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Q8VY10

- UBC24_ARATH

UniProt

Q8VY10 - UBC24_ARATH

Protein

Probable ubiquitin-conjugating enzyme E2 24

Gene

UBC24

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.By similarity

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei748 – 7481Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. cellular response to phosphate starvation Source: TAIR
    2. phosphate ion homeostasis Source: TAIR
    3. phosphate ion transport Source: TAIR
    4. protein ubiquitination Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G33770-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable ubiquitin-conjugating enzyme E2 24 (EC:6.3.2.19)
    Alternative name(s):
    AtPHO2
    Ubiquitin carrier protein 24
    Ubiquitin-protein ligase 24
    Gene namesi
    Name:UBC24
    Synonyms:PHO2
    Ordered Locus Names:At2g33770
    ORF Names:T1B8.8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G33770.

    Pathology & Biotechi

    Disruption phenotypei

    Plants are unable to regulate the amount of phosphate accumulated into shoots.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 907907Probable ubiquitin-conjugating enzyme E2 24PRO_0000344361Add
    BLAST

    Proteomic databases

    PaxDbiQ8VY10.
    PRIDEiQ8VY10.

    Expressioni

    Tissue specificityi

    Expressed in the vascular tissues of cotyledons, leaves, roots, sepals, filaments, anthers and junctions between the inflorescence stems and siliques.2 Publications

    Developmental stagei

    Up-regulated in senescing leaves and maturating seeds.1 Publication

    Inductioni

    Down-regulated by phosphate deprivation.1 Publication

    Gene expression databases

    GenevestigatoriQ8VY10.

    Interactioni

    Protein-protein interaction databases

    BioGridi3290. 1 interaction.
    STRINGi3702.AT2G33770.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VY10.
    SMRiQ8VY10. Positions 630-899.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG315712.
    HOGENOMiHOG000237457.
    InParanoidiQ8VY10.
    KOiK10581.
    OMAiPHEPPMV.
    PhylomeDBiQ8VY10.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8VY10-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEMSLTDSDW DSSSDSGSSE HEEVEFSYGG RAQNIFSNLE ETIGKIDEFL    50
    SFERGFMYGD IVRSATEPSG QSGRVINIDM FVNLESTHGK IMKEVDTKRL 100
    QKLRSISLSD YVINGPWVGR VDKIVERVSV TLDDGTNYEV LVDGQDKLVA 150
    IPPNLLEDSQ YSYYPGQRVQ VKLAHAPRST TWLCGTWRGT QVMGTVCTVE 200
    AGLVYVDWVA SIVMEGDRNL TAPQALQNPE SLTLLPCVSH ASWQLGDWCI 250
    LPGSSHCDIA ERQTPNVAAY NLNECHKTFQ KGFNRNMQNS GLDELFVITK 300
    TKMKVAVMWQ DGSCSLGVDS QQLLPVGAVN AHDFWPEQFV VEKETCNSKK 350
    WGVVKAVNAK EQTVKVQWTI QVEKEATGCV DEVMEEIVSA YELLEHPDFG 400
    FCFSDVVVKL LPEGKFDPNA DTIVATEAKH LLTESDYSGA YFLSSIGVVT 450
    GFKNGSVKVK WANGSTSKVA PCEIWKMERS EYSNSSTVSS EGSVQDLSQK 500
    ISQSDEASSN HQETGLVKLY SVGESCNENI PECSSFFLPK AAIGFITNLA 550
    SSLFGYQGST SVISSHSRCN DSEDQSDSEV LVQETAESYD NSETNSGEVD 600
    MTTTMVNIPI EGKGINKTLD STLLENSRNQ VRFRQFDMVN DCSDHHFLSS 650
    DKGLAQSQVT KSWVKKVQQE WSNLEANLPN TIYVRVCEER MDLLRAALVG 700
    APGTPYHDGL FFFDIMLPPQ YPHEPPMVHY HSGGMRLNPN LYESGRVCLS 750
    LLNTWSGSGT EVWNAGSSSI LQLLLSFQAL VLNEKPYFNE AGYDKQLGRA 800
    EGEKNSVSYN ENAFLITCKS MISMLRKPPK HFEMLVKDHF THRAQHVLAA 850
    CKAYMEGVPV GSSANLQGNS TTNSTGFKIM LSKLYPKLLE AFSEIGVDCV 900
    QEIGPES 907
    Length:907
    Mass (Da):100,484
    Last modified:March 1, 2002 - v1
    Checksum:i6C9CB1D8A3A95359
    GO
    Isoform 2 (identifier: Q8VY10-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-476: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:431
    Mass (Da):47,679
    Checksum:i72A4D61550443B0F
    GO

