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Protein

Geraniol 8-hydroxylase

Gene

CYP76B6

Organism
Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydroxylase involved in the biosynthesis of hydroxygeraniol, a precursor of the terpenoid indole alkaloids such as vinblastine and vincristine. Also able to hydroxylate in vitro nerol and to catalyze 3'-hydroxylation of the flavanone naringenin to form eriodictyol. No activity with apigenin, kaempferol, p-coumaric acid and ferulic acid as substrates.3 Publications

Miscellaneous

The recommended numbering of geraniol gives (6E)-8-hydroxygeraniol as the product rather than 10-hydroxygeraniol as used in most publications.

Catalytic activityi

Geraniol + NADPH + O2 = (6E)-8-hydroxygeraniol + NADP+ + H2O.2 Publications

Cofactori

hemeBy similarity

Kineticsi

Kcat is 3.15 min (-1) for naringenin. Kcat is 7.86 min (-1) for geraniol.
  1. KM=15.81 µM for geraniol1 Publication
  2. KM=58.39 µM for naringenin1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi436Iron (heme axial ligand)By similarity1

    GO - Molecular functioni

    GO - Biological processi

    • monoterpenoid biosynthetic process Source: UniProtKB

    Keywordsi

    Molecular functionMonooxygenase, Oxidoreductase
    LigandHeme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BRENDAi1.14.13.152. 1211.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Geraniol 8-hydroxylase (EC:1.14.13.152)
    Alternative name(s):
    Cytochrome P450 76B6
    Geraniol 10-hydroxylase
    Short name:
    CrG10H
    Gene namesi
    Name:CYP76B6
    Synonyms:G10H
    OrganismiCatharanthus roseus (Madagascar periwinkle) (Vinca rosea)
    Taxonomic identifieri4058 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeCatharanthinaeCatharanthus

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 6LumenalSequence analysis6
    Transmembranei7 – 23HelicalSequence analysisAdd BLAST17
    Topological domaini24 – 493CytoplasmicSequence analysisAdd BLAST470

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004189191 – 493Geraniol 8-hydroxylaseAdd BLAST493

    Expressioni

    Tissue specificityi

    Expressed in roots, stems, leaves and flower buds. Hardly detected in mature flowers and fruits. Expressed in the internal phloem-associated parenchyma.1 Publication

    Inductioni

    Up-regulated by methyl jasmonate treatment.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VWZ7.
    SMRiQ8VWZ7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    KOiK15099.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiView protein in InterPro
    IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR036396. Cyt_P450_sf.
    PfamiView protein in Pfam
    PF00067. p450. 1 hit.
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiView protein in PROSITE
    PS00086. CYTOCHROME_P450. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8VWZ7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDYLTIILTL LFALTLYEAF SYLSRRTKNL PPGPSPLPFI GSLHLLGDQP
    60 70 80 90 100
    HKSLAKLSKK HGPIMSLKLG QITTIVISSS TMAKEVLQKQ DLAFSSRSVP
    110 120 130 140 150
    NALHAHNQFK FSVVWLPVAS RWRSLRKVLN SNIFSGNRLD ANQHLRTRKV
    160 170 180 190 200
    QELIAYCRKN SQSGEAVDVG RAAFRTSLNL LSNLIFSKDL TDPYSDSAKE
    210 220 230 240 250
    FKDLVWNIMV EAGKPNLVDF FPLLEKVDPQ GIRHRMTIHF GEVLKLFGGL
    260 270 280 290 300
    VNERLEQRRS KGEKNDVLDV LLTTSQESPE EIDRTHIERM CLDLFVAGTD
    310 320 330 340 350
    TTSSTLEWAM SEMLKNPDKM KKTQDELAQV IGRGKTIEES DINRLPYLRC
    360 370 380 390 400
    VMKETLRIHP PVPFLIPRKV EQSVEVCGYN VPKGSQVLVN AWAIGRDETV
    410 420 430 440 450
    WDDALAFKPE RFMESELDIR GRDFELIPFG AGRRICPGLP LALRTVPLML
    460 470 480 490
    GSLLNSFNWK LEGGMAPKDL DMEEKFGITL QKAHPLRAVP STL
    Length:493
    Mass (Da):55,658
    Last modified:March 1, 2002 - v1
    Checksum:i45781801A2A5A86A
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ251269 mRNA. Translation: CAC80883.1.

    Genome annotation databases

    KEGGiag:CAC80883.

    Similar proteinsi

    Entry informationi

    Entry nameiC76B6_CATRO
    AccessioniPrimary (citable) accession number: Q8VWZ7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: March 1, 2002
    Last modified: October 25, 2017
    This is version 78 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families