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Q8VWN6

- RFS_PEA

UniProt

Q8VWN6 - RFS_PEA

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Protein
Galactinol--sucrose galactosyltransferase
Gene
RFS
Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers. Able to utilize D-ononitol and D-pinitol as acceptors. May also act as a glycoside hydrolase.1 Publication

Catalytic activityi

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.1 Publication

Enzyme regulationi

Inhibited by 1-deoxygalactonojirimycin. Not inhibited by stachyose. Strong inhibition of the hydrolytic activity by sucrose.1 Publication

Kineticsi

partially purified enzyme.

  1. KM=7.3 mM for galactinol for the raffinose synthesis activity1 Publication
  2. KM=22.9 mM for sucrose for the raffinose synthesis activity
  3. KM=1.0 mM for galactinol for the galactinol hydrolysis activity

Vmax=199.2 pmol/sec/mg enzyme for the raffinose synthesis activity

Vmax=27.3 pmol/sec/mg enzyme for the galactinol hydrolysis activity

pH dependencei

Optimum pH is 7.0.

GO - Molecular functioni

  1. galactinol-sucrose galactosyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12496.
BRENDAi2.4.1.82. 4872.

Protein family/group databases

CAZyiGH36. Glycoside Hydrolase Family 36.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactinol--sucrose galactosyltransferase (EC:2.4.1.82)
Alternative name(s):
Raffinose synthase
Gene namesi
Name:RFS
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 798798Galactinol--sucrose galactosyltransferase
PRO_0000389254Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ8VWN6.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi53 – 597Poly-Thr
Compositional biasi75 – 784Poly-Asn
Compositional biasi281 – 2844Poly-Asp

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view]
PfamiPF05691. Raffinose_syn. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 3 hits.

Sequencei

Sequence statusi: Complete.

Q8VWN6-1 [UniParc]FASTAAdd to Basket

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MAPPSITKTA TQQDVISTVD IGNSPLLSIS LDQSRNFLVN GHPFLTQVPP    50
NITTTTTSTP SPFLDFKSNK DTIANNNNTL QQQGCFVGFN TTEAKSHHVV 100
PLGKLKGIKF TSIFRFKVWW TTHWVGTNGH ELQHETQILI LDKNISLGRP 150
YVLLLPILEN SFRTSLQPGL NDYVDMSVES GSTHVTGSTF KACLYLHLSN 200
DPYRLVKEAV KVIQTKLGTF KTLEEKTPPS IIEKFGWCTW DAFYLKVHPK 250
GVWEGVKALT DGGCPPGFVI IDDGWQSISH DDDDPVTERD GMNRTSAGEQ 300
MPCRLIKYEE NYKFREYENG DNGGKKGLVG FVRDLKEEFR SVESVYVWHA 350
LCGYWGGVRP KVCGMPEAKV VVPKLSPGVK MTMEDLAVDK IVENGVGLVP 400
PNLAQEMFDG IHSHLESAGI DGVKVDVIHL LELLSEEYGG RVELAKAYYK 450
ALTSSVNKHF KGNGVIASME HCNDFFLLGT EAISLGRVGD DFWCCDPSGD 500
PNGTYWLQGC HMVHCAYNSL WMGNFIHPDW DMFQSTHPCA EFHAASRAIS 550
GGPVYVSDCV GNHNFKLLKS FVLPDGSILR CQHYALPTRD CLFEDPLHNG 600
KTMLKIWNLN KYAGVLGLFN CQGGGWCPET RRNKSASEFS HAVTCYASPE 650
DIEWCNGKTP MDIKGVDVFA VYFFKEKKLS LMKCSDRLEV SLEPFSFELM 700
TVSPLKVFSK RLIQFAPIGL VNMLNSGGAV QSLEFDDSAS LVKIGVRGCG 750
ELSVFASEKP VCCKIDGVSV EFDYEDKMVR VQILWPGSST LSLVEFLF 798
Length:798
Mass (Da):88,717
Last modified:March 1, 2002 - v1
Checksum:i8D3F3ED5BF8617B7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ426475 mRNA. Translation: CAD20127.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ426475 mRNA. Translation: CAD20127.2 .

3D structure databases

ProteinModelPortali Q8VWN6.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH36. Glycoside Hydrolase Family 36.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-12496.
BRENDAi 2.4.1.82. 4872.

Family and domain databases

Gene3Di 3.20.20.70. 2 hits.
InterProi IPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view ]
Pfami PF05691. Raffinose_syn. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 3 hits.
ProtoNeti Search...

Publicationsi

  1. "Functional expression of a cDNA encoding pea (Pisum sativum L.) raffinose synthase, partial purification of the enzyme from maturing seeds, and steady-state kinetic analysis of raffinose synthesis."
    Peterbauer T., Mach L., Mucha J., Richter A.
    Planta 215:839-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: cv. Kelvedon Wonder.
    Tissue: Seed.

Entry informationi

Entry nameiRFS_PEA
AccessioniPrimary (citable) accession number: Q8VWN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: March 1, 2002
Last modified: February 19, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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