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Q8VY06

- PREP2_ARATH

UniProt

Q8VY06 - PREP2_ARATH

Protein

Presequence protease 2, chloroplastic/mitochondrial

Gene

PREP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    ATP-independent protease that degrades both mitochondrial and chloroplastic transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference.1 Publication

    Cofactori

    Binds 1 zinc ion per subunit.
    Binds 2 Magnesium ions per subunit.By similarity

    Enzyme regulationi

    Completely inhibited by the metal chelator orthophenanthroline.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi161 – 1611ZincBy similarity
    Active sitei164 – 1641Proton acceptorBy similarity
    Metal bindingi165 – 1651ZincBy similarity
    Metal bindingi261 – 2611ZincBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. metallopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. protein processing Source: TAIR

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:GQT-933-MONOMER.
    ARA:GQT-934-MONOMER.
    ARA:GQT-935-MONOMER.

    Protein family/group databases

    MEROPSiM16.018.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Presequence protease 2, chloroplastic/mitochondrial (EC:3.4.24.-)
    Short name:
    AtPreP2
    Short name:
    PreP 2
    Alternative name(s):
    Zinc metalloprotease 2
    Short name:
    AtZnMP2
    Gene namesi
    Name:PREP2
    Synonyms:ZNMP2
    Ordered Locus Names:At1g49630
    ORF Names:F14J22.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G49630.

    Subcellular locationi

    Plastidchloroplast stroma 1 Publication. Mitochondrion matrix 1 Publication

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: UniProtKB-SubCell
    3. mitochondrial matrix Source: UniProtKB-SubCell
    4. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Mitochondrion, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 8484Chloroplast and mitochondrionSequence AnalysisAdd
    BLAST
    Chaini85 – 1080996Presequence protease 2, chloroplastic/mitochondrialPRO_0000249939Add
    BLAST

    Proteomic databases

    PaxDbiQ8VY06.
    PRIDEiQ8VY06.

    Expressioni

    Tissue specificityi

    Expressed in leaves, flowers and roots, but not detected in siliques and shoots.1 Publication

    Gene expression databases

    GenevestigatoriQ8VY06.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi3702.AT1G49630.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VY06.
    SMRiQ8VY06. Positions 99-1077.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M16 family. PreP subfamily.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1026.
    HOGENOMiHOG000008829.
    InParanoidiQ8VY06.
    KOiK06972.
    OMAiKGSKAVF.
    PhylomeDBiQ8VY06.

    Family and domain databases

    Gene3Di3.30.830.10. 1 hit.
    InterProiIPR011249. Metalloenz_LuxS/M16.
    IPR011237. Pept_M16_dom.
    IPR011765. Pept_M16_N.
    IPR007863. Peptidase_M16_C.
    IPR013578. Peptidase_M16C_assoc.
    [Graphical view]
    PfamiPF08367. M16C_assoc. 1 hit.
    PF00675. Peptidase_M16. 1 hit.
    PF05193. Peptidase_M16_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF63411. SSF63411. 4 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8VY06-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRSLTCSST ITSTSLFFRS FRQLPRSYLS PSSSTTVVGA SGRNIRRLST     50
    LEAAGRRLFL RRGLKLLSAA SRGLNGQFSR LSIRAVATQS APSSYPGQDE 100
    AEKLGFEKVS EEFISECKSK AVLFKHKKTG CEVMSVSNDD ENKVFGIVFR 150
    TPPKDSTGIP HILEHSVLCG SRKYPMKEPF VELLKGSLHT FLNAFTYPDR 200
    TCYPVASTNK KDFYNLVDVY LDAVFFPKCV DDVHTFQQEG WHYELNDPSE 250
    DISYKGVVFN EMKGVYSQPD NILGRVTQQA LCPENTYGVD SGGDPKDIPK 300
    LTFEKFKEFH RQYYHPSNAR IWFYGDDDPV HRLRVLSEYL DMFDASPARD 350
    SSKVEPQKLF SRPRRIVEKY PAGEDGDLKK KHMVCLNWLL SDKPLDLQTQ 400
    LALGFLDHLM LGTPASPLRK ILLESGLGEA LVNSGMEDEL LQPQFSIGLK 450
    GVSDDNVQKV EELVMNTLRK LADEGFDTDA VEASMNTIEF SLRENNTGSS 500
    PRGLSLMLQS IAKWIYDMDP FEPLKYEEPL KSLKARIAEK GSKSVFSPLI 550
    EEYILNNPHC VTIEMQPDPE KASLEEAEEK SILEKVKASM TEEDLTELAR 600
    ATEELRLKQE TPDPPDALKC VPSLNLSDIP KEPIYVPTEV GDINGVKVLR 650
    NDLFTNNILY TEVVFDMGSV KHELLQLIPL FCQSLLEMGT QDLTFVQLNQ 700
    LIGRKTGGIS VYPLTSSVYG RDDPCSKIIV RGKSMVGRAE DLFNLMNCVL 750
    QEVRFTDQQR FKQFVSQSRA RMENRLRGSG QGIAAARMDA MLNVAGWMSE 800
    QMGGLSYLEF LHTLEQKVDQ DWEGISSSLE EIRRSFLSRN GCIVNMTADG 850
    KSLTNTEKYV GKFLDLLPEN PSGELVTWDA RLPLRNEAIV IPTQVNYVGK 900
    AGNIYSSGYK LDGSSYVISK HISNTWLWDR VRVSGGAYGG SCDFDSHSGV 950
    FSFLSYRDPN LLKTLDIYDG TGDFLRGLDV DEDTLTKAII GTIGDVDSYQ 1000
    LPDAKGYTSL LRHLLNVTDE ERQIRREEIL STSLKDFKEF AEAIDSVSDK 1050
    GVAVAVASQE DIDAANRERS NFFEVKKAAL 1080
    Length:1,080
    Mass (Da):121,131
    Last modified:March 1, 2002 - v1
    Checksum:i23425D0E7376E39E
    GO

