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Protein

Telomere repeat-binding factor 1

Gene

TRB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds preferentially double-stranded telomeric repeats.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi28 – 5730H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • activating transcription factor binding Source: TAIR
  • double-stranded telomeric DNA binding Source: TAIR
  • promoter-specific chromatin binding Source: TAIR
  • protein homodimerization activity Source: TAIR
  • repressing transcription factor binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • nucleosome assembly Source: InterPro
  • regulation of transcription from RNA polymerase II promoter Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to auxin Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to ethylene Source: TAIR
  • response to gibberellin Source: TAIR
  • response to jasmonic acid Source: TAIR
  • response to salicylic acid Source: TAIR
  • response to salt stress Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Telomere repeat-binding factor 1
Short name:
AtTRB1
Alternative name(s):
MYB transcription factor
Gene namesi
Name:TRB1
Ordered Locus Names:At1g49950
ORF Names:F2J10.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G49950.

Subcellular locationi

GO - Cellular componenti

  • chromatin Source: UniProtKB
  • nucleolus Source: UniProtKB
  • nucleosome Source: InterPro
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300Telomere repeat-binding factor 1PRO_0000417010Add
BLAST

Proteomic databases

PaxDbiQ8VWK4.
PRIDEiQ8VWK4.

PTM databases

iPTMnetiQ8VWK4.

Expressioni

Gene expression databases

GenevisibleiQ8VWK4. AT.

Interactioni

Subunit structurei

Forms a homodimer and heterodimers with TRB2 or TRB3. Interacts with POT1b, TRB2 and TRB3 through its H15 domain.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
POT1BQ6NKX54EBI-476101,EBI-476223
TRB2Q9FJW54EBI-476101,EBI-476115
TRB3Q9M2X33EBI-476101,EBI-476134

GO - Molecular functioni

  • activating transcription factor binding Source: TAIR
  • protein homodimerization activity Source: TAIR
  • repressing transcription factor binding Source: TAIR

Protein-protein interaction databases

BioGridi26643. 1 interaction.
IntActiQ8VWK4. 4 interactions.
MINTiMINT-1212432.
STRINGi3702.AT1G49950.1.

Structurei

3D structure databases

ProteinModelPortaliQ8VWK4.
SMRiQ8VWK4. Positions 117-184.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5858HTH myb-typePROSITE-ProRule annotationAdd
BLAST
Domaini117 – 18569H15PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili241 – 29050Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi242 – 28443Ala-richAdd
BLAST

Domaini

HTH myb-type domain confers double-stranded telomeric DNA-binding while the H15 domain is involved in non-specific DNA-protein interaction and multimerization.1 Publication

Sequence similaritiesi

Belongs to the histone H1/H5 family. SMH subfamily.PROSITE-ProRule annotation
Contains 1 H15 (linker histone H1/H5 globular) domain.PROSITE-ProRule annotation
Contains 1 HTH myb-type DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IHMF. Eukaryota.
ENOG4111SQS. LUCA.
HOGENOMiHOG000238013.
InParanoidiQ8VWK4.
KOiK09422.
OMAiCKMMIRA.
PhylomeDBiQ8VWK4.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.60. 1 hit.
InterProiIPR005818. Histone_H1/H5_H15.
IPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00538. Linker_histone. 1 hit.
PF00249. Myb_DNA-binding. 1 hit.
[Graphical view]
SMARTiSM00526. H15. 1 hit.
SM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF46785. SSF46785. 1 hit.
PROSITEiPS51504. H15. 1 hit.
PS51294. HTH_MYB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VWK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAPKQKWTQ EEESALKSGV IKHGPGKWRT ILKDPEFSGV LYLRSNVDLK
60 70 80 90 100
DKWRNMSVMA NGWGSREKSR LAVKRTFSLP KQEENSLALT NSLQSDEENV
110 120 130 140 150
DATSGLQVSS NPPPRRPNVR LDSLIMEAIA TLKEPGGCNK TTIGAYIEDQ
160 170 180 190 200
YHAPPDFKRL LSTKLKYLTS CGKLVKVKRK YRIPNSTPLS SHRRKGLGVF
210 220 230 240 250
GGKQRTSSLP SPKTDIDEVN FQTRSQIDTE IARMKSMNVH EAAAVAAQAV
260 270 280 290 300
AEAEAAMAEA EEAAKEAEAA EAEAEAAQAF AEEASKTLKG RNICKMMIRA
Length:300
Mass (Da):33,017
Last modified:March 1, 2002 - v1
Checksum:i3B4A383E129F537B
GO

Sequence cautioni

The sequence AAF76448.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti188 – 1881P → S in AAM65540 (Ref. 6) Curated
Sequence conflicti276 – 2761A → V in AAL73438 (Ref. 1) Curated
Sequence conflicti276 – 2761A → V in AAM65540 (Ref. 6) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83623 Genomic DNA. Translation: AAL73123.1.
U83624 mRNA. Translation: AAL73438.1.
AY519539 mRNA. Translation: AAS10009.1.
AC015445 Genomic DNA. Translation: AAF76448.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32497.1.
CP002684 Genomic DNA. Translation: AEE32498.1.
CP002684 Genomic DNA. Translation: AEE32499.1.
AY062736 mRNA. Translation: AAL32814.1.
BT009677 mRNA. Translation: AAP80178.1.
AY087994 mRNA. Translation: AAM65540.1.
RefSeqiNP_564559.1. NM_103882.3.
NP_849789.1. NM_179458.2.
NP_973998.1. NM_202269.2.
UniGeneiAt.27250.

