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Protein

K(+)-insensitive pyrophosphate-energized proton pump

Gene

hppA

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force.UniRule annotation

Catalytic activityi

Diphosphate + H2O = 2 phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei178Important for ion transportBy similarity1
Binding sitei186SubstrateBy similarity1
Metal bindingi189Magnesium 1By similarity1
Metal bindingi193Magnesium 1By similarity1
Metal bindingi216Magnesium 2By similarity1
Metal bindingi219Magnesium 2By similarity1
Sitei223Important for ion transportBy similarity1
Sitei230Important for ion transportBy similarity1
Metal bindingi437Magnesium 2By similarity1
Sitei467Important for potassium independenceBy similarity1
Metal bindingi646Calcium or magnesiumBy similarity1
Metal bindingi678Calcium or magnesiumBy similarity1
Metal bindingi682Calcium or magnesiumBy similarity1
Binding sitei685SubstrateBy similarity1
Sitei686Important for ion transportBy similarity1
Sitei697Important for ion transportBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
K(+)-insensitive pyrophosphate-energized proton pumpUniRule annotation (EC:3.6.1.1UniRule annotation)
Alternative name(s):
Membrane-bound proton-translocating pyrophosphataseUniRule annotation
Pyrophosphate-energized inorganic pyrophosphataseUniRule annotation
Short name:
H(+)-PPaseUniRule annotation
Gene namesi
Name:hppAUniRule annotation
Ordered Locus Names:R01218
ORF Names:SMc01780
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21HelicalUniRule annotationAdd BLAST21
Transmembranei48 – 70HelicalUniRule annotationAdd BLAST23
Transmembranei83 – 103HelicalUniRule annotationAdd BLAST21
Transmembranei126 – 146HelicalUniRule annotationAdd BLAST21
Transmembranei164 – 184HelicalUniRule annotationAdd BLAST21
Transmembranei239 – 259HelicalUniRule annotationAdd BLAST21
Transmembranei261 – 281HelicalUniRule annotationAdd BLAST21
Transmembranei296 – 316HelicalUniRule annotationAdd BLAST21
Transmembranei331 – 351HelicalUniRule annotationAdd BLAST21
Transmembranei382 – 402HelicalUniRule annotationAdd BLAST21
Transmembranei409 – 429HelicalUniRule annotationAdd BLAST21
Transmembranei468 – 488HelicalUniRule annotationAdd BLAST21
Transmembranei520 – 540HelicalUniRule annotationAdd BLAST21
Transmembranei589 – 609HelicalUniRule annotationAdd BLAST21
Transmembranei616 – 636HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002170271 – 711K(+)-insensitive pyrophosphate-energized proton pumpAdd BLAST711

Proteomic databases

PRIDEiQ8VRZ3.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi266834.SMc01780.

