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Protein

Alpha-galactosidase 3

Gene

AGAL3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May regulate leaf (and possibly other organ) development by functioning in cell wall loosening and cell wall expansion.By similarity

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei191SubstrateBy similarity1
Active sitei193NucleophileBy similarity1
Binding sitei244SubstrateBy similarity1
Active sitei248Proton donorBy similarity1
Binding sitei248SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT3G56310-MONOMER.
ReactomeiR-ATH-1660662. Glycosphingolipid metabolism.
R-ATH-6798695. Neutrophil degranulation.

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase 31 Publication (EC:3.2.1.22By similarity)
Short name:
AtAGAL31 Publication
Alternative name(s):
Alpha-D-galactoside galactohydrolase 3Curated
MelibiaseCurated
Gene namesi
Name:AGAL31 Publication
Ordered Locus Names:At3g56310Imported
ORF Names:F18O21.270Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G56310.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: UniProtKB-SubCell
  • plant-type cell wall Source: GO_Central
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000043184731 – 437Alpha-galactosidase 3Sequence analysisAdd BLAST407

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi85 ↔ 117By similarity
Glycosylationi88N-linked (GlcNAc...)PROSITE-ProRule annotation1
Disulfide bondi165 ↔ 195By similarity
Glycosylationi214N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi250N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi315N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi408N-linked (GlcNAc...)PROSITE-ProRule annotation1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8VXZ7.
PRIDEiQ8VXZ7.

Expressioni

Inductioni

May be processed or degraded in a VPEgamma-dependent manner in vacuoles.1 Publication

Gene expression databases

ExpressionAtlasiQ8VXZ7. baseline and differential.
GenevisibleiQ8VXZ7. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G56310.1.

Structurei

3D structure databases

ProteinModelPortaliQ8VXZ7.
SMRiQ8VXZ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni115 – 116Substrate bindingBy similarity2
Regioni226 – 230Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2366. Eukaryota.
ENOG410XPF1. LUCA.
HOGENOMiHOG000161224.
KOiK07407.
OMAiDSWASMT.
OrthoDBiEOG09360A7S.
PhylomeDBiQ8VXZ7.

Family and domain databases

CDDicd14792. GH27. 1 hit.
Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_27/36_CS.
IPR013780. Glyco_hydro_b.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF16499. Melibiase_2. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VXZ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVIMKKMKDS VLFLVVGLFS LSVLVSQSIA GRVKAPLLQS NTGGLVFSKS
60 70 80 90 100
FNSIYDTSMY GRLQLNNGLA RTPQMGWNSW NFFACNINET VIKETADALV
110 120 130 140 150
SSGLADLGYI HVNIDDCWSN LLRDSEGQLV PHPETFPSGI KLLADYVHSK
160 170 180 190 200
GLKLGIYSDA GVFTCEVHPG SLFHEVDDAD IFASWGVDYL KYDNCFNLGI
210 220 230 240 250
KPIERYPPMR DALNATGRSI FYSLCEWGVD DPALWAKEVG NSWRTTDDIN
260 270 280 290 300
DTWASMTTIA DLNNKWAAYA GPGGWNDPDM LEIGNGGMTY EEYRGHFSIW
310 320 330 340 350
ALMKAPLLIG CDVRNMTAET LEILSNKEII AVNQDPLGVQ GRKIQANGEN
360 370 380 390 400
DCQQVWSGPL SGDRMVVALW NRCSEPATIT ASWDMIGLES TISVSVRDLW
410 420 430
QHKDVTENTS GSFEAQVDAH DCHMYVLTPQ TVSHSDV
Length:437
Mass (Da):48,363
Last modified:March 1, 2002 - v1
Checksum:i5DC7C91065C8B033
GO
Isoform 2 (identifier: Q8VXZ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     115-138: Missing.

Note: Derived from EST data. No experimental confirmation available.Imported
Show »
Length:413
Mass (Da):45,638
Checksum:iE07A9422FACEFFA7
GO

Sequence cautioni

The sequence CAB87430 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_057450115 – 138Missing in isoform 2. Add BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL163763 Genomic DNA. Translation: CAB87430.1. Different initiation.
CP002686 Genomic DNA. Translation: AEE79507.1.
CP002686 Genomic DNA. Translation: AEE79508.1.
AY074321 mRNA. Translation: AAL67017.1.
AY114020 mRNA. Translation: AAM45068.1.
PIRiT47748.
RefSeqiNP_191190.2. NM_115489.6. [Q8VXZ7-1]
NP_974447.1. NM_202718.2. [Q8VXZ7-2]
UniGeneiAt.23883.
At.71191.

Genome annotation databases

EnsemblPlantsiAT3G56310.1; AT3G56310.1; AT3G56310. [Q8VXZ7-1]
AT3G56310.2; AT3G56310.2; AT3G56310. [Q8VXZ7-2]
GeneIDi824798.
GrameneiAT3G56310.1; AT3G56310.1; AT3G56310.
AT3G56310.2; AT3G56310.2; AT3G56310.
KEGGiath:AT3G56310.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL163763 Genomic DNA. Translation: CAB87430.1. Different initiation.
CP002686 Genomic DNA. Translation: AEE79507.1.
CP002686 Genomic DNA. Translation: AEE79508.1.
AY074321 mRNA. Translation: AAL67017.1.
AY114020 mRNA. Translation: AAM45068.1.
PIRiT47748.
RefSeqiNP_191190.2. NM_115489.6. [Q8VXZ7-1]
NP_974447.1. NM_202718.2. [Q8VXZ7-2]
UniGeneiAt.23883.
At.71191.

3D structure databases

ProteinModelPortaliQ8VXZ7.
SMRiQ8VXZ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G56310.1.

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

Proteomic databases

PaxDbiQ8VXZ7.
PRIDEiQ8VXZ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G56310.1; AT3G56310.1; AT3G56310. [Q8VXZ7-1]
AT3G56310.2; AT3G56310.2; AT3G56310. [Q8VXZ7-2]
GeneIDi824798.
GrameneiAT3G56310.1; AT3G56310.1; AT3G56310.
AT3G56310.2; AT3G56310.2; AT3G56310.
KEGGiath:AT3G56310.

Organism-specific databases

TAIRiAT3G56310.

Phylogenomic databases

eggNOGiKOG2366. Eukaryota.
ENOG410XPF1. LUCA.
HOGENOMiHOG000161224.
KOiK07407.
OMAiDSWASMT.
OrthoDBiEOG09360A7S.
PhylomeDBiQ8VXZ7.

Enzyme and pathway databases

BioCyciARA:AT3G56310-MONOMER.
ReactomeiR-ATH-1660662. Glycosphingolipid metabolism.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ8VXZ7.

Gene expression databases

ExpressionAtlasiQ8VXZ7. baseline and differential.
GenevisibleiQ8VXZ7. AT.

Family and domain databases

CDDicd14792. GH27. 1 hit.
Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_27/36_CS.
IPR013780. Glyco_hydro_b.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF16499. Melibiase_2. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGAL3_ARATH
AccessioniPrimary (citable) accession number: Q8VXZ7
Secondary accession number(s): F4IZE4, Q9LYL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 4, 2015
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.