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Q8VVE2

- RL7_THET8

UniProt

Q8VVE2 - RL7_THET8

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Protein
50S ribosomal protein L7/L12
Gene
rplL, TTHA0210
Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation. It extends beyond the surface of the 70S ribosome. The stalk is preferentially stabilized in 70S versus 50S crystals.UniRule annotation

GO - Molecular functioni

  1. structural constituent of ribosome Source: InterPro

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-219-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L7/L12
Gene namesi
Name:rplL
Ordered Locus Names:TTHA0210
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. ribosome Source: UniProtKB-KW
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 12512450S ribosomal protein L7/L12UniRule annotation
PRO_0000157597Add
BLAST

Post-translational modificationi

The ion at m/z 13016 is probably phosphorylated; treatment with phosphatase in the presence of kinase inhibitors decreases it weight. This form is only detected on 70S ribosomes and not in isolated ribosomal stalk complexes.1 Publication
The ion at m/z 12978 is suggested to be the N-acetylated form of the protein (L7). The ion at m/z 12964 is suggested to be consistent with dimethylation or formylation and is present in very small quantities in the sample.UniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homodimer. Part of the 50S ribosomal subunit; present in 6 copies per ribosome. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which 3 L12 dimers bind in a sequential fashion. Contacts protein L11.1 Publication

Protein-protein interaction databases

STRINGi300852.TTHA0210.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni2 – 76
Helixi9 – 135
Turni14 – 163
Helixi17 – 226
Turni25 – 273

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GIYX-ray5.50I/J32-125[»]
1YL3X-ray5.50I/J32-125[»]
2J01X-ray2.80L/M2-125[»]
2J03X-ray2.80L/M2-125[»]
3I8IX-ray3.10I/J1-125[»]
ProteinModelPortaliQ8VVE2.
SMRiQ8VVE2. Positions 3-125.

Miscellaneous databases

EvolutionaryTraceiQ8VVE2.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0222.
HOGENOMiHOG000248813.
KOiK02935.
OMAiEFISNMT.
OrthoDBiEOG6WMJ69.
PhylomeDBiQ8VVE2.

Family and domain databases

Gene3Di3.30.1390.10. 1 hit.
HAMAPiMF_00368. Ribosomal_L7_L12.
InterProiIPR000206. Ribosomal_L7/12.
IPR014719. Ribosomal_L7/12_C/ClpS-like.
IPR013823. Ribosomal_L7/L12_C.
IPR008932. Ribosomal_L7/L12_oligo.
[Graphical view]
PANTHERiPTHR11809. PTHR11809. 1 hit.
PfamiPF00542. Ribosomal_L12. 1 hit.
[Graphical view]
ProDomiPD001326. Ribosomal_L7/L12_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF48300. SSF48300. 1 hit.
SSF54736. SSF54736. 1 hit.
TIGRFAMsiTIGR00855. L12. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VVE2-1 [UniParc]FASTAAdd to Basket

« Hide

MALDIERIKE ELSQATVLEL KQLIDALKEA WGVTAAAPVA VAAAPAAGAA    50
AAPAEEKTEF DVILKEAGAK KLEVIKELRA ITGLGLKEAK DLAEKGGPVK 100
EGVSKQEAEE IKKKLEAVGA VVELK 125
Length:125
Mass (Da):13,067
Last modified:January 23, 2007 - v4
Checksum:i3919CBF76605AC87
GO

Mass spectrometryi

Molecular mass is 96075±13 Da from positions 2 - 125. Determined by ESI. Isolated L10(L12)6.1 Publication
Molecular mass is 77670±9 Da from positions 2 - 125. Determined by ESI. Isolated (L12)6.1 Publication
Molecular mass is 13016±1.9 Da from positions 2 - 125. Determined by ESI. Probably phosphorylated, more strongly associated with the 50S ribosomal subunit.1 Publication
Molecular mass is 12936±0.5 Da from positions 2 - 125. Determined by ESI. Not phosphorylated.1 Publication
Molecular mass is 12937 Da from positions 2 - 125. Determined by MALDI. 1 Publication
Molecular mass is 12964 Da from positions 2 - 125. Determined by MALDI. May be N-acetylated.1 Publication
Molecular mass is 12978 Da from positions 2 - 125. Determined by MALDI. May be dimethylated or formylated.1 Publication

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 782EL → DV in CAD11987. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ419825 Genomic DNA. Translation: CAD11987.1.
AP008226 Genomic DNA. Translation: BAD70033.1.
RefSeqiYP_143476.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70033; BAD70033; BAD70033.
GeneIDi3168576.
KEGGittj:TTHA0210.
PATRICi23955361. VBITheThe93045_0208.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ419825 Genomic DNA. Translation: CAD11987.1 .
AP008226 Genomic DNA. Translation: BAD70033.1 .
RefSeqi YP_143476.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GIY X-ray 5.50 I/J 32-125 [» ]
1YL3 X-ray 5.50 I/J 32-125 [» ]
2J01 X-ray 2.80 L/M 2-125 [» ]
2J03 X-ray 2.80 L/M 2-125 [» ]
3I8I X-ray 3.10 I/J 1-125 [» ]
ProteinModelPortali Q8VVE2.
SMRi Q8VVE2. Positions 3-125.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 300852.TTHA0210.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD70033 ; BAD70033 ; BAD70033 .
GeneIDi 3168576.
KEGGi ttj:TTHA0210.
PATRICi 23955361. VBITheThe93045_0208.

Phylogenomic databases

eggNOGi COG0222.
HOGENOMi HOG000248813.
KOi K02935.
OMAi EFISNMT.
OrthoDBi EOG6WMJ69.
PhylomeDBi Q8VVE2.

Enzyme and pathway databases

BioCyci TTHE300852:GH8R-219-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q8VVE2.

Family and domain databases

Gene3Di 3.30.1390.10. 1 hit.
HAMAPi MF_00368. Ribosomal_L7_L12.
InterProi IPR000206. Ribosomal_L7/12.
IPR014719. Ribosomal_L7/12_C/ClpS-like.
IPR013823. Ribosomal_L7/L12_C.
IPR008932. Ribosomal_L7/L12_oligo.
[Graphical view ]
PANTHERi PTHR11809. PTHR11809. 1 hit.
Pfami PF00542. Ribosomal_L12. 1 hit.
[Graphical view ]
ProDomi PD001326. Ribosomal_L7/L12_C. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF48300. SSF48300. 1 hit.
SSF54736. SSF54736. 1 hit.
TIGRFAMsi TIGR00855. L12. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Ribosomal protein L12 and L10 from Thermus thermophilus, dissertation."
    Huang Y., Sprinzl M.
    Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  3. "Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus."
    Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T.
    Biol. Chem. 381:1079-1087(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-30.
  4. "Heptameric (L12)6/L10 rather than canonical pentameric complexes are found by tandem MS of intact ribosomes from thermophilic bacteria."
    Ilag L.L., Videler H., McKay A.R., Sobott F., Fucini P., Nierhaus K.H., Robinson C.V.
    Proc. Natl. Acad. Sci. U.S.A. 102:8192-8197(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, STOICHIOMETRY, PHOSPHORYLATION, MASS SPECTROMETRY.
  5. "Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry."
    Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.
    Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: MASS SPECTROMETRY.
  6. "The path of messenger RNA through the ribosome."
    Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F.
    Cell 106:233-241(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
  7. Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME.

Entry informationi

Entry nameiRL7_THET8
AccessioniPrimary (citable) accession number: Q8VVE2
Secondary accession number(s): Q5SLT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 87 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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