Reviewed,
UniProtKB/Swiss-Prot Q8VY06 (PREP2_ARATH)
Last modified
February 9, 2010.
Version 48.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Presequence protease 2, chloroplastic/mitochondrial Short name=PreP 2 Short name=AtPreP2 EC=3.4.24.- Alternative name(s): Zinc metalloprotease 2 Short name=AtZnMP2 | ||||||||
| Gene names |
| ||||||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 1080 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | ATP-independent protease that degrades both mitochondrial and chloroplastic transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. Ref.3 |
| Cofactor | Binds 1 zinc ion per subunit. Binds 2 Magnesium ions per subunit By similarity. |
| Enzyme regulation | Completely inhibited by the metal chelator orthophenanthroline. |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | |
| Tissue specificity | Expressed in leaves, flowers and roots, but not detected in siliques and shoots. Ref.4 |
| Sequence similarities | Belongs to the peptidase M16 family. PreP subfamily. |
| Sequence caution | The sequence AAG13049.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Chloroplast Mitochondrion Plastid |
| Domain | Transit peptide |
| Ligand | Magnesium Metal-binding Zinc |
| Molecular function | Hydrolase Metalloprotease Protease |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | protein maturation by peptide bond cleavage Ref.4 Inferred from direct assay. Source: TAIR proteolysisInferred from electronic annotation. Source: InterPro |
| Cellular component | chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial matrixInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activityInferred from electronic annotation. Source: InterPro zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 84 | 84 | Chloroplast and mitochondrion Potential | ||||||
| Chain | 85 – 1080 | 996 | Presequence protease 2, chloroplastic/mitochondrial | PRO_0000249939 | |||||
Sites | |||||||||
| Active site | 164 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 161 | 1 | Zinc By similarity | ||||||
| Metal binding | 165 | 1 | Zinc By similarity | ||||||
| Metal binding | 261 | 1 | Zinc By similarity | ||||||
Sequences
| ||||||||||||||||||
References
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AC011807 Genomic DNA. Translation: AAG13049.1. Sequence problems. AY074305 mRNA. Translation: AAL67002.1. BT004376 mRNA. Translation: AAO42370.1. |
| IPI | IPI00544499. |
| PIR | A96533. |
| RefSeq | NP_175386.2. NP_850961.1. NP_850962.1. |
| UniGene | At.27723 |
3D structure databases | |
| HSSP | HSSP built from PDB template 2FGE based on UniProtKB Q9LJL3. |
| SMR | Q8VY06. Positions 99-1077. |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | M16.018. |
Proteomic databases | |
| PRIDE | Q8VY06. |
| ProMEX | Q8VY06. |
Genome annotation databases | |
| GeneID | 841387. |
| GenomeReviews | Gene locus AT1G49630 in contig CT485782_GR. |
| KEGG | ath:AT1G49630. |
| NMPDR | fig|3702.1.peg.4435. |
Organism-specific databases | |
| GeneFarm | 2580. 225. |
| TAIR | At1g49630. |
Phylogenomic databases | |
| eggNOG | KOG2019. |
| HOGENOM | HBG413876. |
| InParanoid | Q8VY06. |
| OMA | WLYDGDP. |
| PhylomeDB | Q8VY06. |
Gene expression databases | |
| ArrayExpress | Q8VY06. |
| Genevestigator | Q8VY06. |
| GermOnline | AT1G49630. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR011249. Metalloenz_metal-bd. IPR011237. Pept_M16_core. IPR011765. Pept_M16_N. IPR007863. Peptidase_M16_C. IPR013578. Peptidase_M16C_assoc. [Graphical view] |
| Gene3D | G3DSA:3.30.830.10. Pept_M16_core. 1 hit. |
| Pfam | PF08367. M16C_assoc. 1 hit. PF00675. Peptidase_M16. 1 hit. PF05193. Peptidase_M16_C. 1 hit. [Graphical view] |
| PROSITE | PS00143. INSULINASE. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PREP2_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q8VY06 Secondary accession number(s): Q9FX91 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


