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Protein

Alpha-amylase 1

Gene

AMY1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possesses alpha-amylase activity in vitro, but seems not required for breakdown of transitory starch in leaves.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.1 Publication

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi116Calcium 1By similarity1
Metal bindingi133Calcium 2By similarity1
Metal bindingi136Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi138Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi142Calcium 2By similarity1
Metal bindingi152Calcium 3By similarity1
Metal bindingi162Calcium 1By similarity1
Metal bindingi166Calcium 3By similarity1
Metal bindingi167Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi170Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi172Calcium 1By similarity1
Metal bindingi172Calcium 3By similarity1
Active sitei203NucleophileBy similarity1
Metal bindingi207Calcium 1; via carbonyl oxygenBy similarity1
Active sitei228Proton donorBy similarity1
Binding sitei230SubstrateBy similarity1
Binding sitei292SubstrateBy similarity1
Binding sitei311SubstrateBy similarity1
Sitei312Transition state stabilizerBy similarity1
Binding sitei393SubstrateBy similarity1
Binding sitei420SubstrateBy similarity1

GO - Molecular functioni

  • alpha-amylase activity Source: TAIR
  • calcium ion binding Source: InterPro

GO - Biological processi

  • carbohydrate metabolic process Source: UniProtKB-KW
  • response to abscisic acid Source: TAIR
  • response to gibberellin Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciARA:AT4G25000-MONOMER.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 1 (EC:3.2.1.11 Publication)
Short name:
AtAMY1
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:AMY1
Ordered Locus Names:At4g25000
ORF Names:F13M23.140
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G25000.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • extracellular region Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

Pathology & Biotechi

Disruption phenotypei

Early flowering.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000041886125 – 423Alpha-amylase 1Add BLAST399

Proteomic databases

PaxDbiQ8VZ56.
ProMEXiQ8VZ56.

Expressioni

Tissue specificityi

Expressed in leaves, stems, flowers and developing siliques.1 Publication

Developmental stagei

Up-regulated during leaf senescence.

Inductioni

By gibberellin, abscisic acid (ABA), heat shock and infection with the bacterial pathogen P.syringae. Not regulated by transition from dark to light.3 Publications

Gene expression databases

GenevisibleiQ8VZ56. AT.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi3702.AT4G25000.1.

Structurei

3D structure databases

ProteinModelPortaliQ8VZ56.
SMRiQ8VZ56.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 77Substrate bindingBy similarity2
Regioni201 – 206Substrate bindingBy similarity6
Regioni398 – 400Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.
HOGENOMiHOG000239525.
InParanoidiQ8VZ56.
KOiK01176.
OMAiGEQGYMP.
OrthoDBiEOG093600FS.
PhylomeDBiQ8VZ56.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VZ56-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSLHTLLFS SLLFFIVFPT FTFSSTLLFQ SFNWESWKKE GGFYNSLHNS
60 70 80 90 100
IDDIANAGIT HLWLPPPSQS VAPEGYLPGK LYDLNSSKYG SEAELKSLIK
110 120 130 140 150
ALNQKGIKAL ADIVINHRTA ERKDDKCGYC YFEGGTSDDR LDWDPSFVCR
160 170 180 190 200
NDPKFPGTGN LDTGGDFDGA PDIDHLNPRV QKELSEWMNW LKTEIGFHGW
210 220 230 240 250
RFDYVRGYAS SITKLYVQNT SPDFAVGEKW DDMKYGGDGK LDYDQNEHRS
260 270 280 290 300
GLKQWIEEAG GGVLTAFDFT TKGILQSAVK GELWRLKDSQ GKPPGMIGIM
310 320 330 340 350
PGNAVTFIDN HDTFRTWVFP SDKVLLGYVY ILTHPGTPCI FYNHYIEWGL
360 370 380 390 400
KESISKLVAI RNKNGIGSTS SVTIKAAEAD LYLAMIDDKV IMKIGPKQDV
410 420
GTLVPSNFAL AYSGLDFAVW EKK
Length:423
Mass (Da):47,378
Last modified:March 1, 2002 - v1
Checksum:i10D2119B6EA572EC
GO

Sequence cautioni

The sequence CAB36742 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB79409 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti419V → I in AAM64582 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035523 Genomic DNA. Translation: CAB36742.1. Sequence problems.
AL161562 Genomic DNA. Translation: CAB79409.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84990.1.
AY065233 mRNA. Translation: AAL38709.1.
AY117294 mRNA. Translation: AAM51369.1.
AY087021 mRNA. Translation: AAM64582.1.
PIRiT05521.
RefSeqiNP_567714.1. NM_118632.3.
UniGeneiAt.28556.

Genome annotation databases

EnsemblPlantsiAT4G25000.1; AT4G25000.1; AT4G25000.
GeneIDi828603.
GrameneiAT4G25000.1; AT4G25000.1; AT4G25000.
KEGGiath:AT4G25000.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035523 Genomic DNA. Translation: CAB36742.1. Sequence problems.
AL161562 Genomic DNA. Translation: CAB79409.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84990.1.
AY065233 mRNA. Translation: AAL38709.1.
AY117294 mRNA. Translation: AAM51369.1.
AY087021 mRNA. Translation: AAM64582.1.
PIRiT05521.
RefSeqiNP_567714.1. NM_118632.3.
UniGeneiAt.28556.

3D structure databases

ProteinModelPortaliQ8VZ56.
SMRiQ8VZ56.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G25000.1.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbiQ8VZ56.
ProMEXiQ8VZ56.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G25000.1; AT4G25000.1; AT4G25000.
GeneIDi828603.
GrameneiAT4G25000.1; AT4G25000.1; AT4G25000.
KEGGiath:AT4G25000.

Organism-specific databases

TAIRiAT4G25000.

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.
HOGENOMiHOG000239525.
InParanoidiQ8VZ56.
KOiK01176.
OMAiGEQGYMP.
OrthoDBiEOG093600FS.
PhylomeDBiQ8VZ56.

Enzyme and pathway databases

BioCyciARA:AT4G25000-MONOMER.

Miscellaneous databases

PROiQ8VZ56.

Gene expression databases

GenevisibleiQ8VZ56. AT.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMY1_ARATH
AccessioniPrimary (citable) accession number: Q8VZ56
Secondary accession number(s): Q8LBS5, Q9SW26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.