Reviewed, UniProtKB/Swiss-Prot Q8VYN6 (K6PF5_ARATH)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase 5, chloroplastic
      Short name=Phosphofructokinase 5
    EC=2.7.1.11
Alternative name(s):
    Phosphohexokinase 5

Gene names

Name:	PFK5
Ordered Locus Names:	At2g22480
ORF Names:	F14M13.12

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. Ref.3
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subcellular location	Plastid › chloroplast Ref.3.
Tissue specificity	Expressed in roots, leaves, stems and flowers. Ref.3
Sequence similarities	Belongs to the phosphofructokinase family.
Sequence caution	The sequence AAD22353.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro chloroplast Ref.3 Inferred from direct assay. Source: TAIR
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 52	52	Chloroplast Potential
	Chain	53 – 537	485	6-phosphofructokinase 5, chloroplastic		PRO_0000330772
Regions
	Nucleotide binding	199 – 203	5	ATP By similarity
	Nucleotide binding	336 – 340	5	ATP By similarity
	Nucleotide binding	354 – 371	18	ATP By similarity
Sites
	Active site	309	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8VYN6-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 5A9DD3004D1ACC6A	FASTA	537	58,615
	10 20 30 40 50 60 MDALSQAISS GISVPYKNNS SSLVPSHGLT SLILRKSRSP VNPSSRSRVS VRASEIQHSK 70 80 90 100 110 120 TSASSIDLSD PDWKLKYEKD FEQRFSIPHI TDVLPDAEAI RSTFCLKMRS PTEDFVGGYP 130 140 150 160 170 180 SDEEWHGYIN NNDRVLLKVI SYSSPTSAGA ECLDHDCSWV EQWIHRAGPR EKIYFRPEEV 190 200 210 220 230 240 KAAIITCGGL CPGLNDVIRH IVITLEIYGV KNIVGIPFGY RGFSDKDLTE MPLSRKVVQN 250 260 270 280 290 300 IHLSGGSLLG VSRGGPSVSE IVDSMEERGI NMLFVLGGNG THAGANAIHN ECRKRKIKVA 310 320 330 340 350 360 VVGVPKTIDN DILHMDKTFG FDTAVEEAQR AINSAYIEAH SAYHGIGVVK LMGRNSGFIA 370 380 390 400 410 420 MQASLASGQV DICLIPEVPF NLHGPNGVLK HLKYLIETKG SAVICVAEGA GQNFLEKTNA 430 440 450 460 470 480 KDASGNAVLG DFGVYIQQET KKYFKEISTP IDVKYIDPTY MIRAVRANAS DGILCTVLGQ 490 500 510 520 530 NAVHGAFAGY SGITVGIINT HYAYLPITEV IAYPKSVDPN SRMWHRCLTS TGQPDFI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana." Mustroph A., Sonnewald U., Biemelt S. FEBS Lett. 581:2401-2410(2007) [PubMed: 17485088] [Abstract] Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006592 Genomic DNA. Translation: AAD22353.1. Sequence problems. AY070402 mRNA. Translation: AAL49898.1. AY096578 mRNA. Translation: AAM20228.1.
IPI	IPI00542260.
PIR	B84613.
RefSeq	NP_850025.1.
UniGene	At.28370 Rra.24173
3D structure databases
HSSP	HSSP built from PDB template 3PFK based on UniProtKB P00512.
SMR	Q8VYN6. Positions 125-531.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8VYN6.
Proteomic databases
PRIDE	Q8VYN6.
Genome annotation databases
GeneID	816781.
GenomeReviews	Gene locus AT2G22480 in contig CT485783_GR.
KEGG	ath:AT2G22480.
NMPDR	fig|3702.1.peg.9268.
Organism-specific databases
TAIR	At2g22480.
Phylogenomic databases
eggNOG	KOG2440.
HOGENOM	HBG316947.
InParanoid	Q8VYN6.
OMA	AGAECID.
PhylomeDB	Q8VYN6.
Gene expression databases
Genevestigator	Q8VYN6.
Family and domain databases
InterPro	IPR000023. Phosphofructokinase. IPR012004. PyroP-dep_PFruKinase_TP0108. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
ProtoNet	Search...

Entry information

Entry name

K6PF5_ARATH

Accession

Primary (citable) accession number: Q8VYN6
Secondary accession number(s): Q9SJY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)