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Protein

K(+)-insensitive pyrophosphate-energized proton pump

Gene

hppA

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force.UniRule annotation

Catalytic activityi

Diphosphate + H2O = 2 phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei178 – 1781Important for ion transportBy similarity
Binding sitei186 – 1861SubstrateBy similarity
Metal bindingi189 – 1891Magnesium 1By similarity
Metal bindingi193 – 1931Magnesium 1By similarity
Metal bindingi216 – 2161Magnesium 2By similarity
Metal bindingi219 – 2191Magnesium 2By similarity
Sitei223 – 2231Important for ion transportBy similarity
Sitei230 – 2301Important for ion transportBy similarity
Metal bindingi437 – 4371Magnesium 2By similarity
Sitei467 – 4671Important for potassium independenceBy similarity
Metal bindingi646 – 6461Calcium or magnesiumBy similarity
Metal bindingi678 – 6781Calcium or magnesiumBy similarity
Metal bindingi682 – 6821Calcium or magnesiumBy similarity
Binding sitei685 – 6851SubstrateBy similarity
Sitei686 – 6861Important for ion transportBy similarity
Sitei697 – 6971Important for ion transportBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-1244-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
K(+)-insensitive pyrophosphate-energized proton pumpUniRule annotation (EC:3.6.1.1UniRule annotation)
Alternative name(s):
Membrane-bound proton-translocating pyrophosphataseUniRule annotation
Pyrophosphate-energized inorganic pyrophosphataseUniRule annotation
Short name:
H(+)-PPaseUniRule annotation
Gene namesi
Name:hppAUniRule annotation
Ordered Locus Names:R01218
ORF Names:SMc01780
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalUniRule annotationAdd
BLAST
Transmembranei48 – 7023HelicalUniRule annotationAdd
BLAST
Transmembranei83 – 10321HelicalUniRule annotationAdd
BLAST
Transmembranei126 – 14621HelicalUniRule annotationAdd
BLAST
Transmembranei164 – 18421HelicalUniRule annotationAdd
BLAST
Transmembranei239 – 25921HelicalUniRule annotationAdd
BLAST
Transmembranei261 – 28121HelicalUniRule annotationAdd
BLAST
Transmembranei296 – 31621HelicalUniRule annotationAdd
BLAST
Transmembranei331 – 35121HelicalUniRule annotationAdd
BLAST
Transmembranei382 – 40221HelicalUniRule annotationAdd
BLAST
Transmembranei409 – 42921HelicalUniRule annotationAdd
BLAST
Transmembranei468 – 48821HelicalUniRule annotationAdd
BLAST
Transmembranei520 – 54021HelicalUniRule annotationAdd
BLAST
Transmembranei589 – 60921HelicalUniRule annotationAdd
BLAST
Transmembranei616 – 63621HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 711711K(+)-insensitive pyrophosphate-energized proton pumpPRO_0000217027Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi266834.SMc01780.

