Q8VWN6 (RFS_PEA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactinol--sucrose galactosyltransferase
EC=2.4.1.82

Alternative name(s):
Raffinose synthase

Gene names

Name:

RFS

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	798 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers. Able to utilize D-ononitol and D-pinitol as acceptors. May also act as a glycoside hydrolase. Ref.1
Catalytic activity	Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose. Ref.1
Enzyme regulation	Inhibited by 1-deoxygalactonojirimycin. Not inhibited by stachyose. Strong inhibition of the hydrolytic activity by sucrose. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolases 36 family.
Biophysicochemical properties	Kinetic parameters: partially purified enzyme. KM=7.3 mM for galactinol for the raffinose synthesis activity Ref.1 KM=22.9 mM for sucrose for the raffinose synthesis activity KM=1.0 mM for galactinol for the galactinol hydrolysis activity Vmax=199.2 pmol/sec/mg enzyme for the raffinose synthesis activity Vmax=27.3 pmol/sec/mg enzyme for the galactinol hydrolysis activity pH dependence: Optimum pH is 7.0.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	galactinol-sucrose galactosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 798	798	Galactinol--sucrose galactosyltransferase		PRO_0000389254
Regions
	Compositional bias	53 – 59	7	Poly-Thr
	Compositional bias	75 – 78	4	Poly-Asn
	Compositional bias	281 – 284	4	Poly-Asp

Sequences

	Sequence		Length	Mass (Da)
	Q8VWN6 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 8D3F3ED5BF8617B7	FASTA	798	88,717
	10 20 30 40 50 60 MAPPSITKTA TQQDVISTVD IGNSPLLSIS LDQSRNFLVN GHPFLTQVPP NITTTTTSTP 70 80 90 100 110 120 SPFLDFKSNK DTIANNNNTL QQQGCFVGFN TTEAKSHHVV PLGKLKGIKF TSIFRFKVWW 130 140 150 160 170 180 TTHWVGTNGH ELQHETQILI LDKNISLGRP YVLLLPILEN SFRTSLQPGL NDYVDMSVES 190 200 210 220 230 240 GSTHVTGSTF KACLYLHLSN DPYRLVKEAV KVIQTKLGTF KTLEEKTPPS IIEKFGWCTW 250 260 270 280 290 300 DAFYLKVHPK GVWEGVKALT DGGCPPGFVI IDDGWQSISH DDDDPVTERD GMNRTSAGEQ 310 320 330 340 350 360 MPCRLIKYEE NYKFREYENG DNGGKKGLVG FVRDLKEEFR SVESVYVWHA LCGYWGGVRP 370 380 390 400 410 420 KVCGMPEAKV VVPKLSPGVK MTMEDLAVDK IVENGVGLVP PNLAQEMFDG IHSHLESAGI 430 440 450 460 470 480 DGVKVDVIHL LELLSEEYGG RVELAKAYYK ALTSSVNKHF KGNGVIASME HCNDFFLLGT 490 500 510 520 530 540 EAISLGRVGD DFWCCDPSGD PNGTYWLQGC HMVHCAYNSL WMGNFIHPDW DMFQSTHPCA 550 560 570 580 590 600 EFHAASRAIS GGPVYVSDCV GNHNFKLLKS FVLPDGSILR CQHYALPTRD CLFEDPLHNG 610 620 630 640 650 660 KTMLKIWNLN KYAGVLGLFN CQGGGWCPET RRNKSASEFS HAVTCYASPE DIEWCNGKTP 670 680 690 700 710 720 MDIKGVDVFA VYFFKEKKLS LMKCSDRLEV SLEPFSFELM TVSPLKVFSK RLIQFAPIGL 730 740 750 760 770 780 VNMLNSGGAV QSLEFDDSAS LVKIGVRGCG ELSVFASEKP VCCKIDGVSV EFDYEDKMVR 790 VQILWPGSST LSLVEFLF

References

[1]

"Functional expression of a cDNA encoding pea (Pisum sativum L.) raffinose synthase, partial purification of the enzyme from maturing seeds, and steady-state kinetic analysis of raffinose synthesis." Peterbauer T., Mach L., Mucha J., Richter A. Planta 215:839-846(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: cv. Kelvedon Wonder. Tissue: Seed.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ426475 mRNA. Translation: CAD20127.2.
3D structure databases
ProteinModelPortal	Q8VWN6.
ModBase	Search...
Protein family/group databases
CAZy	GH36. Glycoside Hydrolase Family 36.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-12496.
BRENDA	2.4.1.82. 4872.
Family and domain databases
Gene3D	3.20.20.70. 2 hits.
InterPro	IPR013785. Aldolase_TIM. IPR017853. Glycoside_hydrolase_SF. IPR008811. Raffinose_synthase. [Graphical view]
Pfam	PF05691. Raffinose_syn. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RFS_PEA

Accession

Primary (citable) accession number: Q8VWN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 24, 2009
Last sequence update:	March 1, 2002
Last modified:	March 6, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program