Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aldehyde dehydrogenase

Gene

ALDH

Organism
Craterostigma plantagineum (Blue gem) (Torenia plantagineum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Oxidizes nonanal, propionaldehyde and acetaldehyde in vitro, in the following decreasing order of reactivity: nonanal, propionaldehyde, acetaldehyde.1 Publication

Catalytic activityi

An aldehyde + NAD+ + H2O = a carboxylate + NADH.

Kineticsi

Measured at pH 9.5 for all experiments.

  1. KM=2.2 µM for nonanal1 Publication
  2. KM=267 µM for propionaldehyde1 Publication
  3. KM=32.3 mM for acetaldehyde1 Publication

Vmax=0.03 µmol/sec/mg enzyme with nonanal as substrate1 Publication

Vmax=0.196 µmol/sec/mg enzyme with propionaldehyde as substrate1 Publication

Vmax=0.102 µmol/sec/mg enzyme with acetaldehyde as substrate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei117 – 1171Transition state stabilizerBy similarity
Active sitei212 – 2121Proton acceptorBy similarity
Active sitei247 – 2471NucleophileBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi190 – 1956NADBy similarity

GO - Molecular functioni

  1. aldehyde dehydrogenase (NAD) activity Source: UniProtKB-EC
  2. aldehyde dehydrogenase [NAD(P)+] activity Source: InterPro

GO - Biological processi

  1. cellular aldehyde metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Stress response

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Aldehyde dehydrogenase (EC:1.2.1.3)
Alternative name(s):
Cp-ALDH
Gene namesi
Name:ALDH
OrganismiCraterostigma plantagineum (Blue gem) (Torenia plantagineum)
Taxonomic identifieri4153 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLinderniaceaeCraterostigma

Subcellular locationi

Plastidamyloplast 1 Publication. Plastidchloroplast 1 Publication

GO - Cellular componenti

  1. amyloplast Source: UniProtKB-SubCell
  2. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Amyloplast, Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479Aldehyde dehydrogenasePRO_0000256068Add
BLAST

Expressioni

Inductioni

By abscisic acid (ABA) and dehydration.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8VXQ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR012394. Aldehyde_DH_NAD(P).
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036492. ALDH. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8VXQ2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQVDAEGVV DGLRRTYISG KTKSYEWRVS QLKALLKITT HHDKEVVEAL
60 70 80 90 100
RADLKKPEHE AYVHEIFMVS NACKSALKEL HQWMKPQKVK TSLATYPSSA
110 120 130 140 150
EIVSEPLGVV LVITAWNYPF LLALDPMIGA IAAGNCVVLK PSEIAPATSA
160 170 180 190 200
LLAKLLNQYV DTSAIRVVEG AVPEMQALLD QRWDKIFYTG SSKVGQIVLS
210 220 230 240 250
SAAKHLTPVV LELGGKCPTV VDANIDLKVA ARRIISWKWS GNSGQTCISP
260 270 280 290 300
DYIITTEENA PKLVDAIKCE LESFYGKDPL KSQDMSSIIN ERQFERMTGL
310 320 330 340 350
LDDKKVSDKI VYGGQSDKSN LKIAPTILLD VSEDSSVMSE EIFGPLLPII
360 370 380 390 400
TVGKIEECYK IIASKPKPLA AYLFTNDKKR TEEFVSNVSA GGITINDIAL
410 420 430 440 450
HFLEPRLPFG GVGESGMGSY HGKFSFDAFS HKKSVLKRSF GGEVAARYPP
460 470
YAPWKLHFME AILQGDIFGL LKAWLGWSS
Length:479
Mass (Da):52,741
Last modified:March 1, 2002 - v1
Checksum:i47F266AE6CF77FCE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ306960 mRNA. Translation: CAC84900.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ306960 mRNA. Translation: CAC84900.1.

3D structure databases

ProteinModelPortaliQ8VXQ2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR012394. Aldehyde_DH_NAD(P).
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036492. ALDH. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Novel ABA- and dehydration-inducible aldehyde dehydrogenase genes isolated from the resurrection plant Craterostigma plantagineum and Arabidopsis thaliana."
    Kirch H.-H., Nair A., Bartels D.
    Plant J. 28:555-567(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, INDUCTION.

Entry informationi

Entry nameiALDH_CRAPL
AccessioniPrimary (citable) accession number: Q8VXQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: March 1, 2002
Last modified: January 7, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.