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Protein

Inorganic phosphate transporter 1-1

Gene

PHT1-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity transporter for external inorganic phosphate. Acts as a H+:phosphate symporter in both low- and high-Pi conditions. Confers sensitivity to arsenate.3 Publications

Enzyme regulationi

Inhibited by protonophores (e.g. 2,4-dinitrophenol and carbonylcyanide m-chlorophenylhydrazone), the plasma membrane H+-ATPase inhibitor diethylstilbestorol, and the phosphate analog arsenate.1 Publication

Kineticsi

  1. KM=3 µM for inorganic phosphate1 Publication

    GO - Molecular functioni

    • arsenate ion transmembrane transporter activity Source: TAIR
    • inorganic phosphate transmembrane transporter activity Source: TAIR
    • phosphate ion transmembrane transporter activity Source: TAIR
    • symporter activity Source: UniProtKB-KW

    GO - Biological processi

    • arsenate ion transmembrane transport Source: TAIR
    • cellular response to phosphate starvation Source: TAIR
    • phosphate ion transport Source: TAIR
    Complete GO annotation...

    Keywords - Biological processi

    Phosphate transport, Symport, Transport

    Enzyme and pathway databases

    BioCyciARA:GQT-2848-MONOMER.

    Protein family/group databases

    TCDBi2.A.1.9.9. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inorganic phosphate transporter 1-1
    Short name:
    AtPht1;1
    Alternative name(s):
    H(+)/Pi cotransporter
    Gene namesi
    Name:PHT1-1
    Synonyms:APT2, PHT1, PT1
    Ordered Locus Names:At5g43350
    ORF Names:MWF20.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    TAIRiAT5G43350.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 24CytoplasmicSequence analysisAdd BLAST24
    Transmembranei25 – 45HelicalSequence analysisAdd BLAST21
    Topological domaini46 – 70ExtracellularSequence analysisAdd BLAST25
    Transmembranei71 – 91HelicalSequence analysisAdd BLAST21
    Topological domaini92 – 99CytoplasmicSequence analysis8
    Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
    Topological domaini121 – 131ExtracellularSequence analysisAdd BLAST11
    Transmembranei132 – 152HelicalSequence analysisAdd BLAST21
    Topological domaini153 – 161CytoplasmicSequence analysis9
    Transmembranei162 – 182HelicalSequence analysisAdd BLAST21
    Topological domaini183 – 211ExtracellularSequence analysisAdd BLAST29
    Transmembranei212 – 232HelicalSequence analysisAdd BLAST21
    Topological domaini233 – 292CytoplasmicSequence analysisAdd BLAST60
    Transmembranei293 – 313HelicalSequence analysisAdd BLAST21
    Topological domaini314 – 348ExtracellularSequence analysisAdd BLAST35
    Transmembranei349 – 369HelicalSequence analysisAdd BLAST21
    Topological domaini370 – 371CytoplasmicSequence analysis2
    Transmembranei372 – 392HelicalSequence analysisAdd BLAST21
    Topological domaini393 – 402ExtracellularSequence analysis10
    Transmembranei403 – 423HelicalSequence analysisAdd BLAST21
    Topological domaini424 – 441CytoplasmicSequence analysisAdd BLAST18
    Transmembranei442 – 462HelicalSequence analysisAdd BLAST21
    Topological domaini463 – 484ExtracellularSequence analysisAdd BLAST22
    Transmembranei485 – 505HelicalSequence analysisAdd BLAST21
    Topological domaini506 – 524CytoplasmicSequence analysisAdd BLAST19

    GO - Cellular componenti

    • integral component of plasma membrane Source: GO_Central
    • plasma membrane Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000504681 – 524Inorganic phosphate transporter 1-1Add BLAST524

    Proteomic databases

    PaxDbiQ8VYM2.
    PRIDEiQ8VYM2.

    PTM databases

    iPTMnetiQ8VYM2.

    Expressioni

    Tissue specificityi

    Mostly expressed in roots, especially in trichoblasts and in emerging secondary roots and root hairs, but not in root tips. Also present in hydathodes, axillary buds and peripheral endosperm of germinating seeds.6 Publications

    Inductioni

    In roots by phosphate starvation. Repressed by auxin, cytokinins, and the Pi analog phosphite (Phi).7 Publications

