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Q8VRZ3 (HPPA_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
K(+)-insensitive pyrophosphate-energized proton pump
EC=3.6.1.1
Alternative name(s):
Membrane-bound proton-translocating pyrophosphatase
Pyrophosphate-energized inorganic pyrophosphatase
Short name=H(+)-PPase
Gene names
Name:hppA
Ordered Locus Names:R01218
ORF Names:SMc01780
OrganismRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]
Taxonomic identifier266834 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length711 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force By similarity. HAMAP MF_01129

Catalytic activity

Diphosphate + H2O = 2 phosphate. HAMAP MF_01129

Cofactor

Magnesium By similarity. HAMAP MF_01129

Subunit structure

Homodimer By similarity. HAMAP MF_01129

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_01129.

Sequence similarities

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-insensitive subfamily. [View classification]

Ontologies

Keywords
   Biological processHydrogen ion transport
Ion transport
Transport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandMagnesium
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processproton transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionhydrogen-translocating pyrophosphatase activity

Inferred from electronic annotation. Source: InterPro

inorganic diphosphatase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 711711K(+)-insensitive pyrophosphate-energized proton pump HAMAP MF_01129
PRO_0000217027

Regions

Transmembrane1 – 2121Helical; Potential
Transmembrane48 – 7023Helical; Potential
Transmembrane83 – 10321Helical; Potential
Transmembrane126 – 14621Helical; Potential
Transmembrane164 – 18421Helical; Potential
Transmembrane239 – 25921Helical; Potential
Transmembrane261 – 28121Helical; Potential
Transmembrane296 – 31621Helical; Potential
Transmembrane331 – 35121Helical; Potential
Transmembrane382 – 40221Helical; Potential
Transmembrane409 – 42921Helical; Potential
Transmembrane468 – 48821Helical; Potential
Transmembrane520 – 54021Helical; Potential
Transmembrane589 – 60921Helical; Potential
Transmembrane616 – 63621Helical; Potential

Sites

Site4671Determinant of potassium independence By similarity

Experimental info

Sequence conflict381I → V in AAL69329. Ref.3
Sequence conflict116 – 1172GL → DI in AAL69329. Ref.3
Sequence conflict144 – 15815LILTA…PAARE → FVLIVWLGYAPADRA in AAL69329. Ref.3
Sequence conflict1621A → S in AAL69329. Ref.3
Sequence conflict1741S → A in AAL69329. Ref.3
Sequence conflict1771A → V in AAL69329. Ref.3
Sequence conflict201 – 2033GIP → CIR in AAL69329. Ref.3
Sequence conflict208 – 2092RN → CI in AAL69329. Ref.3
Sequence conflict2151D → N in AAL69329. Ref.3
Sequence conflict2191D → H in AAL69329. Ref.3
Sequence conflict222 – 2232GD → CG in AAL69329. Ref.3
Sequence conflict2261G → A in AAL69329. Ref.3
Sequence conflict2381S → T in AAL69329. Ref.3
Sequence conflict4561T → N in AAL69329. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8VRZ3 [UniParc].

Last modified July 25, 2003. Version 2.
Checksum: 55AAD7CF3242A681

FASTA71172,257
        10         20         30         40         50         60 
MTILLGVIAC GLLSVVYAIW ATKSVLAADQ GNARMQEIAG FIREGAQAYL TRQYTTIAIV 

        70         80         90        100        110        120 
GVVVFIAAWL LLSGAAAIGF LIGAVLSGAA GFIGMHVSVR ANVRTAQAAS VSLASGLDIA 

       130        140        150        160        170        180 
FKSGAITGML VAGLALLGVS VYYLILTAGL GHEPAAREVI DALVALGFGA SLISIFARLG 

       190        200        210        220        230        240 
GGIFTKGADV GGDLVGKVEA GIPEDDPRNP ATIADNVGDN VGDCAGMAAD LFETYAVSVV 

       250        260        270        280        290        300 
ATMVLASIFF AGAPVLATVM TYPLAICAAC IITSIIGTFF VKLGANASIM GALYRGLIVT 

       310        320        330        340        350        360 
GALSILGLGA ATSLTIGWGS IGTVAGMDIT GWNLFLCGII GLIVTALIVV ITEYYTGTNK 

       370        380        390        400        410        420 
RPVNSIAQAS VTGHGTNVIQ GLAVSLESTA LPAIVIVGGI IATYQFAGLF GTAIAVTAML 

       430        440        450        460        470        480 
GLAGMIVALD AFGPVTDNAG GIAEMSHLPP EVRKSTDALD AVGNTTKAVT KGYAIGSAGL 

       490        500        510        520        530        540 
GALVLFAAYS NDLAYFAANG DKHPYFADVG TISFDLSNPY VVAGLIFGGL IPYLFGGIAM 

       550        560        570        580        590        600 
TAVGRAGSAV VEEVRRQFKE KPGIMEGKDR PDYGRAVDML TKAAIREMII PSLLPVLAPI 

       610        620        630        640        650        660 
VVYFGVLLIS GSKASAFAAL GASLLGVIVN GLFVAISMTS GGGAWDNAKK SFEDGFVDRN 

       670        680        690        700        710 
GTRHMKGSEA HKASVTGDTV GDPYKDTAGP AVNPAIKITN IVALLLLAVL A

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021."
Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. expand/collapse author list , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F.
Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.
[2]"The composite genome of the legume symbiont Sinorhizobium meliloti."
Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. expand/collapse author list , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J.
Science 293:668-672(2001) [PubMed: 11474104] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.
[3]"High prevalence of the H+ proton-pumping inorganic pyrophosphatase gene in alpha proteobacteria and evidence of lateral transfer in its phylogeny."
Jumas-Bilak E., Michaux-Charachon S., Teyssier C.
Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 35-698.
Strain: RCR2011 / SU47.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL591688 Genomic DNA. Translation: CAC45797.1.
AF417513 Genomic DNA. Translation: AAL69329.1.
RefSeqNP_385324.1. NC_003047.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1232862.
GenomeReviewsGene locus R01218 in contig AL591688_GR.
KEGGsme:SMc01780.
NMPDRfig|266834.1.peg.2512.
PATRIC23631739. VBISinMel96828_2630.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG593668.
OMAAINRGFF.
ProtClustDBPRK00733.

Enzyme and pathway databases

BioCycSMEL266834:SMC01780-MONOMER.

Family and domain databases

HAMAPMF_01129. PPase-energized_pump.
[Tree]
InterProIPR004131. PPase-energised_H-pump.
[Graphical view]
KOK01507.
PfamPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsTIGR01104. V_PPase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHPPA_RHIME
AccessionPrimary (citable) accession number: Q8VRZ3
Secondary accession number(s): Q92QT7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: July 25, 2003
Last modified: January 25, 2012
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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