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Q8VY06

- PREP2_ARATH

UniProt

Q8VY06 - PREP2_ARATH

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Protein
Presequence protease 2, chloroplastic/mitochondrial
Gene
PREP2, ZNMP2, At1g49630, F14J22.13
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

ATP-independent protease that degrades both mitochondrial and chloroplastic transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference.1 Publication

Cofactori

Binds 1 zinc ion per subunit.
Binds 2 Magnesium ions per subunit By similarity.

Enzyme regulationi

Completely inhibited by the metal chelator orthophenanthroline.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi161 – 1611Zinc By similarity
Active sitei164 – 1641Proton acceptor By similarity
Metal bindingi165 – 1651Zinc By similarity
Metal bindingi261 – 2611Zinc By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metallopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. protein processing Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:GQT-933-MONOMER.
ARA:GQT-934-MONOMER.
ARA:GQT-935-MONOMER.

Protein family/group databases

MEROPSiM16.018.

Names & Taxonomyi

Protein namesi
Recommended name:
Presequence protease 2, chloroplastic/mitochondrial (EC:3.4.24.-)
Short name:
AtPreP2
Short name:
PreP 2
Alternative name(s):
Zinc metalloprotease 2
Short name:
AtZnMP2
Gene namesi
Name:PREP2
Synonyms:ZNMP2
Ordered Locus Names:At1g49630
ORF Names:F14J22.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G49630.

Subcellular locationi

Plastidchloroplast stroma. Mitochondrion matrix 1 Publication

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: UniProtKB-SubCell
  3. mitochondrial matrix Source: UniProtKB-SubCell
  4. mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 8484Chloroplast and mitochondrion Reviewed prediction
Add
BLAST
Chaini85 – 1080996Presequence protease 2, chloroplastic/mitochondrial
PRO_0000249939Add
BLAST

Proteomic databases

PaxDbiQ8VY06.
PRIDEiQ8VY06.

Expressioni

Tissue specificityi

Expressed in leaves, flowers and roots, but not detected in siliques and shoots.1 Publication

Gene expression databases

GenevestigatoriQ8VY06.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

STRINGi3702.AT1G49630.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8VY06.
SMRiQ8VY06. Positions 99-1077.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1026.
HOGENOMiHOG000008829.
InParanoidiQ8VY06.
KOiK06972.
OMAiKGSKAVF.
PhylomeDBiQ8VY06.

Family and domain databases

Gene3Di3.30.830.10. 1 hit.
InterProiIPR011249. Metalloenz_LuxS/M16.
IPR011237. Pept_M16_dom.
IPR011765. Pept_M16_N.
IPR007863. Peptidase_M16_C.
IPR013578. Peptidase_M16C_assoc.
[Graphical view]
PfamiPF08367. M16C_assoc. 1 hit.
PF00675. Peptidase_M16. 1 hit.
PF05193. Peptidase_M16_C. 1 hit.
[Graphical view]
SUPFAMiSSF63411. SSF63411. 4 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VY06-1 [UniParc]FASTAAdd to Basket

« Hide

MLRSLTCSST ITSTSLFFRS FRQLPRSYLS PSSSTTVVGA SGRNIRRLST     50
LEAAGRRLFL RRGLKLLSAA SRGLNGQFSR LSIRAVATQS APSSYPGQDE 100
AEKLGFEKVS EEFISECKSK AVLFKHKKTG CEVMSVSNDD ENKVFGIVFR 150
TPPKDSTGIP HILEHSVLCG SRKYPMKEPF VELLKGSLHT FLNAFTYPDR 200
TCYPVASTNK KDFYNLVDVY LDAVFFPKCV DDVHTFQQEG WHYELNDPSE 250
DISYKGVVFN EMKGVYSQPD NILGRVTQQA LCPENTYGVD SGGDPKDIPK 300
LTFEKFKEFH RQYYHPSNAR IWFYGDDDPV HRLRVLSEYL DMFDASPARD 350
SSKVEPQKLF SRPRRIVEKY PAGEDGDLKK KHMVCLNWLL SDKPLDLQTQ 400
LALGFLDHLM LGTPASPLRK ILLESGLGEA LVNSGMEDEL LQPQFSIGLK 450
GVSDDNVQKV EELVMNTLRK LADEGFDTDA VEASMNTIEF SLRENNTGSS 500
PRGLSLMLQS IAKWIYDMDP FEPLKYEEPL KSLKARIAEK GSKSVFSPLI 550
EEYILNNPHC VTIEMQPDPE KASLEEAEEK SILEKVKASM TEEDLTELAR 600
ATEELRLKQE TPDPPDALKC VPSLNLSDIP KEPIYVPTEV GDINGVKVLR 650
NDLFTNNILY TEVVFDMGSV KHELLQLIPL FCQSLLEMGT QDLTFVQLNQ 700
LIGRKTGGIS VYPLTSSVYG RDDPCSKIIV RGKSMVGRAE DLFNLMNCVL 750
QEVRFTDQQR FKQFVSQSRA RMENRLRGSG QGIAAARMDA MLNVAGWMSE 800
QMGGLSYLEF LHTLEQKVDQ DWEGISSSLE EIRRSFLSRN GCIVNMTADG 850
KSLTNTEKYV GKFLDLLPEN PSGELVTWDA RLPLRNEAIV IPTQVNYVGK 900
AGNIYSSGYK LDGSSYVISK HISNTWLWDR VRVSGGAYGG SCDFDSHSGV 950
FSFLSYRDPN LLKTLDIYDG TGDFLRGLDV DEDTLTKAII GTIGDVDSYQ 1000
LPDAKGYTSL LRHLLNVTDE ERQIRREEIL STSLKDFKEF AEAIDSVSDK 1050
GVAVAVASQE DIDAANRERS NFFEVKKAAL 1080
Length:1,080
Mass (Da):121,131
Last modified:March 1, 2002 - v1
Checksum:i23425D0E7376E39E
GO

