Q8VYZ2 (PPA8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Purple acid phosphatase 8
EC=3.1.3.2

Gene names

Name:	PAP8
Ordered Locus Names:	At2g01890
ORF Names:	T23K3.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed in roots, stems, leaves, flowers and siliques. Ref.6
Sequence similarities	Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.
Sequence caution	The sequence AAD21785.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAF19823.1 differs from that shown. Reason: Frameshift at positions 293, 304 and 310.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acid phosphatase activity Inferred from sequence or structural similarity Ref.1Ref.6. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8VYZ2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8VYZ2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     128-155: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 335	305	Purple acid phosphatase 8		PRO_0000372813
Regions
	Region	252 – 254	3	Substrate binding By similarity
Sites
	Active site	226	1	Proton donor By similarity
	Metal binding	52	1	Iron By similarity
	Metal binding	85	1	Iron By similarity
	Metal binding	85	1	Zinc By similarity
	Metal binding	88	1	Iron By similarity
	Metal binding	123	1	Zinc By similarity
	Metal binding	217	1	Zinc By similarity
	Metal binding	252	1	Zinc By similarity
	Metal binding	254	1	Iron By similarity
Amino acid modifications
	Glycosylation	60	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	128 – 155	28	Missing in isoform 2.		VSP_037190
Experimental info
	Sequence conflict	261	1	S → G in AAF19823. Ref.5
	Sequence conflict	266	1	I → M in AAF19823. Ref.5
	Sequence conflict	275	1	S → F in AAF19823. Ref.5
	Sequence conflict	287	1	P → L in AAF19823. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 4202286D73CBFF7C	FASTA	335	38,173
	10 20 30 40 50 60 MDSLRDVKPI KLIFSIFCLV IILSACNSTA ELPRFVQPPE PDGSLSFLVV GDWGRRGSYN 70 80 90 100 110 120 QSQVALQMGK IGKDLNIDFL ISTGDNFYDD GIISPYDSQF QDSFTNIYTA TSLQKPWYNV 130 140 150 160 170 180 LGNHDYRGNV YAQLSPILRD LDCRWICLRS YVVNAEIVDI FFVDTTPFVD RYFDEPKDHV 190 200 210 220 230 240 YDWRGVLPRN KYLNSLLTDV DVALQESMAK WKIVVGHHTI KSAGHHGNTI ELEKQLLPIL 250 260 270 280 290 300 EANEVDLYIN GHDHCLEHIS SINSGIQFMT SGGGSKAWKG DVNDWNPQEM RFYYDGQGFM 310 320 330 SVYTSEAELR VVFYDGLGHV LHRWSTLKNG VYSDI
	Isoform 2 [UniParc]. Checksum: 2EBFA76DB858ED35	FASTA	307	34,952
	10 20 30 40 50 60 MDSLRDVKPI KLIFSIFCLV IILSACNSTA ELPRFVQPPE PDGSLSFLVV GDWGRRGSYN 70 80 90 100 110 120 QSQVALQMGK IGKDLNIDFL ISTGDNFYDD GIISPYDSQF QDSFTNIYTA TSLQKPWYNV 130 140 150 160 170 180 LGNHDYREIV DIFFVDTTPF VDRYFDEPKD HVYDWRGVLP RNKYLNSLLT DVDVALQESM 190 200 210 220 230 240 AKWKIVVGHH TIKSAGHHGN TIELEKQLLP ILEANEVDLY INGHDHCLEH ISSINSGIQF 250 260 270 280 290 300 MTSGGGSKAW KGDVNDWNPQ EMRFYYDGQG FMSVYTSEAE LRVVFYDGLG HVLHRWSTLK NGVYSDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation." Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D. J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, NOMENCLATURE. Strain: cv. Col-1.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2). Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Identification of mammalian-like purple acid phosphatases in a wide range of plants." Schenk G., Guddat L.W., Ge Y., Carrington L.E., Hume D.A., Hamilton S., de Jersey J. Gene 250:117-125(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-314 (ISOFORM 1). Strain: cv. Columbia.
[6]	"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower." Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D. Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF492660 mRNA. Translation: AAM15909.1. AC007069 Genomic DNA. Translation: AAD21785.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC05512.1. CP002685 Genomic DNA. Translation: AEC05513.1. AY065434 mRNA. Translation: AAL38875.1. AY117232 mRNA. Translation: AAM51307.1. AF200827 mRNA. Translation: AAF19823.1. Frameshift.
IPI	IPI00517804. IPI00519896.
PIR	D84430.
RefSeq	NP_178298.2. NM_126250.4. NP_973397.1. NM_201668.2.
UniGene	At.16914.
3D structure databases
HSSP	HSSP built from PDB template 1QFC based on UniProtKB P29288.
ProteinModelPortal	Q8VYZ2.
SMR	Q8VYZ2. Positions 41-323.
ModBase	Search...
Proteomic databases
PaxDb	Q8VYZ2.
PRIDE	Q8VYZ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G01890.1; AT2G01890.1; AT2G01890.
GeneID	814720.
KEGG	ath:AT2G01890.
Organism-specific databases
TAIR	At2g01890.
Phylogenomic databases
eggNOG	COG1409.
HOGENOM	HOG000239892.
InParanoid	Q8VYZ2.
OMA	SINSGIQ.
PhylomeDB	Q8VYZ2.
ProtClustDB	CLSN2688499.
Gene expression databases
Genevestigator	Q8VYZ2.
Family and domain databases
InterPro	IPR024927. Acid_Pase_5. IPR004843. Metallo_PEstase_dom. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF000898. Acid_Ptase_5. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPA8_ARATH

Accession

Primary (citable) accession number: Q8VYZ2
Secondary accession number(s): Q3EC86, Q9SDZ8, Q9SIS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program