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Protein

Pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1

Gene

ESP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi427 – 434ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • mRNA splicing, via spliceosome Source: GO_Central
  • posttranscriptional gene silencing by RNA Source: TAIR
  • RNA splicing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1Curated (EC:3.6.4.13)
Alternative name(s):
DEAH RNA helicase homolog PRP21 Publication
Protein EMBRYO DEFECTIVE 27331 Publication
Protein ENHANCED SILENCING PHENOTYPE 31 Publication
Gene namesi
Name:ESP31 Publication
Synonyms:EMB27331 Publication
Ordered Locus Names:At1g32490Imported
ORF Names:F5D14.27Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G32490.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • membrane Source: TAIR
  • spliceosomal complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Spliceosome

Pathology & Biotechi

Disruption phenotypei

Embryo defective (PubMed:15266054). Reduced stature, early flowering and altered leaf morphology (PubMed:17008405).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004349401 – 1044Pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1Add BLAST1044

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei135PhosphoserineCombined sources1
Modified residuei138PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ8VY00.

PTM databases

iPTMnetiQ8VY00.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

ExpressionAtlasiQ8VY00. baseline and differential.

Interactioni

Protein-protein interaction databases

IntActiQ8VY00. 1 interactor.
STRINGi3702.AT1G32490.1.

Structurei

3D structure databases

ProteinModelPortaliQ8VY00.
SMRiQ8VY00.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini414 – 577Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST164
Domaini600 – 775Helicase C-terminalPROSITE-ProRule annotationAdd BLAST176

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi524 – 527DEAH boxPROSITE-ProRule annotation4

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0923. Eukaryota.
COG1643. LUCA.
HOGENOMiHOG000175261.
KOiK12813.
OMAiRGKVGCT.
OrthoDBiEOG093601JP.
PhylomeDBiQ8VY00.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.
Isoform 1 (identifier: Q8VY00-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASNDLKTWV SDKLMMLLGY SQAAVVNYLI AMAKKTKSPT ELVGELVDYG
60 70 80 90 100
FSSSGDTRSF AEEIFARVPR KTAGVNLYQK HEAEAAMLVR KQKTYALLDA
110 120 130 140 150
DDDEDEVVVE KKSSVSESRK SDKGKKRFRK KSGQSDESDG EVAVREDSRH
160 170 180 190 200
VRRKVSEEDD GSESEEERVR DQKEREELEQ HLKDRDTART RKLTEQTLSK
210 220 230 240 250
KEKEEAVRRA NALEKDDLYS LRKVSRQEYL KKREQKKLDE LRDEIEDEQY
260 270 280 290 300
LFGGEKLTET ELREFRYKKE LYDLVKKRTQ DEDNVEEYRI PDAYDQEGGV
310 320 330 340 350
DQEKRFSVAV QRYRDLDSTE KMNPFAEQEA WEDHQIGKAT LKFGAKNKQA
360 370 380 390 400
SDDYQFVFED QINFIKESVM AGENYEDAMD AKQKSQDLAE KTALEELQEV
410 420 430 440 450
RRSLPIYTYR DQLLKAVEEH QVLVIVGDTG SGKTTQIPQY LHEAGYTKRG
460 470 480 490 500
KVGCTQPRRV AAMSVAARVA QEMGVKLGHE VGYSIRFEDC TSDKTVLKYM
510 520 530 540 550
TDGMLLRELL GEPDLASYSV VIVDEAHERT LSTDILFGLV KDIARFRPDL
560 570 580 590 600
KLLISSATMD AEKFSDYFDT APIFSFPGRR YPVEINYTSA PEADYMDAAI
610 620 630 640 650
VTILTIHVRE PLGDILVFFT GQEEIETAEE ILKHRIRGLG TKIRELIICP
660 670 680 690 700
IYANLPSELQ AKIFEPTPEG ARKVVLATNI AETSLTIDGI KYVVDPGFSK
710 720 730 740 750
MKSYNPRTGM ESLLITPISK ASATQRAGRA GRTSPGKCYR LYTAFNYNND
760 770 780 790 800
LEENTVPEVQ RTNLASVVLA LKSLGIHDLI NFDFMDPPPA EALVKSLELL
810 820 830 840 850
FALGALNKLG ELTKAGRRMA EFPLDPMLSK MIVVSDKYKC SDEIISIAAM
860 870 880 890 900
LSIGGSIFYR PKDKQVHADN ARMNFHTGNV GDHIALLKVY SSWKETNFST
910 920 930 940 950
QWCYENYIQV RSMKRARDIR DQLEGLLERV EIDISSNLNE LDSVRKSIVA
960 970 980 990 1000
GFFPHTAKLQ KNGSYRTVKH PQTVHIHPNS GLSQVLPRWV VYHELVLTSK
1010 1020 1030 1040
EYMRQVTELK PEWLIELAPH YYQLKDVEDA ASKKMPKGAG KAAM
Length:1,044
Mass (Da):118,832
Last modified:March 1, 2002 - v1
Checksum:i187B02E796AF0E18
GO

Sequence cautioni

The sequence AAF81347 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007767 Genomic DNA. Translation: AAF81347.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE31492.1.
AY074318 mRNA. Translation: AAL67014.1.
AY133872 mRNA. Translation: AAM91806.1.
PIRiC86450.
RefSeqiNP_174527.2. NM_102984.5. [Q8VY00-1]
UniGeneiAt.40132.

Genome annotation databases

EnsemblPlantsiAT1G32490.1; AT1G32490.1; AT1G32490. [Q8VY00-1]
GeneIDi840143.
GrameneiAT1G32490.1; AT1G32490.1; AT1G32490.
KEGGiath:AT1G32490.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Seed defective Arabidopsis mutants
Splicing Related Gene Database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007767 Genomic DNA. Translation: AAF81347.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE31492.1.
AY074318 mRNA. Translation: AAL67014.1.
AY133872 mRNA. Translation: AAM91806.1.
PIRiC86450.
RefSeqiNP_174527.2. NM_102984.5. [Q8VY00-1]
UniGeneiAt.40132.

3D structure databases

ProteinModelPortaliQ8VY00.
SMRiQ8VY00.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VY00. 1 interactor.
STRINGi3702.AT1G32490.1.

PTM databases

iPTMnetiQ8VY00.

Proteomic databases

PRIDEiQ8VY00.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G32490.1; AT1G32490.1; AT1G32490. [Q8VY00-1]
GeneIDi840143.
GrameneiAT1G32490.1; AT1G32490.1; AT1G32490.
KEGGiath:AT1G32490.

Organism-specific databases

TAIRiAT1G32490.

Phylogenomic databases

eggNOGiKOG0923. Eukaryota.
COG1643. LUCA.
HOGENOMiHOG000175261.
KOiK12813.
OMAiRGKVGCT.
OrthoDBiEOG093601JP.
PhylomeDBiQ8VY00.

Enzyme and pathway databases

ReactomeiR-ATH-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiQ8VY00.

Gene expression databases

ExpressionAtlasiQ8VY00. baseline and differential.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiESP3_ARATH
AccessioniPrimary (citable) accession number: Q8VY00
Secondary accession number(s): Q9LQK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 9, 2015
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.