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Reviewed, UniProtKB/Swiss-Prot Q8VRZ2 (HPPA_OCHA4)

Last modified November 3, 2009. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase
Gene names
Name: hppA
Ordered Locus Names: Oant_2524
OrganismOchrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168) [Complete proteome] [HAMAP]
Taxonomic identifier439375 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Ochrobactrum

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Protein attributes

Sequence length718 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity.

Catalytic activity

Diphosphate + H2O = 2 phosphate. HAMAP MF_01130

Cofactor

Magnesium By similarity.

Subunit structure

Homodimer Potential.

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Sequence similarities

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 2 subfamily. [View classification]

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Ontologies

Keywords
   Biological processHydrogen ion transport
Ion transport
Transport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
   LigandMagnesium
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processproton transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from electronic annotation. Source: HAMAP

   Molecular functionhydrogen-translocating pyrophosphatase activity

Inferred from electronic annotation. Source: InterPro

inorganic diphosphatase activity

Inferred from electronic annotation. Source: HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 718718Pyrophosphate-energized proton pump HAMAP MF_01130
PRO_0000217023

Regions

Transmembrane6 – 2621 Potential
Transmembrane54 – 7623 Potential
Transmembrane81 – 10323 Potential
Transmembrane133 – 15321 Potential
Transmembrane168 – 18821 Potential
Transmembrane240 – 26021 Potential
Transmembrane265 – 28521 Potential
Transmembrane300 – 32021 Potential
Transmembrane335 – 35521 Potential
Transmembrane385 – 40521 Potential
Transmembrane413 – 43321 Potential
Transmembrane472 – 49221 Potential
Transmembrane524 – 54421 Potential
Transmembrane593 – 61321 Potential
Transmembrane620 – 64021 Potential
Transmembrane695 – 71521 Potential

Sites

Site4711Determinant of potassium independence By similarity

Experimental info

Sequence conflict2591I → V in AAL69330. Ref.2
Sequence conflict288 – 2903GVN → SVD in AAL69330. Ref.2
Sequence conflict3141I → V in AAL69330. Ref.2
Sequence conflict3201V → I in AAL69330. Ref.2
Sequence conflict3401L → I in AAL69330. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Q8VRZ2-1 [UniParc].

Last modified November 13, 2007. Version 2.
Checksum: 478B489906207833

FASTA71873,272
        10         20         30         40         50         60 
MQMGVAVLVL VIACGVVSVL FAIWAIRSVL AADQGTQRMQ EIAEAIREGA SAYLTRQYST 

        70         80         90        100        110        120 
IAIVGVVVFL AAWYLLSISA AIGFLIGAVL SGVTGFIGMH VSVRANVRTA QAASLSLAGG 

       130        140        150        160        170        180 
LELAFKSGAI TGLLVAGLAL LGVSVYYFIL TVWLGYSPSD RTVIDALVSL GFGASLISIF 

       190        200        210        220        230        240 
ARLGGGIFTK GADVGGDLVG KVEAGIPEDD PRNPATIADN VGDNVGDCAG MAADLFETYA 

       250        260        270        280        290        300 
VTVVATMVLG AIFFNGSDIL SSVMLYPLMI CGACVITSIV GTFFVKLGVN GSIMGALYKG 

       310        320        330        340        350        360 
LIATGLLSIV GLAIANTLTV GWGEIGTVAG KSITGTNLFL CGLIGLIVTG LIVVITEYYT 

       370        380        390        400        410        420 
GTNKRPVNSI AQASVTGHGT NVIQGLAVSL ESTALPAIVI VGGIISTYQL AGLFGTAIAV 

       430        440        450        460        470        480 
TAMLGIAGMI VALDAFGPVT DNAGGIAEMA GLDPEVRKAT DALDAVGNTT KAVTKGYAIG 

       490        500        510        520        530        540 
SAGLGALVLF AAYSNDLAYF AANGQTYPYF ADMGPVSFDL SNPYVVAGLI FGGLIPYLFG 

       550        560        570        580        590        600 
GMAMTAVGRA GGAVVQEVRR QFREKPGIMT GKERPDYARA VDLLTRAAIR EMIIPSLLPV 

       610        620        630        640        650        660 
LAPIVVYFGV LLISGSKAAA FAALGASLLG VIVNGLFVAI SMTSGGGAWD NAKKSFEDGF 

       670        680        690        700        710 
TDADGVKHLK GSEAHKASVT GDTVGDPYKD TAGPAVNPAI KITNIVALLL LAVLAHMS

« Hide

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References

« Hide 'large scale' references
[1]"Complete sequence of chromosome 1 of Ochrobactrum anthropi ATCC 49188."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Garcia E., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"High prevalence of the H+ proton-pumping inorganic pyrophosphatase gene in alpha proteobacteria and evidence of lateral transfer in its phylogeny."
Jumas-Bilak E., Michaux-Charachon S., Teyssier C.
Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 242-455.

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Cross-references

Sequence databases

CP000758 Genomic DNA. Translation: ABS15237.1.
AF417514 Genomic DNA. Translation: AAL69330.1.
RefSeqYP_001371066.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ8VRZ2.

Genome annotation databases

GeneID5380886.
GenomeReviewsGene locus Oant_2524 in contig CP000758_GR.
KEGGoan:Oant_2524.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAPYLFGGI.

Family and domain databases

HAMAPMF_01130.
[Tree]
InterProIPR004131. H_PPase.
[Graphical view]
PfamPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsTIGR01104. V_PPase. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameHPPA_OCHA4
AccessionPrimary (citable) accession number: Q8VRZ2
Secondary accession number(s): A6X1Y3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: November 13, 2007
Last modified: November 3, 2009
This is version 41 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents