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Q8VYE5

- E1312_ARATH

UniProt

Q8VYE5 - E1312_ARATH

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Protein

Glucan endo-1,3-beta-glucosidase 12

Gene

At4g29360

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei264 – 2641NucleophileBy similarity
Active sitei327 – 3271NucleophileBy similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cell wall organization Source: UniProtKB-KW
  3. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Plant defense

Enzyme and pathway databases

BioCyciARA:AT4G29360-MONOMER.
ARA:GQT-165-MONOMER.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase 12 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase 12
Short name:
(1->3)-beta-glucanase 12
Beta-1,3-endoglucanase 12
Short name:
Beta-1,3-glucanase 12
Gene namesi
Ordered Locus Names:At4g29360
ORF Names:F17A13.180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G29360.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: TAIR
  2. anchored component of plasma membrane Source: TAIR
  3. cell wall Source: UniProtKB-KW
  4. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 507483Glucan endo-1,3-beta-glucosidase 12PRO_0000251271Add
BLAST
Propeptidei508 – 53427Removed in mature formSequence AnalysisPRO_0000251272Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi127 – 1271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi336 – 3361N-linked (GlcNAc...)Sequence Analysis
Glycosylationi357 – 3571N-linked (GlcNAc...)Sequence Analysis
Glycosylationi375 – 3751N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi392 ↔ 455By similarity
Glycosylationi485 – 4851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi491 – 4911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi495 – 4951N-linked (GlcNAc...)Sequence Analysis
Lipidationi507 – 5071GPI-anchor amidated serineSequence Analysis

Post-translational modificationi

Contains two additional disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ8VYE5.
PRIDEiQ8VYE5.

Expressioni

Gene expression databases

ExpressionAtlasiQ8VYE5. baseline and differential.
GenevestigatoriQ8VYE5.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G29360.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8VYE5.
SMRiQ8VYE5. Positions 27-345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi381 – 3877Poly-Gly

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG322041.
HOGENOMiHOG000238220.
InParanoidiQ8VYE5.
OMAiKWCIASS.
PhylomeDBiQ8VYE5.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8VYE5) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQRLNLVFW IFVSILAFLN FGMASKIGIC YGRNADNLPS PNRVSELIQH
60 70 80 90 100
LNIKFVRIYD ANIDVLKAFA NTGIELMIGV PNADLLAFAQ FQSNVDTWLS
110 120 130 140 150
NNILPYYPST KITSISVGLE VTEAPDNATG LVLPAMRNIH TALKKSGLDK
160 170 180 190 200
KIKISSSHSL AILSRSFPPS SASFSKKHSA FLKPMLEFLV ENESPFMIDL
210 220 230 240 250
YPYYAYRDST EKVPLEYALF ESSSQVVDPA TGLLYSNMFD AQLDAIYFAL
260 270 280 290 300
TAMSFKTVKV MVTESGWPSK GSPKETAATP ENALAYNTNL IRHVIGDPGT
310 320 330 340 350
PAKPGEEIDV YLFSLFNENR KPGIESERNW GMFYANGTNV YALDFTGENT
360 370 380 390 400
TPVSPTNSTT GTSPSPSSSP IINGNSTVTI GGGGGGGTKK WCIASSQASV
410 420 430 440 450
TELQTALDWA CGPGNVDCSA VQPDQPCFEP DTVLSHASYA FNTYYQQSGA
460 470 480 490 500
SSIDCSFNGA SVEVDKDPSY GNCLYMIAPA TDGFNRTMAG NITGNITAID
510 520 530
SPLASPSSTN EAFRQMVVAV SVLLPCFVVC SSIW
Length:534
Mass (Da):57,659
Last modified:March 1, 2002 - v1
Checksum:iD76C3E1759D4CA59
GO
Isoform 2 (identifier: Q8VYE5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     470-494: YGNCLYMIAPATDGFNRTMAGNITG → KYLYTYTFQNTSIKSNVKP

Note: May be due to an intron retention. No experimental confirmation available.

Show »
Length:528
Mass (Da):57,303
Checksum:i05558FC6F457FFD3
GO

Sequence cautioni

The sequence CAB79694.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei470 – 49425YGNCL…GNITG → KYLYTYTFQNTSIKSNVKP in isoform 2. 1 PublicationVSP_020753Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL161574 Genomic DNA. Translation: CAB79694.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85622.1.
AY072133 mRNA. Translation: AAL59955.1.
AY096465 mRNA. Translation: AAM20105.1.
AY088354 mRNA. Translation: AAM65893.1.
PIRiF85342.
RefSeqiNP_567828.3. NM_119081.5.
NP_849556.1. NM_179225.3. [Q8VYE5-1]
UniGeneiAt.31933.

Genome annotation databases

EnsemblPlantsiAT4G29360.1; AT4G29360.1; AT4G29360. [Q8VYE5-1]
GeneIDi829057.
KEGGiath:AT4G29360.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL161574 Genomic DNA. Translation: CAB79694.1 . Sequence problems.
CP002687 Genomic DNA. Translation: AEE85622.1 .
AY072133 mRNA. Translation: AAL59955.1 .
AY096465 mRNA. Translation: AAM20105.1 .
AY088354 mRNA. Translation: AAM65893.1 .
PIRi F85342.
RefSeqi NP_567828.3. NM_119081.5.
NP_849556.1. NM_179225.3. [Q8VYE5-1 ]
UniGenei At.31933.

3D structure databases

ProteinModelPortali Q8VYE5.
SMRi Q8VYE5. Positions 27-345.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT4G29360.1-P.

Protein family/group databases

CAZyi CBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbi Q8VYE5.
PRIDEi Q8VYE5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G29360.1 ; AT4G29360.1 ; AT4G29360 . [Q8VYE5-1 ]
GeneIDi 829057.
KEGGi ath:AT4G29360.

Organism-specific databases

TAIRi AT4G29360.

Phylogenomic databases

eggNOGi NOG322041.
HOGENOMi HOG000238220.
InParanoidi Q8VYE5.
OMAi KWCIASS.
PhylomeDBi Q8VYE5.

Enzyme and pathway databases

BioCyci ARA:AT4G29360-MONOMER.
ARA:GQT-165-MONOMER.

Gene expression databases

ExpressionAtlasi Q8VYE5. baseline and differential.
Genevestigatori Q8VYE5.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view ]
Pfami PF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view ]
SMARTi SM00768. X8. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  5. "Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
    Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
    Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiE1312_ARATH
AccessioniPrimary (citable) accession number: Q8VYE5
Secondary accession number(s): Q8L9M3, Q9M0E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: March 1, 2002
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3