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Protein

ATP-dependent 6-phosphofructokinase 5, chloroplastic

Gene

PFK5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (MJB21.12), Glucose-6-phosphate isomerase (AXX17_At5g40630), Glucose-6-phosphate isomerase (PGI1 (At4g24620)), Glucose-6-phosphate isomerase, cytosolic (PGIC), Glucose-6-phosphate isomerase (AXX17_At4g28500), Glucose-6-phosphate isomerase 1, chloroplastic (PGI1), Glucose-6-phosphate isomerase (PGIC)
  3. ATP-dependent 6-phosphofructokinase 1 (PFK1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 3 (PFK3), ATP-dependent 6-phosphofructokinase 4, chloroplastic (PFK4), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase 2 (PFK2), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 (PFP-BETA2), ATP-dependent 6-phosphofructokinase 5, chloroplastic (PFK5), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 (PFP-ALPHA1), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase 6 (PFK6), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 (PFP-ALPHA2), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 (PFP-BETA1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 7 (PFK7), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase 5, cytosolic (FBA5), Fructose-bisphosphate aldolase (FBA6), Fructose-bisphosphate aldolase (FBA4), Fructose-bisphosphate aldolase (AXX17_At4g44320), Fructose-bisphosphate aldolase (AXX17_At4g30610), Fructose-bisphosphate aldolase (FBA2), Fructose-bisphosphate aldolase 1, chloroplastic (FBA1), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 7, cytosolic (FBA7), Fructose-bisphosphate aldolase 3, chloroplastic (FBA3), Fructose-bisphosphate aldolase 6, cytosolic (FBA6), Fructose-bisphosphate aldolase (FBA7), Fructose-bisphosphate aldolase (AXX17_At2g00100), Fructose-bisphosphate aldolase 2, chloroplastic (FBA2), Fructose-bisphosphate aldolase (At2g21330), Fructose-bisphosphate aldolase (AXX17_At2g33200), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (AXX17_At2g16800), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 8, cytosolic (FBA8), Fructose-bisphosphate aldolase (FBA7), Fructose-bisphosphate aldolase (AXX17_At4g30600), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase 4, cytosolic (FBA4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189ATP; via amide nitrogenUniRule annotation1
Metal bindingi279Magnesium; catalyticUniRule annotation1
Sitei280Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei309Proton acceptorUniRule annotation1
Binding sitei408SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi253 – 254ATPUniRule annotation2
Nucleotide bindingi278 – 281ATPUniRule annotation4

GO - Molecular functioni

  • 6-phosphofructokinase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • fructose 6-phosphate metabolic process Source: InterPro
  • glycolytic process Source: TAIR

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G22480-MONOMER
BRENDAi2.7.1.11 399
ReactomeiR-ATH-6798695 Neutrophil degranulation
R-ATH-70171 Glycolysis
UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 5, chloroplasticUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 5UniRule annotation
Short name:
Phosphofructokinase 5UniRule annotation
Alternative name(s):
Phosphohexokinase 5UniRule annotation
Gene namesi
Name:PFK5UniRule annotation
Ordered Locus Names:At2g22480
ORF Names:F14M13.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi

Organism-specific databases

AraportiAT2G22480
TAIRilocus:2041208 AT2G22480

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 52ChloroplastSequence analysisAdd BLAST52
ChainiPRO_000033077253 – 537ATP-dependent 6-phosphofructokinase 5, chloroplasticAdd BLAST485

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei147PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8VYN6
PRIDEiQ8VYN6

PTM databases

iPTMnetiQ8VYN6

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems and flowers.1 Publication

Gene expression databases

ExpressionAtlasiQ8VYN6 baseline and differential
GenevisibleiQ8VYN6 AT

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

BioGridi2134, 1 interactor
STRINGi3702.AT2G22480.1

Structurei

3D structure databases

ProteinModelPortaliQ8VYN6
SMRiQ8VYN6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni307 – 309Substrate bindingUniRule annotation3
Regioni352 – 354Substrate bindingUniRule annotation3
Regioni460 – 463Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2440 Eukaryota
COG0205 LUCA
HOGENOMiHOG000016186
InParanoidiQ8VYN6
KOiK00850
OMAiHITDIYD
OrthoDBiEOG093606YN
PhylomeDBiQ8VYN6

Family and domain databases

HAMAPiMF_01981 Phosphofructokinase_II_X, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
IPR012004 PyroP-dep_PFK_TP0108
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000534 PPi_PFK_TP0108, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VYN6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDALSQAISS GISVPYKNNS SSLVPSHGLT SLILRKSRSP VNPSSRSRVS
60 70 80 90 100
VRASEIQHSK TSASSIDLSD PDWKLKYEKD FEQRFSIPHI TDVLPDAEAI
110 120 130 140 150
RSTFCLKMRS PTEDFVGGYP SDEEWHGYIN NNDRVLLKVI SYSSPTSAGA
160 170 180 190 200
ECLDHDCSWV EQWIHRAGPR EKIYFRPEEV KAAIITCGGL CPGLNDVIRH
210 220 230 240 250
IVITLEIYGV KNIVGIPFGY RGFSDKDLTE MPLSRKVVQN IHLSGGSLLG
260 270 280 290 300
VSRGGPSVSE IVDSMEERGI NMLFVLGGNG THAGANAIHN ECRKRKIKVA
310 320 330 340 350
VVGVPKTIDN DILHMDKTFG FDTAVEEAQR AINSAYIEAH SAYHGIGVVK
360 370 380 390 400
LMGRNSGFIA MQASLASGQV DICLIPEVPF NLHGPNGVLK HLKYLIETKG
410 420 430 440 450
SAVICVAEGA GQNFLEKTNA KDASGNAVLG DFGVYIQQET KKYFKEISTP
460 470 480 490 500
IDVKYIDPTY MIRAVRANAS DGILCTVLGQ NAVHGAFAGY SGITVGIINT
510 520 530
HYAYLPITEV IAYPKSVDPN SRMWHRCLTS TGQPDFI
Length:537
Mass (Da):58,615
Last modified:March 1, 2002 - v1
Checksum:i5A9DD3004D1ACC6A
GO

Sequence cautioni

The sequence AAD22353 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006592 Genomic DNA Translation: AAD22353.1 Sequence problems.
CP002685 Genomic DNA Translation: AEC07312.1
AY070402 mRNA Translation: AAL49898.1
AY096578 mRNA Translation: AAM20228.1
PIRiB84613
RefSeqiNP_850025.1, NM_179694.4
UniGeneiAt.28370

Genome annotation databases

EnsemblPlantsiAT2G22480.1; AT2G22480.1; AT2G22480
GeneIDi816781
GrameneiAT2G22480.1; AT2G22480.1; AT2G22480
KEGGiath:AT2G22480

Similar proteinsi

Entry informationi

Entry nameiPFKA5_ARATH
AccessioniPrimary (citable) accession number: Q8VYN6
Secondary accession number(s): Q9SJY6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2002
Last modified: April 25, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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