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Protein

Alpha-amylase 1

Gene

AMY1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Possesses alpha-amylase activity in vitro, but seems not required for breakdown of transitory starch in leaves.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.1 Publication

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi116Calcium 1By similarity1
Metal bindingi133Calcium 2By similarity1
Metal bindingi136Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi138Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi142Calcium 2By similarity1
Metal bindingi152Calcium 3By similarity1
Metal bindingi162Calcium 1By similarity1
Metal bindingi166Calcium 3By similarity1
Metal bindingi167Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi170Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi172Calcium 1By similarity1
Metal bindingi172Calcium 3By similarity1
Active sitei203NucleophileBy similarity1
Metal bindingi207Calcium 1; via carbonyl oxygenBy similarity1
Active sitei228Proton donorBy similarity1
Binding sitei230SubstrateBy similarity1
Binding sitei292SubstrateBy similarity1
Binding sitei311SubstrateBy similarity1
Sitei312Transition state stabilizerBy similarity1
Binding sitei393SubstrateBy similarity1
Binding sitei420SubstrateBy similarity1

GO - Molecular functioni

  • alpha-amylase activity Source: TAIR
  • alpha-amylase activity (releasing maltohexaose) Source: UniProtKB-EC
  • calcium ion binding Source: InterPro

GO - Biological processi

  • carbohydrate metabolic process Source: UniProtKB-KW
  • response to abscisic acid Source: TAIR
  • response to gibberellin Source: TAIR

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandMetal-binding

Enzyme and pathway databases

BioCyciARA:AT4G25000-MONOMER

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 1 (EC:3.2.1.11 Publication)
Short name:
AtAMY1
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:AMY1
Ordered Locus Names:At4g25000
ORF Names:F13M23.140
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G25000
TAIRilocus:2117398 AT4G25000

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Apoplast, Secreted

Pathology & Biotechi

Disruption phenotypei

Early flowering.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000041886125 – 423Alpha-amylase 1Add BLAST399

Proteomic databases

PaxDbiQ8VZ56
PRIDEiQ8VZ56
ProMEXiQ8VZ56

Expressioni

Tissue specificityi

Expressed in leaves, stems, flowers and developing siliques.1 Publication

Developmental stagei

Up-regulated during leaf senescence.

Inductioni

By gibberellin, abscisic acid (ABA), heat shock and infection with the bacterial pathogen P.syringae. Not regulated by transition from dark to light.3 Publications

Gene expression databases

ExpressionAtlasiQ8VZ56 baseline and differential
GenevisibleiQ8VZ56 AT

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi3702.AT4G25000.1

Structurei

3D structure databases

ProteinModelPortaliQ8VZ56
SMRiQ8VZ56
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 77Substrate bindingBy similarity2
Regioni201 – 206Substrate bindingBy similarity6
Regioni398 – 400Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0471 Eukaryota
COG0366 LUCA
HOGENOMiHOG000239525
InParanoidiQ8VZ56
KOiK01176
OMAiGEFWKDS
OrthoDBiEOG093600FS
PhylomeDBiQ8VZ56

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR012850 A-amylase_bs_C
IPR013775 A-amylase_pln
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF07821 Alpha-amyl_C2, 1 hit
PF00128 Alpha-amylase, 1 hit
PIRSFiPIRSF001028 Alph-amls_plant, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00810 Alpha-amyl_C2, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VZ56-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSLHTLLFS SLLFFIVFPT FTFSSTLLFQ SFNWESWKKE GGFYNSLHNS
60 70 80 90 100
IDDIANAGIT HLWLPPPSQS VAPEGYLPGK LYDLNSSKYG SEAELKSLIK
110 120 130 140 150
ALNQKGIKAL ADIVINHRTA ERKDDKCGYC YFEGGTSDDR LDWDPSFVCR
160 170 180 190 200
NDPKFPGTGN LDTGGDFDGA PDIDHLNPRV QKELSEWMNW LKTEIGFHGW
210 220 230 240 250
RFDYVRGYAS SITKLYVQNT SPDFAVGEKW DDMKYGGDGK LDYDQNEHRS
260 270 280 290 300
GLKQWIEEAG GGVLTAFDFT TKGILQSAVK GELWRLKDSQ GKPPGMIGIM
310 320 330 340 350
PGNAVTFIDN HDTFRTWVFP SDKVLLGYVY ILTHPGTPCI FYNHYIEWGL
360 370 380 390 400
KESISKLVAI RNKNGIGSTS SVTIKAAEAD LYLAMIDDKV IMKIGPKQDV
410 420
GTLVPSNFAL AYSGLDFAVW EKK
Length:423
Mass (Da):47,378
Last modified:March 1, 2002 - v1
Checksum:i10D2119B6EA572EC
GO

Sequence cautioni

The sequence CAB36742 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB79409 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti419V → I in AAM64582 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035523 Genomic DNA Translation: CAB36742.1 Sequence problems.
AL161562 Genomic DNA Translation: CAB79409.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE84990.1
AY065233 mRNA Translation: AAL38709.1
AY117294 mRNA Translation: AAM51369.1
AY087021 mRNA Translation: AAM64582.1
PIRiT05521
RefSeqiNP_567714.1, NM_118632.3
UniGeneiAt.28556

Genome annotation databases

EnsemblPlantsiAT4G25000.1; AT4G25000.1; AT4G25000
GeneIDi828603
GrameneiAT4G25000.1; AT4G25000.1; AT4G25000
KEGGiath:AT4G25000

Similar proteinsi

Entry informationi

Entry nameiAMY1_ARATH
AccessioniPrimary (citable) accession number: Q8VZ56
Secondary accession number(s): Q8LBS5, Q9SW26
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: March 1, 2002
Last modified: May 23, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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