Reviewed, UniProtKB/Swiss-Prot Q8VZC3 (AL121_ARATH)

Names and origin

Protein names

Recommended name:
    Delta-1-pyrroline-5-carboxylate dehydrogenase 12A1, mitochondrial
      Short name=P5C dehydrogenase
      Short name=AtP5CDH
    EC=1.5.1.12
Alternative name(s):
    Aldehyde dehydrogenase family 12 member A1

Gene names

Name:	ALDH12A1
Synonyms:	P5CDH
Ordered Locus Names:	At5g62530
ORF Names:	K19B1.14

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.
Subcellular location	Mitochondrion matrix Probable.
Tissue specificity	Highly expressed in flowers. Constitutively expressed at low levels in the other tissues. Ref.1
Induction	By treatment with proline. Ref.1
Sequence similarities	Belongs to the aldehyde dehydrogenase family.
Sequence caution	The sequence BAB11503.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxygen and reactive oxygen species metabolic process Inferred from mutant phenotype. Source: TAIR proline catabolic process to glutamate Inferred from mutant phenotype. Source: TAIR response to salt stress Inferred from expression pattern. Source: TAIR
Cellular component	chloroplast Inferred from direct assay. Source: TAIR mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Ref.1 Inferred from genetic interaction. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 556		Delta-1-pyrroline-5-carboxylate dehydrogenase 12A1, mitochondrial		PRO_0000256062
Regions
	Nucleotide binding	282 – 287	6	NAD By similarity
Sites
	Active site	301	1	Proton acceptor By similarity
	Active site	336	1	Nucleophile By similarity
	Site	207	1	Transition state stabilizer By similarity
Experimental info
	Sequence conflict	48	1	A → S in AAK73756. Ref.1
	Sequence conflict	462	1	E → D in AAK73756. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8VZC3-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 83F19E7A300D5565	FASTA	556	61,773
	10 20 30 40 50 60 MYRVFASRAL RAKSLCDKSS TSLASLTLSR LNHSIPFATV DAEELSGAHP AEVQSFVQGK 70 80 90 100 110 120 WIGSSNHNTL LDPLNGEPFI KVAEVDESGT QPFVDSLSQC PKHGLHNPFK SPERYLLYGD 130 140 150 160 170 180 ISTKAAHMLA LPKVADFFAR LIQRVAPKSY QQAAGEVFVT RKFLENFCGD QVRFLARSFA 190 200 210 220 230 240 IPGNHLGQQS HGYRWPYGPV TIVTPFNFPL EIPLLQLMGA LYMGNKPLLK VDSKVSIVME 250 260 270 280 290 300 QMMRLLHYCG LPAEDVDFIN SDGKTMNKIL LEANPRMTLF TGSSRVAEKL ALDLKGRIRL 310 320 330 340 350 360 EDAGFDWKVL GPDVQEVDYV AWQCDQDAYA CSGQKCSAQS MLFVHENWSK TPLVSKLKEL 370 380 390 400 410 420 AERRKLEDLT IGPVLTFTTE AMLEHMENLL QIPGSKLLFG GKELKNHSIP SIYGALEPTA 430 440 450 460 470 480 VYVPIEEILK DNKTYELVTK EIFGPFQIVT EYKKDQLPLV LEALERMHAH LTAAVVSNDP 490 500 510 520 530 540 IFLQEVIGNS VNGTTYAGLR GRTTGAPQNH WFGPAGDPRG AGIGTPEAIK LVWSCHREVI 550 YDYGPVPQGW ELPPST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A nuclear gene encoding mitochondrial delta-1-pyrroline-5-carboxylate dehydrogenase and its potential role in protection from proline toxicity." Deuschle K., Funck D., Hellmann H., Daeschner K., Binder S., Frommer W.B. Plant J. 27:345-356(2001) [PubMed: 11532180] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones." Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S. DNA Res. 5:297-308(1998) [PubMed: 9872454] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The ALDH gene superfamily of Arabidopsis." Kirch H.-H., Bartels D., Wei Y., Schnable P.S., Wood A.J. Trends Plant Sci. 9:371-377(2004) [PubMed: 15358267] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY039787 mRNA. Translation: AAK73756.1. AB015469 Genomic DNA. Translation: BAB11503.1. Sequence problems. AY065072 mRNA. Translation: AAL38248.1. BT000746 mRNA. Translation: AAN31887.1. BT010391 mRNA. Translation: AAQ56834.1.
IPI	IPI00520602.
RefSeq	NP_568955.1.
UniGene	At.73901 At.9309
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q8VZC3.
Genome annotation databases
GeneID	836373.
GenomeReviews	Gene locus AT5G62530 in contig BA000015_GR.
KEGG	ath:AT5G62530.
NMPDR	fig|3702.1.peg.28292.
Organism-specific databases
GeneFarm	4315.
TAIR	At5g62530.
Phylogenomic databases
OMA	Q8VZC3. NFPLEIP.
Enzyme and pathway databases
BRENDA	1.5.1.12. 302.
Gene expression databases
ArrayExpress	Q8VZC3.
GermOnline	AT5G62530. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AL121_ARATH

Accession

Primary (citable) accession number: Q8VZC3
Secondary accession number(s): Q93Y55, Q9FJJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	March 1, 2002
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)