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Protein

U11/U12 small nuclear ribonucleoprotein 35 kDa protein

Gene

SNRNP35

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May facilitate 5' splice site recognition in the minor spliceosome. May be involved in interactions with components of the major spliceosome bound to the pyrimidine tract of an upstream U2-type intron.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-ATH-72165. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
U11/U12 small nuclear ribonucleoprotein 35 kDa protein
Short name:
U11/U12 snRNP 35 kDa protein
Short name:
U11/U12-35K
Gene namesi
Name:SNRNP35
Ordered Locus Names:At2g43370
ORF Names:T1O24.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G43370.
TAIRilocus:2058141. AT2G43370.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004298301 – 333U11/U12 small nuclear ribonucleoprotein 35 kDa proteinAdd BLAST333

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8VY74.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

ExpressionAtlasiQ8VY74. baseline and differential.
GenevisibleiQ8VY74. AT.

Interactioni

Subunit structurei

Part of the U11 snRNP, a component of the minor U12-type spliceosome. U11 and U12 snRNPs exist not only as monoparticles but also as a preformed U11/U12 di-snRNP complex. Interacts with CYP95, SCL28, SCL30, SCL30A, SCL33, SC35, SR30, SR34, RS31, RS40, RSZ21 and RS2Z33.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SCL30Q8L3X82EBI-927038,EBI-927061

Protein-protein interaction databases

BioGridi4275. 29 interactors.
IntActiQ8VY74. 31 interactors.
STRINGi3702.AT2G43370.1.

Structurei

3D structure databases

ProteinModelPortaliQ8VY74.
SMRiQ8VY74.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini64 – 142RRMPROSITE-ProRule annotationAdd BLAST79

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi259 – 323Arg-richAdd BLAST65
Compositional biasi313 – 330His-richAdd BLAST18

Phylogenomic databases

eggNOGiKOG0113. Eukaryota.
COG0724. LUCA.
HOGENOMiHOG000006374.
InParanoidiQ8VY74.
KOiK13155.
OMAiLSHHTTE.
OrthoDBiEOG09360HMJ.
PhylomeDBiQ8VY74.

Family and domain databases

CDDicd12237. RRM_snRNP35. 1 hit.
InterProiView protein in InterPro
IPR035979. RBD_domain_sf.
IPR000504. RRM_dom.
IPR034146. snRNP35_RRM.
PfamiView protein in Pfam
PF00076. RRM_1. 1 hit.
SMARTiView protein in SMART
SM00360. RRM. 1 hit.
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiView protein in PROSITE
PS50102. RRM. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VY74-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGGGNNVVN KVFYATSYHP IQAGSIDGTD VAPHDNGVRR ALLCYNAGLY
60 70 80 90 100
DPSGDSKAVG DPYCTLFVGR LSHHTTEDTL REVMSKYGRI KNLRLVRHIV
110 120 130 140 150
TGASRGYGFV EYETEKEMLR AYEDAHHSLI DGREIIVDYN RQQLMPGWIP
160 170 180 190 200
RRLGGGLGGR KESGQLRFGG RDRPFRAPLR PIPHEDLKKL GIQLPPEGRY
210 220 230 240 250
MSRTQIPSPP RRKGSVSDRE EEYYREKSSV EREEEFKERS SLRSYHSHRS
260 270 280 290 300
SAHTHSSHRR RSKDREECSR EESRSDRKER ARGMEDRYGD NKGEVSGSKR
310 320 330
SKRSEEDRSR KRHKHLPSHH HRRSYSQDHH SSD
Length:333
Mass (Da):38,375
Last modified:March 1, 2002 - v1
Checksum:i11FE374575F6C137
GO
Isoform 2 (identifier: Q8VY74-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     123-123: E → EQ

Note: Has no effect on the assembly of the protein into snRNP, but reduces the affinity for SR proteins.
Show »
Length:334
Mass (Da):38,504
Checksum:i0A2C5215CBF925D7
GO
Isoform 3 (identifier: Q8VY74-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     180-187: RPIPHEDL → YGFALLFL
     188-333: Missing.

Show »
Length:187
Mass (Da):20,837
Checksum:iFEB17904E4BE622C
GO

Sequence cautioni

The sequence AAB64330 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAC43032 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti214G → R in BAC43032 (PubMed:11910074).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055300123E → EQ in isoform 2. 1 Publication1
Alternative sequenceiVSP_055301180 – 187RPIPHEDL → YGFALLFL in isoform 3. 1 Publication8
Alternative sequenceiVSP_055302188 – 333Missing in isoform 3. 1 PublicationAdd BLAST146

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002335 Genomic DNA. Translation: AAB64330.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10257.1.
AY072388 mRNA. Translation: AAL62380.1.
AY114697 mRNA. Translation: AAM48016.1.
AK118423 mRNA. Translation: BAC43032.1. Different initiation.
PIRiC84865.
RefSeqiNP_850395.1. NM_180064.3. [Q8VY74-1]
UniGeneiAt.42685.

Genome annotation databases

EnsemblPlantsiAT2G43370.1; AT2G43370.1; AT2G43370. [Q8VY74-1]
GeneIDi818938.
GrameneiAT2G43370.1; AT2G43370.1; AT2G43370.
KEGGiath:AT2G43370.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiU1135_ARATH
AccessioniPrimary (citable) accession number: Q8VY74
Secondary accession number(s): O22851, Q8GX59
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: March 1, 2002
Last modified: October 25, 2017
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names