Q8VVE3 (RL10_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L10

Gene names

Name:	rplJ
Ordered Locus Names:	TTHA0209

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	173 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Part of the 50S ribosomal subunit.
Post-translational modification	The N-terminus is blocked. HAMAP-Rule MF_00362
Sequence similarities	Belongs to the ribosomal protein L10P family.
Caution	Note that in Ref.3 it was found that the N-terminus is blocked, whereas the mass determined in Ref.4 suggests that the initiator methionine is removed. No extra mass for a blocking group was found.
Mass spectrometry	Molecular mass is 18436 Da from positions 2 - 173. Determined by MALDI. Ref.4

Ontologies

Keywords
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ribosome biogenesis Inferred from electronic annotation. Source: InterPro translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3

Chain

2 – 173

172

50S ribosomal protein L10 HAMAP-Rule MF_00362

PRO_0000154736

Experimental info

Sequence conflict

127 – 128

2

EL → DV in CAD11986. Ref.1

Sequence conflict

137 – 138

2

EL → DV in CAD11986. Ref.1

Secondary structure

1

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173

Helix Strand Turn

Details...

Helix

8 – 12

5

Turn

13 – 18

6

Beta strand

22 – 24

3

Beta strand

35 – 37

3

Beta strand

43 – 46

4

Helix

57 – 62

6

Turn

63 – 65

3

Beta strand

66 – 70

5

Beta strand

76 – 80

5

Helix

88 – 93

6

Turn

94 – 98

5

Beta strand

99 – 101

3

Beta strand

103 – 105

3

Beta strand

113 – 116

4

Beta strand

118 – 121

4

Helix

122 – 125

4

Beta strand

126 – 128

3

Turn

136 – 138

3

Sequences

	Sequence		Length	Mass (Da)
	Q8VVE3 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 266CF72B036C6D5F	FASTA	173	18,566
	10 20 30 40 50 60 MPNKRNVELL ATLKENLERA QGSFFLVNYQ GLPAKETHAL RQALKQNGAR LFVAKNTLIR 70 80 90 100 110 120 LALKELGLPE LDGLQGPSAV VFYEDPVAAA KTLVQFAKSN PKGIPQVKSG LLQGQILTAK 130 140 150 160 170 DVEALAELPT MDELRAELVG VLQAPMAELV GVLGGVAREL VGILEAYAEK KAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ribosomal protein L12 and L10 from Thermus thermophilus, dissertation." Huang Y., Sprinzl M. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
[3]	"Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus." Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T. Biol. Chem. 381:1079-1087(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11, BLOCKAGE OF N-TERMINUS.
[4]	"Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry." Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A. Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ419825 Genomic DNA. Translation: CAD11986.1.
AP008226 Genomic DNA. Translation: BAD70032.1.

RefSeq

YP_143475.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2J01	X-ray	2.80	J	2-172	[»]
2J03	X-ray	2.80	J	2-172	[»]
3I8I	X-ray	3.10	Y	1-173	[»]
3KIR	X-ray	3.30	J	1-173	[»]
3KIT	X-ray	3.30	J	1-173	[»]
3KIW	X-ray	3.60	J	1-173	[»]
3KIY	X-ray	3.60	J	1-173	[»]

ProteinModelPortal

Q8VVE3.

ModBase

Search...

Protein-protein interaction databases

STRING

300852.TTHA0209.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAD70032; BAD70032; BAD70032.

GeneID

3168578.

KEGG

ttj:TTHA0209.

PATRIC

23955359. VBITheThe93045_0207.

Phylogenomic databases

eggNOG

COG0244.

HOGENOM

HOG000004852.

KO

K02864.

OMA

TLAHIAM.

ProtClustDB

PRK00099.

Family and domain databases

HAMAP

MF_00362. Ribosomal_L10.

InterPro

IPR022973. Ribosomal_L10.
IPR001790. Ribosomal_L10/acidic_P0.
[Graphical view]

PANTHER

PTHR11560:SF8. PTHR11560:SF8. 1 hit.

Pfam

PF00466. Ribosomal_L10. 1 hit.
[Graphical view]

PROSITE

PS01109. RIBOSOMAL_L10. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8VVE3.

Entry information

Entry name

RL10_THET8

Accession

Primary (citable) accession number: Q8VVE3
Secondary accession number(s): Q5SLT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 75 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program