Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q8VYW2 (BAM9_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inactive beta-amylase 9
Alternative name(s):
1,4-alpha-D-glucan maltohydrolase
Inactive beta-amylase 3
Gene names
Name:BAM9
Synonyms:BMY3
Ordered Locus Names:At5g18670
ORF Names:T1A4.50
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length536 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Subcellular location

Cytoplasm Potential.

Tissue specificity

Mostly expressed in young floral buds, flowers and roots, and, to a later extent, in stems and leaves. Ref.1

Induction

Circadian-regulated, with a peak in expression just before the light period in short day conditions. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 14 family.

Caution

In contrast to other members of the family, lacks the conserved Glu active site in position 449, which is replaced by a Gln residue, suggesting it is inactive.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Polysaccharide degradation
   Cellular componentCytoplasm
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processpolysaccharide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionbeta-amylase activity

Inferred from electronic annotation. Source: InterPro

cation binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 536536Inactive beta-amylase 9
PRO_0000393423

Experimental info

Sequence conflict69 – 702DD → GA in AAK85300. Ref.1
Sequence conflict442 – 4432QG → RKA in AAK85300. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8VYW2 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: C21F0582BE72E190

FASTA53658,281
        10         20         30         40         50         60 
MEVSVIGNPQ ARICRAELAY RELGFRFGSD VISGESRNRV SFCNQSSKWK EIAIRCSSRS 

        70         80         90        100        110        120 
VKCEAIVSDD ASPFLKSTPK SKSLESVKLF VGLPLDTVSD CNNVNHLKAI TAGLKALKLL 

       130        140        150        160        170        180 
GVEGIELPIF WGVVEKEAAG KYEWSGYLAV AEIVKKVGLK LHASLSFHGS KQTEIGLPDW 

       190        200        210        220        230        240 
VAKIGDAEPG IYFTDRYGQQ YKDCLSFAVD DVPVLDGKTP MEVYRGFCES FKSAFADYMG 

       250        260        270        280        290        300 
NTITGITLGL GPDGELKYPS HQHNAKLSGA GEFQCYDKHM LSALKGYAES TGNPLWGLGG 

       310        320        330        340        350        360 
PHDAPAYDQQ PNSSSFFSDG GSWESQYGDF FLSWYSSLLT SHADRVLSVA SSAFSGIGVP 

       370        380        390        400        410        420 
LCGKLPLLHQ WHKLRSHPSE LTAGFYSSNG QDRYEAIAEI FAKNSCRMII PGMDLSDEHQ 

       430        440        450        460        470        480 
SPESLSSPES LLGHIKTSCK KQGVVVSGQN SSTPVPGGFE RIVENLKDEN VGIDLFTYQR 

       490        500        510        520        530 
MGALFFSPEH FHAFTVFVRN LSQFELSSDD QASEAEVEAE TASIGSGTGA PSLQTA 

« Hide

References

« Hide 'large scale' references
[1]"A novel putative beta-amylase gene and ATbeta-Amy from Arabidopsis thaliana are circadian regulated."
Chandler J.W., Apel K., Melzer S.
Plant Sci. 161:1019-1024(2001)
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
Strain: cv. Landsberg erecta.
[2]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed: 11130714] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Beta-AMYLASE4, a noncatalytic protein required for starch breakdown, acts upstream of three active beta-amylases in Arabidopsis chloroplasts."
Fulton D.C., Stettler M., Mettler T., Vaughan C.K., Li J., Francisco P., Gil M., Reinhold H., Eicke S., Messerli G., Dorken G., Halliday K., Smith A.M., Smith S.M., Zeeman S.C.
Plant Cell 20:1040-1058(2008) [PubMed: 18390594] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF402598 mRNA. Translation: AAK85300.1.
AC051627 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92597.1.
AY069879 mRNA. Translation: AAL47434.1.
AY142007 mRNA. Translation: AAM98271.1.
AY087036 mRNA. Translation: AAM64597.1.
IPIIPI00528458.
RefSeqNP_197368.1. NM_121872.2.
UniGeneAt.19910.

3D structure databases

HSSPHSSP built from PDB template 1FA2 based on UniProtKB P10537.
ProteinModelPortalQ8VYW2.
SMRQ8VYW2. Positions 83-524.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ8VYW2.

Protein family/group databases

CAZyGH14. Glycoside Hydrolase Family 14.

Proteomic databases

PRIDEQ8VYW2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G18670.1; AT5G18670.1; AT5G18670.
GeneID831985.
GenomeReviewsGene locus AT5G18670 in contig BA000015_GR.
KEGGath:AT5G18670.
NMPDRfig|3702.1.peg.24056.

Organism-specific databases

TAIRAt5g18670.

Phylogenomic databases

eggNOGNOG77898.
GeneTreeEPGT00070000028196.
InParanoidQ8VYW2.
OMAGNPLWGL.
PhylomeDBQ8VYW2.
ProtClustDBCLSN2916428.

Gene expression databases

GenevestigatorQ8VYW2.

Family and domain databases

InterProIPR001554. Glyco_hydro_14.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PfamPF01373. Glyco_hydro_14. 1 hit.
[Graphical view]
PRINTSPR00750. BETAAMYLASE.
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS00506. BETA_AMYLASE_1. False negative.
PS00679. BETA_AMYLASE_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBAM9_ARATH
AccessionPrimary (citable) accession number: Q8VYW2
Secondary accession number(s): Q946D4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: March 1, 2002
Last modified: December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families