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Protein

MDIS1-interacting receptor like kinase 2

Gene

MIK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the pollen tube perception of the female signal.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei802ATPPROSITE-ProRule annotation1
Active sitei905Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi781 – 789ATPPROSITE-ProRule annotation9

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
MDIS1-interacting receptor like kinase 21 Publication
Short name:
AtMIK21 Publication
Alternative name(s):
Probable LRR receptor-like serine/threonine-protein kinase At4g08850 (EC:2.7.11.1Curated)
Gene namesi
Name:MIK21 Publication
Ordered Locus Names:At4g08850Imported
ORF Names:T32A17.160Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G08850.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini44 – 709ExtracellularSequence analysisAdd BLAST666
Transmembranei710 – 730HelicalSequence analysisAdd BLAST21
Topological domaini731 – 1045CytoplasmicSequence analysisAdd BLAST315

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
  • pollen tube Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 43Sequence analysisAdd BLAST43
ChainiPRO_000038755944 – 1045MDIS1-interacting receptor like kinase 2Add BLAST1002

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi63N-linked (GlcNAc...)Sequence analysis1
Glycosylationi77N-linked (GlcNAc...)Sequence analysis1
Glycosylationi99N-linked (GlcNAc...)Sequence analysis1
Glycosylationi119N-linked (GlcNAc...)Sequence analysis1
Glycosylationi179N-linked (GlcNAc...)Sequence analysis1
Glycosylationi212N-linked (GlcNAc...)Sequence analysis1
Glycosylationi249N-linked (GlcNAc...)Sequence analysis1
Glycosylationi263N-linked (GlcNAc...)Sequence analysis1
Glycosylationi284N-linked (GlcNAc...)Sequence analysis1
Glycosylationi323N-linked (GlcNAc...)Sequence analysis1
Glycosylationi380N-linked (GlcNAc...)Sequence analysis1
Glycosylationi393N-linked (GlcNAc...)Sequence analysis1
Glycosylationi410N-linked (GlcNAc...)Sequence analysis1
Glycosylationi487N-linked (GlcNAc...)Sequence analysis1
Glycosylationi500N-linked (GlcNAc...)Sequence analysis1
Glycosylationi580N-linked (GlcNAc...)Sequence analysis1
Glycosylationi633N-linked (GlcNAc...)Sequence analysis1
Glycosylationi687N-linked (GlcNAc...)Sequence analysis1
Modified residuei772PhosphothreonineBy similarity1
Modified residuei853PhosphotyrosineBy similarity1
Modified residuei892PhosphotyrosineBy similarity1
Modified residuei938PhosphoserineBy similarity1
Modified residuei946PhosphotyrosineBy similarity1
Modified residuei953PhosphotyrosineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8VZG8.
PRIDEiQ8VZG8.

PTM databases

iPTMnetiQ8VZG8.
SwissPalmiQ8VZG8.

Expressioni

Tissue specificityi

Expressed in pollen tubes.1 Publication

Gene expression databases

GenevisibleiQ8VZG8. AT.

Interactioni

Subunit structurei

Interacts with MDIS1 and LURE1.2.1 Publication

Protein-protein interaction databases

BioGridi11755. 3 interactors.
DIPiDIP-53470N.
IntActiQ8VZG8. 1 interactor.
STRINGi3702.AT4G08850.1.

Structurei

3D structure databases

ProteinModelPortaliQ8VZG8.
SMRiQ8VZG8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati92 – 116LRR 1Sequence analysisAdd BLAST25
Repeati117 – 140LRR 2Sequence analysisAdd BLAST24
Repeati141 – 165LRR 3Sequence analysisAdd BLAST25
Repeati166 – 189LRR 4Sequence analysisAdd BLAST24
Repeati191 – 212LRR 5Sequence analysisAdd BLAST22
Repeati213 – 237LRR 6Sequence analysisAdd BLAST25
Repeati238 – 260LRR 7Sequence analysisAdd BLAST23
Repeati262 – 285LRR 8Sequence analysisAdd BLAST24
Repeati286 – 309LRR 9Sequence analysisAdd BLAST24
Repeati311 – 333LRR 10Sequence analysisAdd BLAST23
Repeati334 – 356LRR 11Sequence analysisAdd BLAST23
Repeati357 – 381LRR 12Sequence analysisAdd BLAST25
Repeati383 – 405LRR 13Sequence analysisAdd BLAST23
Repeati406 – 429LRR 14Sequence analysisAdd BLAST24
Repeati431 – 452LRR 15Sequence analysisAdd BLAST22
Repeati453 – 476LRR 16Sequence analysisAdd BLAST24
Repeati477 – 501LRR 17Sequence analysisAdd BLAST25
Repeati502 – 525LRR 18Sequence analysisAdd BLAST24
Repeati527 – 549LRR 19Sequence analysisAdd BLAST23
Repeati550 – 573LRR 20Sequence analysisAdd BLAST24
Repeati575 – 597LRR 21Sequence analysisAdd BLAST23
Repeati598 – 620LRR 22Sequence analysisAdd BLAST23
Repeati621 – 644LRR 23Sequence analysisAdd BLAST24
Repeati646 – 670LRR 24Sequence analysisAdd BLAST25
Domaini775 – 1045Protein kinasePROSITE-ProRule annotationAdd BLAST271