Structurei

3D structure databases

ProteinModelPortaliQ8VRZ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-insensitive subfamily. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EAY. Bacteria.
COG3808. LUCA.
HOGENOMiHOG000007098.
KOiK15987.
OMAiNVMPALY.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump. 1 hit.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8VRZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTILLGVIAC GLLSVVYAIW ATKSVLAADQ GNARMQEIAG FIREGAQAYL
60 70 80 90 100
TRQYTTIAIV GVVVFIAAWL LLSGAAAIGF LIGAVLSGAA GFIGMHVSVR
110 120 130 140 150
ANVRTAQAAS VSLASGLDIA FKSGAITGML VAGLALLGVS VYYLILTAGL
160 170 180 190 200
GHEPAAREVI DALVALGFGA SLISIFARLG GGIFTKGADV GGDLVGKVEA
210 220 230 240 250
GIPEDDPRNP ATIADNVGDN VGDCAGMAAD LFETYAVSVV ATMVLASIFF
260 270 280 290 300
AGAPVLATVM TYPLAICAAC IITSIIGTFF VKLGANASIM GALYRGLIVT
310 320 330 340 350
GALSILGLGA ATSLTIGWGS IGTVAGMDIT GWNLFLCGII GLIVTALIVV
360 370 380 390 400
ITEYYTGTNK RPVNSIAQAS VTGHGTNVIQ GLAVSLESTA LPAIVIVGGI
410 420 430 440 450
IATYQFAGLF GTAIAVTAML GLAGMIVALD AFGPVTDNAG GIAEMSHLPP
460 470 480 490 500
EVRKSTDALD AVGNTTKAVT KGYAIGSAGL GALVLFAAYS NDLAYFAANG
510 520 530 540 550
DKHPYFADVG TISFDLSNPY VVAGLIFGGL IPYLFGGIAM TAVGRAGSAV
560 570 580 590 600
VEEVRRQFKE KPGIMEGKDR PDYGRAVDML TKAAIREMII PSLLPVLAPI
610 620 630 640 650
VVYFGVLLIS GSKASAFAAL GASLLGVIVN GLFVAISMTS GGGAWDNAKK
660 670 680 690 700
SFEDGFVDRN GTRHMKGSEA HKASVTGDTV GDPYKDTAGP AVNPAIKITN
710
IVALLLLAVL A
Length:711
Mass (Da):72,257
Last modified:July 25, 2003 - v2
Checksum:i55AAD7CF3242A681
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38I → V in AAL69329 (Ref. 3) Curated1
Sequence conflicti116 – 117GL → DI in AAL69329 (Ref. 3) Curated2
Sequence conflicti144 – 158LILTA…PAARE → FVLIVWLGYAPADRA in AAL69329 (Ref. 3) CuratedAdd BLAST15
Sequence conflicti162A → S in AAL69329 (Ref. 3) Curated1
Sequence conflicti174S → A in AAL69329 (Ref. 3) Curated1
Sequence conflicti177A → V in AAL69329 (Ref. 3) Curated1
Sequence conflicti201 – 203GIP → CIR in AAL69329 (Ref. 3) Curated3
Sequence conflicti208 – 209RN → CI in AAL69329 (Ref. 3) Curated2
Sequence conflicti215D → N in AAL69329 (Ref. 3) Curated1
Sequence conflicti219D → H in AAL69329 (Ref. 3) Curated1
Sequence conflicti222 – 223GD → CG in AAL69329 (Ref. 3) Curated2
Sequence conflicti226G → A in AAL69329 (Ref. 3) Curated1
Sequence conflicti238S → T in AAL69329 (Ref. 3) Curated1
Sequence conflicti456T → N in AAL69329 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591688 Genomic DNA. Translation: CAC45797.1.
AF417513 Genomic DNA. Translation: AAL69329.1.
RefSeqiNP_385324.1. NC_003047.1.
WP_010969115.1. NC_003047.1.

Genome annotation databases

EnsemblBacteriaiCAC45797; CAC45797; SMc01780.
GeneIDi1232862.
KEGGisme:SMc01780.
PATRICi23631739. VBISinMel96828_2630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591688 Genomic DNA. Translation: CAC45797.1.
AF417513 Genomic DNA. Translation: AAL69329.1.
RefSeqiNP_385324.1. NC_003047.1.
WP_010969115.1. NC_003047.1.

3D structure databases

ProteinModelPortaliQ8VRZ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266834.SMc01780.

Proteomic databases

PRIDEiQ8VRZ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC45797; CAC45797; SMc01780.
GeneIDi1232862.
KEGGisme:SMc01780.
PATRICi23631739. VBISinMel96828_2630.

Phylogenomic databases

eggNOGiENOG4105EAY. Bacteria.
COG3808. LUCA.
HOGENOMiHOG000007098.
KOiK15987.
OMAiNVMPALY.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump. 1 hit.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHPPA_RHIME
AccessioniPrimary (citable) accession number: Q8VRZ3
Secondary accession number(s): Q92QT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: July 25, 2003
Last modified: November 2, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.