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Q8VYZ2 (PPA8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Purple acid phosphatase 8

EC=3.1.3.2
Gene names
Name:PAP8
Ordered Locus Names:At2g01890
ORF Names:T23K3.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Secreted By similarity.

Tissue specificity

Expressed in roots, stems, leaves, flowers and siliques. Ref.6

Sequence similarities

Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Sequence caution

The sequence AAD21785.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAF19823.1 differs from that shown. Reason: Frameshift at positions 293, 304 and 310.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   LigandIron
Metal-binding
Zinc
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdephosphorylation

Inferred from sequence or structural similarity Ref.5. Source: TAIR

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionacid phosphatase activity

Inferred from sequence or structural similarity Ref.1Ref.6. Source: TAIR

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8VYZ2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8VYZ2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     128-155: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Potential
Chain31 – 335305Purple acid phosphatase 8
PRO_0000372813

Regions

Region252 – 2543Substrate binding By similarity

Sites

Active site2261Proton donor By similarity
Metal binding521Iron By similarity
Metal binding851Iron By similarity
Metal binding851Zinc By similarity
Metal binding881Iron By similarity
Metal binding1231Zinc By similarity
Metal binding2171Zinc By similarity
Metal binding2521Zinc By similarity
Metal binding2541Iron By similarity

Amino acid modifications

Glycosylation601N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence128 – 15528Missing in isoform 2.
VSP_037190

Experimental info

Sequence conflict2611S → G in AAF19823. Ref.5
Sequence conflict2661I → M in AAF19823. Ref.5
Sequence conflict2751S → F in AAF19823. Ref.5
Sequence conflict2871P → L in AAF19823. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 4202286D73CBFF7C

FASTA33538,173
        10         20         30         40         50         60 
MDSLRDVKPI KLIFSIFCLV IILSACNSTA ELPRFVQPPE PDGSLSFLVV GDWGRRGSYN 

        70         80         90        100        110        120 
QSQVALQMGK IGKDLNIDFL ISTGDNFYDD GIISPYDSQF QDSFTNIYTA TSLQKPWYNV 

       130        140        150        160        170        180 
LGNHDYRGNV YAQLSPILRD LDCRWICLRS YVVNAEIVDI FFVDTTPFVD RYFDEPKDHV 

       190        200        210        220        230        240 
YDWRGVLPRN KYLNSLLTDV DVALQESMAK WKIVVGHHTI KSAGHHGNTI ELEKQLLPIL 

       250        260        270        280        290        300 
EANEVDLYIN GHDHCLEHIS SINSGIQFMT SGGGSKAWKG DVNDWNPQEM RFYYDGQGFM 

       310        320        330 
SVYTSEAELR VVFYDGLGHV LHRWSTLKNG VYSDI 

« Hide

Isoform 2 [UniParc].

Checksum: 2EBFA76DB858ED35
Show »

FASTA30734,952

References

« Hide 'large scale' references
[1]"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, NOMENCLATURE.
Strain: cv. Col-1.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2).
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Identification of mammalian-like purple acid phosphatases in a wide range of plants."
Schenk G., Guddat L.W., Ge Y., Carrington L.E., Hume D.A., Hamilton S., de Jersey J.
Gene 250:117-125(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-314 (ISOFORM 1).
Strain: cv. Columbia.
[6]"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF492660 mRNA. Translation: AAM15909.1.
AC007069 Genomic DNA. Translation: AAD21785.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC05512.1.
CP002685 Genomic DNA. Translation: AEC05513.1.
AY065434 mRNA. Translation: AAL38875.1.
AY117232 mRNA. Translation: AAM51307.1.
AF200827 mRNA. Translation: AAF19823.1. Frameshift.
PIRD84430.
RefSeqNP_178298.2. NM_126250.4.
NP_973397.1. NM_201668.2.
UniGeneAt.16914.

3D structure databases

ProteinModelPortalQ8VYZ2.
SMRQ8VYZ2. Positions 41-323.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ8VYZ2.
PRIDEQ8VYZ2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G01890.1; AT2G01890.1; AT2G01890. [Q8VYZ2-1]
GeneID814720.
KEGGath:AT2G01890.

Organism-specific databases

TAIRAT2G01890.

Phylogenomic databases

eggNOGCOG1409.
HOGENOMHOG000239892.
InParanoidQ8VYZ2.
OMASINSGIQ.
PhylomeDBQ8VYZ2.
ProtClustDBCLSN2688499.

Enzyme and pathway databases

BioCycARA:AT2G01890-MONOMER.
ARA:GQT-1995-MONOMER.

Gene expression databases

GenevestigatorQ8VYZ2.

Family and domain databases

InterProIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_apaH.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFPIRSF000898. Acid_Ptase_5. 1 hit.
ProtoNetSearch...

Other

PROQ8VYZ2.

Entry information

Entry namePPA8_ARATH
AccessionPrimary (citable) accession number: Q8VYZ2
Secondary accession number(s): Q3EC86, Q9SDZ8, Q9SIS5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2002
Last modified: April 16, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names