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 24 LRR (leucine-rich) repeats.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
HOGENOMiHOG000116551.
InParanoidiQ8VZG8.
OMAiEVIKGEH.
OrthoDBiEOG093601B3.
PhylomeDBiQ8VZG8.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
3.80.10.10. 4 hits.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00560. LRR_1. 1 hit.
PF13855. LRR_8. 2 hits.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 9 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51450. LRR. 16 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VZG8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNKTNPERKI SLTSFKERMA CKEKPRDLQV LLIISIVLSC SFAVSATVEE
60 70 80 90 100
ANALLKWKST FTNQTSSSKL SSWVNPNTSS FCTSWYGVAC SLGSIIRLNL
110 120 130 140 150
TNTGIEGTFE DFPFSSLPNL TFVDLSMNRF SGTISPLWGR FSKLEYFDLS
160 170 180 190 200
INQLVGEIPP ELGDLSNLDT LHLVENKLNG SIPSEIGRLT KVTEIAIYDN
210 220 230 240 250
LLTGPIPSSF GNLTKLVNLY LFINSLSGSI PSEIGNLPNL RELCLDRNNL
260 270 280 290 300
TGKIPSSFGN LKNVTLLNMF ENQLSGEIPP EIGNMTALDT LSLHTNKLTG
310 320 330 340 350
PIPSTLGNIK TLAVLHLYLN QLNGSIPPEL GEMESMIDLE ISENKLTGPV
360 370 380 390 400
PDSFGKLTAL EWLFLRDNQL SGPIPPGIAN STELTVLQLD TNNFTGFLPD
410 420 430 440 450
TICRGGKLEN LTLDDNHFEG PVPKSLRDCK SLIRVRFKGN SFSGDISEAF
460 470 480 490 500
GVYPTLNFID LSNNNFHGQL SANWEQSQKL VAFILSNNSI TGAIPPEIWN
510 520 530 540 550
MTQLSQLDLS SNRITGELPE SISNINRISK LQLNGNRLSG KIPSGIRLLT
560 570 580 590 600
NLEYLDLSSN RFSSEIPPTL NNLPRLYYMN LSRNDLDQTI PEGLTKLSQL
610 620 630 640 650
QMLDLSYNQL DGEISSQFRS LQNLERLDLS HNNLSGQIPP SFKDMLALTH
660 670 680 690 700
VDVSHNNLQG PIPDNAAFRN APPDAFEGNK DLCGSVNTTQ GLKPCSITSS
710 720 730 740 750
KKSHKDRNLI IYILVPIIGA IIILSVCAGI FICFRKRTKQ IEEHTDSESG
760 770 780 790 800
GETLSIFSFD GKVRYQEIIK ATGEFDPKYL IGTGGHGKVY KAKLPNAIMA
810 820 830 840 850
VKKLNETTDS SISNPSTKQE FLNEIRALTE IRHRNVVKLF GFCSHRRNTF
860 870 880 890 900
LVYEYMERGS LRKVLENDDE AKKLDWGKRI NVVKGVAHAL SYMHHDRSPA
910 920 930 940 950
IVHRDISSGN ILLGEDYEAK ISDFGTAKLL KPDSSNWSAV AGTYGYVAPE
960 970 980 990 1000
LAYAMKVTEK CDVYSFGVLT LEVIKGEHPG DLVSTLSSSP PDATLSLKSI
1010 1020 1030 1040
SDHRLPEPTP EIKEEVLEIL KVALLCLHSD PQARPTMLSI STAFS
Length:1,045
Mass (Da):115,423
Last modified:December 15, 2009 - v3
Checksum:i7C9E44DE99DAE8BF
GO
Isoform 2 (identifier: Q8VZG8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     950-1045: ELAYAMKVTE...TMLSISTAFS → GTLFDPLDKL...LTMGWIWFSF

