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Q8VVE2

- RL7_THET8

UniProt

Q8VVE2 - RL7_THET8

Protein

50S ribosomal protein L7/L12

Gene

rplL

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation. It extends beyond the surface of the 70S ribosome. The stalk is preferentially stabilized in 70S versus 50S crystals.

    GO - Molecular functioni

    1. structural constituent of ribosome Source: InterPro

    GO - Biological processi

    1. translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-219-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    50S ribosomal protein L7/L12UniRule annotation
    Gene namesi
    Name:rplLUniRule annotation
    Ordered Locus Names:TTHA0210
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. ribosome Source: UniProtKB-KW

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 12512450S ribosomal protein L7/L12PRO_0000157597Add
    BLAST

    Post-translational modificationi

    The ion at m/z 13016 is probably phosphorylated; treatment with phosphatase in the presence of kinase inhibitors decreases it weight. This form is only detected on 70S ribosomes and not in isolated ribosomal stalk complexes.1 Publication
    The ion at m/z 12978 is suggested to be the N-acetylated form of the protein (L7). The ion at m/z 12964 is suggested to be consistent with dimethylation or formylation and is present in very small quantities in the sample.

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Homodimer. Part of the 50S ribosomal subunit; present in 6 copies per ribosome. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which 3 L12 dimers bind in a sequential fashion. Contacts protein L11.1 Publication

    Protein-protein interaction databases

    STRINGi300852.TTHA0210.

    Structurei

    Secondary structure

    1
    125
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni2 – 76
    Helixi9 – 135
    Turni14 – 163
    Helixi17 – 226
    Turni25 – 273

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GIYX-ray5.50I/J32-125[»]
    1YL3X-ray5.50I/J32-125[»]
    2J01X-ray2.80L/M2-125[»]
    2J03X-ray2.80L/M2-125[»]
    3I8IX-ray3.10I/J1-125[»]
    ProteinModelPortaliQ8VVE2.
    SMRiQ8VVE2. Positions 3-125.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8VVE2.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L7/L12P family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0222.
    HOGENOMiHOG000248813.
    KOiK02935.
    OMAiEFISNMT.
    OrthoDBiEOG6WMJ69.
    PhylomeDBiQ8VVE2.

    Family and domain databases

    Gene3Di3.30.1390.10. 1 hit.
    HAMAPiMF_00368. Ribosomal_L7_L12.
    InterProiIPR000206. Ribosomal_L7/12.
    IPR014719. Ribosomal_L7/12_C/ClpS-like.
    IPR013823. Ribosomal_L7/L12_C.
    IPR008932. Ribosomal_L7/L12_oligo.
    [Graphical view]
    PANTHERiPTHR11809. PTHR11809. 1 hit.
    PfamiPF00542. Ribosomal_L12. 1 hit.
    [Graphical view]
    ProDomiPD001326. Ribosomal_L7/L12_C. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF48300. SSF48300. 1 hit.
    SSF54736. SSF54736. 1 hit.
    TIGRFAMsiTIGR00855. L12. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8VVE2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALDIERIKE ELSQATVLEL KQLIDALKEA WGVTAAAPVA VAAAPAAGAA    50
    AAPAEEKTEF DVILKEAGAK KLEVIKELRA ITGLGLKEAK DLAEKGGPVK 100
    EGVSKQEAEE IKKKLEAVGA VVELK 125
    Length:125
    Mass (Da):13,067
    Last modified:January 23, 2007 - v4
    Checksum:i3919CBF76605AC87
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti77 – 782EL → DV in CAD11987. 1 PublicationCurated

