Reviewed, UniProtKB/Swiss-Prot Q8VRZ1 (HPPA_ANAMA)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase

Gene names

Name:

hppA

Organism

Anaplasma marginale

Taxonomic identifier

770 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma

Protein attributes

Sequence length	141 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity.
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01130
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 2 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›141	›141	Pyrophosphate-energized proton pump HAMAP MF_01130		PRO_0000217009
Regions
	Transmembrane	12 – 34	23	Potential
	Transmembrane	44 – 66	23	Potential
	Transmembrane	109 – 131	23	Potential
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	141	1

Sequences

	Sequence		Length	Mass (Da)
	Q8VRZ1-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 6B684177277A6E1C	FASTA	141	14,012
	10 20 30 40 50 60 NGSIMGALYK GLIATGLLSI VGLGVANTLT VGWGEIGTVA GKSITGTNLF VCGLIGLIVT 70 80 90 100 110 120 GLIVVITEYY TGTNKRPVNS XAQASVTGHG TNVIQGLAVS LESTALPAIV IVGGIIXTYQ 130 140 LAGLFGTAIA VTAMLGIAGM I

References

[1]

"High prevalence of the H+ proton-pumping inorganic pyrophosphatase gene in alpha proteobacteria and evidence of lateral transfer in its phylogeny." Jumas-Bilak E., Michaux-Charachon S., Teyssier C. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF417515 Genomic DNA. Translation: AAL69331.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.6.1.1. 290736.
Family and domain databases
HAMAP	MF_01130. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HPPA_ANAMA

Accession

Primary (citable) accession number: Q8VRZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	March 1, 2002
Last modified:	October 13, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)