Q8VUR8 (DXS2_KITGR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase 2
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase 2
Short name=DXP synthase 2
Short name=DXPS 2

Gene names

Name:

dxs2

Organism

Kitasatospora griseola (Streptomyces griseolosporeus)

Taxonomic identifier

2064 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Kitasatospora

Protein attributes

Sequence length	633 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 633	633	1-deoxy-D-xylulose-5-phosphate synthase 2 HAMAP MF_00315		PRO_0000189122

Sequences

	Sequence		Length	Mass (Da)
	Q8VUR8 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: E521138A3751FE86	FASTA	633	67,639
	10 20 30 40 50 60 MALLTRIRGP RDLDRLTPAQ LAELAEEIRA FLVEEVSKTG GHLGPNLGVV ELTLAMHRVF 70 80 90 100 110 120 DSPRDRILFD TGHQSYVHKL LTGRQDFSRL KMKGGLSGYP SRAESEHDVI ENSHASTVLG 130 140 150 160 170 180 YADGLAKANK IQGHKDRPVV AVIGDGALTG GMAWEALNNI ADSQDLPIVI VVNDNERSYS 190 200 210 220 230 240 PTIGGLANHL ATLRTTQGYE RFLSWGKDAL QRTPVVGQAM FDTLHGAKKG LKDFIAPQGM 250 260 270 280 290 300 FEDLGLKYLG PIDGHDLQAL ESAFTKARNF GGPVIVHCIT EKGRGYHAAE NNDEDRFHAV 310 320 330 340 350 360 GVIHPDTGLP VKTSGKDWTS VFGEEMVALG RERRDLVAIT AAMLHPVGLA PFAKAYPERI 370 380 390 400 410 420 FDVGIAEQHA AVCAAGLATN GLHPVVAVYA TFLNRAFDQV LMDVALHKLG VTFVLDRAGV 430 440 450 460 470 480 TGTDGASHNG MWDMSILQVV PGLRLAAPRD AEQVRLQLRE AVEVADAPTV VRYSKGNVGP 490 500 510 520 530 540 AVPAVGTVGG MDVLRRPEAD SADEAADVLI VSIGAMAPTC LEAAELLAQQ GISSTVVDPR 550 560 570 580 590 600 WVKPVDAALP GLAAQHRLVV TVEDNGRAGG VGSAIAQALR DADVDVPLRD FGIPQEFLDH 610 620 630 ASRGEILDEI GLTAPAIAKK IEALVSTRSF ARS

References

[1]

"Growth-phase dependent expression of the mevalonate pathway in a terpenoid antibiotic-producing Streptomyces strain." Hamano Y., Dairi T., Yamamoto M., Kuzuyama T., Itoh N., Seto H. Biosci. Biotechnol. Biochem. 66:808-819(2002) [PubMed: 12036054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MF730-N6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB064999 Genomic DNA. Translation: BAB83664.1.
3D structure databases
ProteinModelPortal	Q8VUR8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR011766. TPP_enzyme-bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS2_KITGR

Accession

Primary (citable) accession number: Q8VUR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	March 1, 2002
Last modified:	September 21, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program