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Q8VYN6

- PFKA5_ARATH

UniProt

Q8VYN6 - PFKA5_ARATH

Protein

ATP-dependent 6-phosphofructokinase 5, chloroplastic

Gene

PFK5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.1 PublicationUniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by AMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei189 – 1891ATP; via amide nitrogenUniRule annotation
    Metal bindingi279 – 2791Magnesium; catalyticUniRule annotation
    Sitei280 – 2801Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
    Active sitei309 – 3091Proton acceptorUniRule annotation
    Binding sitei408 – 4081SubstrateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi253 – 2542ATPUniRule annotation
    Nucleotide bindingi278 – 2814ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: TAIR
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate phosphorylation Source: GOC
    2. fructose 6-phosphate metabolic process Source: InterPro
    3. glycolytic process Source: TAIR

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G22480-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase 5, chloroplasticUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFK 5UniRule annotation
    Short name:
    Phosphofructokinase 5UniRule annotation
    Alternative name(s):
    Phosphohexokinase 5UniRule annotation
    Gene namesi
    Name:PFK5UniRule annotation
    Ordered Locus Names:At2g22480
    ORF Names:F14M13.12
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G22480.

    Subcellular locationi

    Plastidchloroplast 1 Publication

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro
    2. chloroplast Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5252ChloroplastSequence AnalysisAdd
    BLAST
    Chaini53 – 537485ATP-dependent 6-phosphofructokinase 5, chloroplasticPRO_0000330772Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei147 – 1471PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8VYN6.
    PRIDEiQ8VYN6.

    Expressioni

    Tissue specificityi

    Expressed in roots, leaves, stems and flowers.1 Publication

    Gene expression databases

    GenevestigatoriQ8VYN6.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    BioGridi2134. 1 interaction.
    STRINGi3702.AT2G22480.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VYN6.
    SMRiQ8VYN6. Positions 167-526.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni307 – 3093Substrate bindingUniRule annotation
    Regioni352 – 3543Substrate bindingUniRule annotation
    Regioni460 – 4634Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000016186.
    InParanoidiQ8VYN6.
    KOiK00850.
    OMAiNEICKAM.
    PhylomeDBiQ8VYN6.

    Family and domain databases

    HAMAPiMF_01981. Phosphofructokinase_II_X.
    InterProiIPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    IPR012004. PyroP-dep_PFruKinase_TP0108.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8VYN6-1 [UniParc]FASTAAdd to Basket

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    MDALSQAISS GISVPYKNNS SSLVPSHGLT SLILRKSRSP VNPSSRSRVS    50
    VRASEIQHSK TSASSIDLSD PDWKLKYEKD FEQRFSIPHI TDVLPDAEAI 100
    RSTFCLKMRS PTEDFVGGYP SDEEWHGYIN NNDRVLLKVI SYSSPTSAGA 150
    ECLDHDCSWV EQWIHRAGPR EKIYFRPEEV KAAIITCGGL CPGLNDVIRH 200
    IVITLEIYGV KNIVGIPFGY RGFSDKDLTE MPLSRKVVQN IHLSGGSLLG 250
    VSRGGPSVSE IVDSMEERGI NMLFVLGGNG THAGANAIHN ECRKRKIKVA 300
    VVGVPKTIDN DILHMDKTFG FDTAVEEAQR AINSAYIEAH SAYHGIGVVK 350
    LMGRNSGFIA MQASLASGQV DICLIPEVPF NLHGPNGVLK HLKYLIETKG 400
    SAVICVAEGA GQNFLEKTNA KDASGNAVLG DFGVYIQQET KKYFKEISTP 450
    IDVKYIDPTY MIRAVRANAS DGILCTVLGQ NAVHGAFAGY SGITVGIINT 500
    HYAYLPITEV IAYPKSVDPN SRMWHRCLTS TGQPDFI 537
    Length:537
    Mass (Da):58,615
    Last modified:March 1, 2002 - v1
    Checksum:i5A9DD3004D1ACC6A
    GO

    Sequence cautioni

    The sequence AAD22353.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006592 Genomic DNA. Translation: AAD22353.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC07312.1.
    AY070402 mRNA. Translation: AAL49898.1.
    AY096578 mRNA. Translation: AAM20228.1.
    PIRiB84613.
    RefSeqiNP_850025.1. NM_179694.3.
    UniGeneiAt.28370.

    Genome annotation databases

    EnsemblPlantsiAT2G22480.1; AT2G22480.1; AT2G22480.
    GeneIDi816781.
    KEGGiath:AT2G22480.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006592 Genomic DNA. Translation: AAD22353.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC07312.1 .
    AY070402 mRNA. Translation: AAL49898.1 .
    AY096578 mRNA. Translation: AAM20228.1 .
    PIRi B84613.
    RefSeqi NP_850025.1. NM_179694.3.
    UniGenei At.28370.

    3D structure databases

    ProteinModelPortali Q8VYN6.
    SMRi Q8VYN6. Positions 167-526.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 2134. 1 interaction.
    STRINGi 3702.AT2G22480.1-P.

    Proteomic databases

    PaxDbi Q8VYN6.
    PRIDEi Q8VYN6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G22480.1 ; AT2G22480.1 ; AT2G22480 .
    GeneIDi 816781.
    KEGGi ath:AT2G22480.

    Organism-specific databases

    TAIRi AT2G22480.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000016186.
    InParanoidi Q8VYN6.
    KOi K00850.
    OMAi NEICKAM.
    PhylomeDBi Q8VYN6.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci ARA:AT2G22480-MONOMER.

    Gene expression databases

    Genevestigatori Q8VYN6.

    Family and domain databases

    HAMAPi MF_01981. Phosphofructokinase_II_X.
    InterProi IPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    IPR012004. PyroP-dep_PFruKinase_TP0108.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000534. PPi_PFK_TP0108. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana."
      Mustroph A., Sonnewald U., Biemelt S.
      FEBS Lett. 581:2401-2410(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPFKA5_ARATH
    AccessioniPrimary (citable) accession number: Q8VYN6
    Secondary accession number(s): Q9SJY6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3