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Protein

50S ribosomal protein L10

Gene

rplJ

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.Curated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L10
Gene namesi
Name:rplJ
Ordered Locus Names:TTHA0209
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001547362 – 17350S ribosomal protein L10Add BLAST172

Post-translational modificationi

The N-terminus is blocked.

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which 3 L12 dimers bind in a sequential fashion.1 Publication

Protein-protein interaction databases

STRINGi300852.TTHA0209.

Structurei

Secondary structure

1173
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 10Combined sources5
Turni11 – 16Combined sources6
Beta strandi17 – 22Combined sources6
Beta strandi25 – 27Combined sources3
Turni34 – 36Combined sources3
Beta strandi37 – 41Combined sources5
Turni42 – 45Combined sources4
Beta strandi46 – 51Combined sources6
Helixi57 – 62Combined sources6
Turni63 – 65Combined sources3
Beta strandi66 – 69Combined sources4
Beta strandi76 – 80Combined sources5
Turni88 – 91Combined sources4
Beta strandi93 – 97Combined sources5
Beta strandi99 – 101Combined sources3
Turni105 – 111Combined sources7
Beta strandi113 – 118Combined sources6
Helixi122 – 125Combined sources4
Beta strandi128 – 131Combined sources4
Turni136 – 138Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V51X-ray2.80J2-173[»]
4V6FX-ray3.10DY1-173[»]
4V7JX-ray3.30AJ/BJ1-173[»]
4V7KX-ray3.60AJ/BJ1-173[»]
4W2EX-ray2.90J1-173[»]
4WPOX-ray2.80AK/CK1-173[»]
4WQFX-ray2.80AK/CK1-173[»]
4WQUX-ray2.80AK/CK1-173[»]
4WQYX-ray2.80AK/CK1-173[»]
5A9Zelectron microscopy4.70AI4-156[»]
5AA0electron microscopy5.00AI4-156[»]
5HAUX-ray3.001J/2J1-173[»]
5IMQelectron microscopy3.8021-173[»]
5IMRelectron microscopy-21-173[»]
5J8BX-ray2.60J1-173[»]
ProteinModelPortaliQ8VVE3.
SMRiQ8VVE3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8VVE3.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L10P family.Curated

Phylogenomic databases

eggNOGiENOG4108VZM. Bacteria.
COG0244. LUCA.
HOGENOMiHOG000004852.
KOiK02864.
OMAiSSMKICK.
PhylomeDBiQ8VVE3.

Family and domain databases

HAMAPiMF_00362. Ribosomal_L10. 1 hit.
InterProiIPR022973. Ribosomal_L10.
IPR001790. Ribosomal_L10P.
[Graphical view]
PfamiPF00466. Ribosomal_L10. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VVE3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNKRNVELL ATLKENLERA QGSFFLVNYQ GLPAKETHAL RQALKQNGAR
60 70 80 90 100
LFVAKNTLIR LALKELGLPE LDGLQGPSAV VFYEDPVAAA KTLVQFAKSN
110 120 130 140 150
PKGIPQVKSG LLQGQILTAK DVEALAELPT MDELRAELVG VLQAPMAELV
160 170
GVLGGVAREL VGILEAYAEK KAA
Length:173
Mass (Da):18,566
Last modified:January 23, 2007 - v4
Checksum:i266CF72B036C6D5F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti127 – 128EL → DV in CAD11986 (Ref. 1) Curated2
Sequence conflicti137 – 138EL → DV in CAD11986 (Ref. 1) Curated2

Mass spectrometryi

Molecular mass is 96075±13 Da from positions 2 - 173. Determined by ESI. Isolated L10(L12)6.1 Publication
Molecular mass is 18434±1 Da from positions 2 - 173. Determined by ESI. 1 Publication
Molecular mass is 18436 Da from positions 2 - 173. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ419825 Genomic DNA. Translation: CAD11986.1.
AP008226 Genomic DNA. Translation: BAD70032.1.
RefSeqiWP_008630583.1. NC_006461.1.
YP_143475.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70032; BAD70032; BAD70032.
GeneIDi3168578.
KEGGittj:TTHA0209.
PATRICi23955359. VBITheThe93045_0207.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ419825 Genomic DNA. Translation: CAD11986.1.
AP008226 Genomic DNA. Translation: BAD70032.1.
RefSeqiWP_008630583.1. NC_006461.1.
YP_143475.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V51X-ray2.80J2-173[»]
4V6FX-ray3.10DY1-173[»]
4V7JX-ray3.30AJ/BJ1-173[»]
4V7KX-ray3.60AJ/BJ1-173[»]
4W2EX-ray2.90J1-173[»]
4WPOX-ray2.80AK/CK1-173[»]
4WQFX-ray2.80AK/CK1-173[»]
4WQUX-ray2.80AK/CK1-173[»]
4WQYX-ray2.80AK/CK1-173[»]
5A9Zelectron microscopy4.70AI4-156[»]
5AA0electron microscopy5.00AI4-156[»]
5HAUX-ray3.001J/2J1-173[»]
5IMQelectron microscopy3.8021-173[»]
5IMRelectron microscopy-21-173[»]
5J8BX-ray2.60J1-173[»]
ProteinModelPortaliQ8VVE3.
SMRiQ8VVE3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0209.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70032; BAD70032; BAD70032.
GeneIDi3168578.
KEGGittj:TTHA0209.
PATRICi23955359. VBITheThe93045_0207.

Phylogenomic databases

eggNOGiENOG4108VZM. Bacteria.
COG0244. LUCA.
HOGENOMiHOG000004852.
KOiK02864.
OMAiSSMKICK.
PhylomeDBiQ8VVE3.

Miscellaneous databases

EvolutionaryTraceiQ8VVE3.

Family and domain databases

HAMAPiMF_00362. Ribosomal_L10. 1 hit.
InterProiIPR022973. Ribosomal_L10.
IPR001790. Ribosomal_L10P.
[Graphical view]
PfamiPF00466. Ribosomal_L10. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRL10_THET8
AccessioniPrimary (citable) accession number: Q8VVE3
Secondary accession number(s): Q5SLT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 98 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Note that in PubMed:11154066 it was found that the N-terminus is blocked, whereas the mass determined in PubMed:16287167 suggests that the initiator methionine is removed. No extra mass for a blocking group was found.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.