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Q8VY10

- UBC24_ARATH

UniProt

Q8VY10 - UBC24_ARATH

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Protein

Probable ubiquitin-conjugating enzyme E2 24

Gene

UBC24

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.By similarity

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei748 – 7481Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. cellular protein catabolic process Source: TAIR
  2. cellular response to phosphate starvation Source: TAIR
  3. phosphate ion homeostasis Source: TAIR
  4. phosphate ion transport Source: TAIR
  5. protein ubiquitination Source: UniProtKB-UniPathway
  6. regulation of phosphate transmembrane transport Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G33770-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ubiquitin-conjugating enzyme E2 24 (EC:6.3.2.19)
Alternative name(s):
AtPHO2
Ubiquitin carrier protein 24
Ubiquitin-protein ligase 24
Gene namesi
Name:UBC24
Synonyms:PHO2
Ordered Locus Names:At2g33770
ORF Names:T1B8.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G33770.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: TAIR
  2. Golgi apparatus Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Plants are unable to regulate the amount of phosphate accumulated into shoots.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 907907Probable ubiquitin-conjugating enzyme E2 24PRO_0000344361Add
BLAST

Proteomic databases

PaxDbiQ8VY10.
PRIDEiQ8VY10.

Expressioni

Tissue specificityi

Expressed in the vascular tissues of cotyledons, leaves, roots, sepals, filaments, anthers and junctions between the inflorescence stems and siliques.2 Publications

Developmental stagei

Up-regulated in senescing leaves and maturating seeds.1 Publication

Inductioni

Down-regulated by phosphate deprivation.1 Publication

Gene expression databases

ExpressionAtlasiQ8VY10. baseline and differential.
GenevestigatoriQ8VY10.

Interactioni

Protein-protein interaction databases

BioGridi3290. 1 interaction.
STRINGi3702.AT2G33770.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8VY10.
SMRiQ8VY10. Positions 630-899.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG315712.
HOGENOMiHOG000237457.
InParanoidiQ8VY10.
KOiK10581.
OMAiPHEPPMV.
PhylomeDBiQ8VY10.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8VY10-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEMSLTDSDW DSSSDSGSSE HEEVEFSYGG RAQNIFSNLE ETIGKIDEFL
60 70 80 90 100
SFERGFMYGD IVRSATEPSG QSGRVINIDM FVNLESTHGK IMKEVDTKRL
110 120 130 140 150
QKLRSISLSD YVINGPWVGR VDKIVERVSV TLDDGTNYEV LVDGQDKLVA
160 170 180 190 200
IPPNLLEDSQ YSYYPGQRVQ VKLAHAPRST TWLCGTWRGT QVMGTVCTVE
210 220 230 240 250
AGLVYVDWVA SIVMEGDRNL TAPQALQNPE SLTLLPCVSH ASWQLGDWCI
260 270 280 290 300
LPGSSHCDIA ERQTPNVAAY NLNECHKTFQ KGFNRNMQNS GLDELFVITK
310 320 330 340 350
TKMKVAVMWQ DGSCSLGVDS QQLLPVGAVN AHDFWPEQFV VEKETCNSKK
360 370 380 390 400
WGVVKAVNAK EQTVKVQWTI QVEKEATGCV DEVMEEIVSA YELLEHPDFG
410 420 430 440 450
FCFSDVVVKL LPEGKFDPNA DTIVATEAKH LLTESDYSGA YFLSSIGVVT
460 470 480 490 500
GFKNGSVKVK WANGSTSKVA PCEIWKMERS EYSNSSTVSS EGSVQDLSQK
510 520 530 540 550
ISQSDEASSN HQETGLVKLY SVGESCNENI PECSSFFLPK AAIGFITNLA
560 570 580 590 600
SSLFGYQGST SVISSHSRCN DSEDQSDSEV LVQETAESYD NSETNSGEVD
610 620 630 640 650
MTTTMVNIPI EGKGINKTLD STLLENSRNQ VRFRQFDMVN DCSDHHFLSS
660 670 680 690 700
DKGLAQSQVT KSWVKKVQQE WSNLEANLPN TIYVRVCEER MDLLRAALVG
710 720 730 740 750
APGTPYHDGL FFFDIMLPPQ YPHEPPMVHY HSGGMRLNPN LYESGRVCLS
760 770 780 790 800
LLNTWSGSGT EVWNAGSSSI LQLLLSFQAL VLNEKPYFNE AGYDKQLGRA
810 820 830 840 850
EGEKNSVSYN ENAFLITCKS MISMLRKPPK HFEMLVKDHF THRAQHVLAA
860 870 880 890 900
CKAYMEGVPV GSSANLQGNS TTNSTGFKIM LSKLYPKLLE AFSEIGVDCV

