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Q8VYZ2

- PPA8_ARATH

UniProt

Q8VYZ2 - PPA8_ARATH

Protein

Purple acid phosphatase 8

Gene

PAP8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.

    Cofactori

    Binds 1 iron ion per subunit.By similarity
    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi52 – 521IronBy similarity
    Metal bindingi85 – 851IronBy similarity
    Metal bindingi85 – 851ZincBy similarity
    Metal bindingi88 – 881IronBy similarity
    Metal bindingi123 – 1231ZincBy similarity
    Metal bindingi217 – 2171ZincBy similarity
    Active sitei226 – 2261Proton donorBy similarity
    Metal bindingi252 – 2521ZincBy similarity
    Metal bindingi254 – 2541IronBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: TAIR
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. dephosphorylation Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Iron, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT2G01890-MONOMER.
    ARA:GQT-1995-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Purple acid phosphatase 8 (EC:3.1.3.2)
    Gene namesi
    Name:PAP8
    Ordered Locus Names:At2g01890
    ORF Names:T23K3.8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G01890.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3030Sequence AnalysisAdd
    BLAST
    Chaini31 – 335305Purple acid phosphatase 8PRO_0000372813Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi60 – 601N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8VYZ2.
    PRIDEiQ8VYZ2.

    Expressioni

    Tissue specificityi

    Expressed in roots, stems, leaves, flowers and siliques.1 Publication

    Gene expression databases

    GenevestigatoriQ8VYZ2.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VYZ2.
    SMRiQ8VYZ2. Positions 41-323.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni252 – 2543Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1409.
    HOGENOMiHOG000239892.
    InParanoidiQ8VYZ2.
    OMAiKYDWRGV.
    PhylomeDBiQ8VYZ2.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR024927. Acid_Pase_5.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000898. Acid_Ptase_5. 1 hit.
    SUPFAMiSSF56300. SSF56300. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8VYZ2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDSLRDVKPI KLIFSIFCLV IILSACNSTA ELPRFVQPPE PDGSLSFLVV    50
    GDWGRRGSYN QSQVALQMGK IGKDLNIDFL ISTGDNFYDD GIISPYDSQF 100
    QDSFTNIYTA TSLQKPWYNV LGNHDYRGNV YAQLSPILRD LDCRWICLRS 150
    YVVNAEIVDI FFVDTTPFVD RYFDEPKDHV YDWRGVLPRN KYLNSLLTDV 200
    DVALQESMAK WKIVVGHHTI KSAGHHGNTI ELEKQLLPIL EANEVDLYIN 250
    GHDHCLEHIS SINSGIQFMT SGGGSKAWKG DVNDWNPQEM RFYYDGQGFM 300
    SVYTSEAELR VVFYDGLGHV LHRWSTLKNG VYSDI 335
    Length:335
    Mass (Da):38,173
    Last modified:March 1, 2002 - v1
    Checksum:i4202286D73CBFF7C
    GO
    Isoform 2 (identifier: Q8VYZ2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         128-155: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:307
    Mass (Da):34,952
    Checksum:i2EBFA76DB858ED35
    GO

    Sequence cautioni

    The sequence AAF19823.1 differs from that shown. Reason: Frameshift at positions 293, 304 and 310.
    The sequence AAD21785.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti261 – 2611S → G in AAF19823. (PubMed:10854785)Curated
    Sequence conflicti266 – 2661I → M in AAF19823. (PubMed:10854785)Curated
    Sequence conflicti275 – 2751S → F in AAF19823. (PubMed:10854785)Curated
    Sequence conflicti287 – 2871P → L in AAF19823. (PubMed:10854785)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei128 – 15528Missing in isoform 2. CuratedVSP_037190Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF492660 mRNA. Translation: AAM15909.1.
    AC007069 Genomic DNA. Translation: AAD21785.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC05512.1.
    CP002685 Genomic DNA. Translation: AEC05513.1.
    AY065434 mRNA. Translation: AAL38875.1.
    AY117232 mRNA. Translation: AAM51307.1.
    AF200827 mRNA. Translation: AAF19823.1. Frameshift.
    PIRiD84430.
    RefSeqiNP_178298.2. NM_126250.4. [Q8VYZ2-1]
    NP_973397.1. NM_201668.2. [Q8VYZ2-2]
    UniGeneiAt.16914.

    Genome annotation databases

    EnsemblPlantsiAT2G01890.1; AT2G01890.1; AT2G01890. [Q8VYZ2-1]
    GeneIDi814720.
    KEGGiath:AT2G01890.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF492660 mRNA. Translation: AAM15909.1 .
    AC007069 Genomic DNA. Translation: AAD21785.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC05512.1 .
    CP002685 Genomic DNA. Translation: AEC05513.1 .
    AY065434 mRNA. Translation: AAL38875.1 .
    AY117232 mRNA. Translation: AAM51307.1 .
    AF200827 mRNA. Translation: AAF19823.1 . Frameshift.
    PIRi D84430.
    RefSeqi NP_178298.2. NM_126250.4. [Q8VYZ2-1 ]
    NP_973397.1. NM_201668.2. [Q8VYZ2-2 ]
    UniGenei At.16914.

    3D structure databases

    ProteinModelPortali Q8VYZ2.
    SMRi Q8VYZ2. Positions 41-323.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q8VYZ2.
    PRIDEi Q8VYZ2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G01890.1 ; AT2G01890.1 ; AT2G01890 . [Q8VYZ2-1 ]
    GeneIDi 814720.
    KEGGi ath:AT2G01890.

    Organism-specific databases

    TAIRi AT2G01890.

    Phylogenomic databases

    eggNOGi COG1409.
    HOGENOMi HOG000239892.
    InParanoidi Q8VYZ2.
    OMAi KYDWRGV.
    PhylomeDBi Q8VYZ2.

    Enzyme and pathway databases

    BioCyci ARA:AT2G01890-MONOMER.
    ARA:GQT-1995-MONOMER.

    Miscellaneous databases

    PROi Q8VYZ2.

    Gene expression databases

    Genevestigatori Q8VYZ2.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR024927. Acid_Pase_5.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000898. Acid_Ptase_5. 1 hit.
    SUPFAMi SSF56300. SSF56300. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
      Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
      J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, NOMENCLATURE.
      Strain: cv. Col-1.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2).
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Identification of mammalian-like purple acid phosphatases in a wide range of plants."
      Schenk G., Guddat L.W., Ge Y., Carrington L.E., Hume D.A., Hamilton S., de Jersey J.
      Gene 250:117-125(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-314 (ISOFORM 1).
      Strain: cv. Columbia.
    6. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
      Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
      Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPPA8_ARATH
    AccessioniPrimary (citable) accession number: Q8VYZ2
    Secondary accession number(s): Q3EC86, Q9SDZ8, Q9SIS5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3