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Q8VWN6 (RFS_PEA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Galactinol--sucrose galactosyltransferase

EC=2.4.1.82
Alternative name(s):
Raffinose synthase
Gene names
Name:RFS
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Protein attributes

Sequence length798 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers. Able to utilize D-ononitol and D-pinitol as acceptors. May also act as a glycoside hydrolase. Ref.1

Catalytic activity

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose. Ref.1

Enzyme regulation

Inhibited by 1-deoxygalactonojirimycin. Not inhibited by stachyose. Strong inhibition of the hydrolytic activity by sucrose. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolases 36 family.

Biophysicochemical properties

Kinetic parameters:

partially purified enzyme.

KM=7.3 mM for galactinol for the raffinose synthesis activity Ref.1

KM=22.9 mM for sucrose for the raffinose synthesis activity

KM=1.0 mM for galactinol for the galactinol hydrolysis activity

Vmax=199.2 pmol/sec/mg enzyme for the raffinose synthesis activity

Vmax=27.3 pmol/sec/mg enzyme for the galactinol hydrolysis activity

pH dependence:

Optimum pH is 7.0.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Molecular functionGlycosyltransferase
Transferase
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functiongalactinol-sucrose galactosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 798798Galactinol--sucrose galactosyltransferase
PRO_0000389254

Regions

Compositional bias53 – 597Poly-Thr
Compositional bias75 – 784Poly-Asn
Compositional bias281 – 2844Poly-Asp

Sequences

Sequence LengthMass (Da)Tools
Q8VWN6 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 8D3F3ED5BF8617B7

FASTA79888,717
        10         20         30         40         50         60 
MAPPSITKTA TQQDVISTVD IGNSPLLSIS LDQSRNFLVN GHPFLTQVPP NITTTTTSTP 

        70         80         90        100        110        120 
SPFLDFKSNK DTIANNNNTL QQQGCFVGFN TTEAKSHHVV PLGKLKGIKF TSIFRFKVWW 

       130        140        150        160        170        180 
TTHWVGTNGH ELQHETQILI LDKNISLGRP YVLLLPILEN SFRTSLQPGL NDYVDMSVES 

       190        200        210        220        230        240 
GSTHVTGSTF KACLYLHLSN DPYRLVKEAV KVIQTKLGTF KTLEEKTPPS IIEKFGWCTW 

       250        260        270        280        290        300 
DAFYLKVHPK GVWEGVKALT DGGCPPGFVI IDDGWQSISH DDDDPVTERD GMNRTSAGEQ 

       310        320        330        340        350        360 
MPCRLIKYEE NYKFREYENG DNGGKKGLVG FVRDLKEEFR SVESVYVWHA LCGYWGGVRP 

       370        380        390        400        410        420 
KVCGMPEAKV VVPKLSPGVK MTMEDLAVDK IVENGVGLVP PNLAQEMFDG IHSHLESAGI 

       430        440        450        460        470        480 
DGVKVDVIHL LELLSEEYGG RVELAKAYYK ALTSSVNKHF KGNGVIASME HCNDFFLLGT 

       490        500        510        520        530        540 
EAISLGRVGD DFWCCDPSGD PNGTYWLQGC HMVHCAYNSL WMGNFIHPDW DMFQSTHPCA 

       550        560        570        580        590        600 
EFHAASRAIS GGPVYVSDCV GNHNFKLLKS FVLPDGSILR CQHYALPTRD CLFEDPLHNG 

       610        620        630        640        650        660 
KTMLKIWNLN KYAGVLGLFN CQGGGWCPET RRNKSASEFS HAVTCYASPE DIEWCNGKTP 

       670        680        690        700        710        720 
MDIKGVDVFA VYFFKEKKLS LMKCSDRLEV SLEPFSFELM TVSPLKVFSK RLIQFAPIGL 

       730        740        750        760        770        780 
VNMLNSGGAV QSLEFDDSAS LVKIGVRGCG ELSVFASEKP VCCKIDGVSV EFDYEDKMVR 

       790 
VQILWPGSST LSLVEFLF 

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References

[1]"Functional expression of a cDNA encoding pea (Pisum sativum L.) raffinose synthase, partial purification of the enzyme from maturing seeds, and steady-state kinetic analysis of raffinose synthesis."
Peterbauer T., Mach L., Mucha J., Richter A.
Planta 215:839-846(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: cv. Kelvedon Wonder.
Tissue: Seed.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ426475 mRNA. Translation: CAD20127.2.

3D structure databases

ProteinModelPortalQ8VWN6.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH36. Glycoside Hydrolase Family 36.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-12496.
BRENDA2.4.1.82. 4872.

Family and domain databases

Gene3D3.20.20.70. 2 hits.
InterProIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view]
PfamPF05691. Raffinose_syn. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 3 hits.
ProtoNetSearch...

Entry information

Entry nameRFS_PEA
AccessionPrimary (citable) accession number: Q8VWN6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: March 1, 2002
Last modified: February 19, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families