Q8VVE2 (RL7_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L7/L12

Gene names

Name:	rplL
Ordered Locus Names:	TTHA0210

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	125 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	One of the L7 dimers in conjuction with L11 and its bound segment of 23S rRNA forms what is known as the L7/L12 stalk, which extends beyond the surface of the 70S ribosome. The stalk is preferentially stabilized in 70S versus 50S crystals. HAMAP-Rule MF_00368 Seems to be the binding site for several of the factors involved in protein synthesis and appears to be essential for accurate translation By similarity. HAMAP-Rule MF_00368
Subunit structure	Part of the 50S ribosomal subunit. Homodimer. Contacts protein L11.
Post-translational modification	The ion at m/z 12978 is suggested to be the N-acetylated form of the protein (L7). The ion at m/z 12964 is suggested to be consistent with dimethylation or formylation and is present in very small quantities in the sample. HAMAP-Rule MF_00368
Sequence similarities	Belongs to the ribosomal protein L7/L12P family.
Mass spectrometry	Molecular mass is 12937 Da from positions 2 - 125. Determined by MALDI. Ref.4 Molecular mass is 12964 Da from positions 2 - 125. Determined by MALDI. May be N-acetylated. Ref.4 Molecular mass is 12978 Da from positions 2 - 125. Determined by MALDI. May be dimethylated or formylated. Ref.4

Ontologies

Keywords
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3

Chain

2 – 125

124

50S ribosomal protein L7/L12 HAMAP-Rule MF_00368

PRO_0000157597

Experimental info

Sequence conflict

77 – 78

2

EL → DV in CAD11987. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

125

Helix Strand Turn

Details...

Turn

2 – 7

6

Helix

9 – 13

5

Turn

14 – 16

3

Helix

17 – 22

6

Turn

25 – 27

3

Sequences

	Sequence		Length	Mass (Da)
	Q8VVE2 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 3919CBF76605AC87	FASTA	125	13,067
	10 20 30 40 50 60 MALDIERIKE ELSQATVLEL KQLIDALKEA WGVTAAAPVA VAAAPAAGAA AAPAEEKTEF 70 80 90 100 110 120 DVILKEAGAK KLEVIKELRA ITGLGLKEAK DLAEKGGPVK EGVSKQEAEE IKKKLEAVGA VVELK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ribosomal protein L12 and L10 from Thermus thermophilus, dissertation." Huang Y., Sprinzl M. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
[3]	"Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus." Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T. Biol. Chem. 381:1079-1087(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-30.
[4]	"Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry." Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A. Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY.
[5]	"The path of messenger RNA through the ribosome." Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F. Cell 106:233-241(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
[6]	"Crystal structure of the ribosome at 5.5 A resolution." Yusupov M.M., Yusupova G.Z., Baucom A., Lieberman K., Earnest T.N., Cate J.H.D., Noller H.F. Science 292:883-896(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ419825 Genomic DNA. Translation: CAD11987.1.
AP008226 Genomic DNA. Translation: BAD70033.1.

RefSeq

YP_143476.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GIY	X-ray	5.50	I/J	32-125	[»]
1YL3	X-ray	5.50	I/J	32-125	[»]
2J01	X-ray	2.80	L/M	2-124	[»]
2J03	X-ray	2.80	L/M	2-124	[»]
3I8I	X-ray	3.10	I/J	1-125	[»]

ProteinModelPortal

Q8VVE2.

SMR

Q8VVE2. Positions 3-125.

ModBase

Search...

Protein-protein interaction databases

STRING

300852.TTHA0210.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAD70033; BAD70033; BAD70033.

GeneID

3168576.

KEGG

ttj:TTHA0210.

PATRIC

23955361. VBITheThe93045_0208.

Phylogenomic databases

eggNOG

COG0222.

HOGENOM

HOG000248813.

KO

K02935.

OMA

MADLKDF.

ProtClustDB

PRK00157.

Family and domain databases

Gene3D

3.30.1390.10. 1 hit.

HAMAP

MF_00368. Ribosomal_L7_L12.

InterPro

IPR000206. Ribosomal_L7/12.
IPR014719. Ribosomal_L7/12_C/ClpS-like.
IPR013823. Ribosomal_L7/L12_C.
IPR008932. Ribosomal_L7/L12_oligo.
[Graphical view]

Pfam

PF00542. Ribosomal_L12. 1 hit.
[Graphical view]

ProDom

PD001326. Ribosomal_L7/L12_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF48300. Ribosomal_L12/7. 1 hit.
SSF54736. Ribosomal_L7/12_C/ClpS-like. 1 hit.

TIGRFAMs

TIGR00855. L12. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q8VVE2.

Entry information

Entry name

RL7_THET8

Accession

Primary (citable) accession number: Q8VVE2
Secondary accession number(s): Q5SLT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 78 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program