    Sequence cautioni

    The sequence AAG13049.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC011807 Genomic DNA. Translation: AAG13049.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE32451.1.
    CP002684 Genomic DNA. Translation: AEE32452.1.
    CP002684 Genomic DNA. Translation: AEE32453.1.
    AY074305 mRNA. Translation: AAL67002.1.
    BT004376 mRNA. Translation: AAO42370.1.
    PIRiA96533.
    RefSeqiNP_175386.2. NM_103851.3.
    NP_850961.1. NM_180630.1.
    NP_850962.1. NM_180631.1.
    UniGeneiAt.27723.

    Genome annotation databases

    EnsemblPlantsiAT1G49630.1; AT1G49630.1; AT1G49630.
    AT1G49630.2; AT1G49630.2; AT1G49630.
    AT1G49630.3; AT1G49630.3; AT1G49630.
    GeneIDi841387.
    KEGGiath:AT1G49630.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC011807 Genomic DNA. Translation: AAG13049.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE32451.1 .
    CP002684 Genomic DNA. Translation: AEE32452.1 .
    CP002684 Genomic DNA. Translation: AEE32453.1 .
    AY074305 mRNA. Translation: AAL67002.1 .
    BT004376 mRNA. Translation: AAO42370.1 .
    PIRi A96533.
    RefSeqi NP_175386.2. NM_103851.3.
    NP_850961.1. NM_180630.1.
    NP_850962.1. NM_180631.1.
    UniGenei At.27723.

    3D structure databases

    ProteinModelPortali Q8VY06.
    SMRi Q8VY06. Positions 99-1077.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G49630.1-P.

    Protein family/group databases

    MEROPSi M16.018.

    Proteomic databases

    PaxDbi Q8VY06.
    PRIDEi Q8VY06.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G49630.1 ; AT1G49630.1 ; AT1G49630 .
    AT1G49630.2 ; AT1G49630.2 ; AT1G49630 .
    AT1G49630.3 ; AT1G49630.3 ; AT1G49630 .
    GeneIDi 841387.
    KEGGi ath:AT1G49630.

    Organism-specific databases

    GeneFarmi 2580. 225.
    TAIRi AT1G49630.

    Phylogenomic databases

    eggNOGi COG1026.
    HOGENOMi HOG000008829.
    InParanoidi Q8VY06.
    KOi K06972.
    OMAi KGSKAVF.
    PhylomeDBi Q8VY06.

    Enzyme and pathway databases

    BioCyci ARA:GQT-933-MONOMER.
    ARA:GQT-934-MONOMER.
    ARA:GQT-935-MONOMER.

    Gene expression databases

    Genevestigatori Q8VY06.

    Family and domain databases

    Gene3Di 3.30.830.10. 1 hit.
    InterProi IPR011249. Metalloenz_LuxS/M16.
    IPR011237. Pept_M16_dom.
    IPR011765. Pept_M16_N.
    IPR007863. Peptidase_M16_C.
    IPR013578. Peptidase_M16C_assoc.
    [Graphical view ]
    Pfami PF08367. M16C_assoc. 1 hit.
    PF00675. Peptidase_M16. 1 hit.
    PF05193. Peptidase_M16_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF63411. SSF63411. 4 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Isolation and identification of a novel mitochondrial metalloprotease (PreP) that degrades targeting presequences in plants."
      Staahl A., Moberg P., Ytterberg J., Panfilov O., Brockenhuus Von Lowenhielm H., Nilsson F., Glaser E.
      J. Biol. Chem. 277:41931-41939(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION.
    5. "Catalysis, subcellular localization, expression and evolution of the targeting peptides degrading protease, AtPreP2."
      Bhushan S., Staahl A., Nilsson S., Lefebvre B., Seki M., Roth C., McWilliam D., Wright S.J., Liberles D.A., Shinozaki K., Bruce B.D., Boutry M., Glaser E.
      Plant Cell Physiol. 46:985-996(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    6. "Two novel targeting peptide degrading proteases, PrePs, in mitochondria and chloroplasts, so similar and still different."
      Staahl A., Nilsson S., Lundberg P., Bhushan S., Biverstaahl H., Moberg P., Morisset M., Vener A., Maeler L., Langel U., Glaser E.
      J. Mol. Biol. 349:847-860(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE SPECIFICITY.

    Entry informationi

    Entry nameiPREP2_ARATH
    AccessioniPrimary (citable) accession number: Q8VY06
    Secondary accession number(s): Q9FX91
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2006
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3