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Protein

U11/U12 small nuclear ribonucleoprotein 35 kDa protein

Gene

SNRNP35

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May facilitate 5' splice site recognition in the minor spliceosome. May be involved in interactions with components of the major spliceosome bound to the pyrimidine tract of an upstream U2-type intron.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-ATH-72165. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
U11/U12 small nuclear ribonucleoprotein 35 kDa protein
Short name:
U11/U12 snRNP 35 kDa protein
Short name:
U11/U12-35K
Gene namesi
Name:SNRNP35
Ordered Locus Names:At2g43370
ORF Names:T1O24.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G43370.

Subcellular locationi

  • Nucleus speckle 1 Publication

GO - Cellular componenti

  • nuclear speck Source: UniProtKB-SubCell
  • nucleus Source: GO_Central
  • spliceosomal complex Source: UniProtKB-KW
  • U11/U12 snRNP Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 333333U11/U12 small nuclear ribonucleoprotein 35 kDa proteinPRO_0000429830Add
BLAST

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8VY74.
PRIDEiQ8VY74.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

GenevisibleiQ8VY74. AT.

Interactioni

Subunit structurei

Part of the U11 snRNP, a component of the minor U12-type spliceosome. U11 and U12 snRNPs exist not only as monoparticles but also as a preformed U11/U12 di-snRNP complex. Interacts with CYP95, SCL28, SCL30, SCL30A, SCL33, SC35, SR30, SR34, RS31, RS40, RSZ21 and RS2Z33.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SCL30Q8L3X82EBI-927038,EBI-927061

Protein-protein interaction databases

BioGridi4275. 29 interactions.
IntActiQ8VY74. 31 interactions.
STRINGi3702.AT2G43370.1.

Structurei

3D structure databases

ProteinModelPortaliQ8VY74.
SMRiQ8VY74. Positions 49-152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini64 – 14279RRMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi259 – 32365Arg-richAdd
BLAST
Compositional biasi313 – 33018His-richAdd
BLAST

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0113. Eukaryota.
COG0724. LUCA.
HOGENOMiHOG000006374.
InParanoidiQ8VY74.
KOiK13155.
OMAiCYNAGLY.
PhylomeDBiQ8VY74.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VY74-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGGGNNVVN KVFYATSYHP IQAGSIDGTD VAPHDNGVRR ALLCYNAGLY
60 70 80 90 100
DPSGDSKAVG DPYCTLFVGR LSHHTTEDTL REVMSKYGRI KNLRLVRHIV
110 120 130 140 150
TGASRGYGFV EYETEKEMLR AYEDAHHSLI DGREIIVDYN RQQLMPGWIP
160 170 180 190 200
RRLGGGLGGR KESGQLRFGG RDRPFRAPLR PIPHEDLKKL GIQLPPEGRY
210 220 230 240 250
MSRTQIPSPP RRKGSVSDRE EEYYREKSSV EREEEFKERS SLRSYHSHRS
260 270 280 290 300
SAHTHSSHRR RSKDREECSR EESRSDRKER ARGMEDRYGD NKGEVSGSKR
310 320 330
SKRSEEDRSR KRHKHLPSHH HRRSYSQDHH SSD
Length:333
Mass (Da):38,375
Last modified:March 1, 2002 - v1
Checksum:i11FE374575F6C137
GO
Isoform 2 (identifier: Q8VY74-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     123-123: E → EQ

Note: Has no effect on the assembly of the protein into snRNP, but reduces the affinity for SR proteins.
Show »
Length:334
Mass (Da):38,504
Checksum:i0A2C5215CBF925D7
GO
Isoform 3 (identifier: Q8VY74-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     180-187: RPIPHEDL → YGFALLFL
     188-333: Missing.

Show »
Length:187
Mass (Da):20,837
Checksum:iFEB17904E4BE622C
GO

Sequence cautioni

The sequence AAB64330.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence BAC43032.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti214 – 2141G → R in BAC43032 (PubMed:11910074).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei123 – 1231E → EQ in isoform 2. 1 PublicationVSP_055300
Alternative sequencei180 – 1878RPIPHEDL → YGFALLFL in isoform 3. 1 PublicationVSP_055301
Alternative sequencei188 – 333146Missing in isoform 3. 1 PublicationVSP_055302Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002335 Genomic DNA. Translation: AAB64330.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10257.1.
AY072388 mRNA. Translation: AAL62380.1.
AY114697 mRNA. Translation: AAM48016.1.
AK118423 mRNA. Translation: BAC43032.1. Different initiation.
PIRiC84865.
RefSeqiNP_850395.1. NM_180064.2. [Q8VY74-1]
UniGeneiAt.42685.

Genome annotation databases

EnsemblPlantsiAT2G43370.1; AT2G43370.1; AT2G43370. [Q8VY74-1]
GeneIDi818938.
KEGGiath:AT2G43370.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002335 Genomic DNA. Translation: AAB64330.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10257.1.
AY072388 mRNA. Translation: AAL62380.1.
AY114697 mRNA. Translation: AAM48016.1.
AK118423 mRNA. Translation: BAC43032.1. Different initiation.
PIRiC84865.
RefSeqiNP_850395.1. NM_180064.2. [Q8VY74-1]
UniGeneiAt.42685.

3D structure databases

ProteinModelPortaliQ8VY74.
SMRiQ8VY74. Positions 49-152.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4275. 29 interactions.
IntActiQ8VY74. 31 interactions.
STRINGi3702.AT2G43370.1.

Proteomic databases

PaxDbiQ8VY74.
PRIDEiQ8VY74.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G43370.1; AT2G43370.1; AT2G43370. [Q8VY74-1]
GeneIDi818938.
KEGGiath:AT2G43370.

Organism-specific databases

TAIRiAT2G43370.

Phylogenomic databases

eggNOGiKOG0113. Eukaryota.
COG0724. LUCA.
HOGENOMiHOG000006374.
InParanoidiQ8VY74.
KOiK13155.
OMAiCYNAGLY.
PhylomeDBiQ8VY74.

Enzyme and pathway databases

ReactomeiR-ATH-72165. mRNA Splicing - Minor Pathway.

Miscellaneous databases

PROiQ8VY74.

Gene expression databases

GenevisibleiQ8VY74. AT.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Evolutionary conservation of minor U12-type spliceosome between plants and humans."
    Lorkovic Z.J., Lehner R., Forstner C., Barta A.
    RNA 11:1095-1107(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), ALTERNATIVE SPLICING, TISSUE SPECIFICITY, SUBUNIT, PHOSPHORYLATION, INTERACTION WITH SCL28; SCL30; SCL30A; SCL33; SC35; SR30; SR34; RS31; RS40; RSZ21 AND RS2Z33, SUBCELLULAR LOCATION.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 107-333 (ISOFORM 1).
    Strain: cv. Columbia.
  6. "Identification of both shared and distinct proteins in the major and minor spliceosomes."
    Will C.L., Schneider C., Reed R., Luehrmann R.
    Science 284:2003-2005(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, FUNCTION.
  7. "Interactions of Arabidopsis RS domain containing cyclophilins with SR proteins and U1 and U11 small nuclear ribonucleoprotein-specific proteins suggest their involvement in pre-mRNA Splicing."
    Lorkovic Z.J., Lopato S., Pexa M., Lehner R., Barta A.
    J. Biol. Chem. 279:33890-33898(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CYP95.

Entry informationi

Entry nameiU1135_ARATH
AccessioniPrimary (citable) accession number: Q8VY74
Secondary accession number(s): O22851, Q8GX59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: March 1, 2002
Last modified: February 17, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.