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Protein

Protein disulfide isomerase-like 1-3

Gene

PDIL1-3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds.By similarity

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei128NucleophileBy similarity1
Active sitei131NucleophileBy similarity1
Sitei190Lowers pKa of C-terminal Cys of first active siteBy similarity1
Active sitei467NucleophileBy similarity1
Sitei468Contributes to redox potential valueBy similarity1
Sitei469Contributes to redox potential valueBy similarity1
Active sitei470NucleophileBy similarity1
Sitei532Lowers pKa of C-terminal Cys of second active siteBy similarity1

GO - Molecular functioni

  • protein disulfide isomerase activity Source: TAIR

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • protein folding Source: GO_Central
  • response to endoplasmic reticulum stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide isomerase-like 1-3 (EC:5.3.4.1)
Short name:
AtPDIL1-3
Alternative name(s):
Protein disulfide isomerase 1
Short name:
AtPDI1
Protein disulfide isomerase-like 2-1
Short name:
AtPDIL2-1
Gene namesi
Name:PDIL1-3
Synonyms:PDI1, PDI72, PDIL2-1
Ordered Locus Names:At3g54960
ORF Names:F28P10.60, T15C9.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G54960.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum lumen Source: UniProtKB-SubCell
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_500006591726 – 579Protein disulfide isomerase-like 1-3Add BLAST554

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi27N-linked (GlcNAc...)Sequence analysis1
Glycosylationi108N-linked (GlcNAc...)Sequence analysis1
Glycosylationi115N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi128 ↔ 131Redox-activePROSITE-ProRule annotation
Glycosylationi209N-linked (GlcNAc...)Sequence analysis1
Glycosylationi293N-linked (GlcNAc...)Sequence analysis1
Glycosylationi313N-linked (GlcNAc...)Sequence analysis1
Glycosylationi338N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi467 ↔ 470Redox-activePROSITE-ProRule annotation
Glycosylationi520N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8VX13.
PRIDEiQ8VX13.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Inductioni

By chemically-induced ER stress response.1 Publication

Gene expression databases

GenevisibleiQ8VX13. AT.

Interactioni

Protein-protein interaction databases

BioGridi9977. 1 interactor.
IntActiQ8VX13. 1 interactor.
STRINGi3702.AT3G54960.1.

Structurei

3D structure databases

ProteinModelPortaliQ8VX13.
SMRiQ8VX13.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 204Thioredoxin 1PROSITE-ProRule annotationAdd BLAST124
Domaini416 – 546Thioredoxin 2PROSITE-ProRule annotationAdd BLAST131

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi576 – 579Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 2 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
HOGENOMiHOG000162459.
InParanoidiQ8VX13.
KOiK09580.
OMAiANESEKH.
OrthoDBiEOG093607A9.
PhylomeDBiQ8VX13.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VX13-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSSTSISL LLFVSFILLL VNSRAENASS GSDLDEELAF LAAEESKEQS
60 70 80 90 100
HGGGSYHEEE HDHQHRDFEN YDDLEQGGGE FHHGDHGYEE EPLPPVDEKD
110 120 130 140 150
VAVLTKDNFT EFVGNNSFAM VEFYAPWCGA CQALTPEYAA AATELKGLAA
160 170 180 190 200
LAKIDATEEG DLAQKYEIQG FPTVFLFVDG EMRKTYEGER TKDGIVTWLK
210 220 230 240 250
KKASPSIHNI TTKEEAERVL SAEPKLVFGF LNSLVGSESE ELAAASRLED
260 270 280 290 300
DLSFYQTASP DIAKLFEIET QVKRPALVLL KKEEEKLARF DGNFTKTAIA
310 320 330 340 350
EFVSANKVPL VINFTREGAS LIFESSVKNQ LILFAKANES EKHLPTLREV
360 370 380 390 400
AKSFKGKFVF VYVQMDNEDY GEAVSGFFGV TGAAPKVLVY TGNEDMRKFI
410 420 430 440 450
LDGELTVNNI KTLAEDFLAD KLKPFYKSDP LPENNDGDVK VIVGNNFDEI
460 470 480 490 500
VLDESKDVLL EIYAPWCGHC QSFEPIYNKL GKYLKGIDSL VVAKMDGTSN
510 520 530 540 550
EHPRAKADGF PTILFFPGGN KSFDPIAVDV DRTVVELYKF LKKHASIPFK
560 570
LEKPATPEPV ISTMKSDEKI EGDSSKDEL
Length:579
Mass (Da):64,212
Last modified:March 1, 2002 - v1
Checksum:i0DF50E500B24B829
GO
Isoform 2 (identifier: Q8VX13-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     507-518: ADGFPTILFFPG → VIKKKELRKSFW
     519-579: Missing.

