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Q8VYN6

- PFKA5_ARATH

UniProt

Q8VYN6 - PFKA5_ARATH

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Protein

ATP-dependent 6-phosphofructokinase 5, chloroplastic

Gene

PFK5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.1 PublicationUniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei189 – 1891ATP; via amide nitrogenUniRule annotation
Metal bindingi279 – 2791Magnesium; catalyticUniRule annotation
Sitei280 – 2801Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei309 – 3091Proton acceptorUniRule annotation
Binding sitei408 – 4081SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi253 – 2542ATPUniRule annotation
Nucleotide bindingi278 – 2814ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: TAIR
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. fructose 6-phosphate metabolic process Source: InterPro
  3. glycolytic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G22480-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 5, chloroplasticUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 5UniRule annotation
Short name:
Phosphofructokinase 5UniRule annotation
Alternative name(s):
Phosphohexokinase 5UniRule annotation
Gene namesi
Name:PFK5UniRule annotation
Ordered Locus Names:At2g22480
ORF Names:F14M13.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G22480.

Subcellular locationi

Plastidchloroplast 1 Publication

GO - Cellular componenti

  1. chloroplast Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5252ChloroplastSequence AnalysisAdd
BLAST
Chaini53 – 537485ATP-dependent 6-phosphofructokinase 5, chloroplasticPRO_0000330772Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei147 – 1471PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8VYN6.
PRIDEiQ8VYN6.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems and flowers.1 Publication

Gene expression databases

GenevestigatoriQ8VYN6.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

BioGridi2134. 1 interaction.
STRINGi3702.AT2G22480.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8VYN6.
SMRiQ8VYN6. Positions 167-526.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni307 – 3093Substrate bindingUniRule annotation
Regioni352 – 3543Substrate bindingUniRule annotation
Regioni460 – 4634Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000016186.
InParanoidiQ8VYN6.
KOiK00850.
OMAiNEICKAM.
PhylomeDBiQ8VYN6.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFruKinase_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VYN6-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MDALSQAISS GISVPYKNNS SSLVPSHGLT SLILRKSRSP VNPSSRSRVS
60 70 80 90 100
VRASEIQHSK TSASSIDLSD PDWKLKYEKD FEQRFSIPHI TDVLPDAEAI
110 120 130 140 150
RSTFCLKMRS PTEDFVGGYP SDEEWHGYIN NNDRVLLKVI SYSSPTSAGA
160 170 180 190 200
ECLDHDCSWV EQWIHRAGPR EKIYFRPEEV KAAIITCGGL CPGLNDVIRH
210 220 230 240 250
IVITLEIYGV KNIVGIPFGY RGFSDKDLTE MPLSRKVVQN IHLSGGSLLG
260 270 280 290 300
VSRGGPSVSE IVDSMEERGI NMLFVLGGNG THAGANAIHN ECRKRKIKVA
310 320 330 340 350
VVGVPKTIDN DILHMDKTFG FDTAVEEAQR AINSAYIEAH SAYHGIGVVK
360 370 380 390 400
LMGRNSGFIA MQASLASGQV DICLIPEVPF NLHGPNGVLK HLKYLIETKG
410 420 430 440 450
SAVICVAEGA GQNFLEKTNA KDASGNAVLG DFGVYIQQET KKYFKEISTP
460 470 480 490 500
IDVKYIDPTY MIRAVRANAS DGILCTVLGQ NAVHGAFAGY SGITVGIINT
510 520 530
HYAYLPITEV IAYPKSVDPN SRMWHRCLTS TGQPDFI
Length:537
Mass (Da):58,615
Last modified:March 1, 2002 - v1
Checksum:i5A9DD3004D1ACC6A
GO

Sequence cautioni

The sequence AAD22353.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC006592 Genomic DNA. Translation: AAD22353.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC07312.1.
AY070402 mRNA. Translation: AAL49898.1.
AY096578 mRNA. Translation: AAM20228.1.
PIRiB84613.
RefSeqiNP_850025.1. NM_179694.3.
UniGeneiAt.28370.

Genome annotation databases

EnsemblPlantsiAT2G22480.1; AT2G22480.1; AT2G22480.
GeneIDi816781.
KEGGiath:AT2G22480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC006592 Genomic DNA. Translation: AAD22353.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC07312.1 .
AY070402 mRNA. Translation: AAL49898.1 .
AY096578 mRNA. Translation: AAM20228.1 .
PIRi B84613.
RefSeqi NP_850025.1. NM_179694.3.
UniGenei At.28370.

3D structure databases

ProteinModelPortali Q8VYN6.
SMRi Q8VYN6. Positions 167-526.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 2134. 1 interaction.
STRINGi 3702.AT2G22480.1-P.

Proteomic databases

PaxDbi Q8VYN6.
PRIDEi Q8VYN6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G22480.1 ; AT2G22480.1 ; AT2G22480 .
GeneIDi 816781.
KEGGi ath:AT2G22480.

Organism-specific databases

TAIRi AT2G22480.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000016186.
InParanoidi Q8VYN6.
KOi K00850.
OMAi NEICKAM.
PhylomeDBi Q8VYN6.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci ARA:AT2G22480-MONOMER.

Gene expression databases

Genevestigatori Q8VYN6.

Family and domain databases

HAMAPi MF_01981. Phosphofructokinase_II_X.
InterProi IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFruKinase_TP0108.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana."
    Mustroph A., Sonnewald U., Biemelt S.
    FEBS Lett. 581:2401-2410(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPFKA5_ARATH
AccessioniPrimary (citable) accession number: Q8VYN6
Secondary accession number(s): Q9SJY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2002
Last modified: October 29, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3