    Mass spectrometryi

    Molecular mass is 96075±13 Da from positions 2 - 125. Determined by ESI. Isolated L10(L12)6.1 Publication
    Molecular mass is 77670±9 Da from positions 2 - 125. Determined by ESI. Isolated (L12)6.1 Publication
    Molecular mass is 13016±1.9 Da from positions 2 - 125. Determined by ESI. Probably phosphorylated, more strongly associated with the 50S ribosomal subunit.1 Publication
    Molecular mass is 12936±0.5 Da from positions 2 - 125. Determined by ESI. Not phosphorylated.1 Publication
    Molecular mass is 12937 Da from positions 2 - 125. Determined by MALDI. 1 Publication
    Molecular mass is 12964 Da from positions 2 - 125. Determined by MALDI. May be N-acetylated.1 Publication
    Molecular mass is 12978 Da from positions 2 - 125. Determined by MALDI. May be dimethylated or formylated.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ419825 Genomic DNA. Translation: CAD11987.1.
    AP008226 Genomic DNA. Translation: BAD70033.1.
    RefSeqiYP_143476.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD70033; BAD70033; BAD70033.
    GeneIDi3168576.
    KEGGittj:TTHA0210.
    PATRICi23955361. VBITheThe93045_0208.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ419825 Genomic DNA. Translation: CAD11987.1 .
    AP008226 Genomic DNA. Translation: BAD70033.1 .
    RefSeqi YP_143476.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GIY X-ray 5.50 I/J 32-125 [» ]
    1YL3 X-ray 5.50 I/J 32-125 [» ]
    2J01 X-ray 2.80 L/M 2-125 [» ]
    2J03 X-ray 2.80 L/M 2-125 [» ]
    3I8I X-ray 3.10 I/J 1-125 [» ]
    ProteinModelPortali Q8VVE2.
    SMRi Q8VVE2. Positions 3-125.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 300852.TTHA0210.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD70033 ; BAD70033 ; BAD70033 .
    GeneIDi 3168576.
    KEGGi ttj:TTHA0210.
    PATRICi 23955361. VBITheThe93045_0208.

    Phylogenomic databases

    eggNOGi COG0222.
    HOGENOMi HOG000248813.
    KOi K02935.
    OMAi EFISNMT.
    OrthoDBi EOG6WMJ69.
    PhylomeDBi Q8VVE2.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-219-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q8VVE2.

    Family and domain databases

    Gene3Di 3.30.1390.10. 1 hit.
    HAMAPi MF_00368. Ribosomal_L7_L12.
    InterProi IPR000206. Ribosomal_L7/12.
    IPR014719. Ribosomal_L7/12_C/ClpS-like.
    IPR013823. Ribosomal_L7/L12_C.
    IPR008932. Ribosomal_L7/L12_oligo.
    [Graphical view ]
    PANTHERi PTHR11809. PTHR11809. 1 hit.
    Pfami PF00542. Ribosomal_L12. 1 hit.
    [Graphical view ]
    ProDomi PD001326. Ribosomal_L7/L12_C. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF48300. SSF48300. 1 hit.
    SSF54736. SSF54736. 1 hit.
    TIGRFAMsi TIGR00855. L12. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Ribosomal protein L12 and L10 from Thermus thermophilus, dissertation."
      Huang Y., Sprinzl M.
      Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.
    3. "Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus."
      Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T.
      Biol. Chem. 381:1079-1087(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-30.
    4. "Heptameric (L12)6/L10 rather than canonical pentameric complexes are found by tandem MS of intact ribosomes from thermophilic bacteria."
      Ilag L.L., Videler H., McKay A.R., Sobott F., Fucini P., Nierhaus K.H., Robinson C.V.
      Proc. Natl. Acad. Sci. U.S.A. 102:8192-8197(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, STOICHIOMETRY, PHOSPHORYLATION, MASS SPECTROMETRY.
    5. "Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry."
      Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.
      Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: MASS SPECTROMETRY.
    6. "The path of messenger RNA through the ribosome."
      Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F.
      Cell 106:233-241(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
    7. Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME.

    Entry informationi

    Entry nameiRL7_THET8
    AccessioniPrimary (citable) accession number: Q8VVE2
    Secondary accession number(s): Q5SLT3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 28, 2003
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 88 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3