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Q8VVE3 (RL10_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
50S ribosomal protein L10
Gene names
Name:rplJ
Ordered Locus Names:TTHA0209
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length173 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors Probable. HAMAP-Rule MF_00362

Subunit structure

Part of the 50S ribosomal subunit. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which 3 L12 dimers bind in a sequential fashion. Ref.4

Post-translational modification

The N-terminus is blocked. HAMAP-Rule MF_00362

Sequence similarities

Belongs to the ribosomal protein L10P family.

Caution

Note that in Ref.3 it was found that the N-terminus is blocked, whereas the mass determined in Ref.5 suggests that the initiator methionine is removed. No extra mass for a blocking group was found.

Mass spectrometry

Molecular mass is 96075±13 Da from positions 2 - 173. Determined by ESI. Isolated L10(L12)6. Ref.4

Molecular mass is 18434±1 Da from positions 2 - 173. Determined by ESI. Ref.4

Molecular mass is 18436 Da from positions 2 - 173. Determined by MALDI. Ref.5

Ontologies

Keywords
   LigandRNA-binding
rRNA-binding
   Molecular functionRibonucleoprotein
Ribosomal protein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processribosome biogenesis

Inferred from electronic annotation. Source: InterPro

translation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentribosome

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionLSU rRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 17317250S ribosomal protein L10 HAMAP-Rule MF_00362
PRO_0000154736

Experimental info

Sequence conflict127 – 1282EL → DV in CAD11986. Ref.1
Sequence conflict137 – 1382EL → DV in CAD11986. Ref.1

Secondary structure

.............................. 173
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8VVE3 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 266CF72B036C6D5F

FASTA17318,566
        10         20         30         40         50         60 
MPNKRNVELL ATLKENLERA QGSFFLVNYQ GLPAKETHAL RQALKQNGAR LFVAKNTLIR 

        70         80         90        100        110        120 
LALKELGLPE LDGLQGPSAV VFYEDPVAAA KTLVQFAKSN PKGIPQVKSG LLQGQILTAK 

       130        140        150        160        170 
DVEALAELPT MDELRAELVG VLQAPMAELV GVLGGVAREL VGILEAYAEK KAA 

« Hide

References

« Hide 'large scale' references
[1]"Ribosomal protein L12 and L10 from Thermus thermophilus, dissertation."
Huang Y., Sprinzl M.
Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
[3]"Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus."
Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T.
Biol. Chem. 381:1079-1087(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-11, BLOCKAGE OF N-TERMINUS.
[4]"Heptameric (L12)6/L10 rather than canonical pentameric complexes are found by tandem MS of intact ribosomes from thermophilic bacteria."
Ilag L.L., Videler H., McKay A.R., Sobott F., Fucini P., Nierhaus K.H., Robinson C.V.
Proc. Natl. Acad. Sci. U.S.A. 102:8192-8197(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, STOICHIOMETRY, MASS SPECTROMETRY.
[5]"Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry."
Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.
Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ419825 Genomic DNA. Translation: CAD11986.1.
AP008226 Genomic DNA. Translation: BAD70032.1.
RefSeqYP_143475.1. NC_006461.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2J01X-ray2.80J2-173[»]
2J03X-ray2.80J2-173[»]
3I8IX-ray3.10Y1-173[»]
3KIRX-ray3.30J1-173[»]
3KITX-ray3.30J1-173[»]
3KIWX-ray3.60J1-173[»]
3KIYX-ray3.60J1-173[»]
ProteinModelPortalQ8VVE3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING300852.TTHA0209.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD70032; BAD70032; BAD70032.
GeneID3168578.
KEGGttj:TTHA0209.
PATRIC23955359. VBITheThe93045_0207.

Phylogenomic databases

eggNOGCOG0244.
HOGENOMHOG000004852.
KOK02864.
OMAFERMSSA.
OrthoDBEOG6DNTDR.
PhylomeDBQ8VVE3.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-218-MONOMER.

Family and domain databases

HAMAPMF_00362. Ribosomal_L10.
InterProIPR022973. Ribosomal_L10.
IPR001790. Ribosomal_L10/acidic_P0.
[Graphical view]
PfamPF00466. Ribosomal_L10. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8VVE3.

Entry information

Entry nameRL10_THET8
AccessionPrimary (citable) accession number: Q8VVE3
Secondary accession number(s): Q5SLT4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 84 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references