Reviewed, UniProtKB/Swiss-Prot Q8VVE2 (RL7_THET8)

Names and origin

Protein names

Recommended name:
    50S ribosomal protein L7/L12

Gene names

Name:	rplL
Ordered Locus Names:	TTHA0210

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Complete proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	125 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	One of the L7 dimers in conjuction with L11 and its bound segment of 23S rRNA forms what is known as the L7/L12 stalk, which extends beyond the surface of the 70S ribosome. The stalk is preferentially stabilized in 70S versus 50S crystals. HAMAP MF_00368 Seems to be the binding site for several of the factors involved in protein synthesis and appears to be essential for accurate translation By similarity.
Subunit structure	Part of the 50S ribosomal subunit. Homodimer. Contacts protein L11. HAMAP MF_00368
Post-translational modification	The ion at m/z 12978 is suggested to be the N-acetylated form of the protein (L7). The ion at m/z 12964 is suggested to be consistent with dimethylation or formylation and is present in very small quantities in the sample. HAMAP MF_00368
Sequence similarities	Belongs to the ribosomal protein L12P family.
Mass spectrometry	Molecular mass is 12937 Da from positions 2 - 125. Determined by MALDI. Ref.4 Molecular mass is 12964 Da from positions 2 - 125. Determined by MALDI. May be N-acetylated. Ref.4 Molecular mass is 12978 Da from positions 2 - 125. Determined by MALDI. May be dimethylated or formylated. Ref.4

Ontologies

Keywords
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 125	124	50S ribosomal protein L7/L12 HAMAP MF_00368		PRO_0000157597
Experimental info
	Sequence conflict	77 – 78	2	EL → DV in CAD11987. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8VVE2-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 3919CBF76605AC87	FASTA	125	13,067
	10 20 30 40 50 60 MALDIERIKE ELSQATVLEL KQLIDALKEA WGVTAAAPVA VAAAPAAGAA AAPAEEKTEF 70 80 90 100 110 120 DVILKEAGAK KLEVIKELRA ITGLGLKEAK DLAEKGGPVK EGVSKQEAEE IKKKLEAVGA VVELK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ribosomal protein L12 and L10 from Thermus thermophilus, dissertation." Huang Y., Sprinzl M. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus." Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T. Biol. Chem. 381:1079-1087(2000) [PubMed: 11154066] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-30.
[4]	"Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry." Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A. Proteomics 5:4818-4831(2005) [PubMed: 16287167] [Abstract] Cited for: MASS SPECTROMETRY.
[5]	"The path of messenger RNA through the ribosome." Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F. Cell 106:233-241(2001) [PubMed: 11511350] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
[6]	"Crystal structure of the ribosome at 5.5 A resolution." Yusupov M.M., Yusupova G.Z., Baucom A., Lieberman K., Earnest T.N., Cate J.H.D., Noller H.F. Science 292:883-896(2001) [PubMed: 11283358] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AJ419825 Genomic DNA. Translation: CAD11987.1.
AP008226 Genomic DNA. Translation: BAD70033.1.

RefSeq

YP_143476.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GIY	X-ray	5.50	I/J	-	[»]
1YL3	X-ray	5.50	I/J	32-125	[»]
2J01	X-ray	2.80	L/M	2-124	[»]
2J03	X-ray	2.80	L/M	2-124	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q8VVE2.

Genome annotation databases

GeneID

3168576.

GenomeReviews

Gene locus TTHA0210 in contig AP008226_GR.

KEGG

ttj:TTHA0210.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

Q8VVE2.

OMA

KINVIKA.

Enzyme and pathway databases

BioCyc

TTHE300852:TTHA0210-MON.

Family and domain databases

HAMAP

MF_00368.
[Tree]

InterPro

IPR000206. Ribosomal_L7/12.
IPR014719. Ribosomal_L7/12_C/ClpS-like.
IPR013823. Ribosomal_L7/L12_C.
[Graphical view]

Gene3D

G3DSA:3.30.1390.10. Ribosomal_L7/12_C/ClpS-like. 1 hit.

Pfam

PF00542. Ribosomal_L12. 1 hit.
[Graphical view]

ProDom

PD001326. Ribosomal_L12. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00855. L12. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RL7_THET8

Accession

Primary (citable) accession number: Q8VVE2
Secondary accession number(s): Q5SLT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 55 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)