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Q8VYU7

- PPA4_ARATH

UniProt

Q8VYU7 - PPA4_ARATH

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Protein
Purple acid phosphatase 4
Gene
PAP4, At1g25230, F4F7.38
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Binds 1 iron ion per subunit By similarity.
Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Iron By similarity
Metal bindingi86 – 861Iron By similarity
Metal bindingi86 – 861Zinc By similarity
Metal bindingi89 – 891Iron By similarity
Metal bindingi124 – 1241Zinc By similarity
Metal bindingi218 – 2181Zinc By similarity
Active sitei227 – 2271Proton donor By similarity
Metal bindingi253 – 2531Zinc By similarity
Metal bindingi255 – 2551Iron By similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Iron, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT1G25230-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Purple acid phosphatase 4 (EC:3.1.3.2)
    Gene namesi
    Name:PAP4
    Ordered Locus Names:At1g25230
    ORF Names:F4F7.38
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G25230.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131 Reviewed prediction
    Add
    BLAST
    Chaini32 – 339308Purple acid phosphatase 4
    PRO_0000372809Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi61 – 611N-linked (GlcNAc...) Reviewed prediction
    Glycosylationi284 – 2841N-linked (GlcNAc...) Reviewed prediction

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8VYU7.
    PRIDEiQ8VYU7.

    Expressioni

    Tissue specificityi

    Expressed in roots, stems, leaves, flowers and siliques.1 Publication

    Gene expression databases

    ArrayExpressiQ8VYU7.
    GenevestigatoriQ8VYU7.

    Interactioni

    Subunit structurei

    Homodimer By similarity.

    Protein-protein interaction databases

    STRINGi3702.AT1G25230.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VYU7.
    SMRiQ8VYU7. Positions 45-324.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni253 – 2553Substrate binding By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1409.
    HOGENOMiHOG000239892.
    InParanoidiQ8VYU7.
    OMAiGEEMDIN.
    PhylomeDBiQ8VYU7.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR024927. Acid_Pase_5.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000898. Acid_Ptase_5. 1 hit.
    SUPFAMiSSF56300. SSF56300. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8VYU7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSKFDIGSL SIVMTLLICF LLLSLAPKLE AELATVQHAP NPDGSISFLV    50
    IGDWGRHGLY NQSQVALQMG RIGEEMDINF VVSTGDNIYD NGMKSIDDPA 100
    FQLSFSNIYT SPSLQKPWYL VLGNHDYRGD VEAQLSPILR SMDSRWICMR 150
    SFIVDAEIAE LFFVDTTPFV DAYFLSPQDQ TYDWSGVSPR KSYLQTILTE 200
    LEMGLRESSA KWKIVVGHHA IKSASIHGNT KELESLLLPI LEANKVDLYM 250
    NGHDHCLQHI STSQSPIQFL TSGGGSKAWR GYYNWTTPED MKFFYDGQGF 300
    MSVKITRSEL SVVFYDVSGN SLHKWDTSKM LDSDFYFPL 339
    Length:339
    Mass (Da):38,241
    Last modified:March 1, 2002 - v1
    Checksum:iA801B23B57C92609
    GO

    Sequence cautioni

    The sequence AAG28815.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY842021 mRNA. Translation: AAW29946.1.
    AC079374 Genomic DNA. Translation: AAG28815.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE30591.1.
    AY069908 mRNA. Translation: AAL47459.1.
    AY141997 mRNA. Translation: AAM98261.1.
    PIRiH86381.
    RefSeqiNP_173894.2. NM_102332.3.
    UniGeneiAt.19616.

    Genome annotation databases

    EnsemblPlantsiAT1G25230.1; AT1G25230.1; AT1G25230.
    GeneIDi839105.
    KEGGiath:AT1G25230.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY842021 mRNA. Translation: AAW29946.1 .
    AC079374 Genomic DNA. Translation: AAG28815.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE30591.1 .
    AY069908 mRNA. Translation: AAL47459.1 .
    AY141997 mRNA. Translation: AAM98261.1 .
    PIRi H86381.
    RefSeqi NP_173894.2. NM_102332.3.
    UniGenei At.19616.

    3D structure databases

    ProteinModelPortali Q8VYU7.
    SMRi Q8VYU7. Positions 45-324.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G25230.1-P.

    Proteomic databases

    PaxDbi Q8VYU7.
    PRIDEi Q8VYU7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G25230.1 ; AT1G25230.1 ; AT1G25230 .
    GeneIDi 839105.
    KEGGi ath:AT1G25230.

    Organism-specific databases

    TAIRi AT1G25230.

    Phylogenomic databases

    eggNOGi COG1409.
    HOGENOMi HOG000239892.
    InParanoidi Q8VYU7.
    OMAi GEEMDIN.
    PhylomeDBi Q8VYU7.

    Enzyme and pathway databases

    BioCyci ARA:AT1G25230-MONOMER.

    Gene expression databases

    ArrayExpressi Q8VYU7.
    Genevestigatori Q8VYU7.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR024927. Acid_Pase_5.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000898. Acid_Ptase_5. 1 hit.
    SUPFAMi SSF56300. SSF56300. 1 hit.
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
      Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
      Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
      Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
      J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPPA4_ARATH
    AccessioniPrimary (citable) accession number: Q8VYU7
    Secondary accession number(s): Q9FRH2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: March 1, 2002
    Last modified: June 11, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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