-87 and A-88. 1 Publication1
Mutagenesisi111I → A: Normal affinity for ABI1. 1 Publication1
Mutagenesisi111I → K: Forms monomer and exhibits an enhanced ABA affinity. 1 Publication1
Mutagenesisi112S → A: Reduced binding affinity and inhibitory activity toward ABI1. 1 Publication1
Mutagenesisi112S → R: Forms homodimer and exhibits an enhanced ABA affinity; when associated with R-117. 1 Publication1
Mutagenesisi114L → A: Reduced affinity for ABI1. 1 Publication1
Mutagenesisi115P → A: Reduced affinity for ABI1. 1 Publication1
Mutagenesisi117N → R: Forms homodimer and exhibits an enhanced ABA affinity; when associated with R-112. 1 Publication1
Mutagenesisi142H → A: Loss of affinity for ABI1. 1 Publication1
Mutagenesisi143R → A: Loss of affinity for ABI1. 1 Publication1
Mutagenesisi144L → A: Loss of affinity for ABI1. 1 Publication1
Mutagenesisi178P → A: Normal affinity for ABI1. 1 Publication1
Mutagenesisi181N → A: Reduced affinity for ABI1. 1 Publication1
Mutagenesisi189F → A: Reduced affinity for ABI1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00003917362 – 221Abscisic acid receptor PYL1Add BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ8VZS8.

PTM databases

iPTMnetiQ8VZS8.

Expressioni

Gene expression databases

GenevisibleiQ8VZS8. AT.

Interactioni

Subunit structurei

Homodimer (PubMed:21658606,PubMed:19898420). Binds ABA on one subunit only. Interacts with HAB1, ABI1 and ABI2, and possibly with other PP2Cs (PubMed:19407142, PubMed:19855379, PubMed:19874541, PubMed:19893533, PubMed:19898420). Binds to CARs protein in an ABA-independent manner, both at the plasma membrane and in the nucleus. Interacts directly with CAR1 and CAR4 (PubMed:25465408).7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HAB1Q9CAJ02EBI-2363104,EBI-2309302

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi19970. 7 interactors.
DIPiDIP-48581N.
IntActiQ8VZS8. 3 interactors.
MINTiMINT-8299470.
STRINGi3702.AT5G46790.1.

Structurei

Secondary structure

1221
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi29 – 31Combined sources3
Helixi34 – 47Combined sources14
Beta strandi56 – 67Combined sources12
Helixi69 – 76Combined sources8
Helixi82 – 84Combined sources3
Beta strandi89 – 93Combined sources5
Beta strandi105 – 110Combined sources6
Beta strandi113 – 115Combined sources3
Beta strandi117 – 127Combined sources11
Turni128 – 131Combined sources4
Beta strandi132 – 143Combined sources12
Beta strandi148 – 176Combined sources29
Helixi183 – 208Combined sources26

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JRQX-ray2.10B28-210[»]
3JRSX-ray2.05A/B/C8-211[»]
3KAYX-ray2.40A/B36-211[»]
3KDJX-ray1.88A20-221[»]
3NEFX-ray2.40A/B20-221[»]
3NEGX-ray2.80A/B20-221[»]
3NMNX-ray2.15A/C36-211[»]
ProteinModelPortaliQ8VZS8.
SMRiQ8VZS8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8VZS8.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 206START-likeAdd BLAST157
Regioni116 – 121ABA binding2 Publications6
Regioni143 – 149ABA binding2 Publications7

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi112 – 116Gate loop1 Publication5
Motifi142 – 144Latch loop1 Publication3

