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Protein

Inorganic phosphate transporter 1-1

Gene

PHT1-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

High-affinity transporter for external inorganic phosphate. Acts as a H+:phosphate symporter in both low- and high-Pi conditions. Confers sensitivity to arsenate.3 Publications

Miscellaneous

Although related to the sugar transporter family, it does not transport sugars.

Enzyme regulationi

Inhibited by protonophores (e.g. 2,4-dinitrophenol and carbonylcyanide m-chlorophenylhydrazone), the plasma membrane H+-ATPase inhibitor diethylstilbestorol, and the phosphate analog arsenate.1 Publication

Kineticsi

  1. KM=3 µM for inorganic phosphate1 Publication

    GO - Molecular functioni

    • arsenate ion transmembrane transporter activity Source: TAIR
    • inorganic phosphate transmembrane transporter activity Source: TAIR
    • phosphate ion transmembrane transporter activity Source: TAIR
    • symporter activity Source: UniProtKB-KW

    GO - Biological processi

    • arsenate ion transmembrane transport Source: TAIR
    • cellular response to phosphate starvation Source: TAIR
    • phosphate ion transport Source: TAIR

    Keywordsi

    Biological processPhosphate transport, Symport, Transport

    Enzyme and pathway databases

    BioCyciARA:GQT-2848-MONOMER

    Protein family/group databases

    TCDBi2.A.1.9.9 the major facilitator superfamily (mfs)

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inorganic phosphate transporter 1-1
    Short name:
    AtPht1;1
    Alternative name(s):
    H(+)/Pi cotransporter
    Gene namesi
    Name:PHT1-1
    Synonyms:APT2, PHT1, PT1
    Ordered Locus Names:At5g43350
    ORF Names:MWF20.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    AraportiAT5G43350
    TAIRilocus:2176461 AT5G43350

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 24CytoplasmicSequence analysisAdd BLAST24
    Transmembranei25 – 45HelicalSequence analysisAdd BLAST21
    Topological domaini46 – 70ExtracellularSequence analysisAdd BLAST25
    Transmembranei71 – 91HelicalSequence analysisAdd BLAST21
    Topological domaini92 – 99CytoplasmicSequence analysis8
    Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
    Topological domaini121 – 131ExtracellularSequence analysisAdd BLAST11
    Transmembranei132 – 152HelicalSequence analysisAdd BLAST21
    Topological domaini153 – 161CytoplasmicSequence analysis9
    Transmembranei162 – 182HelicalSequence analysisAdd BLAST21
    Topological domaini183 – 211ExtracellularSequence analysisAdd BLAST29
    Transmembranei212 – 232HelicalSequence analysisAdd BLAST21
    Topological domaini233 – 292CytoplasmicSequence analysisAdd BLAST60
    Transmembranei293 – 313HelicalSequence analysisAdd BLAST21
    Topological domaini314 – 348ExtracellularSequence analysisAdd BLAST35
    Transmembranei349 – 369HelicalSequence analysisAdd BLAST21
    Topological domaini370 – 371CytoplasmicSequence analysis2
    Transmembranei372 – 392HelicalSequence analysisAdd BLAST21
    Topological domaini393 – 402ExtracellularSequence analysis10
    Transmembranei403 – 423HelicalSequence analysisAdd BLAST21
    Topological domaini424 – 441CytoplasmicSequence analysisAdd BLAST18
    Transmembranei442 – 462HelicalSequence analysisAdd BLAST21
    Topological domaini463 – 484ExtracellularSequence analysisAdd BLAST22
    Transmembranei485 – 505HelicalSequence analysisAdd BLAST21
    Topological domaini506 – 524CytoplasmicSequence analysisAdd BLAST19

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000504681 – 524Inorganic phosphate transporter 1-1Add BLAST524

    Proteomic databases

    PaxDbiQ8VYM2
    PRIDEiQ8VYM2

    PTM databases

    iPTMnetiQ8VYM2

    Expressioni

    Tissue specificityi

    Mostly expressed in roots, especially in trichoblasts and in emerging secondary roots and root hairs, but not in root tips. Also present in hydathodes, axillary buds and peripheral endosperm of germinating seeds.6 Publications