QEIGPES
Length:907
Mass (Da):100,484
Last modified:March 1, 2002 - v1
Checksum:i6C9CB1D8A3A95359
GO
Isoform 2 (identifier: Q8VY10-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-476: Missing.

Note: No experimental confirmation available.

Show »
Length:431
Mass (Da):47,679
Checksum:i72A4D61550443B0F
GO

Sequence cautioni

The sequence AAC69130.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 476476Missing in isoform 2. 1 PublicationVSP_034751Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ027037 mRNA. Translation: AAY44863.1.
U78721 Genomic DNA. Translation: AAC69130.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08882.1.
AY074292 mRNA. Translation: AAL66989.1.
AY091326 mRNA. Translation: AAM14265.1.
AK229934 mRNA. Translation: BAF01760.1.
AK230162 mRNA. Translation: BAF01971.1.
PIRiD84749.
RefSeqiNP_850218.1. NM_179887.2. [Q8VY10-1]
UniGeneiAt.14693.

Genome annotation databases

EnsemblPlantsiAT2G33770.1; AT2G33770.1; AT2G33770. [Q8VY10-1]
GeneIDi817943.
KEGGiath:AT2G33770.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ027037 mRNA. Translation: AAY44863.1 .
U78721 Genomic DNA. Translation: AAC69130.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC08882.1 .
AY074292 mRNA. Translation: AAL66989.1 .
AY091326 mRNA. Translation: AAM14265.1 .
AK229934 mRNA. Translation: BAF01760.1 .
AK230162 mRNA. Translation: BAF01971.1 .
PIRi D84749.
RefSeqi NP_850218.1. NM_179887.2. [Q8VY10-1 ]
UniGenei At.14693.

3D structure databases

ProteinModelPortali Q8VY10.
SMRi Q8VY10. Positions 630-899.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 3290. 1 interaction.
STRINGi 3702.AT2G33770.1-P.

Proteomic databases

PaxDbi Q8VY10.
PRIDEi Q8VY10.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G33770.1 ; AT2G33770.1 ; AT2G33770 . [Q8VY10-1 ]
GeneIDi 817943.
KEGGi ath:AT2G33770.

Organism-specific databases

TAIRi AT2G33770.

Phylogenomic databases

eggNOGi NOG315712.
HOGENOMi HOG000237457.
InParanoidi Q8VY10.
KOi K10581.
OMAi PHEPPMV.
PhylomeDBi Q8VY10.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci ARA:AT2G33770-MONOMER.

Gene expression databases

ExpressionAtlasi Q8VY10. baseline and differential.
Genevestigatori Q8VY10.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, NOMENCLATURE.
  2. "PHO2, microRNA399, and PHR1 define a phosphate-signaling pathway in plants."
    Bari R., Datt Pant B., Stitt M., Scheible W.-R.
    Plant Physiol. 141:988-999(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
  3. "pho2, a phosphate overaccumulator, is caused by a nonsense mutation in a microRNA399 target gene."
    Aung K., Lin S.-I., Wu C.-C., Huang Y.-T., Su C.-L., Chiou T.-J.
    Plant Physiol. 141:1000-1011(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE, REGULATION BY MIR399, TISSUE SPECIFICITY.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  7. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  8. "Regulatory network of MicroRNA399 and PHO2 by systemic signaling."
    Lin S.-I., Chiang S.-F., Lin W.-Y., Chen J.-W., Tseng C.-Y., Wu P.-C., Chiou T.-J.
    Plant Physiol. 147:732-746(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, REGULATION BY MIR399.

Entry informationi

Entry nameiUBC24_ARATH
AccessioniPrimary (citable) accession number: Q8VY10
Secondary accession number(s): P93012, Q0WLN6, Q0WM96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2002
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Systemic regulation by microRNA399 (miR399).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3