Note: No experimental confirmation available.
Show »
Length:518
Mass (Da):57,691
Checksum:iB3AA1BB3BAFE49FF
GO

Sequence cautioni

The sequence CAB41088 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_039979507 – 518ADGFP…LFFPG → VIKKKELRKSFW in isoform 2. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_039980519 – 579Missing in isoform 2. 1 PublicationAdd BLAST61

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK318844 mRNA. Translation: BAH56959.1.
AJ271376 mRNA. Translation: CAC81067.1.
AL049655 Genomic DNA. Translation: CAB41088.1. Sequence problems.
AL132970 Genomic DNA. No translation available.
CP002686 Genomic DNA. Translation: AEE79318.1.
CP002686 Genomic DNA. Translation: AEE79319.1.
AY093115 mRNA. Translation: AAM13114.1.
BT000172 mRNA. Translation: AAN15491.1.
PIRiT06724.
RefSeqiNP_001118842.1. NM_001125370.1. [Q8VX13-2]
NP_191056.2. NM_115353.5. [Q8VX13-1]
UniGeneiAt.22358.

Genome annotation databases

EnsemblPlantsiAT3G54960.1; AT3G54960.1; AT3G54960. [Q8VX13-1]
GeneIDi824661.
GrameneiAT3G54960.1; AT3G54960.1; AT3G54960.
KEGGiath:AT3G54960.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK318844 mRNA. Translation: BAH56959.1.
AJ271376 mRNA. Translation: CAC81067.1.
AL049655 Genomic DNA. Translation: CAB41088.1. Sequence problems.
AL132970 Genomic DNA. No translation available.
CP002686 Genomic DNA. Translation: AEE79318.1.
CP002686 Genomic DNA. Translation: AEE79319.1.
AY093115 mRNA. Translation: AAM13114.1.
BT000172 mRNA. Translation: AAN15491.1.
PIRiT06724.
RefSeqiNP_001118842.1. NM_001125370.1. [Q8VX13-2]
NP_191056.2. NM_115353.5. [Q8VX13-1]
UniGeneiAt.22358.

3D structure databases

ProteinModelPortaliQ8VX13.
SMRiQ8VX13.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9977. 1 interactor.
IntActiQ8VX13. 1 interactor.
STRINGi3702.AT3G54960.1.

Proteomic databases

PaxDbiQ8VX13.
PRIDEiQ8VX13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G54960.1; AT3G54960.1; AT3G54960. [Q8VX13-1]
GeneIDi824661.
GrameneiAT3G54960.1; AT3G54960.1; AT3G54960.
KEGGiath:AT3G54960.

Organism-specific databases

TAIRiAT3G54960.

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
HOGENOMiHOG000162459.
InParanoidiQ8VX13.
KOiK09580.
OMAiANESEKH.
OrthoDBiEOG093607A9.
PhylomeDBiQ8VX13.

Miscellaneous databases

PROiQ8VX13.

Gene expression databases

GenevisibleiQ8VX13. AT.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDI13_ARATH
AccessioniPrimary (citable) accession number: Q8VX13
Secondary accession number(s): B3H5N9, Q9SV44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.