Clathrin interactor EPSIN 1 - Arabidopsis thaliana (Mouse-ear cress)

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Q8VY07 (EPN1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 73. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Clathrin interactor EPSIN 1

Alternative name(s):
EPSIN-related 1

Gene names

Name:	EPSIN1
Synonyms:	EPSINR1
Ordered Locus Names:	At5g11710
ORF Names:	T22P22.100

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly By similarity. Does not seem to bind to phospholipids. Plays an important role in the vacuolar trafficking of soluble cargo proteins at the trans-Golgi network. Ref.6
Subunit structure	Interacts with clathrin, VTI11, GAMMA-ADR and VSR1. Binds to the deubiquitinating enzyme AMSH3. Ref.6 Ref.7
Subcellular location	Golgi apparatus. Prevacuolar compartment. Cytoplasm. Cytoplasmic vesicle › clathrin-coated vesicle By similarity. Cytoplasm › cytoskeleton. Note: Associated with actin filaments. Ref.6
Tissue specificity	Mostly expressed in cotyledons and flowers, and, to a lower extent, in roots, leaves and siliques (at protein level). Ref.6
Disruption phenotype	Defect in vacuolar trafficking. Ref.6
Sequence similarities	Belongs to the epsin family. Contains 1 ENTH (epsin N-terminal homology) domain.
Sequence caution	The sequence CAB87689.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Cytoplasmic vesicle Cytoskeleton Golgi apparatus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein targeting to vacuole Inferred from mutant phenotype Ref.6. Source: TAIR
Cellular_component	Golgi apparatus Inferred from direct assay Ref.6. Source: TAIR actin filament Inferred from direct assay Ref.6. Source: TAIR clathrin-coated vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell late endosome Inferred from electronic annotation. Source: UniProtKB-SubCell thylakoid Inferred from direct assay PubMed 11826309. Source: TAIR
Molecular_function	clathrin binding Inferred from direct assay Ref.6. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
CHC1	Q0WNJ6	3	EBI-1162785,EBI-1162845
GAMMA-ADR	Q84K16	2	EBI-1162785,EBI-1163115
VSR1	P93026	2	EBI-1162785,EBI-1163008
VTI11	Q9SEL6	3	EBI-1162785,EBI-1162795

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 560

560

Clathrin interactor EPSIN 1

PRO_0000397861

Regions

Domain

20 – 152

133

ENTH

Motif

296 – 300

Clathrin binding By similarity

Motif

320 – 322

ALPHA-ADR binding By similarity

Compositional bias

166 – 261

Ser-rich

Compositional bias

526 – 536

Gln-rich

Experimental info

Sequence conflict

311

N → D in BAF02008. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

Q8VY07 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 29272781B09D2629
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FASTA

560

60,634

        10         20         30         40         50         60 
MDFMKVFDQT VREIKREVNL KVLKVPEMEQ KVLDATDNEP WGPHGTALAE IAQATKKFSE 

        70         80         90        100        110        120 
CQMVMSVLWT RLSETGKDWR YVYKALAVID YLISNGSERA VDEIIEHTYQ ISSLTSFEYV 

       130        140        150        160        170        180 
EPNGKDVGIN VRKKAENIVA LLNNKEKISE IRDKAVANRN KYVGLSSTGI TYKSGSSASF 

       190        200        210        220        230        240 
GGSFQSGSSN FDSYKDRDSR EDKNDYESFQ KSRRGVKTEE QSYTSKKSFS RYGSTDHDNL 

       250        260        270        280        290        300 
SSGKKSPDSA KHRSYVSAAP SNNDDDFDDF DPRGTSSNKP STGSANQVDL FGGDLIGDFL 

       310        320        330        340        350        360 
DSGPTETSST NNNENFQEAD LFADAAFVSA SAQGAEFGSQ TQKQVDLFSA SEPSVTVSSA 

       370        380        390        400        410        420 
PPTVDLFASS ESVVSPEAKI SIPESMATPN IVDPFAAVPM DNFDGSDPFG AFTSHSASVS 

       430        440        450        460        470        480 
TGPQAPSVHG SATNTTSPLS FADSKPQHLQ KKDPFQVKSG IWADSLSRGL IDLNITAPKK 

       490        500        510        520        530        540 
ASLADVGVVG DLSNEDGNKA SAASYYSGWS MGAGSGLGKT GLYSTQQQQQ QQQQQQAPDI 

       550        560 
SDDFFSSLSN QRYQSGGFKQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Sequence analysis of Arabidopsis thaliana E/ANTH-domain-containing proteins: membrane tethers of the clathrin-dependent vesicle budding machinery." Holstein S.E., Oliviusson P. Protoplasma 226:13-21(2005) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[6]	"Arabidopsis EPSIN1 plays an important role in vacuolar trafficking of soluble cargo proteins in plant cells via interactions with clathrin, AP-1, VTI11, and VSR1." Song J., Lee M.H., Lee G.-J., Yoo C.M., Hwang I. Plant Cell 18:2258-2274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH CLATHRIN; VTI11; VSR1 AND GAMMA-ADR.
[7]	"The deubiquitinating enzyme AMSH3 is required for intracellular trafficking and vacuole biogenesis in Arabidopsis thaliana." Isono E., Katsiarimpa A., Mueller I.K., Anzenberger F., Stierhof Y.-D., Geldner N., Chory J., Schwechheimer C. Plant Cell 22:1826-1837(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH AMSH3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL163814 Genomic DNA. Translation: CAB87689.1. Sequence problems. CP002688 Genomic DNA. Translation: AED91713.1. AY074304 mRNA. Translation: AAL67001.1. AY096605 mRNA. Translation: AAM20255.1. AK227905 mRNA. Translation: BAE99875.1. AK230200 mRNA. Translation: BAF02008.1.
IPI	IPI00538379.
PIR	T48530.
RefSeq	NP_196732.2. NM_121209.5.
UniGene	At.32218.
3D structure databases
HSSP	HSSP built from PDB template 1EYH based on UniProtKB O88339.
ProteinModelPortal	Q8VY07.
SMR	Q8VY07. Positions 27-161.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8VY07. 6 interactions.
STRING	3702.AT5G11710.1-P.
Proteomic databases
PaxDb	Q8VY07.
PRIDE	Q8VY07.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G11710.1; AT5G11710.1; AT5G11710.
GeneID	831043.
KEGG	ath:AT5G11710.
Organism-specific databases
TAIR	At5g11710.
Phylogenomic databases
eggNOG	NOG249411.
HOGENOM	HOG000238255.
InParanoid	Q8VY07.
KO	K12471.
OMA	ESLANYN.
PhylomeDB	Q8VY07.
ProtClustDB	CLSN2690151.
Gene expression databases
Genevestigator	Q8VY07.
Family and domain databases
Gene3D	1.25.40.90. 1 hit.
InterPro	IPR008942. ENTH_VHS. IPR013809. Epsin-like_N. IPR001026. Epsin_dom_N. [Graphical view]
Pfam	PF01417. ENTH. 1 hit. [Graphical view]
SMART	SM00273. ENTH. 1 hit. [Graphical view]
SUPFAM	SSF48464. ENTH_VHS. 1 hit.
PROSITE	PS50942. ENTH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EPN1_ARATH

Accession

Primary (citable) accession number: Q8VY07
Secondary accession number(s): Q0WLJ9, Q9LYF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents