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Q8VWN6

- RFS_PEA

UniProt

Q8VWN6 - RFS_PEA

Protein

Galactinol--sucrose galactosyltransferase

Gene

RFS

Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers. Able to utilize D-ononitol and D-pinitol as acceptors. May also act as a glycoside hydrolase.1 Publication

    Catalytic activityi

    Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.1 Publication

    Enzyme regulationi

    Inhibited by 1-deoxygalactonojirimycin. Not inhibited by stachyose. Strong inhibition of the hydrolytic activity by sucrose.1 Publication

    Kineticsi

    partially purified enzyme.

    1. KM=7.3 mM for galactinol for the raffinose synthesis activity1 Publication
    2. KM=22.9 mM for sucrose for the raffinose synthesis activity1 Publication
    3. KM=1.0 mM for galactinol for the galactinol hydrolysis activity1 Publication

    Vmax=199.2 pmol/sec/mg enzyme for the raffinose synthesis activity1 Publication

    Vmax=27.3 pmol/sec/mg enzyme for the galactinol hydrolysis activity1 Publication

    pH dependencei

    Optimum pH is 7.0.1 Publication

    GO - Molecular functioni

    1. galactinol-sucrose galactosyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12496.
    BRENDAi2.4.1.82. 4872.

    Protein family/group databases

    CAZyiGH36. Glycoside Hydrolase Family 36.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Galactinol--sucrose galactosyltransferase (EC:2.4.1.82)
    Alternative name(s):
    Raffinose synthase
    Gene namesi
    Name:RFS
    OrganismiPisum sativum (Garden pea)
    Taxonomic identifieri3888 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 798798Galactinol--sucrose galactosyltransferasePRO_0000389254Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VWN6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi53 – 597Poly-Thr
    Compositional biasi75 – 784Poly-Asn
    Compositional biasi281 – 2844Poly-Asp

    Sequence similaritiesi

    Belongs to the glycosyl hydrolases 36 family.Curated

    Family and domain databases

    Gene3Di3.20.20.70. 2 hits.
    InterProiIPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR008811. Glycosyl_hydrolases_36.
    [Graphical view]
    PfamiPF05691. Raffinose_syn. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    Q8VWN6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPPSITKTA TQQDVISTVD IGNSPLLSIS LDQSRNFLVN GHPFLTQVPP    50
    NITTTTTSTP SPFLDFKSNK DTIANNNNTL QQQGCFVGFN TTEAKSHHVV 100
    PLGKLKGIKF TSIFRFKVWW TTHWVGTNGH ELQHETQILI LDKNISLGRP 150
    YVLLLPILEN SFRTSLQPGL NDYVDMSVES GSTHVTGSTF KACLYLHLSN 200
    DPYRLVKEAV KVIQTKLGTF KTLEEKTPPS IIEKFGWCTW DAFYLKVHPK 250
    GVWEGVKALT DGGCPPGFVI IDDGWQSISH DDDDPVTERD GMNRTSAGEQ 300
    MPCRLIKYEE NYKFREYENG DNGGKKGLVG FVRDLKEEFR SVESVYVWHA 350
    LCGYWGGVRP KVCGMPEAKV VVPKLSPGVK MTMEDLAVDK IVENGVGLVP 400
    PNLAQEMFDG IHSHLESAGI DGVKVDVIHL LELLSEEYGG RVELAKAYYK 450
    ALTSSVNKHF KGNGVIASME HCNDFFLLGT EAISLGRVGD DFWCCDPSGD 500
    PNGTYWLQGC HMVHCAYNSL WMGNFIHPDW DMFQSTHPCA EFHAASRAIS 550
    GGPVYVSDCV GNHNFKLLKS FVLPDGSILR CQHYALPTRD CLFEDPLHNG 600
    KTMLKIWNLN KYAGVLGLFN CQGGGWCPET RRNKSASEFS HAVTCYASPE 650
    DIEWCNGKTP MDIKGVDVFA VYFFKEKKLS LMKCSDRLEV SLEPFSFELM 700
    TVSPLKVFSK RLIQFAPIGL VNMLNSGGAV QSLEFDDSAS LVKIGVRGCG 750
    ELSVFASEKP VCCKIDGVSV EFDYEDKMVR VQILWPGSST LSLVEFLF 798
    Length:798
    Mass (Da):88,717
    Last modified:March 1, 2002 - v1
    Checksum:i8D3F3ED5BF8617B7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ426475 mRNA. Translation: CAD20127.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ426475 mRNA. Translation: CAD20127.2 .

    3D structure databases

    ProteinModelPortali Q8VWN6.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH36. Glycoside Hydrolase Family 36.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-12496.
    BRENDAi 2.4.1.82. 4872.

    Family and domain databases

    Gene3Di 3.20.20.70. 2 hits.
    InterProi IPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR008811. Glycosyl_hydrolases_36.
    [Graphical view ]
    Pfami PF05691. Raffinose_syn. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Functional expression of a cDNA encoding pea (Pisum sativum L.) raffinose synthase, partial purification of the enzyme from maturing seeds, and steady-state kinetic analysis of raffinose synthesis."
      Peterbauer T., Mach L., Mucha J., Richter A.
      Planta 215:839-846(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: cv. Kelvedon Wonder.
      Tissue: Seed.

    Entry informationi

    Entry nameiRFS_PEA
    AccessioniPrimary (citable) accession number: Q8VWN6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3