Domaini

Upon interaction with ABA, the 'latch' and 'gate' loops change in conformation leading to a tight dimerization and the creation a surface that enables the receptor to dock into and inhibit the PP2C active site.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IJIR. Eukaryota.
ENOG410YDHK. LUCA.
HOGENOMiHOG000238422.
InParanoidiQ8VZS8.
KOiK14496.
OMAiIHAPPET.
OrthoDBiEOG09360NRL.
PhylomeDBiQ8VZS8.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
InterProiIPR019587. Polyketide_cyclase/dehydratase.
IPR023393. START-like_dom.
[Graphical view]
PfamiPF10604. Polyketide_cyc2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VZS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANSESSSSP VNEEENSQRI STLHHQTMPS DLTQDEFTQL SQSIAEFHTY
60 70 80 90 100
QLGNGRCSSL LAQRIHAPPE TVWSVVRRFD RPQIYKHFIK SCNVSEDFEM
110 120 130 140 150
RVGCTRDVNV ISGLPANTSR ERLDLLDDDR RVTGFSITGG EHRLRNYKSV
160 170 180 190 200
TTVHRFEKEE EEERIWTVVL ESYVVDVPEG NSEEDTRLFA DTVIRLNLQK
210 220
LASITEAMNR NNNNNNSSQV R
Length:221
Mass (Da):25,361
Last modified:March 1, 2002 - v1
Checksum:iB64B1A52C43072C2
GO

Sequence cautioni

The sequence BAB08923 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016882 Genomic DNA. Translation: BAB08923.1. Different initiation.
CP002688 Genomic DNA. Translation: AED95426.1.
AY063877 mRNA. Translation: AAL36233.1.
AY117328 mRNA. Translation: AAM51403.1.
RefSeqiNP_199491.2. NM_124049.5.
UniGeneiAt.27159.

Genome annotation databases

EnsemblPlantsiAT5G46790.1; AT5G46790.1; AT5G46790.
GeneIDi834722.
GrameneiAT5G46790.1; AT5G46790.1; AT5G46790.
KEGGiath:AT5G46790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016882 Genomic DNA. Translation: BAB08923.1. Different initiation.
CP002688 Genomic DNA. Translation: AED95426.1.
AY063877 mRNA. Translation: AAL36233.1.
AY117328 mRNA. Translation: AAM51403.1.
RefSeqiNP_199491.2. NM_124049.5.
UniGeneiAt.27159.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JRQX-ray2.10B28-210[»]
3JRSX-ray2.05A/B/C8-211[»]
3KAYX-ray2.40A/B36-211[»]
3KDJX-ray1.88A20-221[»]
3NEFX-ray2.40A/B20-221[»]
3NEGX-ray2.80A/B20-221[»]
3NMNX-ray2.15A/C36-211[»]
ProteinModelPortaliQ8VZS8.
SMRiQ8VZS8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19970. 7 interactors.
DIPiDIP-48581N.
IntActiQ8VZS8. 3 interactors.
MINTiMINT-8299470.
STRINGi3702.AT5G46790.1.

PTM databases

iPTMnetiQ8VZS8.

Proteomic databases

PaxDbiQ8VZS8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G46790.1; AT5G46790.1; AT5G46790.
GeneIDi834722.
GrameneiAT5G46790.1; AT5G46790.1; AT5G46790.
KEGGiath:AT5G46790.

Organism-specific databases

TAIRiAT5G46790.

Phylogenomic databases

eggNOGiENOG410IJIR. Eukaryota.
ENOG410YDHK. LUCA.
HOGENOMiHOG000238422.
InParanoidiQ8VZS8.
KOiK14496.
OMAiIHAPPET.
OrthoDBiEOG09360NRL.
PhylomeDBiQ8VZS8.

Miscellaneous databases

EvolutionaryTraceiQ8VZS8.
PROiQ8VZS8.

Gene expression databases

GenevisibleiQ8VZS8. AT.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
InterProiIPR019587. Polyketide_cyclase/dehydratase.
IPR023393. START-like_dom.
[Graphical view]
PfamiPF10604. Polyketide_cyc2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYL1_ARATH
AccessioniPrimary (citable) accession number: Q8VZS8
Secondary accession number(s): Q9FIP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 2, 2010
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The synthetic growth inhibitor pyrabactin inhibits ABA-binding and subsequent PP2Cs inhibitor properties.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.