    Sequence cautioni

    The sequence AAC69130.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 476476Missing in isoform 2. 1 PublicationVSP_034751Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ027037 mRNA. Translation: AAY44863.1.
    U78721 Genomic DNA. Translation: AAC69130.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC08882.1.
    AY074292 mRNA. Translation: AAL66989.1.
    AY091326 mRNA. Translation: AAM14265.1.
    AK229934 mRNA. Translation: BAF01760.1.
    AK230162 mRNA. Translation: BAF01971.1.
    PIRiD84749.
    RefSeqiNP_850218.1. NM_179887.2. [Q8VY10-1]
    UniGeneiAt.14693.

    Genome annotation databases

    EnsemblPlantsiAT2G33770.1; AT2G33770.1; AT2G33770. [Q8VY10-1]
    GeneIDi817943.
    KEGGiath:AT2G33770.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ027037 mRNA. Translation: AAY44863.1 .
    U78721 Genomic DNA. Translation: AAC69130.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC08882.1 .
    AY074292 mRNA. Translation: AAL66989.1 .
    AY091326 mRNA. Translation: AAM14265.1 .
    AK229934 mRNA. Translation: BAF01760.1 .
    AK230162 mRNA. Translation: BAF01971.1 .
    PIRi D84749.
    RefSeqi NP_850218.1. NM_179887.2. [Q8VY10-1 ]
    UniGenei At.14693.

    3D structure databases

    ProteinModelPortali Q8VY10.
    SMRi Q8VY10. Positions 630-899.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 3290. 1 interaction.
    STRINGi 3702.AT2G33770.1-P.

    Proteomic databases

    PaxDbi Q8VY10.
    PRIDEi Q8VY10.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G33770.1 ; AT2G33770.1 ; AT2G33770 . [Q8VY10-1 ]
    GeneIDi 817943.
    KEGGi ath:AT2G33770.

    Organism-specific databases

    TAIRi AT2G33770.

    Phylogenomic databases

    eggNOGi NOG315712.
    HOGENOMi HOG000237457.
    InParanoidi Q8VY10.
    KOi K10581.
    OMAi PHEPPMV.
    PhylomeDBi Q8VY10.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT2G33770-MONOMER.

    Gene expression databases

    Genevestigatori Q8VY10.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, NOMENCLATURE.
    2. "PHO2, microRNA399, and PHR1 define a phosphate-signaling pathway in plants."
      Bari R., Datt Pant B., Stitt M., Scheible W.-R.
      Plant Physiol. 141:988-999(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    3. "pho2, a phosphate overaccumulator, is caused by a nonsense mutation in a microRNA399 target gene."
      Aung K., Lin S.-I., Wu C.-C., Huang Y.-T., Su C.-L., Chiou T.-J.
      Plant Physiol. 141:1000-1011(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE, REGULATION BY MIR399, TISSUE SPECIFICITY.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    7. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    8. "Regulatory network of MicroRNA399 and PHO2 by systemic signaling."
      Lin S.-I., Chiang S.-F., Lin W.-Y., Chen J.-W., Tseng C.-Y., Wu P.-C., Chiou T.-J.
      Plant Physiol. 147:732-746(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, REGULATION BY MIR399.

    Entry informationi

    Entry nameiUBC24_ARATH
    AccessioniPrimary (citable) accession number: Q8VY10
    Secondary accession number(s): P93012, Q0WLN6, Q0WM96
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Systemic regulation by microRNA399 (miR399).

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3