Reviewed, UniProtKB/Swiss-Prot Q8VY10 (UBC24_ARATH)

Names and origin

Protein names

Recommended name:
    Probable ubiquitin-conjugating enzyme E2 24
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase 24
    Ubiquitin carrier protein 24
    AtPHO2

Gene names

Name:	UBC24
Synonyms:	PHO2
Ordered Locus Names:	At2g33770
ORF Names:	T1B8.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	907 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins By similarity. Ref.2 Ref.7
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Tissue specificity	Expressed in the vascular tissues of cotyledons, leaves, roots, sepals, filaments, anthers and junctions between the inflorescence stems and siliques. Ref.2 Ref.3
Developmental stage	Up-regulated in senescing leaves and maturating seeds. Ref.2
Induction	Down-regulated by phosphate deprivation. Ref.2 Ref.7 Ref.3
Disruption phenotype	Plants are unable to regulate the amount of phosphate accumulated into shoots. Ref.3
Miscellaneous	Systemic regulation by microRNA399 (miR399).
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.
Sequence caution	The sequence AAC69130.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular response to phosphate starvation Inferred from mutant phenotype. Source: TAIR modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phosphate ion homeostasis Ref.7 Inferred from mutant phenotype. Source: TAIR phosphate transport Inferred from mutant phenotype. Source: TAIR post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8VY10-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8VY10-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-476: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 907	907	Probable ubiquitin-conjugating enzyme E2 24		PRO_0000344361
Sites
	Active site	748	1	Glycyl thioester intermediate By similarity
Natural variations
	Alternative sequence	1 – 476	476	Missing in isoform 2.		VSP_034751

