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Protein

Phosphothreonine lyase OspF

Gene

ospF

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the removal of the phosphate group from the phosphothreonine in the mitogen-activated protein kinases such as MAPK2/ERK2, MAPK3/ERK1, MAPK8 and MAPK14 in an irreversible reaction, thus preventing the downstream phosphorylation of histone H3. This epigenetic modification results in inhibition of the transcription of a specific subset of proinflammatory genes, and ultimately to a reduced immune response against the invading pathogen. The diminished immune response enhances the bacterium's ability to disseminate and multiply within the host.3 Publications

Enzyme regulationi

Inhibited by the tyrosine phosphatase inhibitor vanadate.1 Publication

Kineticsi

  1. KM=204 nM for phosphorylated MAPK2 (at pH 8 and 30 degrees Celsius)2 Publications
  1. Vmax=9.89 pM/sec/µg enzyme with phosphorylated MAPK2 as substrate (at pH 8 and 30 degrees Celsius)2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei104Proton donorBy similarity1
Active sitei134Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processVirulence

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphothreonine lyase OspF (EC:4.2.3.-)
Alternative name(s):
Effector protein OspF
Gene namesi
Name:ospF
Synonyms:mkaD
Ordered Locus Names:CP0010
ORF Names:pWR501_0013, SFLP011
Encoded oniPlasmid pWR1008 Publications
Plasmid pCP3012 Publications
Plasmid pSF51 Publication
Plasmid pINV_F6_M13821 Publication
Plasmid pSf2b_2511 Publication
Plasmid pSf1a_5711 Publication
Plasmid pSfx_5801 Publication
Plasmid pSf6_5791 Publication
Plasmid pSf3_5751 Publication
Plasmid pSf4a_5761 Publication
Plasmid pSf4b_5771 Publication
Plasmid pSf5_2461 Publication
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000001006 Componenti: Plasmid pCP301

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi101D → A: No change in Erk2 phosphothreonine lyase activity. 1 Publication1
Mutagenesisi102K → R: Loss of Erk2 phosphothreonine lyase activity. No effect in binding ability to erk. Deficient in down-regulating MAPK signaling. 1 Publication1
Mutagenesisi104H → A: Loss of Erk2 phosphothreonine lyase activity. No effect in binding ability to erk. Deficient in down-regulating MAPK signaling. 1 Publication1
Mutagenesisi127D → A: No change in Erk2 phosphothreonine lyase activity. 1 Publication1
Mutagenesisi131D → A: No change in Erk2 phosphothreonine lyase activity. 1 Publication1
Mutagenesisi134K → A: Loss of Erk2 phosphothreonine lyase activity. No effect in binding ability to erk. Deficient in down-regulating MAPK signaling. 1 Publication1
Mutagenesisi137D → A: No change in Erk2 phosphothreonine lyase activity. 1 Publication1
Mutagenesisi160D → A: No change in Erk2 phosphothreonine lyase activity. 1 Publication1
Mutagenesisi175R → A: No change in Erk2 phosphothreonine lyase activity. 1 Publication1
Mutagenesisi218R → A: No change in Erk2 phosphothreonine lyase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002993541 – 239Phosphothreonine lyase OspFAdd BLAST239

Proteomic databases

PaxDbiQ8VSP9.
PRIDEiQ8VSP9.

Expressioni

Inductioni

Transcriptionally activated by MxiE in the intracellular environment of the host, in association with IpgC, under conditions of deregulated or active secretion. Expressed in a VirB-dependent but MxiE-independent way under conditions of non-secretion.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RB1P064002EBI-6506625,EBI-491274From Homo sapiens.

Protein-protein interaction databases

IntActiQ8VSP9. 1 interactor.

Structurei

Secondary structure

1239
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi32 – 43Combined sources12
Helixi60 – 65Combined sources6
Beta strandi67 – 71Combined sources5
Beta strandi74 – 77Combined sources4
Beta strandi82 – 91Combined sources10
Beta strandi98 – 105Combined sources8
Helixi109 – 111Combined sources3
Helixi112 – 123Combined sources12
Beta strandi131 – 136Combined sources6
Turni138 – 140Combined sources3
Beta strandi153 – 158Combined sources6
Helixi168 – 187Combined sources20
Beta strandi206 – 214Combined sources9
Helixi232 – 238Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I0UX-ray2.70A/B23-239[»]
ProteinModelPortaliQ8VSP9.
SMRiQ8VSP9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8VSP9.

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphothreonine lyase family.Curated

Phylogenomic databases

eggNOGiENOG41086SQ. Bacteria.
ENOG410ZMKR. LUCA.
HOGENOMiHOG000028458.
KOiK13450.
OMAiFTLYVKP.

Family and domain databases

InterProiView protein in InterPro
IPR003519. OspF/SpvC.
PfamiView protein in Pfam
PF03536. VRP3. 1 hit.
PRINTSiPR01342. SALVRPPROT.

Sequencei

Sequence statusi: Complete.

Q8VSP9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIKKPCLKL NLDSLNVVRS EIPQMLSANE RLKNNFNILY NQIRQYPAYY
60 70 80 90 100
FKVASNVPTY SDICQSFSVM YQGFQIVNHS GDVFIHACRE NPQSKGDFVG
110 120 130 140 150
DKFHISIARE QVPLAFQILS GLLFSEDSPI DKWKITDMNR VSQQSRVGIG
160 170 180 190 200
AQFTLYVKSD QECSQYSALL LHKIRQFIMC LESNLLRSKI APGEYPASDV
210 220 230
RPEDWKYVSY RNELRSDRDG SERQEQMLRE EPFYRLMIE
Length:239
Mass (Da):27,828
Last modified:March 1, 2002 - v1
Checksum:i75F6EA2D6FFD002A
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti19R → K in plasmid pWR100 and plasmid pSF5. 1
Natural varianti66S → F in plasmid pWR100, plasmid pSF5, plasmid pINV_F6_M1382, plasmid pSf6_579, plasmid pSf3_575, plasmid pSf4a_576, plasmid pSf4b_577 and plasmid pSf5_246. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL391753 Genomic DNA. Translation: CAC05773.1.
AF348706 Genomic DNA. Translation: AAK18324.1.
AY206429 Genomic DNA. Translation: AAP78969.1.
AY879342 Genomic DNA. Translation: AAW64770.1.
AF386526 Genomic DNA. Translation: AAL72315.1.
DQ362850 Genomic DNA. Translation: ABD37257.1.
DQ362864 Genomic DNA. Translation: ABD37271.1.
DQ362865 Genomic DNA. Translation: ABD37272.1.
DQ362866 Genomic DNA. Translation: ABD37273.1.
DQ362867 Genomic DNA. Translation: ABD37274.1.
DQ362868 Genomic DNA. Translation: ABD37275.1.
DQ362869 Genomic DNA. Translation: ABD37276.1.
DQ362870 Genomic DNA. Translation: ABD37277.1.
RefSeqiNP_858143.1. NC_004851.1.
WP_001121867.1. NZ_MSKF02000260.1.
WP_010921598.1. NC_019197.1.
YP_006960239.1. NC_019197.1.

Genome annotation databases

EnsemblBacteriaiAAL72315; AAL72315; SF_p0010.
GeneIDi1238011.
13917039.
KEGGisfl:CP0010.

Similar proteinsi

Entry informationi

Entry nameiOSPF_SHIFL
AccessioniPrimary (citable) accession number: Q8VSP9
Secondary accession number(s): Q2ET10
, Q2ET14, Q6XW10, Q7BEG0, Q99Q87
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 1, 2002
Last modified: July 5, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families