    Inductioni

    In roots by phosphate starvation. Repressed by auxin, cytokinins, and the Pi analog phosphite (Phi).7 Publications

    Gene expression databases

    ExpressionAtlasiQ8VYM2 baseline and differential
    GenevisibleiQ8VYM2 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi19603, 5 interactors
    STRINGi3702.AT5G43350.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VYM2
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG0252 Eukaryota
    ENOG410ZVN7 LUCA
    HOGENOMiHOG000171120
    InParanoidiQ8VYM2
    KOiK08176
    OMAiIFASIHW
    OrthoDBiEOG093607P7
    PhylomeDBiQ8VYM2

    Family and domain databases

    CDDicd06174 MFS, 1 hit
    InterProiView protein in InterPro
    IPR020846 MFS_dom
    IPR005828 MFS_sugar_transport-like
    IPR036259 MFS_trans_sf
    IPR004738 Phos_permease
    PfamiView protein in Pfam
    PF00083 Sugar_tr, 1 hit
    SUPFAMiSSF103473 SSF103473, 1 hit
    TIGRFAMsiTIGR00887 2A0109, 1 hit
    PROSITEiView protein in PROSITE
    PS50850 MFS, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q8VYM2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAEQQLGVLK ALDVAKTQLY HFTAIVIAGM GFFTDAYDLF CVSLVTKLLG
    60 70 80 90 100
    RIYYFNPESA KPGSLPPHVA AAVNGVALCG TLSGQLFFGW LGDKLGRKKV
    110 120 130 140 150
    YGLTLVMMIL CSVASGLSFG HEAKGVMTTL CFFRFWLGFG IGGDYPLSAT
    160 170 180 190 200
    IMSEYANKKT RGAFIAAVFA MQGVGILAGG FVALAVSSIF DKKFPAPTYA
    210 220 230 240 250
    VNRALSTPPQ VDYIWRIIVM FGALPAALTY YWRMKMPETA RYTALVAKNI
    260 270 280 290 300
    KQATADMSKV LQTDIELEER VEDDVKDPKQ NYGLFSKEFL RRHGLHLLGT
    310 320 330 340 350
    TSTWFLLDIA FYSQNLFQKD IFSAIGWIPK AATMNATHEV FRIARAQTLI
    360 370 380 390 400
    ALCSTVPGYW FTVAFIDTIG RFKIQLNGFF MMTVFMFAIA FPYNHWIKPE
    410 420 430 440 450
    NRIGFVVMYS LTFFFANFGP NATTFIVPAE IFPARLRSTC HGISAAAGKA
    460 470 480 490 500
    GAIVGAFGFL YAAQSQDKAK VDAGYPPGIG VKNSLIMLGV LNFIGMLFTF
    510 520
    LVPEPKGKSL EELSGEAEVS HDEK
    Length:524
    Mass (Da):57,616
    Last modified:January 10, 2006 - v2
    Checksum:i2D12D50987127BD6
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti185A → T in AAL49927 (PubMed:14593172).Curated1
    Sequence conflicti469A → P in CAA68946 (PubMed:9025304).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U62330 mRNA Translation: AAB17265.1
    Y07682 Genomic DNA Translation: CAA68946.1
    D86591 mRNA Translation: BAA21503.1
    D86608 Genomic DNA Translation: BAA21504.1
    AB025638 Genomic DNA Translation: BAA97414.1
    CP002688 Genomic DNA Translation: AED94948.1
    AY070432 mRNA Translation: AAL49927.1
    RefSeqiNP_199149.1, NM_123701.4
    UniGeneiAt.49125
    At.75586

    Genome annotation databases

    EnsemblPlantsiAT5G43350.1; AT5G43350.1; AT5G43350
    GeneIDi834353
    GrameneiAT5G43350.1; AT5G43350.1; AT5G43350
    KEGGiath:AT5G43350

    Similar proteinsi

    Entry informationi

    Entry nameiPHT11_ARATH
    AccessioniPrimary (citable) accession number: Q8VYM2
    Secondary accession number(s): Q96264, Q96302
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: January 10, 2006
    Last modified: April 25, 2018
    This is version 119 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families
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