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Protein

Inorganic phosphate transporter 1-1

Gene

PHT1-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity transporter for external inorganic phosphate. Acts as a H+:phosphate symporter in both low- and high-Pi conditions. Confers sensitivity to arsenate.3 Publications

Enzyme regulationi

Inhibited by protonophores (e.g. 2,4-dinitrophenol and carbonylcyanide m-chlorophenylhydrazone), the plasma membrane H+-ATPase inhibitor diethylstilbestorol, and the phosphate analog arsenate.1 Publication

Kineticsi

  1. KM=3 µM for inorganic phosphate1 Publication

    GO - Molecular functioni

    • arsenate ion transmembrane transporter activity Source: TAIR
    • inorganic phosphate transmembrane transporter activity Source: TAIR
    • phosphate ion transmembrane transporter activity Source: TAIR
    • symporter activity Source: UniProtKB-KW

    GO - Biological processi

    • arsenate ion transmembrane transport Source: TAIR
    • cellular response to phosphate starvation Source: TAIR
    • phosphate ion transmembrane transport Source: GOC
    • phosphate ion transport Source: TAIR
    Complete GO annotation...

    Keywords - Biological processi

    Phosphate transport, Symport, Transport

    Enzyme and pathway databases

    BioCyciARA:GQT-2848-MONOMER.

    Protein family/group databases

    TCDBi2.A.1.9.9. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inorganic phosphate transporter 1-1
    Short name:
    AtPht1;1
    Alternative name(s):
    H(+)/Pi cotransporter
    Gene namesi
    Name:PHT1-1
    Synonyms:APT2, PHT1, PT1
    Ordered Locus Names:At5g43350
    ORF Names:MWF20.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    TAIRiAT5G43350.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2424CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei25 – 4521HelicalSequence analysisAdd
    BLAST
    Topological domaini46 – 7025ExtracellularSequence analysisAdd
    BLAST
    Transmembranei71 – 9121HelicalSequence analysisAdd
    BLAST
    Topological domaini92 – 998CytoplasmicSequence analysis
    Transmembranei100 – 12021HelicalSequence analysisAdd
    BLAST
    Topological domaini121 – 13111ExtracellularSequence analysisAdd
    BLAST
    Transmembranei132 – 15221HelicalSequence analysisAdd
    BLAST
    Topological domaini153 – 1619CytoplasmicSequence analysis
    Transmembranei162 – 18221HelicalSequence analysisAdd
    BLAST
    Topological domaini183 – 21129ExtracellularSequence analysisAdd
    BLAST
    Transmembranei212 – 23221HelicalSequence analysisAdd
    BLAST
    Topological domaini233 – 29260CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei293 – 31321HelicalSequence analysisAdd
    BLAST
    Topological domaini314 – 34835ExtracellularSequence analysisAdd
    BLAST
    Transmembranei349 – 36921HelicalSequence analysisAdd
    BLAST
    Topological domaini370 – 3712CytoplasmicSequence analysis
    Transmembranei372 – 39221HelicalSequence analysisAdd
    BLAST
    Topological domaini393 – 40210ExtracellularSequence analysis
    Transmembranei403 – 42321HelicalSequence analysisAdd
    BLAST
    Topological domaini424 – 44118CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei442 – 46221HelicalSequence analysisAdd
    BLAST
    Topological domaini463 – 48422ExtracellularSequence analysisAdd
    BLAST
    Transmembranei485 – 50521HelicalSequence analysisAdd
    BLAST
    Topological domaini506 – 52419CytoplasmicSequence analysisAdd
    BLAST

    GO - Cellular componenti

    • integral component of plasma membrane Source: GO_Central
    • plasma membrane Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 524524Inorganic phosphate transporter 1-1PRO_0000050468Add
    BLAST

    Proteomic databases

    PaxDbiQ8VYM2.
    PRIDEiQ8VYM2.

    PTM databases

    iPTMnetiQ8VYM2.

