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Q8VZS8 (PYL1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Abscisic acid receptor PYL1
Alternative name(s):
ABI1-binding protein 6
PYR1-like protein 1
Regulatory components of ABA receptor 9
Gene names
Name:PYL1
Synonyms:RCAR12
Ordered Locus Names:At5g46790
ORF Names:MZA15.21
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length221 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA. Ref.5 Ref.8 Ref.9 Ref.10

Subunit structure

Homodimer. Binds ABA on one subunit only. Interacts with HAB1, ABI1 and ABI2, and possibly with other PP2Cs. Ref.4 Ref.6 Ref.8 Ref.9

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Domain

Upon interaction with ABA, the 'latch' and 'gate' loops change in conformation leading to a tight dimerization and the creation a surface that enables the receptor to dock into and inhibit the PP2C active site.

Miscellaneous

The synthetic growth inhibitor pyrabactin inhibits ABA-binding and subsequent PP2Cs inhibitor properties.

Sequence similarities

Belongs to the PYR/PYL/RCAR abscisic acid intracellular receptor family.

Sequence caution

The sequence BAB08923.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HAB1Q9CAJ02EBI-2363104,EBI-2309302

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 221221Abscisic acid receptor PYL1
PRO_0000391736

Regions

Region50 – 206157START-like
Region116 – 1216ABA binding
Region143 – 1497ABA binding
Motif112 – 1165Gate loop
Motif142 – 1443Latch loop

Sites

Binding site861ABA
Binding site1711ABA
Site1151Involved in interactions with PP2Cs
Site1821Involved in interactions with PP2Cs By similarity

Experimental info

Mutagenesis871H → A: Normal affinity for ABI1. Ref.9
Mutagenesis881F → A: Reduced affinity for ABI1. Ref.9
Mutagenesis1111I → A: Normal affinity for ABI1. Ref.9
Mutagenesis1121S → A: Reduced binding affinity and inhibitory activity toward ABI1. Ref.9
Mutagenesis1141L → A: Reduced affinity for ABI1. Ref.9
Mutagenesis1151P → A: Reduced affinity for ABI1. Ref.9
Mutagenesis1421H → A: Loss of affinity for ABI1. Ref.9
Mutagenesis1431R → A: Loss of affinity for ABI1. Ref.9
Mutagenesis1441L → A: Loss of affinity for ABI1. Ref.9
Mutagenesis1781P → A: Normal affinity for ABI1. Ref.9
Mutagenesis1811N → A: Reduced affinity for ABI1. Ref.9
Mutagenesis1891F → A: Reduced affinity for ABI1. Ref.9

Secondary structure

......................... 221
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8VZS8 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: B64B1A52C43072C2

FASTA22125,361
        10         20         30         40         50         60 
MANSESSSSP VNEEENSQRI STLHHQTMPS DLTQDEFTQL SQSIAEFHTY QLGNGRCSSL 

        70         80         90        100        110        120 
LAQRIHAPPE TVWSVVRRFD RPQIYKHFIK SCNVSEDFEM RVGCTRDVNV ISGLPANTSR 

       130        140        150        160        170        180 
ERLDLLDDDR RVTGFSITGG EHRLRNYKSV TTVHRFEKEE EEERIWTVVL ESYVVDVPEG 

