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Protein

Probable ubiquitin-conjugating enzyme E2 24

Gene

UBC24

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (By similarity). Mediates PHO1 degradation through multivesicular body-mediated vacuolar proteolysis in response to inorganic phosphate (Pi) availability (PubMed:22634761). Negatively regulates the protein abundance of PHF1 and PHT1s under Pi-sufficient conditions by facilitating the degradation of PHT1 proteins at the endomembrane (PubMed:24122829).By similarity2 Publications

Miscellaneous

MicroRNA399 (miR399) can be sequestered by IPS1, a non-protein coding RNA containing a motif with sequence complementarity to miR399, but with a mismatched loop at the expected miRNA cleavage site. Thus IPS1 mimics the target of miR399 to block the cleavage of UBC24/PHO2 under Pi-deficient conditions.1 Publication

Caution

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.Curated

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei748Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • cellular protein catabolic process Source: TAIR
  • cellular response to phosphate starvation Source: TAIR
  • phosphate ion homeostasis Source: TAIR
  • phosphate ion transport Source: TAIR
  • regulation of phosphate transmembrane transport Source: TAIR

Keywordsi

Molecular functionLigase, Transferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ubiquitin-conjugating enzyme E2 241 Publication (EC:2.3.2.23Curated)
Alternative name(s):
AtPHO21 Publication
E2 ubiquitin-conjugating enzyme 241 Publication
Ubiquitin carrier protein 241 Publication
Ubiquitin-protein ligase 241 Publication
Gene namesi
Name:UBC241 Publication
Synonyms:PHO21 Publication
Ordered Locus Names:At2g33770Imported
ORF Names:T1B8.8Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G33770
TAIRilocus:2057589 AT2G33770

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Plants are unable to regulate the amount of phosphate accumulated into shoots.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi748C → A: Loss of ubiquitin conjugase activity and loss of down-regulation of PHO1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003443611 – 907Probable ubiquitin-conjugating enzyme E2 24Add BLAST907

Proteomic databases

PaxDbiQ8VY10

Expressioni

Tissue specificityi

Expressed in the vascular tissues of cotyledons, leaves, roots, sepals, filaments, anthers and junctions between the inflorescence stems and siliques.2 Publications

Developmental stagei

Up-regulated in senescing leaves and maturating seeds.1 Publication

Inductioni

Down-regulated by phosphate deprivation (PubMed:16679424). Systemically regulated by microRNA399 (miR399) (PubMed:16679424, PubMed:18390805).2 Publications

Gene expression databases

ExpressionAtlasiQ8VY10 baseline and differential
GenevisibleiQ8VY10 AT

Interactioni

Subunit structurei

Interacts with PHO1.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi32901 interactor.
STRINGi3702.AT2G33770.1

Structurei

3D structure databases

ProteinModelPortaliQ8VY10
SMRiQ8VY10
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi480 – 578Ser-richPROSITE-ProRule annotationAdd BLAST99

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0895 Eukaryota
ENOG410XQ7W LUCA
HOGENOMiHOG000237457
InParanoidiQ8VY10
KOiK10581
OMAiGCKNSST
OrthoDBiEOG0936025V
PhylomeDBiQ8VY10

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.101 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VY10-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEMSLTDSDW DSSSDSGSSE HEEVEFSYGG RAQNIFSNLE ETIGKIDEFL
60 70 80 90 100
SFERGFMYGD IVRSATEPSG QSGRVINIDM FVNLESTHGK IMKEVDTKRL
110 120 130 140 150
QKLRSISLSD YVINGPWVGR VDKIVERVSV TLDDGTNYEV LVDGQDKLVA
160 170 180 190 200
IPPNLLEDSQ YSYYPGQRVQ VKLAHAPRST TWLCGTWRGT QVMGTVCTVE
210 220 230 240 250
AGLVYVDWVA SIVMEGDRNL TAPQALQNPE SLTLLPCVSH ASWQLGDWCI
260 270 280 290 300
LPGSSHCDIA ERQTPNVAAY NLNECHKTFQ KGFNRNMQNS GLDELFVITK
310 320 330 340 350
TKMKVAVMWQ DGSCSLGVDS QQLLPVGAVN AHDFWPEQFV VEKETCNSKK
360 370 380 390 400
WGVVKAVNAK EQTVKVQWTI QVEKEATGCV DEVMEEIVSA YELLEHPDFG
410 420 430 440 450
FCFSDVVVKL LPEGKFDPNA DTIVATEAKH LLTESDYSGA YFLSSIGVVT
460 470 480 490 500
GFKNGSVKVK WANGSTSKVA PCEIWKMERS EYSNSSTVSS EGSVQDLSQK
510 520 530 540 550
ISQSDEASSN HQETGLVKLY SVGESCNENI PECSSFFLPK AAIGFITNLA
560 570 580 590 600
SSLFGYQGST SVISSHSRCN DSEDQSDSEV LVQETAESYD NSETNSGEVD
610 620 630 640 650
MTTTMVNIPI EGKGINKTLD STLLENSRNQ VRFRQFDMVN DCSDHHFLSS
660 670 680 690 700
DKGLAQSQVT KSWVKKVQQE WSNLEANLPN TIYVRVCEER MDLLRAALVG
710 720 730 740 750
APGTPYHDGL FFFDIMLPPQ YPHEPPMVHY HSGGMRLNPN LYESGRVCLS
760 770 780 790 800
LLNTWSGSGT EVWNAGSSSI LQLLLSFQAL VLNEKPYFNE AGYDKQLGRA
810 820 830 840 850
EGEKNSVSYN ENAFLITCKS MISMLRKPPK HFEMLVKDHF THRAQHVLAA
860 870 880 890 900
CKAYMEGVPV GSSANLQGNS TTNSTGFKIM LSKLYPKLLE AFSEIGVDCV

QEIGPES
Length:907
Mass (Da):100,484
Last modified:March 1, 2002 - v1
Checksum:i6C9CB1D8A3A95359
GO
Isoform 2 (identifier: Q8VY10-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-476: Missing.

Note: No experimental confirmation available.
Show »
Length:431
Mass (Da):47,679
Checksum:i72A4D61550443B0F
GO

Sequence cautioni

The sequence AAC69130 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0347511 – 476Missing in isoform 2. 1 PublicationAdd BLAST476

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027037 mRNA Translation: AAY44863.1
U78721 Genomic DNA Translation: AAC69130.1 Sequence problems.
CP002685 Genomic DNA Translation: AEC08882.1
AY074292 mRNA Translation: AAL66989.1
AY091326 mRNA Translation: AAM14265.1
AK229934 mRNA Translation: BAF01760.1
AK230162 mRNA Translation: BAF01971.1
PIRiD84749
RefSeqiNP_850218.1, NM_179887.3 [Q8VY10-1]
UniGeneiAt.14693

Genome annotation databases

EnsemblPlantsiAT2G33770.1; AT2G33770.1; AT2G33770 [Q8VY10-1]
GeneIDi817943
GrameneiAT2G33770.1; AT2G33770.1; AT2G33770 [Q8VY10-1]
KEGGiath:AT2G33770

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBC24_ARATH
AccessioniPrimary (citable) accession number: Q8VY10
Secondary accession number(s): P93012, Q0WLN6, Q0WM96
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2002
Last modified: April 25, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome