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Q8VY10 (UBC24_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ubiquitin-conjugating enzyme E2 24

EC=6.3.2.19
Alternative name(s):
AtPHO2
Ubiquitin carrier protein 24
Ubiquitin-protein ligase 24
Gene names
Name:UBC24
Synonyms:PHO2
Ordered Locus Names:At2g33770
ORF Names:T1B8.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length907 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins By similarity. Ref.2 Ref.8

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Tissue specificity

Expressed in the vascular tissues of cotyledons, leaves, roots, sepals, filaments, anthers and junctions between the inflorescence stems and siliques. Ref.2 Ref.3

Developmental stage

Up-regulated in senescing leaves and maturating seeds. Ref.2

Induction

Down-regulated by phosphate deprivation. Ref.2 Ref.3 Ref.8

Disruption phenotype

Plants are unable to regulate the amount of phosphate accumulated into shoots. Ref.3

Miscellaneous

Systemic regulation by microRNA399 (miR399).

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Sequence caution

The sequence AAC69130.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8VY10-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8VY10-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-476: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 907907Probable ubiquitin-conjugating enzyme E2 24
PRO_0000344361

Sites

Active site7481Glycyl thioester intermediate By similarity

Natural variations

Alternative sequence1 – 476476Missing in isoform 2.
VSP_034751

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 6C9CB1D8A3A95359

FASTA907100,484
        10         20         30         40         50         60 
MEMSLTDSDW DSSSDSGSSE HEEVEFSYGG RAQNIFSNLE ETIGKIDEFL SFERGFMYGD 

        70         80         90        100        110        120 
IVRSATEPSG QSGRVINIDM FVNLESTHGK IMKEVDTKRL QKLRSISLSD YVINGPWVGR 

       130        140        150        160        170        180 
VDKIVERVSV TLDDGTNYEV LVDGQDKLVA IPPNLLEDSQ YSYYPGQRVQ VKLAHAPRST 

       190        200        210        220        230        240 
TWLCGTWRGT QVMGTVCTVE AGLVYVDWVA SIVMEGDRNL TAPQALQNPE SLTLLPCVSH 

       250        260        270        280        290        300 
ASWQLGDWCI LPGSSHCDIA ERQTPNVAAY NLNECHKTFQ KGFNRNMQNS GLDELFVITK 

       310        320        330        340        350        360 
TKMKVAVMWQ DGSCSLGVDS QQLLPVGAVN AHDFWPEQFV VEKETCNSKK WGVVKAVNAK 

       370        380        390        400        410        420 
EQTVKVQWTI QVEKEATGCV DEVMEEIVSA YELLEHPDFG FCFSDVVVKL LPEGKFDPNA 

       430        440        450        460        470        480 
DTIVATEAKH LLTESDYSGA YFLSSIGVVT GFKNGSVKVK WANGSTSKVA PCEIWKMERS 

       490        500        510        520        530        540 
EYSNSSTVSS EGSVQDLSQK ISQSDEASSN HQETGLVKLY SVGESCNENI PECSSFFLPK 

       550        560        570        580        590        600 
AAIGFITNLA SSLFGYQGST SVISSHSRCN DSEDQSDSEV LVQETAESYD NSETNSGEVD 

       610        620        630        640        650        660 
MTTTMVNIPI EGKGINKTLD STLLENSRNQ VRFRQFDMVN DCSDHHFLSS DKGLAQSQVT 

       670        680        690        700        710        720 
KSWVKKVQQE WSNLEANLPN TIYVRVCEER MDLLRAALVG APGTPYHDGL FFFDIMLPPQ 

       730        740        750        760        770        780 
YPHEPPMVHY HSGGMRLNPN LYESGRVCLS LLNTWSGSGT EVWNAGSSSI LQLLLSFQAL 

       790        800        810        820        830        840 
VLNEKPYFNE AGYDKQLGRA EGEKNSVSYN ENAFLITCKS MISMLRKPPK HFEMLVKDHF 

       850        860        870        880        890        900 
THRAQHVLAA CKAYMEGVPV GSSANLQGNS TTNSTGFKIM LSKLYPKLLE AFSEIGVDCV 


QEIGPES 

« Hide

Isoform 2 [UniParc].

Checksum: 72A4D61550443B0F
Show »

FASTA43147,679

References

« Hide 'large scale' references
[1]"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, NOMENCLATURE.
[2]"PHO2, microRNA399, and PHR1 define a phosphate-signaling pathway in plants."
Bari R., Datt Pant B., Stitt M., Scheible W.-R.
Plant Physiol. 141:988-999(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
[3]"pho2, a phosphate overaccumulator, is caused by a nonsense mutation in a microRNA399 target gene."
Aung K., Lin S.-I., Wu C.-C., Huang Y.-T., Su C.-L., Chiou T.-J.
Plant Physiol. 141:1000-1011(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE, REGULATION BY MIR399, TISSUE SPECIFICITY.
[4]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[6]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[7]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[8]"Regulatory network of MicroRNA399 and PHO2 by systemic signaling."
Lin S.-I., Chiang S.-F., Lin W.-Y., Chen J.-W., Tseng C.-Y., Wu P.-C., Chiou T.-J.
Plant Physiol. 147:732-746(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, REGULATION BY MIR399.
+Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ027037 mRNA. Translation: AAY44863.1.
U78721 Genomic DNA. Translation: AAC69130.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08882.1.
AY074292 mRNA. Translation: AAL66989.1.
AY091326 mRNA. Translation: AAM14265.1.
AK229934 mRNA. Translation: BAF01760.1.
AK230162 mRNA. Translation: BAF01971.1.
PIRD84749.
RefSeqNP_850218.1. NM_179887.2.
UniGeneAt.14693.

3D structure databases

ProteinModelPortalQ8VY10.
SMRQ8VY10. Positions 630-899.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid3290. 1 interaction.
STRING3702.AT2G33770.1-P.

Proteomic databases

PaxDbQ8VY10.
PRIDEQ8VY10.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G33770.1; AT2G33770.1; AT2G33770. [Q8VY10-1]
GeneID817943.
KEGGath:AT2G33770.

Organism-specific databases

TAIRAT2G33770.

Phylogenomic databases

eggNOGNOG315712.
HOGENOMHOG000237457.
InParanoidQ8VY10.
KOK10581.
OMADWCILPG.
PhylomeDBQ8VY10.
ProtClustDBCLSN2918162.

Enzyme and pathway databases

BioCycARA:AT2G33770-MONOMER.
UniPathwayUPA00143.

Gene expression databases

GenevestigatorQ8VY10.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBC24_ARATH
AccessionPrimary (citable) accession number: Q8VY10
Secondary accession number(s): P93012, Q0WLN6, Q0WM96
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2002
Last modified: April 16, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names