Reviewed, UniProtKB/Swiss-Prot Q8VXQ9 (G3PA_SCEVA)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic
    EC=1.2.1.13
Alternative name(s):
    NADP-dependent glyceraldehydephosphate dehydrogenase A
      Short name=GAPDHA

Gene names

Name:

GapA

Organism

Scenedesmus vacuolatus (Chlorella fusca var. vacuolata)

Taxonomic identifier

77546 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Sphaeropleales › Scenedesmaceae › Scenedesmus

Protein attributes

Sequence length	314 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Homotetramer By similarity.
Subcellular location	Plastid › chloroplast By similarity.
Induction	Repressed by addition of D-galactose, D-mannose, D-glucose, D-ribose or sucrose, but not by the addition of non-metabolizable sugar analogs. Ref.1
Miscellaneous	GAPDHB, the second subunit found in plants, is absent in algae. Algae contain three forms of GAPDH: two cytosolic forms which participate in glycolysis and one chloroplastic form which participates in photosynthesis. These three forms are encoded by distinct genes.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›314	›314	Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic		PRO_0000292345
Regions
	Nucleotide binding	5 – 6	2	NADP By similarity
	Region	147 – 149	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	206 – 207	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	148	1	Nucleophile By similarity
	Binding site	29	1	NADP By similarity
	Binding site	74	1	NADP; via carbonyl oxygen By similarity
	Binding site	178	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	193	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	229	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	311	1	NADP By similarity
	Site	175	1	Activates thiol group during catalysis By similarity
Amino acid modifications
	Disulfide bond	13 ↔ 283		By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	314	1

Sequences

	Sequence		Length	Mass (Da)
	Q8VXQ9-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: F0F3CA15BDC4DB77	FASTA	314	33,574
	10 20 30 40 50 60 NGFGRIGRNF LRCLETRQNS LLEVIAINDS GGVKQASHLL KYDSTLGKFD ADVKIVDDGH 70 80 90 100 110 120 ISVNGKSIRV VSSRDPTKLP WGEMEIDLVI EGTGVFIDTP GASKHIEAGA KKVLITAPAK 130 140 150 160 170 180 GSDIPTYVVG VNAHDYKHSD AIISNASCTT NCLAPFVKVL DEKFGIVKGT MTNTHSYTGD 190 200 210 220 230 240 QRLLDASHRD LRRARAAALN IVPTTTGAAK AVALVLPKLK GKLNGIALRV PTPNVSVVDL 250 260 270 280 290 300 VVQVEKKTFA EEINNAFKEA AAGSLNGVLA VSDEPLVSVD FRCTDVSSTI DSSLTMVMGA 310 DMVKVVAWYD NEWG

References

[1]

"Sugar-mediated transcriptional regulation of the Gap gene system and concerted photosystem II functional modulation in the microalga Scenedesmus vacuolatus." Valverde F., Ortega J.M., Losada M., Serrano A. Planta 221:937-952(2005) [PubMed: 15830207] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION. Strain: SAG 211-8b.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ252208 mRNA. Translation: CAC81011.1.
3D structure databases
HSSP	HSSP built from PDB template 1RM4 based on UniProtKB P19866.
SMR	Q8VXQ9. Positions 1-314.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.2.1.13. 294419.
Family and domain databases
InterPro	IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020832. GlycerAld_3-P_DH_cat_sub. IPR020831. GlycerAld_3-P_DH_family. IPR020828. GlycerAld_3-P_DH_NAD(P)_bd. IPR000173. GlycerAld_3-P_DH_subfam. IPR006424. Glyceraldehyde-3-P_DH_1. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PA_SCEVA

Accession

Primary (citable) accession number: Q8VXQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)