Structurei

3D structure databases

ProteinModelPortaliQ8VRZ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-insensitive subfamily. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EAY. Bacteria.
COG3808. LUCA.
HOGENOMiHOG000007098.
KOiK15987.
OMAiNVMPALY.
OrthoDBiEOG6TXQSF.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8VRZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTILLGVIAC GLLSVVYAIW ATKSVLAADQ GNARMQEIAG FIREGAQAYL
60 70 80 90 100
TRQYTTIAIV GVVVFIAAWL LLSGAAAIGF LIGAVLSGAA GFIGMHVSVR
110 120 130 140 150
ANVRTAQAAS VSLASGLDIA FKSGAITGML VAGLALLGVS VYYLILTAGL
160 170 180 190 200
GHEPAAREVI DALVALGFGA SLISIFARLG GGIFTKGADV GGDLVGKVEA
210 220 230 240 250
GIPEDDPRNP ATIADNVGDN VGDCAGMAAD LFETYAVSVV ATMVLASIFF
260 270 280 290 300
AGAPVLATVM TYPLAICAAC IITSIIGTFF VKLGANASIM GALYRGLIVT
310 320 330 340 350
GALSILGLGA ATSLTIGWGS IGTVAGMDIT GWNLFLCGII GLIVTALIVV
360 370 380 390 400
ITEYYTGTNK RPVNSIAQAS VTGHGTNVIQ GLAVSLESTA LPAIVIVGGI
410 420 430 440 450
IATYQFAGLF GTAIAVTAML GLAGMIVALD AFGPVTDNAG GIAEMSHLPP
460 470 480 490 500
EVRKSTDALD AVGNTTKAVT KGYAIGSAGL GALVLFAAYS NDLAYFAANG
510 520 530 540 550
DKHPYFADVG TISFDLSNPY VVAGLIFGGL IPYLFGGIAM TAVGRAGSAV
560 570 580 590 600
VEEVRRQFKE KPGIMEGKDR PDYGRAVDML TKAAIREMII PSLLPVLAPI
610 620 630 640 650
VVYFGVLLIS GSKASAFAAL GASLLGVIVN GLFVAISMTS GGGAWDNAKK
660 670 680 690 700
SFEDGFVDRN GTRHMKGSEA HKASVTGDTV GDPYKDTAGP AVNPAIKITN
710
IVALLLLAVL A
Length:711
Mass (Da):72,257
Last modified:July 25, 2003 - v2
Checksum:i55AAD7CF3242A681
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 381I → V in AAL69329 (Ref. 3) Curated
Sequence conflicti116 – 1172GL → DI in AAL69329 (Ref. 3) Curated
Sequence conflicti144 – 15815LILTA…PAARE → FVLIVWLGYAPADRA in AAL69329 (Ref. 3) CuratedAdd
BLAST
Sequence conflicti162 – 1621A → S in AAL69329 (Ref. 3) Curated
Sequence conflicti174 – 1741S → A in AAL69329 (Ref. 3) Curated
Sequence conflicti177 – 1771A → V in AAL69329 (Ref. 3) Curated
Sequence conflicti201 – 2033GIP → CIR in AAL69329 (Ref. 3) Curated
Sequence conflicti208 – 2092RN → CI in AAL69329 (Ref. 3) Curated
Sequence conflicti215 – 2151D → N in AAL69329 (Ref. 3) Curated
Sequence conflicti219 – 2191D → H in AAL69329 (Ref. 3) Curated
Sequence conflicti222 – 2232GD → CG in AAL69329 (Ref. 3) Curated
Sequence conflicti226 – 2261G → A in AAL69329 (Ref. 3) Curated
Sequence conflicti238 – 2381S → T in AAL69329 (Ref. 3) Curated
Sequence conflicti456 – 4561T → N in AAL69329 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591688 Genomic DNA. Translation: CAC45797.1.
AF417513 Genomic DNA. Translation: AAL69329.1.
RefSeqiNP_385324.1. NC_003047.1.
WP_010969115.1. NC_003047.1.

Genome annotation databases

EnsemblBacteriaiCAC45797; CAC45797; SMc01780.
GeneIDi1232862.
KEGGisme:SMc01780.
PATRICi23631739. VBISinMel96828_2630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591688 Genomic DNA. Translation: CAC45797.1.
AF417513 Genomic DNA. Translation: AAL69329.1.
RefSeqiNP_385324.1. NC_003047.1.
WP_010969115.1. NC_003047.1.

3D structure databases

ProteinModelPortaliQ8VRZ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266834.SMc01780.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC45797; CAC45797; SMc01780.
GeneIDi1232862.
KEGGisme:SMc01780.
PATRICi23631739. VBISinMel96828_2630.

Phylogenomic databases

eggNOGiENOG4105EAY. Bacteria.
COG3808. LUCA.
HOGENOMiHOG000007098.
KOiK15987.
OMAiNVMPALY.
OrthoDBiEOG6TXQSF.

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-1244-MONOMER.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.
  3. "High prevalence of the H+ proton-pumping inorganic pyrophosphatase gene in alpha proteobacteria and evidence of lateral transfer in its phylogeny."
    Jumas-Bilak E., Michaux-Charachon S., Teyssier C.
    Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 35-698.
    Strain: RCR2011 / SU47.

Entry informationi

Entry nameiHPPA_RHIME
AccessioniPrimary (citable) accession number: Q8VRZ3
Secondary accession number(s): Q92QT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: July 25, 2003
Last modified: November 11, 2015
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.