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Protein

Purple acid phosphatase 4

Gene

PAP4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi53IronBy similarity1
Metal bindingi86IronBy similarity1
Metal bindingi86ZincBy similarity1
Metal bindingi89IronBy similarity1
Metal bindingi124ZincBy similarity1
Metal bindingi218ZincBy similarity1
Active sitei227Proton donorBy similarity1
Metal bindingi253ZincBy similarity1
Metal bindingi255IronBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT1G25230-MONOMER.
ReactomeiR-ATH-196843. Vitamin B2 (riboflavin) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 4 (EC:3.1.3.2)
Gene namesi
Name:PAP4
Ordered Locus Names:At1g25230
ORF Names:F4F7.38
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G25230.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000037280932 – 339Purple acid phosphatase 4Add BLAST308

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi61N-linked (GlcNAc...)Sequence analysis1
Glycosylationi284N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8VYU7.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Gene expression databases

GenevisibleiQ8VYU7. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G25230.1.

Structurei

3D structure databases

ProteinModelPortaliQ8VYU7.
SMRiQ8VYU7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni253 – 255Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2679. Eukaryota.
COG1409. LUCA.
HOGENOMiHOG000239892.
InParanoidiQ8VYU7.
OMAiGEEMDIN.
OrthoDBiEOG09360IST.
PhylomeDBiQ8VYU7.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VYU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSKFDIGSL SIVMTLLICF LLLSLAPKLE AELATVQHAP NPDGSISFLV
60 70 80 90 100
IGDWGRHGLY NQSQVALQMG RIGEEMDINF VVSTGDNIYD NGMKSIDDPA
110 120 130 140 150
FQLSFSNIYT SPSLQKPWYL VLGNHDYRGD VEAQLSPILR SMDSRWICMR
160 170 180 190 200
SFIVDAEIAE LFFVDTTPFV DAYFLSPQDQ TYDWSGVSPR KSYLQTILTE
210 220 230 240 250
LEMGLRESSA KWKIVVGHHA IKSASIHGNT KELESLLLPI LEANKVDLYM
260 270 280 290 300
NGHDHCLQHI STSQSPIQFL TSGGGSKAWR GYYNWTTPED MKFFYDGQGF
310 320 330
MSVKITRSEL SVVFYDVSGN SLHKWDTSKM LDSDFYFPL
Length:339
Mass (Da):38,241
Last modified:March 1, 2002 - v1
Checksum:iA801B23B57C92609
GO

Sequence cautioni

The sequence AAG28815 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY842021 mRNA. Translation: AAW29946.1.
AC079374 Genomic DNA. Translation: AAG28815.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30591.1.
AY069908 mRNA. Translation: AAL47459.1.
AY141997 mRNA. Translation: AAM98261.1.
PIRiH86381.
RefSeqiNP_173894.2. NM_102332.4.
UniGeneiAt.19616.

Genome annotation databases

EnsemblPlantsiAT1G25230.1; AT1G25230.1; AT1G25230.
GeneIDi839105.
GrameneiAT1G25230.1; AT1G25230.1; AT1G25230.
KEGGiath:AT1G25230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY842021 mRNA. Translation: AAW29946.1.
AC079374 Genomic DNA. Translation: AAG28815.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30591.1.
AY069908 mRNA. Translation: AAL47459.1.
AY141997 mRNA. Translation: AAM98261.1.
PIRiH86381.
RefSeqiNP_173894.2. NM_102332.4.
UniGeneiAt.19616.

3D structure databases

ProteinModelPortaliQ8VYU7.
SMRiQ8VYU7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G25230.1.

Proteomic databases

PaxDbiQ8VYU7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G25230.1; AT1G25230.1; AT1G25230.
GeneIDi839105.
GrameneiAT1G25230.1; AT1G25230.1; AT1G25230.
KEGGiath:AT1G25230.

Organism-specific databases

TAIRiAT1G25230.

Phylogenomic databases

eggNOGiKOG2679. Eukaryota.
COG1409. LUCA.
HOGENOMiHOG000239892.
InParanoidiQ8VYU7.
OMAiGEEMDIN.
OrthoDBiEOG09360IST.
PhylomeDBiQ8VYU7.

Enzyme and pathway databases

BioCyciARA:AT1G25230-MONOMER.
ReactomeiR-ATH-196843. Vitamin B2 (riboflavin) metabolism.

Miscellaneous databases

PROiQ8VYU7.

Gene expression databases

GenevisibleiQ8VYU7. AT.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPA4_ARATH
AccessioniPrimary (citable) accession number: Q8VYU7
Secondary accession number(s): Q9FRH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.