       190        200        210        220 
NSEEDTRLFA DTVIRLNLQK LASITEAMNR NNNNNNSSQV R 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"PYR/PYL/RCAR family members are major in-vivo ABI1 protein phosphatase 2C-interacting proteins in Arabidopsis."
Nishimura N., Sarkeshik A., Nito K., Park S.-Y., Wang A., Carvalho P.C., Lee S., Caddell D.F., Cutler S.R., Chory J., Yates J.R., Schroeder J.I.
Plant J. 61:290-299(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ABI1, IDENTIFICATION BY MASS SPECTROMETRY.
[5]"Regulators of PP2C phosphatase activity function as abscisic acid sensors."
Ma Y., Szostkiewicz I., Korte A., Moes D., Yang Y., Christmann A., Grill E.
Science 324:1064-1068(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, GENE FAMILY.
[6]"Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins."
Park S.-Y., Fung P., Nishimura N., Jensen D.R., Fujii H., Zhao Y., Lumba S., Santiago J., Rodrigues A., Chow T.F., Alfred S.E., Bonetta D., Finkelstein R., Provart N.J., Desveaux D., Rodriguez P.L., McCourt P., Zhu J.-K. expand/collapse author list , Schroeder J.I., Volkman B.F., Cutler S.R.
Science 324:1068-1071(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HAB1, GENE FAMILY, NOMENCLATURE.
[7]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors."
Melcher K., Ng L.-M., Zhou X.E., Soon F.-F., Xu Y., Suino-Powell K.M., Park S.-Y., Weiner J.J., Fujii H., Chinnusamy V., Kovach A., Li J., Wang Y., Li J., Peterson F.C., Jensen D.R., Yong E.-L., Volkman B.F. expand/collapse author list , Cutler S.R., Zhu J.-K., Xu H.E.
Nature 462:602-608(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 36-211, DIMERIZATION, INTERACTION WITH HAB1; ABI1 AND ABI2, FUNCTION.
[9]"Structural basis of abscisic acid signalling."
Miyazono K.-I., Miyakawa T., Sawano Y., Kubota K., Kang H.-J., Asano A., Miyauchi Y., Takahashi M., Zhi Y., Fujita Y., Yoshida T., Kodaira K.-S., Yamaguchi-Shinozaki K., Tanokura M.
Nature 462:609-614(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 8-211 IN COMPLEX WITH ABSCISIC ACID AND PP2C ABI1, INTERACTION WITH ABA AND ABI1, MUTAGENESIS OF HIS-87; PHE-88; ILE-111; SER-112; LEU-114; PRO-115; HIS-142; ARG-143; LEU-144; PRO-178; ASN-181 AND PHE-189, FUNCTION.
[10]"Structural insights into the mechanism of abscisic acid signaling by PYL proteins."
Yin P., Fan H., Hao Q., Yuan X., Wu D., Pang Y., Yan C., Li W., Wang J., Yan N.
Nat. Struct. Mol. Biol. 16:1230-1236(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.88 ANGSTROMS) OF 20-221 IN COMPLEX WITH ABSCISIC ACID AND PP2C ABI1, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB016882 Genomic DNA. Translation: BAB08923.1. Different initiation.
CP002688 Genomic DNA. Translation: AED95426.1.
AY063877 mRNA. Translation: AAL36233.1.
AY117328 mRNA. Translation: AAM51403.1.
RefSeqNP_199491.2. NM_124049.4.
UniGeneAt.27159.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3JRQX-ray2.10B28-210[»]
3JRSX-ray2.05A/B/C8-211[»]
3KAYX-ray2.40A/B36-211[»]
3KDJX-ray1.88A20-221[»]
3NEFX-ray2.40A/B20-221[»]
3NEGX-ray2.80A/B20-221[»]
3NMNX-ray2.15A/C36-211[»]
ProteinModelPortalQ8VZS8.
SMRQ8VZS8. Positions 31-209.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid19970. 3 interactions.
DIPDIP-48581N.
IntActQ8VZS8. 3 interactions.
MINTMINT-8299470.
STRING3702.AT5G46790.1-P.

Proteomic databases

PaxDbQ8VZS8.
PRIDEQ8VZS8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G46790.1; AT5G46790.1; AT5G46790.
GeneID834722.
KEGGath:AT5G46790.

Organism-specific databases

TAIRAT5G46790.

Phylogenomic databases

eggNOGNOG235377.
HOGENOMHOG000238422.
InParanoidQ8VZS8.
KOK14496.
OMAEEERIWT.
PhylomeDBQ8VZS8.

Gene expression databases

GenevestigatorQ8VZS8.

Family and domain databases

Gene3D3.30.530.20. 1 hit.
InterProIPR019587. Polyketide_cyclase/dehydratase.
IPR023393. START-like_dom.
[Graphical view]
PfamPF10604. Polyketide_cyc2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8VZS8.

Entry information

Entry namePYL1_ARATH
AccessionPrimary (citable) accession number: Q8VZS8
Secondary accession number(s): Q9FIP6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 2, 2010
Last sequence update: March 1, 2002
Last modified: June 11, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names