    Expressioni

    Tissue specificityi

    Mostly expressed in roots, especially in trichoblasts and in emerging secondary roots and root hairs, but not in root tips. Also present in hydathodes, axillary buds and peripheral endosperm of germinating seeds.6 Publications

    Inductioni

    In roots by phosphate starvation. Repressed by auxin, cytokinins, and the Pi analog phosphite (Phi).7 Publications

    Gene expression databases

    GenevisibleiQ8VYM2. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi19603. 5 interactions.
    MINTiMINT-8071073.
    STRINGi3702.AT5G43350.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VYM2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG0252. Eukaryota.
    ENOG410ZVN7. LUCA.
    HOGENOMiHOG000171120.
    InParanoidiQ8VYM2.
    KOiK08176.
    OMAiHIMEIFA.
    PhylomeDBiQ8VYM2.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR005828. MFS_sugar_transport_like.
    IPR004738. Phos_permease.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00887. 2A0109. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8VYM2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAEQQLGVLK ALDVAKTQLY HFTAIVIAGM GFFTDAYDLF CVSLVTKLLG
    60 70 80 90 100
    RIYYFNPESA KPGSLPPHVA AAVNGVALCG TLSGQLFFGW LGDKLGRKKV
    110 120 130 140 150
    YGLTLVMMIL CSVASGLSFG HEAKGVMTTL CFFRFWLGFG IGGDYPLSAT
    160 170 180 190 200
    IMSEYANKKT RGAFIAAVFA MQGVGILAGG FVALAVSSIF DKKFPAPTYA
    210 220 230 240 250
    VNRALSTPPQ VDYIWRIIVM FGALPAALTY YWRMKMPETA RYTALVAKNI
    260 270 280 290 300
    KQATADMSKV LQTDIELEER VEDDVKDPKQ NYGLFSKEFL RRHGLHLLGT
    310 320 330 340 350
    TSTWFLLDIA FYSQNLFQKD IFSAIGWIPK AATMNATHEV FRIARAQTLI
    360 370 380 390 400
    ALCSTVPGYW FTVAFIDTIG RFKIQLNGFF MMTVFMFAIA FPYNHWIKPE
    410 420 430 440 450
    NRIGFVVMYS LTFFFANFGP NATTFIVPAE IFPARLRSTC HGISAAAGKA
    460 470 480 490 500
    GAIVGAFGFL YAAQSQDKAK VDAGYPPGIG VKNSLIMLGV LNFIGMLFTF
    510 520
    LVPEPKGKSL EELSGEAEVS HDEK
    Length:524
    Mass (Da):57,616
    Last modified:January 10, 2006 - v2
    Checksum:i2D12D50987127BD6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti185 – 1851A → T in AAL49927 (PubMed:14593172).Curated
    Sequence conflicti469 – 4691A → P in CAA68946 (PubMed:9025304).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U62330 mRNA. Translation: AAB17265.1.
    Y07682 Genomic DNA. Translation: CAA68946.1.
    D86591 mRNA. Translation: BAA21503.1.
    D86608 Genomic DNA. Translation: BAA21504.1.
    AB025638 Genomic DNA. Translation: BAA97414.1.
    CP002688 Genomic DNA. Translation: AED94948.1.
    AY070432 mRNA. Translation: AAL49927.1.
    RefSeqiNP_199149.1. NM_123701.3.
    UniGeneiAt.49125.
    At.75586.

    Genome annotation databases

    EnsemblPlantsiAT5G43350.1; AT5G43350.1; AT5G43350.
    GeneIDi834353.
    GrameneiAT5G43350.1; AT5G43350.1; AT5G43350.
    KEGGiath:AT5G43350.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U62330 mRNA. Translation: AAB17265.1.
    Y07682 Genomic DNA. Translation: CAA68946.1.
    D86591 mRNA. Translation: BAA21503.1.
    D86608 Genomic DNA. Translation: BAA21504.1.
    AB025638 Genomic DNA. Translation: BAA97414.1.
    CP002688 Genomic DNA. Translation: AED94948.1.
    AY070432 mRNA. Translation: AAL49927.1.
    RefSeqiNP_199149.1. NM_123701.3.
    UniGeneiAt.49125.
    At.75586.

