Alpha-L-arabinofuranosidase 2 precursor - Arabidopsis thaliana (Mouse-ear cress)

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Q8VZR2 (ASD2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 68. History...

Clusters with 100%, 90%, 50% identity |

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alpha-L-arabinofuranosidase 2
Short name=AtASD2
EC=3.2.1.55

Alternative name(s):
Beta-D-xylosidase
EC=3.2.1.-

Gene names

Name:	ASD2
Synonyms:	ARAF2
Ordered Locus Names:	At5g26120
ORF Names:	T1N24.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	674 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in the coordinated dissolution of the cell wall matrix during abscission and in the secondary cell wall formation in xylem vessels. Ref.5
Catalytic activity	Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.
Subcellular location	Secreted › extracellular space › extracellular matrix Potential.
Tissue specificity	High expression in flowers, siliques and stems. Observed in the vasculature of older root tissue, at the tip of anthers and in the petal blade of fully developed flowers, in floral abscission zones and in silique replum tissue. Expressed in the cambium and phloem, but not in the xylem or in the vascular system of floral tissues. Ref.1 Ref.5
Induction	Not induced by hormones or during leaf senescence. Ref.1
Disruption phenotype	No developmental phenotype, even in a asd1/asd2 double mutant. Ref.5
Sequence similarities	Belongs to the glycosyl hydrolase 51 family.
Sequence caution	The sequence AAD40132.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-arabinose metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	alpha-N-arabinofuranosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

24

Potential

Chain

650

Alpha-L-arabinofuranosidase 2

PRO_0000384372

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q8VZR2 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: C9FD37B9F397890B
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674

75,439

        10         20         30         40         50         60 
MDMETSWRFL RSVCLLSFIL GSFSVYQTLC LVDAQEDAIV TLQVDASNVT RRPIPETLFG 

        70         80         90        100        110        120 
IFFEEINHAG AGGLWAELVS NRGFEAGGQI IPSNIWPWSI IGDESSIYVV TDRSSCFERN 

       130        140        150        160        170        180 
KIALRMEVLC DSNSCPLGGV GVYNPGYWGM NIEEGKKYKV VLYVRSTGDI DVSVSFTSSN 

       190        200        210        220        230        240 
GSVTLASENI IALASDLLNW TKKEMLLEAN GTDNGARLQF TTTKKGSIWF DQVSAMPMDT 

       250        260        270        280        290        300 
YKGHGFRNDL FQMMVDLKPR FIRFPGGCFV EGDWLGNAFR WKETVRAWEE RPGHYGDVWK 

       310        320        330        340        350        360 
YWTDDGLGHF EFFQLAEDLG ASPIWVFNNG ISHNDQVETK NVMPFVQEAI DGIEFARGDS 

       370        380        390        400        410        420 
NSTWGSVRAA MGHPEPFELK YVAVGNEDCF KSYYRGNYLE FYNAIKKAYP DIKIISNCDA 

       430        440        450        460        470        480 
SAKPLDHPAD YFDYHIYTLA RDLFSKSHDF DNTPRNGPKA FVSEYAVNKA DAKNGNLLAA 

       490        500        510        520        530        540 
LGEAAFLLGL EKNSDIVEMV SYAPLFVNTN DRRWIPDAIV FNSSHLYGTP SYWVQHFFTE 

       550        560        570        580        590        600 
SSGATLLNST LKGKTSSVEA SAISFQTNGK DYIQIKAVNF GEQSVNLKVA VTGLMAKFYG 

       610        620        630        640        650        660 
SKKKVLTSAS VMDENSFSNP NMIVPQESLL EMTEQEDLMF VLPPHSFSSF DLLTESENVI 

       670 
KMPISDSYKK TSTM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two alpha-L-arabinofuranosidase genes in Arabidopsis thaliana are differentially expressed during vegetative growth and flower development." Fulton L.M., Cobbett C.S. J. Exp. Bot. 54:2467-2477(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION BY HORMONES. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Cell wall modifications in Arabidopsis plants with altered alpha-L-arabinofuranosidase activity." Chavez Montes R.A., Ranocha P., Martinez Y., Minic Z., Jouanin L., Marquis M., Saulnier L., Fulton L.M., Cobbett C.S., Bitton F., Renou J.-P., Jauneau A., Goffner D. Plant Physiol. 147:63-77(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY243510 mRNA. Translation: AAO92262.1. AF149413 Genomic DNA. Translation: AAD40132.1. Sequence problems. CP002688 Genomic DNA. Translation: AED93527.1. AY063925 mRNA. Translation: AAL36281.1. BT006062 mRNA. Translation: AAP04047.1.
IPI	IPI00517686.
RefSeq	NP_197984.2. NM_122513.3.
UniGene	At.27144.
3D structure databases
ProteinModelPortal	Q8VZR2.
SMR	Q8VZR2. Positions 228-650.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G26120.1-P.
Protein family/group databases
CAZy	GH51. Glycoside Hydrolase Family 51.
Proteomic databases
PaxDb	Q8VZR2.
PRIDE	Q8VZR2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G26120.1; AT5G26120.1; AT5G26120.
GeneID	832681.
KEGG	ath:AT5G26120.
Organism-specific databases
TAIR	At5g26120.
Phylogenomic databases
eggNOG	COG3534.
HOGENOM	HOG000115340.
InParanoid	Q8VZR2.
OMA	GISHNDQ.
PhylomeDB	Q8VZR2.
ProtClustDB	CLSN2684036.
Gene expression databases
Genevestigator	Q8VZR2.
Family and domain databases
InterPro	IPR010720. Alpha-L-AF_C. IPR008979. Galactose-bd-like. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF06964. Alpha-L-AF_C. 1 hit. [Graphical view]
SMART	SM00813. Alpha-L-AF_C. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASD2_ARATH

Accession

Primary (citable) accession number: Q8VZR2
Secondary accession number(s): Q9XH04

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents