Q8VYW2 (BAM9_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inactive beta-amylase 9

Alternative name(s):
1,4-alpha-D-glucan maltohydrolase
Inactive beta-amylase 3

Gene names

Name:	BAM9
Synonyms:	BMY3
Ordered Locus Names:	At5g18670
ORF Names:	T1A4.50

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Cytoplasm Potential.
Tissue specificity	Mostly expressed in young floral buds, flowers and roots, and, to a later extent, in stems and leaves. Ref.1
Induction	Circadian-regulated, with a peak in expression just before the light period in short day conditions. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 14 family.
Caution	In contrast to other members of the family, lacks the conserved Glu active site in position 449, which is replaced by a Gln residue, suggesting it is inactive.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Cellular component	Cytoplasm
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-amylase activity Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 536	536	Inactive beta-amylase 9		PRO_0000393423
Experimental info
	Sequence conflict	69 – 70	2	DD → GA in AAK85300. Ref.1
	Sequence conflict	442 – 443	2	QG → RKA in AAK85300. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8VYW2 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: C21F0582BE72E190	FASTA	536	58,281
	10 20 30 40 50 60 MEVSVIGNPQ ARICRAELAY RELGFRFGSD VISGESRNRV SFCNQSSKWK EIAIRCSSRS 70 80 90 100 110 120 VKCEAIVSDD ASPFLKSTPK SKSLESVKLF VGLPLDTVSD CNNVNHLKAI TAGLKALKLL 130 140 150 160 170 180 GVEGIELPIF WGVVEKEAAG KYEWSGYLAV AEIVKKVGLK LHASLSFHGS KQTEIGLPDW 190 200 210 220 230 240 VAKIGDAEPG IYFTDRYGQQ YKDCLSFAVD DVPVLDGKTP MEVYRGFCES FKSAFADYMG 250 260 270 280 290 300 NTITGITLGL GPDGELKYPS HQHNAKLSGA GEFQCYDKHM LSALKGYAES TGNPLWGLGG 310 320 330 340 350 360 PHDAPAYDQQ PNSSSFFSDG GSWESQYGDF FLSWYSSLLT SHADRVLSVA SSAFSGIGVP 370 380 390 400 410 420 LCGKLPLLHQ WHKLRSHPSE LTAGFYSSNG QDRYEAIAEI FAKNSCRMII PGMDLSDEHQ 430 440 450 460 470 480 SPESLSSPES LLGHIKTSCK KQGVVVSGQN SSTPVPGGFE RIVENLKDEN VGIDLFTYQR 490 500 510 520 530 MGALFFSPEH FHAFTVFVRN LSQFELSSDD QASEAEVEAE TASIGSGTGA PSLQTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel putative beta-amylase gene and ATbeta-Amy from Arabidopsis thaliana are circadian regulated." Chandler J.W., Apel K., Melzer S. Plant Sci. 161:1019-1024(2001) Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Strain: cv. Landsberg erecta.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Beta-AMYLASE4, a noncatalytic protein required for starch breakdown, acts upstream of three active beta-amylases in Arabidopsis chloroplasts." Fulton D.C., Stettler M., Mettler T., Vaughan C.K., Li J., Francisco P., Gil M., Reinhold H., Eicke S., Messerli G., Dorken G., Halliday K., Smith A.M., Smith S.M., Zeeman S.C. Plant Cell 20:1040-1058(2008) [PubMed: 18390594] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF402598 mRNA. Translation: AAK85300.1. AC051627 Genomic DNA. No translation available. CP002688 Genomic DNA. Translation: AED92597.1. AY069879 mRNA. Translation: AAL47434.1. AY142007 mRNA. Translation: AAM98271.1. AY087036 mRNA. Translation: AAM64597.1.
IPI	IPI00528458.
RefSeq	NP_197368.1. NM_121872.2.
UniGene	At.19910.
3D structure databases
HSSP	HSSP built from PDB template 1FA2 based on UniProtKB P10537.
ProteinModelPortal	Q8VYW2.
SMR	Q8VYW2. Positions 83-524.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8VYW2.
Protein family/group databases
CAZy	GH14. Glycoside Hydrolase Family 14.
Proteomic databases
PRIDE	Q8VYW2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G18670.1; AT5G18670.1; AT5G18670.
GeneID	831985.
GenomeReviews	Gene locus AT5G18670 in contig BA000015_GR.
KEGG	ath:AT5G18670.
NMPDR	fig|3702.1.peg.24056.
Organism-specific databases
TAIR	At5g18670.
Phylogenomic databases
eggNOG	NOG77898.
GeneTree	EPGT00070000028196.
InParanoid	Q8VYW2.
OMA	GNPLWGL.
PhylomeDB	Q8VYW2.
ProtClustDB	CLSN2916428.
Gene expression databases
Genevestigator	Q8VYW2.
Family and domain databases
InterPro	IPR001554. Glyco_hydro_14. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF01373. Glyco_hydro_14. 1 hit. [Graphical view]
PRINTS	PR00750. BETAAMYLASE.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00506. BETA_AMYLASE_1. False negative. PS00679. BETA_AMYLASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BAM9_ARATH

Accession

Primary (citable) accession number: Q8VYW2
Secondary accession number(s): Q946D4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 20, 2010
Last sequence update:	March 1, 2002
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program