    3D structure databases

    ProteinModelPortaliQ8VYM2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi19603. 5 interactions.
    MINTiMINT-8071073.
    STRINGi3702.AT5G43350.1.

    Protein family/group databases

    TCDBi2.A.1.9.9. the major facilitator superfamily (mfs).

    PTM databases

    iPTMnetiQ8VYM2.

    Proteomic databases

    PaxDbiQ8VYM2.
    PRIDEiQ8VYM2.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G43350.1; AT5G43350.1; AT5G43350.
    GeneIDi834353.
    GrameneiAT5G43350.1; AT5G43350.1; AT5G43350.
    KEGGiath:AT5G43350.

    Organism-specific databases

    TAIRiAT5G43350.

    Phylogenomic databases

    eggNOGiKOG0252. Eukaryota.
    ENOG410ZVN7. LUCA.
    HOGENOMiHOG000171120.
    InParanoidiQ8VYM2.
    KOiK08176.
    OMAiHIMEIFA.
    PhylomeDBiQ8VYM2.

    Enzyme and pathway databases

    BioCyciARA:GQT-2848-MONOMER.

    Miscellaneous databases

    PROiQ8VYM2.

    Gene expression databases

    GenevisibleiQ8VYM2. AT.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR005828. MFS_sugar_transport_like.
    IPR004738. Phos_permease.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00887. 2A0109. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Phosphate transporters from the higher plant Arabidopsis thaliana."
      Muchhal U.S., Pardo J.M., Raghothama K.G.
      Proc. Natl. Acad. Sci. U.S.A. 93:10519-10523(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
      Strain: cv. Columbia.
      Tissue: Root.
    2. "The cloning of two Arabidopsis genes belonging to a phosphate transporter family."
      Smith F.W., Ealing P.M., Dong B., Delhaize E.
      Plant J. 11:83-92(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION.
      Strain: cv. Columbia.
      Tissue: Root.
    3. "Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions."
      Mitsukawa N., Okumura S., Shirano Y., Sato S., Kato T., Harashima S., Shibata D.
      Proc. Natl. Acad. Sci. U.S.A. 94:7098-7102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.
      Strain: cv. Columbia.
    4. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
      DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    7. "Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis."
      Mudge S.R., Rae A.L., Diatloff E., Smith F.W.
      Plant J. 31:341-353(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
    8. "Phosphite, an analog of phosphate, suppresses the coordinated expression of genes under phosphate starvation."
      Varadarajan D.K., Karthikeyan A.S., Matilda P.D., Raghothama K.G.
      Plant Physiol. 129:1232-1240(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    9. Cited for: TISSUE SPECIFICITY, INDUCTION.
    10. "Phosphate transport in Arabidopsis: Pht1;1 and Pht1;4 play a major role in phosphate acquisition from both low- and high-phosphate environments."
      Shin H., Shin H.-S., Dewbre G.R., Harrison M.J.
      Plant J. 39:629-642(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    11. "Promoter of Arabidopsis thaliana phosphate transporter gene drives root-specific expression of transgene in rice."
      Koyama T., Ono T., Shimizu M., Jinbo T., Mizuno R., Tomita K., Mitsukawa N., Kawazu T., Kimura T., Ohmiya K., Sakka K.
      J. Biosci. Bioeng. 99:38-42(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.
    12. "PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1 is a plant-specific SEC12-related protein that enables the endoplasmic reticulum exit of a high-affinity phosphate transporter in Arabidopsis."
      Gonzalez E., Solano R., Rubio V., Leyva A., Paz-Ares J.
      Plant Cell 17:3500-3512(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPHT11_ARATH
    AccessioniPrimary (citable) accession number: Q8VYM2
    Secondary accession number(s): Q96264, Q96302
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: January 10, 2006
    Last modified: February 17, 2016
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Although related to the sugar transporter family, it does not transport sugars.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.