    Gene expression databases

    GenevisibleiQ8VYM2. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi19603. 5 interactors.
    MINTiMINT-8071073.
    STRINGi3702.AT5G43350.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VYM2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG0252. Eukaryota.
    ENOG410ZVN7. LUCA.
    HOGENOMiHOG000171120.
    InParanoidiQ8VYM2.
    KOiK08176.
    OMAiQMANIGG.
    OrthoDBiEOG093607P7.
    PhylomeDBiQ8VYM2.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR020846. MFS_dom.
    IPR005828. MFS_sugar_transport-like.
    IPR004738. Phos_permease.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00887. 2A0109. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8VYM2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAEQQLGVLK ALDVAKTQLY HFTAIVIAGM GFFTDAYDLF CVSLVTKLLG
    60 70 80 90 100
    RIYYFNPESA KPGSLPPHVA AAVNGVALCG TLSGQLFFGW LGDKLGRKKV
    110 120 130 140 150
    YGLTLVMMIL CSVASGLSFG HEAKGVMTTL CFFRFWLGFG IGGDYPLSAT
    160 170 180 190 200
    IMSEYANKKT RGAFIAAVFA MQGVGILAGG FVALAVSSIF DKKFPAPTYA
    210 220 230 240 250
    VNRALSTPPQ VDYIWRIIVM FGALPAALTY YWRMKMPETA RYTALVAKNI
    260 270 280 290 300
    KQATADMSKV LQTDIELEER VEDDVKDPKQ NYGLFSKEFL RRHGLHLLGT
    310 320 330 340 350
    TSTWFLLDIA FYSQNLFQKD IFSAIGWIPK AATMNATHEV FRIARAQTLI
    360 370 380 390 400
    ALCSTVPGYW FTVAFIDTIG RFKIQLNGFF MMTVFMFAIA FPYNHWIKPE
    410 420 430 440 450
    NRIGFVVMYS LTFFFANFGP NATTFIVPAE IFPARLRSTC HGISAAAGKA
    460 470 480 490 500
    GAIVGAFGFL YAAQSQDKAK VDAGYPPGIG VKNSLIMLGV LNFIGMLFTF
    510 520
    LVPEPKGKSL EELSGEAEVS HDEK
    Length:524
    Mass (Da):57,616
    Last modified:January 10, 2006 - v2
    Checksum:i2D12D50987127BD6
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti185A → T in AAL49927 (PubMed:14593172).Curated1
    Sequence conflicti469A → P in CAA68946 (PubMed:9025304).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U62330 mRNA. Translation: AAB17265.1.
    Y07682 Genomic DNA. Translation: CAA68946.1.
    D86591 mRNA. Translation: BAA21503.1.
    D86608 Genomic DNA. Translation: BAA21504.1.
    AB025638 Genomic DNA. Translation: BAA97414.1.
    CP002688 Genomic DNA. Translation: AED94948.1.
    AY070432 mRNA. Translation: AAL49927.1.
    RefSeqiNP_199149.1. NM_123701.4.
    UniGeneiAt.49125.
    At.75586.

    Genome annotation databases

    EnsemblPlantsiAT5G43350.1; AT5G43350.1; AT5G43350.
    GeneIDi834353.
    GrameneiAT5G43350.1; AT5G43350.1; AT5G43350.
    KEGGiath:AT5G43350.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U62330 mRNA. Translation: AAB17265.1.
    Y07682 Genomic DNA. Translation: CAA68946.1.
    D86591 mRNA. Translation: BAA21503.1.
    D86608 Genomic DNA. Translation: BAA21504.1.
    AB025638 Genomic DNA. Translation: BAA97414.1.
    CP002688 Genomic DNA. Translation: AED94948.1.
    AY070432 mRNA. Translation: AAL49927.1.
    RefSeqiNP_199149.1. NM_123701.4.
    UniGeneiAt.49125.
    At.75586.

    3D structure databases

    ProteinModelPortaliQ8VYM2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi19603. 5 interactors.
    MINTiMINT-8071073.
    STRINGi3702.AT5G43350.1.

    Protein family/group databases

    TCDBi2.A.1.9.9. the major facilitator superfamily (mfs).

    PTM databases

    iPTMnetiQ8VYM2.

    Proteomic databases

    PaxDbiQ8VYM2.
    PRIDEiQ8VYM2.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G43350.1; AT5G43350.1; AT5G43350.
    GeneIDi834353.
    GrameneiAT5G43350.1; AT5G43350.1; AT5G43350.
    KEGGiath:AT5G43350.

    Organism-specific databases

    TAIRiAT5G43350.

    Phylogenomic databases

    eggNOGiKOG0252. Eukaryota.
    ENOG410ZVN7. LUCA.
    HOGENOMiHOG000171120.
    InParanoidiQ8VYM2.
    KOiK08176.
    OMAiQMANIGG.
    OrthoDBiEOG093607P7.
    PhylomeDBiQ8VYM2.

    Enzyme and pathway databases

    BioCyciARA:GQT-2848-MONOMER.

    Miscellaneous databases

    PROiQ8VYM2.

    Gene expression databases

    GenevisibleiQ8VYM2. AT.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR020846. MFS_dom.
    IPR005828. MFS_sugar_transport-like.
    IPR004738. Phos_permease.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00887. 2A0109. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPHT11_ARATH
    AccessioniPrimary (citable) accession number: Q8VYM2
    Secondary accession number(s): Q96264, Q96302
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: January 10, 2006
    Last modified: November 30, 2016
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Although related to the sugar transporter family, it does not transport sugars.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.