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 6C9CB1D8A3A95359	FASTA	907	100,484
	10 20 30 40 50 60 MEMSLTDSDW DSSSDSGSSE HEEVEFSYGG RAQNIFSNLE ETIGKIDEFL SFERGFMYGD 70 80 90 100 110 120 IVRSATEPSG QSGRVINIDM FVNLESTHGK IMKEVDTKRL QKLRSISLSD YVINGPWVGR 130 140 150 160 170 180 VDKIVERVSV TLDDGTNYEV LVDGQDKLVA IPPNLLEDSQ YSYYPGQRVQ VKLAHAPRST 190 200 210 220 230 240 TWLCGTWRGT QVMGTVCTVE AGLVYVDWVA SIVMEGDRNL TAPQALQNPE SLTLLPCVSH 250 260 270 280 290 300 ASWQLGDWCI LPGSSHCDIA ERQTPNVAAY NLNECHKTFQ KGFNRNMQNS GLDELFVITK 310 320 330 340 350 360 TKMKVAVMWQ DGSCSLGVDS QQLLPVGAVN AHDFWPEQFV VEKETCNSKK WGVVKAVNAK 370 380 390 400 410 420 EQTVKVQWTI QVEKEATGCV DEVMEEIVSA YELLEHPDFG FCFSDVVVKL LPEGKFDPNA 430 440 450 460 470 480 DTIVATEAKH LLTESDYSGA YFLSSIGVVT GFKNGSVKVK WANGSTSKVA PCEIWKMERS 490 500 510 520 530 540 EYSNSSTVSS EGSVQDLSQK ISQSDEASSN HQETGLVKLY SVGESCNENI PECSSFFLPK 550 560 570 580 590 600 AAIGFITNLA SSLFGYQGST SVISSHSRCN DSEDQSDSEV LVQETAESYD NSETNSGEVD 610 620 630 640 650 660 MTTTMVNIPI EGKGINKTLD STLLENSRNQ VRFRQFDMVN DCSDHHFLSS DKGLAQSQVT 670 680 690 700 710 720 KSWVKKVQQE WSNLEANLPN TIYVRVCEER MDLLRAALVG APGTPYHDGL FFFDIMLPPQ 730 740 750 760 770 780 YPHEPPMVHY HSGGMRLNPN LYESGRVCLS LLNTWSGSGT EVWNAGSSSI LQLLLSFQAL 790 800 810 820 830 840 VLNEKPYFNE AGYDKQLGRA EGEKNSVSYN ENAFLITCKS MISMLRKPPK HFEMLVKDHF 850 860 870 880 890 900 THRAQHVLAA CKAYMEGVPV GSSANLQGNS TTNSTGFKIM LSKLYPKLLE AFSEIGVDCV QEIGPES
	Isoform 2. Checksum: 72A4D61550443B0F	FASTA	431	47,679
	10 20 30 40 50 60 MERSEYSNSS TVSSEGSVQD LSQKISQSDE ASSNHQETGL VKLYSVGESC NENIPECSSF 70 80 90 100 110 120 FLPKAAIGFI TNLASSLFGY QGSTSVISSH SRCNDSEDQS DSEVLVQETA ESYDNSETNS 130 140 150 160 170 180 GEVDMTTTMV NIPIEGKGIN KTLDSTLLEN SRNQVRFRQF DMVNDCSDHH FLSSDKGLAQ 190 200 210 220 230 240 SQVTKSWVKK VQQEWSNLEA NLPNTIYVRV CEERMDLLRA ALVGAPGTPY HDGLFFFDIM 250 260 270 280 290 300 LPPQYPHEPP MVHYHSGGMR LNPNLYESGR VCLSLLNTWS GSGTEVWNAG SSSILQLLLS 310 320 330 340 350 360 FQALVLNEKP YFNEAGYDKQ LGRAEGEKNS VSYNENAFLI TCKSMISMLR KPPKHFEMLV 370 380 390 400 410 420 KDHFTHRAQH VLAACKAYME GVPVGSSANL QGNSTTNSTG FKIMLSKLYP KLLEAFSEIG 430 VDCVQEIGPE S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis." Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J. Plant Physiol. 139:1597-1611(2005) [PubMed: 16339806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, NOMENCLATURE.
[2]	"PHO2, microRNA399, and PHR1 define a phosphate-signaling pathway in plants." Bari R., Datt Pant B., Stitt M., Scheible W.-R. Plant Physiol. 141:988-999(2006) [PubMed: 16679424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
[3]	"pho2, a phosphate overaccumulator, is caused by a nonsense mutation in a microRNA399 target gene." Aung K., Lin S.-I., Wu C.-C., Huang Y.-T., Su C.-L., Chiou T.-J. Plant Physiol. 141:1000-1011(2006) [PubMed: 16679417] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE, REGULATION BY MIR399, TISSUE SPECIFICITY.
[4]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[6]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[7]	"Regulatory network of MicroRNA399 and PHO2 by systemic signaling." Lin S.-I., Chiang S.-F., Lin W.-Y., Chen J.-W., Tseng C.-Y., Wu P.-C., Chiou T.-J. Plant Physiol. 147:732-746(2008) [PubMed: 18390805] [Abstract] Cited for: FUNCTION, REGULATION BY MIR399.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ027037 mRNA. Translation: AAY44863.1. U78721 Genomic DNA. Translation: AAC69130.1. Sequence problems. AY074292 mRNA. Translation: AAL66989.1. AY091326 mRNA. Translation: AAM14265.1. AK229934 mRNA. Translation: BAF01760.1. AK230162 mRNA. Translation: BAF01971.1.
IPI	IPI00518927. IPI00900502.
PIR	D84749.
RefSeq	NP_850218.1.
UniGene	At.14693 Rra.10810 Rsa.3839
3D structure databases
SMR	Q8VY10. Positions 630-861.
ModBase	Search...
Genome annotation databases
GeneID	817943.
GenomeReviews	Gene locus AT2G33770 in contig CT485783_GR.
KEGG	ath:AT2G33770.
NMPDR	fig|3702.1.peg.10468.
Organism-specific databases
TAIR	At2g33770.
Phylogenomic databases
eggNOG	KOG0895.
HOGENOM	HBG319025.
InParanoid	Q8VY10.
OMA	WIGRVIR.
PhylomeDB	Q8VY10.
Gene expression databases
Genevestigator	Q8VY10.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC24_ARATH

Accession

Primary (citable) accession number: Q8VY10
Secondary accession number(s): P93012, Q0WLN6, Q0WM96

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)