##gff-version 3 Q8VY10 UniProtKB Chain 1 907 . . . ID=PRO_0000344361;Note=Probable ubiquitin-conjugating enzyme E2 24 Q8VY10 UniProtKB Domain 662 822 . . . Note=UBC core;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00388 Q8VY10 UniProtKB Region 1 23 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite Q8VY10 UniProtKB Region 485 509 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite Q8VY10 UniProtKB Active site 748 748 . . . Note=Glycyl thioester intermediate;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00388 Q8VY10 UniProtKB Alternative sequence 1 476 . . . ID=VSP_034751;Note=In isoform 2. Missing;Ontology_term=ECO:0000303;evidence=ECO:0000303|Ref.7 Q8VY10 UniProtKB Mutagenesis 748 748 . . . Note=Loss of ubiquitin conjugase activity and loss of down-regulation of PHO1. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22634761;Dbxref=PMID:22634761