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Q8VYZ2

- PPA8_ARATH

UniProt

Q8VYZ2 - PPA8_ARATH

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Protein

Purple acid phosphatase 8

Gene

PAP8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Binds 1 iron ion per subunit.By similarity
Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi52 – 521IronBy similarity
Metal bindingi85 – 851IronBy similarity
Metal bindingi85 – 851ZincBy similarity
Metal bindingi88 – 881IronBy similarity
Metal bindingi123 – 1231ZincBy similarity
Metal bindingi217 – 2171ZincBy similarity
Active sitei226 – 2261Proton donorBy similarity
Metal bindingi252 – 2521ZincBy similarity
Metal bindingi254 – 2541IronBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: TAIR
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dephosphorylation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT2G01890-MONOMER.
ARA:GQT-1995-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 8 (EC:3.1.3.2)
Gene namesi
Name:PAP8
Ordered Locus Names:At2g01890
ORF Names:T23K3.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G01890.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence AnalysisAdd
BLAST
Chaini31 – 335305Purple acid phosphatase 8PRO_0000372813Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi60 – 601N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8VYZ2.
PRIDEiQ8VYZ2.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Gene expression databases

GenevestigatoriQ8VYZ2.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8VYZ2.
SMRiQ8VYZ2. Positions 41-323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni252 – 2543Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000239892.
InParanoidiQ8VYZ2.
OMAiKYDWRGV.
PhylomeDBiQ8VYZ2.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8VYZ2-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSLRDVKPI KLIFSIFCLV IILSACNSTA ELPRFVQPPE PDGSLSFLVV
60 70 80 90 100
GDWGRRGSYN QSQVALQMGK IGKDLNIDFL ISTGDNFYDD GIISPYDSQF
110 120 130 140 150
QDSFTNIYTA TSLQKPWYNV LGNHDYRGNV YAQLSPILRD LDCRWICLRS
160 170 180 190 200
YVVNAEIVDI FFVDTTPFVD RYFDEPKDHV YDWRGVLPRN KYLNSLLTDV
210 220 230 240 250
DVALQESMAK WKIVVGHHTI KSAGHHGNTI ELEKQLLPIL EANEVDLYIN
260 270 280 290 300
GHDHCLEHIS SINSGIQFMT SGGGSKAWKG DVNDWNPQEM RFYYDGQGFM
310 320 330
SVYTSEAELR VVFYDGLGHV LHRWSTLKNG VYSDI
Length:335
Mass (Da):38,173
Last modified:March 1, 2002 - v1
Checksum:i4202286D73CBFF7C
GO
Isoform 2 (identifier: Q8VYZ2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     128-155: Missing.

Note: No experimental confirmation available.

Show »
Length:307
Mass (Da):34,952
Checksum:i2EBFA76DB858ED35
GO

Sequence cautioni

The sequence AAF19823.1 differs from that shown. Reason: Frameshift at positions 293, 304 and 310.
The sequence AAD21785.1 differs from that shown. Reason: Erroneous gene model prediction.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti261 – 2611S → G in AAF19823. (PubMed:10854785)Curated
Sequence conflicti266 – 2661I → M in AAF19823. (PubMed:10854785)Curated
Sequence conflicti275 – 2751S → F in AAF19823. (PubMed:10854785)Curated
Sequence conflicti287 – 2871P → L in AAF19823. (PubMed:10854785)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei128 – 15528Missing in isoform 2. CuratedVSP_037190Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF492660 mRNA. Translation: AAM15909.1.
AC007069 Genomic DNA. Translation: AAD21785.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC05512.1.
CP002685 Genomic DNA. Translation: AEC05513.1.
AY065434 mRNA. Translation: AAL38875.1.
AY117232 mRNA. Translation: AAM51307.1.
AF200827 mRNA. Translation: AAF19823.1. Frameshift.
PIRiD84430.
RefSeqiNP_178298.2. NM_126250.4. [Q8VYZ2-1]
NP_973397.1. NM_201668.2. [Q8VYZ2-2]
UniGeneiAt.16914.

Genome annotation databases

EnsemblPlantsiAT2G01890.1; AT2G01890.1; AT2G01890. [Q8VYZ2-1]
GeneIDi814720.
KEGGiath:AT2G01890.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF492660 mRNA. Translation: AAM15909.1 .
AC007069 Genomic DNA. Translation: AAD21785.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC05512.1 .
CP002685 Genomic DNA. Translation: AEC05513.1 .
AY065434 mRNA. Translation: AAL38875.1 .
AY117232 mRNA. Translation: AAM51307.1 .
AF200827 mRNA. Translation: AAF19823.1 . Frameshift.
PIRi D84430.
RefSeqi NP_178298.2. NM_126250.4. [Q8VYZ2-1 ]
NP_973397.1. NM_201668.2. [Q8VYZ2-2 ]
UniGenei At.16914.

3D structure databases

ProteinModelPortali Q8VYZ2.
SMRi Q8VYZ2. Positions 41-323.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q8VYZ2.
PRIDEi Q8VYZ2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G01890.1 ; AT2G01890.1 ; AT2G01890 . [Q8VYZ2-1 ]
GeneIDi 814720.
KEGGi ath:AT2G01890.

Organism-specific databases

TAIRi AT2G01890.

Phylogenomic databases

eggNOGi COG1409.
HOGENOMi HOG000239892.
InParanoidi Q8VYZ2.
OMAi KYDWRGV.
PhylomeDBi Q8VYZ2.

Enzyme and pathway databases

BioCyci ARA:AT2G01890-MONOMER.
ARA:GQT-1995-MONOMER.

Miscellaneous databases

PROi Q8VYZ2.

Gene expression databases

Genevestigatori Q8VYZ2.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PIRSFi PIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMi SSF56300. SSF56300. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
    Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
    J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, NOMENCLATURE.
    Strain: cv. Col-1.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2).
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Identification of mammalian-like purple acid phosphatases in a wide range of plants."
    Schenk G., Guddat L.W., Ge Y., Carrington L.E., Hume D.A., Hamilton S., de Jersey J.
    Gene 250:117-125(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-314 (ISOFORM 1).
    Strain: cv. Columbia.
  6. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
    Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
    Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiPPA8_ARATH
AccessioniPrimary (citable) accession number: Q8VYZ2
Secondary accession number(s): Q3EC86, Q9SDZ8, Q9SIS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2002
Last modified: October 29, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3