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Protein

Protein disulfide isomerase-like 1-3

Gene

PDIL1-3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds.By similarity

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei128NucleophileBy similarity1
Active sitei131NucleophileBy similarity1
Sitei190Lowers pKa of C-terminal Cys of first active siteBy similarity1
Active sitei467NucleophileBy similarity1
Sitei468Contributes to redox potential valueBy similarity1
Sitei469Contributes to redox potential valueBy similarity1
Active sitei470NucleophileBy similarity1
Sitei532Lowers pKa of C-terminal Cys of second active siteBy similarity1

GO - Molecular functioni

  • protein disulfide isomerase activity Source: TAIR

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • protein folding Source: GO_Central
  • response to endoplasmic reticulum stress Source: TAIR

Keywordsi

Molecular functionIsomerase

Enzyme and pathway databases

ReactomeiR-ATH-3299685 Detoxification of Reactive Oxygen Species

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide isomerase-like 1-3 (EC:5.3.4.1)
Short name:
AtPDIL1-3
Alternative name(s):
Protein disulfide isomerase 1
Short name:
AtPDI1
Protein disulfide isomerase-like 2-1
Short name:
AtPDIL2-1
Gene namesi
Name:PDIL1-3
Synonyms:PDI1, PDI72, PDIL2-1
Ordered Locus Names:At3g54960
ORF Names:F28P10.60, T15C9.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G54960
TAIRilocus:2082712 AT3G54960

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_500006591726 – 579Protein disulfide isomerase-like 1-3Add BLAST554

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi27N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi108N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi115N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi128 ↔ 131Redox-activePROSITE-ProRule annotation
Glycosylationi209N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi293N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi313N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi338N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi467 ↔ 470Redox-activePROSITE-ProRule annotation
Glycosylationi520N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8VX13
PRIDEiQ8VX13

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Inductioni

By chemically-induced ER stress response.1 Publication

Gene expression databases

ExpressionAtlasiQ8VX13 baseline and differential
GenevisibleiQ8VX13 AT

Interactioni

Protein-protein interaction databases

BioGridi9977, 1 interactor
IntActiQ8VX13, 1 interactor
STRINGi3702.AT3G54960.1

Structurei

3D structure databases

ProteinModelPortaliQ8VX13
SMRiQ8VX13
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 204Thioredoxin 1PROSITE-ProRule annotationAdd BLAST124
Domaini416 – 546Thioredoxin 2PROSITE-ProRule annotationAdd BLAST131

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi576 – 579Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0190 Eukaryota
COG0526 LUCA
HOGENOMiHOG000162459
InParanoidiQ8VX13
KOiK09580
OMAiGNEDMRK
OrthoDBiEOG093607A9
PhylomeDBiQ8VX13

Family and domain databases

InterProiView protein in InterPro
IPR005792 Prot_disulphide_isomerase
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF00085 Thioredoxin, 2 hits
SUPFAMiSSF52833 SSF52833, 4 hits
TIGRFAMsiTIGR01130 ER_PDI_fam, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00194 THIOREDOXIN_1, 2 hits
PS51352 THIOREDOXIN_2, 2 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VX13-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSSTSISL LLFVSFILLL VNSRAENASS GSDLDEELAF LAAEESKEQS
60 70 80 90 100
HGGGSYHEEE HDHQHRDFEN YDDLEQGGGE FHHGDHGYEE EPLPPVDEKD
110 120 130 140 150
VAVLTKDNFT EFVGNNSFAM VEFYAPWCGA CQALTPEYAA AATELKGLAA
160 170 180 190 200
LAKIDATEEG DLAQKYEIQG FPTVFLFVDG EMRKTYEGER TKDGIVTWLK
210 220 230 240 250
KKASPSIHNI TTKEEAERVL SAEPKLVFGF LNSLVGSESE ELAAASRLED
260 270 280 290 300
DLSFYQTASP DIAKLFEIET QVKRPALVLL KKEEEKLARF DGNFTKTAIA
310 320 330 340 350
EFVSANKVPL VINFTREGAS LIFESSVKNQ LILFAKANES EKHLPTLREV
360 370 380 390 400
AKSFKGKFVF VYVQMDNEDY GEAVSGFFGV TGAAPKVLVY TGNEDMRKFI
410 420 430 440 450
LDGELTVNNI KTLAEDFLAD KLKPFYKSDP LPENNDGDVK VIVGNNFDEI
460 470 480 490 500
VLDESKDVLL EIYAPWCGHC QSFEPIYNKL GKYLKGIDSL VVAKMDGTSN
510 520 530 540 550
EHPRAKADGF PTILFFPGGN KSFDPIAVDV DRTVVELYKF LKKHASIPFK
560 570
LEKPATPEPV ISTMKSDEKI EGDSSKDEL
Length:579
Mass (Da):64,212
Last modified:March 1, 2002 - v1
Checksum:i0DF50E500B24B829
GO
Isoform 2 (identifier: Q8VX13-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     507-518: ADGFPTILFFPG → VIKKKELRKSFW
     519-579: Missing.

Note: No experimental confirmation available.
Show »
Length:518
Mass (Da):57,691
Checksum:iB3AA1BB3BAFE49FF
GO

Sequence cautioni

The sequence CAB41088 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_039979507 – 518ADGFP…LFFPG → VIKKKELRKSFW in isoform 2. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_039980519 – 579Missing in isoform 2. 1 PublicationAdd BLAST61

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK318844 mRNA Translation: BAH56959.1
AJ271376 mRNA Translation: CAC81067.1
AL049655 Genomic DNA Translation: CAB41088.1 Sequence problems.
AL132970 Genomic DNA No translation available.
CP002686 Genomic DNA Translation: AEE79318.1
CP002686 Genomic DNA Translation: AEE79319.1
AY093115 mRNA Translation: AAM13114.1
BT000172 mRNA Translation: AAN15491.1
PIRiT06724
RefSeqiNP_001118842.1, NM_001125370.1 [Q8VX13-2]
NP_191056.2, NM_115353.5 [Q8VX13-1]
UniGeneiAt.22358

Genome annotation databases

EnsemblPlantsiAT3G54960.1; AT3G54960.1; AT3G54960 [Q8VX13-1]
AT3G54960.2; AT3G54960.2; AT3G54960 [Q8VX13-2]
GeneIDi824661
GrameneiAT3G54960.1; AT3G54960.1; AT3G54960 [Q8VX13-1]
AT3G54960.2; AT3G54960.2; AT3G54960 [Q8VX13-2]
KEGGiath:AT3G54960

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPDI13_ARATH
AccessioniPrimary (citable) accession number: Q8VX13
Secondary accession number(s): B3H5N9, Q9SV44
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: March 1, 2002
Last modified: May 23, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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