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Protein

Aspartic proteinase A2

Gene

APA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the breakdown of propeptides of storage proteins in protein-storage vacuoles.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei107PROSITE-ProRule annotation1
Active sitei294PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

ReactomeiR-ATH-2132295. MHC class II antigen presentation.
R-ATH-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiA01.A02.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartic proteinase A2 (EC:3.4.23.-)
Alternative name(s):
Aspartic protease 57
Short name:
AtASP57
Gene namesi
Name:APA2
Ordered Locus Names:At1g62290
ORF Names:F19K23.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G62290.

Subcellular locationi

GO - Cellular componenti

  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000042062725 – 71Activation peptideSequence analysisAdd BLAST47
ChainiPRO_000042062872 – 513Aspartic proteinase A2Add BLAST442

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi120 ↔ 126PROSITE-ProRule annotation
Disulfide bondi285 ↔ 289PROSITE-ProRule annotation
Disulfide bondi324 ↔ 418PROSITE-ProRule annotation
Disulfide bondi349 ↔ 390PROSITE-ProRule annotation
Disulfide bondi355 ↔ 387PROSITE-ProRule annotation
Glycosylationi404N-linked (GlcNAc...)PROSITE-ProRule annotation1
Disulfide bondi432 ↔ 469PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ8VYL3.
PRIDEiQ8VYL3.

PTM databases

iPTMnetiQ8VYL3.

Expressioni

Tissue specificityi

Expressed in seed pods and dry seeds.1 Publication

Gene expression databases

GenevisibleiQ8VYL3. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G62290.1.

Structurei

3D structure databases

ProteinModelPortaliQ8VYL3.
SMRiQ8VYL3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini89 – 510Peptidase A1PROSITE-ProRule annotationAdd BLAST422
Domaini319 – 424Saposin B-typePROSITE-ProRule annotationAdd BLAST106

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation
Contains 1 saposin B-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOGENOMiHOG000197681.
InParanoidiQ8VYL3.
KOiK08245.
OMAiDICERMP.
OrthoDBiEOG093606Q0.
PhylomeDBiQ8VYL3.

Family and domain databases

CDDicd06098. phytepsin. 1 hit.
Gene3Di1.10.225.10. 2 hits.
2.40.70.10. 3 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR033869. Phytepsin.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 2 hits.
PfamiPF00026. Asp. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SMARTiSM00741. SapB. 2 hits.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 1 hit.
SSF50630. SSF50630. 2 hits.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
PS50015. SAP_B. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VYL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVYSRAVAF SVFVSFLLFF TAYSKRNDGT FRVGLKKLKL DPNNRLATRF
60 70 80 90 100
GSKQEEALRS SLRSYNNNLG GDSGDADIVP LKNYLDAQYY GEIAIGTPPQ
110 120 130 140 150
KFTVIFDTGS SNLWVPSGKC FFSLSCYFHA KYKSSRSSTY KKSGKRAAIH
160 170 180 190 200
YGSGSISGFF SYDAVTVGDL VVKDQEFIET TSEPGLTFLV AKFDGLLGLG
210 220 230 240 250
FQEIAVGNAT PVWYNMLKQG LIKRPVFSFW LNRDPKSEEG GEIVFGGVDP
260 270 280 290 300
KHFRGEHTFV PVTQRGYWQF DMGEVLIAGE STGYCGSGCS AIADSGTSLL
310 320 330 340 350
AGPTAVVAMI NKAIGASGVV SQQCKTVVDQ YGQTILDLLL AETQPKKICS
360 370 380 390 400
QIGLCAYDGT HGVSMGIESV VDKENTRSSS GLRDAGCPAC EMAVVWIQSQ
410 420 430 440 450
LRQNMTQERI VNYINEICER MPSPNGESAV DCSQLSKMPT VSFTIGGKVF
460 470 480 490 500
DLAPEEYVLK IGEGPVAQCI SGFTALDIPP PRGPLWILGD VFMGKYHTVF
510
DFGNEQVGFA EAV
Length:513
Mass (Da):55,748
Last modified:March 1, 2002 - v1
Checksum:iD449D42CEB6A7441
GO

Sequence cautioni

The sequence AAB60773 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC000375 Genomic DNA. Translation: AAB60773.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33946.1.
CP002684 Genomic DNA. Translation: AEE33947.1.
AY070453 mRNA. Translation: AAL49856.1.
AY142687 mRNA. Translation: AAN13225.1.
AK317285 mRNA. Translation: BAH19961.1.
PIRiE96649.
RefSeqiNP_001031219.1. NM_001036142.3.
NP_176419.2. NM_104909.5.
UniGeneiAt.23367.

Genome annotation databases

EnsemblPlantsiAT1G62290.1; AT1G62290.1; AT1G62290.
AT1G62290.2; AT1G62290.2; AT1G62290.
GeneIDi842526.
GrameneiAT1G62290.1; AT1G62290.1; AT1G62290.
AT1G62290.2; AT1G62290.2; AT1G62290.
KEGGiath:AT1G62290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC000375 Genomic DNA. Translation: AAB60773.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33946.1.
CP002684 Genomic DNA. Translation: AEE33947.1.
AY070453 mRNA. Translation: AAL49856.1.
AY142687 mRNA. Translation: AAN13225.1.
AK317285 mRNA. Translation: BAH19961.1.
PIRiE96649.
RefSeqiNP_001031219.1. NM_001036142.3.
NP_176419.2. NM_104909.5.
UniGeneiAt.23367.

3D structure databases

ProteinModelPortaliQ8VYL3.
SMRiQ8VYL3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G62290.1.

Protein family/group databases

MEROPSiA01.A02.

PTM databases

iPTMnetiQ8VYL3.

Proteomic databases

PaxDbiQ8VYL3.
PRIDEiQ8VYL3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G62290.1; AT1G62290.1; AT1G62290.
AT1G62290.2; AT1G62290.2; AT1G62290.
GeneIDi842526.
GrameneiAT1G62290.1; AT1G62290.1; AT1G62290.
AT1G62290.2; AT1G62290.2; AT1G62290.
KEGGiath:AT1G62290.

Organism-specific databases

TAIRiAT1G62290.

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOGENOMiHOG000197681.
InParanoidiQ8VYL3.
KOiK08245.
OMAiDICERMP.
OrthoDBiEOG093606Q0.
PhylomeDBiQ8VYL3.

Enzyme and pathway databases

ReactomeiR-ATH-2132295. MHC class II antigen presentation.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ8VYL3.

Gene expression databases

GenevisibleiQ8VYL3. AT.

Family and domain databases

CDDicd06098. phytepsin. 1 hit.
Gene3Di1.10.225.10. 2 hits.
2.40.70.10. 3 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR033869. Phytepsin.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 2 hits.
PfamiPF00026. Asp. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SMARTiSM00741. SapB. 2 hits.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 1 hit.
SSF50630. SSF50630. 2 hits.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
PS50015. SAP_B. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPA2_ARATH
AccessioniPrimary (citable) accession number: Q8VYL3
Secondary accession number(s): O04593
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.