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Q8VYU7 (PPA4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Purple acid phosphatase 4

EC=3.1.3.2
Gene names
Name:PAP4
Ordered Locus Names:At1g25230
ORF Names:F4F7.38
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length339 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Secreted By similarity.

Tissue specificity

Expressed in roots, stems, leaves, flowers and siliques. Ref.1

Sequence similarities

Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Sequence caution

The sequence AAG28815.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandIron
Metal-binding
Zinc
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionacid phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 339308Purple acid phosphatase 4
PRO_0000372809

Regions

Region253 – 2553Substrate binding By similarity

Sites

Active site2271Proton donor By similarity
Metal binding531Iron By similarity
Metal binding861Iron By similarity
Metal binding861Zinc By similarity
Metal binding891Iron By similarity
Metal binding1241Zinc By similarity
Metal binding2181Zinc By similarity
Metal binding2531Zinc By similarity
Metal binding2551Iron By similarity

Amino acid modifications

Glycosylation611N-linked (GlcNAc...) Potential
Glycosylation2841N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q8VYU7 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: A801B23B57C92609

FASTA33938,241
        10         20         30         40         50         60 
MSSKFDIGSL SIVMTLLICF LLLSLAPKLE AELATVQHAP NPDGSISFLV IGDWGRHGLY 

        70         80         90        100        110        120 
NQSQVALQMG RIGEEMDINF VVSTGDNIYD NGMKSIDDPA FQLSFSNIYT SPSLQKPWYL 

       130        140        150        160        170        180 
VLGNHDYRGD VEAQLSPILR SMDSRWICMR SFIVDAEIAE LFFVDTTPFV DAYFLSPQDQ 

       190        200        210        220        230        240 
TYDWSGVSPR KSYLQTILTE LEMGLRESSA KWKIVVGHHA IKSASIHGNT KELESLLLPI 

       250        260        270        280        290        300 
LEANKVDLYM NGHDHCLQHI STSQSPIQFL TSGGGSKAWR GYYNWTTPED MKFFYDGQGF 

       310        320        330 
MSVKITRSEL SVVFYDVSGN SLHKWDTSKM LDSDFYFPL 

« Hide

References

« Hide 'large scale' references
[1]"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY842021 mRNA. Translation: AAW29946.1.
AC079374 Genomic DNA. Translation: AAG28815.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30591.1.
AY069908 mRNA. Translation: AAL47459.1.
AY141997 mRNA. Translation: AAM98261.1.
PIRH86381.
RefSeqNP_173894.2. NM_102332.3.
UniGeneAt.19616.

3D structure databases

ProteinModelPortalQ8VYU7.
SMRQ8VYU7. Positions 45-324.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT1G25230.1-P.

Proteomic databases

PaxDbQ8VYU7.
PRIDEQ8VYU7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G25230.1; AT1G25230.1; AT1G25230.
GeneID839105.
KEGGath:AT1G25230.

Organism-specific databases

TAIRAT1G25230.

Phylogenomic databases

eggNOGCOG1409.
HOGENOMHOG000239892.
InParanoidQ8VYU7.
OMAGEEMDIN.
PhylomeDBQ8VYU7.

Enzyme and pathway databases

BioCycARA:AT1G25230-MONOMER.

Gene expression databases

ArrayExpressQ8VYU7.
GenevestigatorQ8VYU7.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFPIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMSSF56300. SSF56300. 1 hit.
ProtoNetSearch...

Entry information

Entry namePPA4_ARATH
AccessionPrimary (citable) accession number: Q8VYU7
Secondary accession number(s): Q9FRH2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2002
Last modified: June 11, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names