Sequence cautioni

The sequence AAG13049.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC011807 Genomic DNA. Translation: AAG13049.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32451.1.
CP002684 Genomic DNA. Translation: AEE32452.1.
CP002684 Genomic DNA. Translation: AEE32453.1.
AY074305 mRNA. Translation: AAL67002.1.
BT004376 mRNA. Translation: AAO42370.1.
PIRiA96533.
RefSeqiNP_175386.2. NM_103851.3.
NP_850961.1. NM_180630.1.
NP_850962.1. NM_180631.1.
UniGeneiAt.27723.

Genome annotation databases

EnsemblPlantsiAT1G49630.1; AT1G49630.1; AT1G49630.
AT1G49630.2; AT1G49630.2; AT1G49630.
AT1G49630.3; AT1G49630.3; AT1G49630.
GeneIDi841387.
KEGGiath:AT1G49630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC011807 Genomic DNA. Translation: AAG13049.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE32451.1 .
CP002684 Genomic DNA. Translation: AEE32452.1 .
CP002684 Genomic DNA. Translation: AEE32453.1 .
AY074305 mRNA. Translation: AAL67002.1 .
BT004376 mRNA. Translation: AAO42370.1 .
PIRi A96533.
RefSeqi NP_175386.2. NM_103851.3.
NP_850961.1. NM_180630.1.
NP_850962.1. NM_180631.1.
UniGenei At.27723.

3D structure databases

ProteinModelPortali Q8VY06.
SMRi Q8VY06. Positions 99-1077.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT1G49630.1-P.

Protein family/group databases

MEROPSi M16.018.

Proteomic databases

PaxDbi Q8VY06.
PRIDEi Q8VY06.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G49630.1 ; AT1G49630.1 ; AT1G49630 .
AT1G49630.2 ; AT1G49630.2 ; AT1G49630 .
AT1G49630.3 ; AT1G49630.3 ; AT1G49630 .
GeneIDi 841387.
KEGGi ath:AT1G49630.

Organism-specific databases

GeneFarmi 2580. 225.
TAIRi AT1G49630.

Phylogenomic databases

eggNOGi COG1026.
HOGENOMi HOG000008829.
InParanoidi Q8VY06.
KOi K06972.
OMAi KGSKAVF.
PhylomeDBi Q8VY06.

Enzyme and pathway databases

BioCyci ARA:GQT-933-MONOMER.
ARA:GQT-934-MONOMER.
ARA:GQT-935-MONOMER.

Gene expression databases

Genevestigatori Q8VY06.

Family and domain databases

Gene3Di 3.30.830.10. 1 hit.
InterProi IPR011249. Metalloenz_LuxS/M16.
IPR011237. Pept_M16_dom.
IPR011765. Pept_M16_N.
IPR007863. Peptidase_M16_C.
IPR013578. Peptidase_M16C_assoc.
[Graphical view ]
Pfami PF08367. M16C_assoc. 1 hit.
PF00675. Peptidase_M16. 1 hit.
PF05193. Peptidase_M16_C. 1 hit.
[Graphical view ]
SUPFAMi SSF63411. SSF63411. 4 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Isolation and identification of a novel mitochondrial metalloprotease (PreP) that degrades targeting presequences in plants."
    Staahl A., Moberg P., Ytterberg J., Panfilov O., Brockenhuus Von Lowenhielm H., Nilsson F., Glaser E.
    J. Biol. Chem. 277:41931-41939(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION.
  5. "Catalysis, subcellular localization, expression and evolution of the targeting peptides degrading protease, AtPreP2."
    Bhushan S., Staahl A., Nilsson S., Lefebvre B., Seki M., Roth C., McWilliam D., Wright S.J., Liberles D.A., Shinozaki K., Bruce B.D., Boutry M., Glaser E.
    Plant Cell Physiol. 46:985-996(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  6. "Two novel targeting peptide degrading proteases, PrePs, in mitochondria and chloroplasts, so similar and still different."
    Staahl A., Nilsson S., Lundberg P., Bhushan S., Biverstaahl H., Moberg P., Morisset M., Vener A., Maeler L., Langel U., Glaser E.
    J. Mol. Biol. 349:847-860(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE SPECIFICITY.

Entry informationi

Entry nameiPREP2_ARATH
AccessioniPrimary (citable) accession number: Q8VY06
Secondary accession number(s): Q9FX91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: March 1, 2002
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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