Note: No experimental confirmation available.
Show »
Length:1,009
Mass (Da):112,115
Checksum:iCFF13C5CC2B0E54D
GO

Sequence cautioni

The sequence AAN60259 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAB78010 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB82121 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10I → S in AAN60259 (Ref. 6) Curated1
Sequence conflicti111D → N in AAN60259 (Ref. 6) Curated1
Sequence conflicti389L → V in CAB78010 (PubMed:10617198).Curated1
Sequence conflicti389L → V in CAB82121 (PubMed:10617198).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038295950 – 1045ELAYA…STAFS → GTLFDPLDKLVVDLTRLWSG RVEIMVRFGLFGLNFNQIKT KMFCFGLKFFLTMGWIWFSF in isoform 2. 2 PublicationsAdd BLAST96

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161513 Genomic DNA. Translation: CAB78010.1. Sequence problems.
AL161813 Genomic DNA. Translation: CAB82121.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE82682.1.
CP002687 Genomic DNA. Translation: AEE82683.1.
AY064972 mRNA. Translation: AAL57627.1.
AK317056 mRNA. Translation: BAH19749.1.
FJ708744 mRNA. Translation: ACN59338.1.
AF083700 mRNA. Translation: AAN60259.1. Different initiation.
PIRiB85089.
RefSeqiNP_192625.4. NM_116955.5. [Q8VZG8-2]
NP_849538.2. NM_179207.3. [Q8VZG8-1]
UniGeneiAt.19749.
At.47927.
At.67694.

Genome annotation databases

EnsemblPlantsiAT4G08850.1; AT4G08850.1; AT4G08850. [Q8VZG8-1]
GeneIDi826456.
GrameneiAT4G08850.1; AT4G08850.1; AT4G08850.
KEGGiath:AT4G08850.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161513 Genomic DNA. Translation: CAB78010.1. Sequence problems.
AL161813 Genomic DNA. Translation: CAB82121.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE82682.1.
CP002687 Genomic DNA. Translation: AEE82683.1.
AY064972 mRNA. Translation: AAL57627.1.
AK317056 mRNA. Translation: BAH19749.1.
FJ708744 mRNA. Translation: ACN59338.1.
AF083700 mRNA. Translation: AAN60259.1. Different initiation.
PIRiB85089.
RefSeqiNP_192625.4. NM_116955.5. [Q8VZG8-2]
NP_849538.2. NM_179207.3. [Q8VZG8-1]
UniGeneiAt.19749.
At.47927.
At.67694.

3D structure databases

ProteinModelPortaliQ8VZG8.
SMRiQ8VZG8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi11755. 3 interactors.
DIPiDIP-53470N.
IntActiQ8VZG8. 1 interactor.
STRINGi3702.AT4G08850.1.

PTM databases

iPTMnetiQ8VZG8.
SwissPalmiQ8VZG8.

Proteomic databases

PaxDbiQ8VZG8.
PRIDEiQ8VZG8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G08850.1; AT4G08850.1; AT4G08850. [Q8VZG8-1]
GeneIDi826456.
GrameneiAT4G08850.1; AT4G08850.1; AT4G08850.
KEGGiath:AT4G08850.

Organism-specific databases

TAIRiAT4G08850.

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
HOGENOMiHOG000116551.
InParanoidiQ8VZG8.
OMAiEVIKGEH.
OrthoDBiEOG093601B3.
PhylomeDBiQ8VZG8.

Miscellaneous databases

PROiQ8VZG8.

Gene expression databases

GenevisibleiQ8VZG8. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
3.80.10.10. 4 hits.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00560. LRR_1. 1 hit.
PF13855. LRR_8. 2 hits.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 9 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51450. LRR. 16 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMIK2_ARATH
AccessioniPrimary (citable) accession number: Q8VZG8
Secondary accession number(s): B9DG82, Q8H7F4, Q9LCZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: December 15, 2009
Last modified: November 30, 2016
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.