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Protein

U-box domain-containing protein 14

Gene

PUB14

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as an E3 ubiquitin ligase with specific E2 ubiquitin-conjugating enzymes. Undergoes auto-ubiquitination.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei256 – 2561Putative E2 binding site
Sitei281 – 2811Putative E2 binding site
Sitei290 – 2901Putative E2 binding site

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • transmembrane receptor protein serine/threonine kinase binding Source: UniProtKB
  • ubiquitin-protein transferase activity Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciARA:GQT-2833-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
U-box domain-containing protein 14 (EC:6.3.2.-)
Alternative name(s):
E3 ubiquitin-protein ligase PUB14
Plant U-box protein 14
Prototypical U-box domain protein 14
Gene namesi
Name:PUB14
Ordered Locus Names:At3g54850
ORF Names:F28P10.170
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G54850.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 632632U-box domain-containing protein 14PRO_0000097097Add
BLAST

Proteomic databases

PaxDbiQ8VZ40.
PRIDEiQ8VZ40.

Expressioni

Tissue specificityi

Expressed in flowers, green siliques, seeds and rosette leaves.1 Publication

Gene expression databases

GenevisibleiQ8VZ40. AT.

Interactioni

Subunit structurei

Homodimer (Probable). Interacts with SNL1. Binds to SD11, SD16, SD17, SD18, SD113, SD129 and SD25.Curated2 Publications

GO - Molecular functioni

  • transmembrane receptor protein serine/threonine kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi9966. 14 interactions.
IntActiQ8VZ40. 14 interactions.
STRINGi3702.AT3G54850.1.

Structurei

Secondary structure

1
632
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi249 – 2535Combined sources
Turni255 – 2573Combined sources
Beta strandi262 – 2676Combined sources
Turni268 – 2703Combined sources
Beta strandi271 – 2744Combined sources
Helixi275 – 2828Combined sources
Turni283 – 2853Combined sources
Turni290 – 2923Combined sources
Turni305 – 3073Combined sources
Helixi308 – 31710Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T1HNMR-A249-321[»]
ProteinModelPortaliQ8VZ40.
SMRiQ8VZ40. Positions 245-321, 350-621.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8VZ40.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini247 – 32175U-boxAdd
BLAST
Repeati377 – 41640ARM 1Add
BLAST
Repeati418 – 45740ARM 2Add
BLAST
Repeati459 – 49840ARM 3Add
BLAST
Repeati500 – 53940ARM 4Add
BLAST
Repeati541 – 58040ARM 5Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi337 – 3404Poly-Ser

Domaini

The U-box N-terminal domain (UND) is not required for in vitro ubiquitination activity.

Sequence similaritiesi

Contains 5 ARM repeats.PROSITE-ProRule annotation
Contains 1 U-box domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0167. Eukaryota.
ENOG410XRTN. LUCA.
HOGENOMiHOG000238412.
InParanoidiQ8VZ40.
OMAiDSHAIPK.
OrthoDBiEOG093607HO.
PhylomeDBiQ8VZ40.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00514. Arm. 3 hits.
PF04564. U-box. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 5 hits.
SM00504. Ubox. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 2 hits.
PS51698. U_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VZ40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLTNCCSHE ELMSRLVDSV KEISGFSSSR GFIGKIQGDL VRRITLLSPF
60 70 80 90 100
FEELIDVNVE LKKDQITGFE AMRIALDSSL ELFRSVNGGS KLFQLFDRDS
110 120 130 140 150
LVEKFRDMTV EIEAALSQIP YEKIEVSEEV REQVQLLHFQ FKRAKERWEE
160 170 180 190 200
SDLQLSHDLA MAENVMDPDP IILKRLSQEL QLTTIDELKK ESHAIHEYFL
210 220 230 240 250
SYDGDPDDCF ERMSSLLKNL VDFVTMESSD PDPSTGSRIV SRHRSPVIPE
260 270 280 290 300
YFRCPISLEL MKDPVIVSTG QTYERSSIQK WLDAGHKTCP KSQETLLHAG
310 320 330 340 350
LTPNYVLKSL IALWCESNGI ELPQNQGSCR TTKIGGSSSS DCDRTFVLSL
360 370 380 390 400
LEKLANGTTE QQRAAAGELR LLAKRNVDNR VCIAEAGAIP LLVELLSSPD
410 420 430 440 450
PRTQEHSVTA LLNLSINEGN KGAIVDAGAI TDIVEVLKNG SMEARENAAA
460 470 480 490 500
TLFSLSVIDE NKVAIGAAGA IQALISLLEE GTRRGKKDAA TAIFNLCIYQ
510 520 530 540 550
GNKSRAVKGG IVDPLTRLLK DAGGGMVDEA LAILAILSTN QEGKTAIAEA
560 570 580 590 600
ESIPVLVEII RTGSPRNREN AAAILWYLCI GNIERLNVAR EVGADVALKE
610 620 630
LTENGTDRAK RKAASLLELI QQTEGVAVTT VP
Length:632
Mass (Da):69,397
Last modified:March 1, 2002 - v1
Checksum:iB9D82E373B9A6619
GO

Sequence cautioni

The sequence CAB41099 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049655 Genomic DNA. Translation: CAB41099.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79300.1.
AY065279 mRNA. Translation: AAL38755.1.
AY096530 mRNA. Translation: AAM20180.1.
PIRiT06735.
RefSeqiNP_191045.2. NM_115342.4.
UniGeneiAt.28098.

Genome annotation databases

EnsemblPlantsiAT3G54850.1; AT3G54850.1; AT3G54850.
GeneIDi824650.
GrameneiAT3G54850.1; AT3G54850.1; AT3G54850.
KEGGiath:AT3G54850.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049655 Genomic DNA. Translation: CAB41099.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79300.1.
AY065279 mRNA. Translation: AAL38755.1.
AY096530 mRNA. Translation: AAM20180.1.
PIRiT06735.
RefSeqiNP_191045.2. NM_115342.4.
UniGeneiAt.28098.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T1HNMR-A249-321[»]
ProteinModelPortaliQ8VZ40.
SMRiQ8VZ40. Positions 245-321, 350-621.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9966. 14 interactions.
IntActiQ8VZ40. 14 interactions.
STRINGi3702.AT3G54850.1.

Proteomic databases

PaxDbiQ8VZ40.
PRIDEiQ8VZ40.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G54850.1; AT3G54850.1; AT3G54850.
GeneIDi824650.
GrameneiAT3G54850.1; AT3G54850.1; AT3G54850.
KEGGiath:AT3G54850.

Organism-specific databases

TAIRiAT3G54850.

Phylogenomic databases

eggNOGiKOG0167. Eukaryota.
ENOG410XRTN. LUCA.
HOGENOMiHOG000238412.
InParanoidiQ8VZ40.
OMAiDSHAIPK.
OrthoDBiEOG093607HO.
PhylomeDBiQ8VZ40.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciARA:GQT-2833-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ8VZ40.
PROiQ8VZ40.

Gene expression databases

GenevisibleiQ8VZ40. AT.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00514. Arm. 3 hits.
PF04564. U-box. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 5 hits.
SM00504. Ubox. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 2 hits.
PS51698. U_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUB14_ARATH
AccessioniPrimary (citable) accession number: Q8VZ40
Secondary accession number(s): Q9SV34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 1, 2002
Last modified: September 7, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.