Genome annotation databases

EnsemblPlantsiAT1G49950.1; AT1G49950.1; AT1G49950.
AT1G49950.2; AT1G49950.2; AT1G49950.
AT1G49950.3; AT1G49950.3; AT1G49950.
GeneIDi841418.
GrameneiAT1G49950.1; AT1G49950.1; AT1G49950.
AT1G49950.2; AT1G49950.2; AT1G49950.
AT1G49950.3; AT1G49950.3; AT1G49950.
KEGGiath:AT1G49950.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83623 Genomic DNA. Translation: AAL73123.1.
U83624 mRNA. Translation: AAL73438.1.
AY519539 mRNA. Translation: AAS10009.1.
AC015445 Genomic DNA. Translation: AAF76448.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32497.1.
CP002684 Genomic DNA. Translation: AEE32498.1.
CP002684 Genomic DNA. Translation: AEE32499.1.
AY062736 mRNA. Translation: AAL32814.1.
BT009677 mRNA. Translation: AAP80178.1.
AY087994 mRNA. Translation: AAM65540.1.
RefSeqiNP_564559.1. NM_103882.3.
NP_849789.1. NM_179458.2.
NP_973998.1. NM_202269.2.
UniGeneiAt.27250.

3D structure databases

ProteinModelPortaliQ8VWK4.
SMRiQ8VWK4. Positions 117-184.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi26643. 1 interaction.
IntActiQ8VWK4. 4 interactions.
MINTiMINT-1212432.
STRINGi3702.AT1G49950.1.

PTM databases

iPTMnetiQ8VWK4.

Proteomic databases

PaxDbiQ8VWK4.
PRIDEiQ8VWK4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G49950.1; AT1G49950.1; AT1G49950.
AT1G49950.2; AT1G49950.2; AT1G49950.
AT1G49950.3; AT1G49950.3; AT1G49950.
GeneIDi841418.
GrameneiAT1G49950.1; AT1G49950.1; AT1G49950.
AT1G49950.2; AT1G49950.2; AT1G49950.
AT1G49950.3; AT1G49950.3; AT1G49950.
KEGGiath:AT1G49950.

Organism-specific databases

TAIRiAT1G49950.

Phylogenomic databases

eggNOGiENOG410IHMF. Eukaryota.
ENOG4111SQS. LUCA.
HOGENOMiHOG000238013.
InParanoidiQ8VWK4.
KOiK09422.
OMAiCKMMIRA.
PhylomeDBiQ8VWK4.

Miscellaneous databases

PROiQ8VWK4.

Gene expression databases

GenevisibleiQ8VWK4. AT.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.60. 1 hit.
InterProiIPR005818. Histone_H1/H5_H15.
IPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00538. Linker_histone. 1 hit.
PF00249. Myb_DNA-binding. 1 hit.
[Graphical view]
SMARTiSM00526. H15. 1 hit.
SM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF46785. SSF46785. 1 hit.
PROSITEiPS51504. H15. 1 hit.
PS51294. HTH_MYB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and functional characterisation of the Arabidopsis telomere repeat binding factor TRB1."
    Sprenger M., Weisshaar B.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. Columbia and cv. Landsberg erecta.
  2. "The MYB transcription factor family in Arabidopsis: a genome-wide cloning and expression pattern analysis."
    Qu L.-J., Gu H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "The maize Single myb histone 1 gene, Smh1, belongs to a novel gene family and encodes a protein that binds telomere DNA repeats in vitro."
    Marian C.O., Bordoli S.J., Goltz M., Santarella R.A., Jackson L.P., Danilevskaya O., Beckstette M., Meeley R., Bass H.W.
    Plant Physiol. 133:1336-1350(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  8. "Interactions of putative telomere-binding proteins in Arabidopsis thaliana: identification of functional TRF2 homolog in plants."
    Kuchar M., Fajkus J.
    FEBS Lett. 578:311-315(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: HOMODIMERIZATION, INTERACTION WITH POT1B; TRB2 AND TRB3.
  9. "The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family."
    Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X., Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H., Qu L.-J.
    Plant Mol. Biol. 60:107-124(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  10. "Mapping of interaction domains of putative telomere-binding proteins AtTRB1 and AtPOT1b from Arabidopsis thaliana."
    Schrumpfova P.P., Kuchar M., Palecek J., Fajkus J.
    FEBS Lett. 582:1400-1406(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: HOMODIMERIZATION, INTERACTION WITH POT1B; TRB2 AND TRB3.
  11. "Functional characterization of domains in AtTRB1, a putative telomere-binding protein in Arabidopsis thaliana."
    Mozgova I., Schrumpfova P.P., Hofr C., Fajkus J.
    Phytochemistry 69:1814-1819(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: MULTIMERIZATION, DNA-BINDING, DOMAIN.
  12. "Single-Myb-histone proteins from Arabidopsis thaliana: a quantitative study of telomere-binding specificity and kinetics."
    Hofr C., Sultesova P., Zimmermann M., Mozgova I., Prochazkova Schrumpfova P., Wimmerova M., Fajkus J.
    Biochem. J. 419:221-228(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "AtTRB1, a telomeric DNA-binding protein from Arabidopsis, is concentrated in the nucleolus and shows highly dynamic association with chromatin."
    Dvorackova M., Rossignol P., Shaw P.J., Koroleva O.A., Doonan J.H., Fajkus J.
    Plant J. 61:637-649(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTRB1_ARATH
AccessioniPrimary (citable) accession number: Q8VWK4
Secondary accession number(s): Q8LA70, Q8VX39, Q9LPL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: March 1, 2002
Last modified: April 13, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.