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Protein

Purple acid phosphatase 8

Gene

PAP8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi52IronBy similarity1
Metal bindingi85IronBy similarity1
Metal bindingi85ZincBy similarity1
Metal bindingi88IronBy similarity1
Metal bindingi123ZincBy similarity1
Metal bindingi217ZincBy similarity1
Active sitei226Proton donorBy similarity1
Metal bindingi252ZincBy similarity1
Metal bindingi254IronBy similarity1

GO - Molecular functioni

  • acid phosphatase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • dephosphorylation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT2G01890-MONOMER.
ReactomeiR-ATH-196843. Vitamin B2 (riboflavin) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 8 (EC:3.1.3.2)
Gene namesi
Name:PAP8
Ordered Locus Names:At2g01890
ORF Names:T23K3.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G01890.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000037281331 – 335Purple acid phosphatase 8Add BLAST305

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi60N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8VYZ2.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Gene expression databases

GenevisibleiQ8VYZ2. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi123. 1 interactor.
STRINGi3702.AT2G01890.1.

Structurei

3D structure databases

ProteinModelPortaliQ8VYZ2.
SMRiQ8VYZ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni252 – 254Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2679. Eukaryota.
COG1409. LUCA.
HOGENOMiHOG000239892.
InParanoidiQ8VYZ2.
OMAiSINSGIQ.
OrthoDBiEOG09360IST.
PhylomeDBiQ8VYZ2.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VYZ2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSLRDVKPI KLIFSIFCLV IILSACNSTA ELPRFVQPPE PDGSLSFLVV
60 70 80 90 100
GDWGRRGSYN QSQVALQMGK IGKDLNIDFL ISTGDNFYDD GIISPYDSQF
110 120 130 140 150
QDSFTNIYTA TSLQKPWYNV LGNHDYRGNV YAQLSPILRD LDCRWICLRS
160 170 180 190 200
YVVNAEIVDI FFVDTTPFVD RYFDEPKDHV YDWRGVLPRN KYLNSLLTDV
210 220 230 240 250
DVALQESMAK WKIVVGHHTI KSAGHHGNTI ELEKQLLPIL EANEVDLYIN
260 270 280 290 300
GHDHCLEHIS SINSGIQFMT SGGGSKAWKG DVNDWNPQEM RFYYDGQGFM
310 320 330
SVYTSEAELR VVFYDGLGHV LHRWSTLKNG VYSDI
Length:335
Mass (Da):38,173
Last modified:March 1, 2002 - v1
Checksum:i4202286D73CBFF7C
GO
Isoform 2 (identifier: Q8VYZ2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     128-155: Missing.

Note: No experimental confirmation available.
Show »
Length:307
Mass (Da):34,952
Checksum:i2EBFA76DB858ED35
GO

Sequence cautioni

The sequence AAD21785 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAF19823 differs from that shown. Reason: Frameshift at positions 293, 304 and 310.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti261S → G in AAF19823 (PubMed:10854785).Curated1
Sequence conflicti266I → M in AAF19823 (PubMed:10854785).Curated1
Sequence conflicti275S → F in AAF19823 (PubMed:10854785).Curated1
Sequence conflicti287P → L in AAF19823 (PubMed:10854785).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_037190128 – 155Missing in isoform 2. CuratedAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492660 mRNA. Translation: AAM15909.1.
AC007069 Genomic DNA. Translation: AAD21785.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC05512.1.
CP002685 Genomic DNA. Translation: AEC05513.1.
AY065434 mRNA. Translation: AAL38875.1.
AY117232 mRNA. Translation: AAM51307.1.
AF200827 mRNA. Translation: AAF19823.1. Frameshift.
PIRiD84430.
RefSeqiNP_178298.2. NM_126250.5. [Q8VYZ2-1]
NP_973397.1. NM_201668.2. [Q8VYZ2-2]
UniGeneiAt.16914.

Genome annotation databases

EnsemblPlantsiAT2G01890.1; AT2G01890.1; AT2G01890. [Q8VYZ2-1]
GeneIDi814720.
GrameneiAT2G01890.1; AT2G01890.1; AT2G01890.
KEGGiath:AT2G01890.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492660 mRNA. Translation: AAM15909.1.
AC007069 Genomic DNA. Translation: AAD21785.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC05512.1.
CP002685 Genomic DNA. Translation: AEC05513.1.
AY065434 mRNA. Translation: AAL38875.1.
AY117232 mRNA. Translation: AAM51307.1.
AF200827 mRNA. Translation: AAF19823.1. Frameshift.
PIRiD84430.
RefSeqiNP_178298.2. NM_126250.5. [Q8VYZ2-1]
NP_973397.1. NM_201668.2. [Q8VYZ2-2]
UniGeneiAt.16914.

3D structure databases

ProteinModelPortaliQ8VYZ2.
SMRiQ8VYZ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123. 1 interactor.
STRINGi3702.AT2G01890.1.

Proteomic databases

PaxDbiQ8VYZ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G01890.1; AT2G01890.1; AT2G01890. [Q8VYZ2-1]
GeneIDi814720.
GrameneiAT2G01890.1; AT2G01890.1; AT2G01890.
KEGGiath:AT2G01890.

Organism-specific databases

TAIRiAT2G01890.

Phylogenomic databases

eggNOGiKOG2679. Eukaryota.
COG1409. LUCA.
HOGENOMiHOG000239892.
InParanoidiQ8VYZ2.
OMAiSINSGIQ.
OrthoDBiEOG09360IST.
PhylomeDBiQ8VYZ2.

Enzyme and pathway databases

BioCyciARA:AT2G01890-MONOMER.
ReactomeiR-ATH-196843. Vitamin B2 (riboflavin) metabolism.

Miscellaneous databases

PROiQ8VYZ2.

Gene expression databases

GenevisibleiQ8VYZ2. AT.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPA8_ARATH
AccessioniPrimary (citable) accession number: Q8VYZ2
Secondary accession number(s): Q3EC86, Q9SDZ8, Q9SIS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.