Q8VRH0 (PTG3C_STAEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PTS system glucose-specific EIICBA component

Alternative name(s):
EIICBA-Glc
Short name=EII-Glc

Including the following 3 domains:

1. Glucose permease IIC component
   Alternative name(s):
   PTS system glucose-specific EIIC component
2. Glucose-specific phosphotransferase enzyme IIB component
   EC=2.7.1.69
   Alternative name(s):
   PTS system glucose-specific EIIB component
3. Glucose-specific phosphotransferase enzyme IIA component
   EC=2.7.1.-
   Alternative name(s):
   PTS system glucose-specific EIIA component

Gene names

Name:	ptsG
Synonyms:	glcA

Organism

Staphylococcus epidermidis

Taxonomic identifier

1282 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	675 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate. Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Contains 1 PTS EIIA type-1 domain. Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glucose transmembrane transporter activity Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 675	675	PTS system glucose-specific EIICBA component		PRO_0000351402
Regions
	Transmembrane	16 – 36	21	Helical; Potential
	Transmembrane	59 – 79	21	Helical; Potential
	Transmembrane	81 – 101	21	Helical; Potential
	Transmembrane	126 – 146	21	Helical; Potential
	Transmembrane	170 – 190	21	Helical; Potential
	Transmembrane	211 – 231	21	Helical; Potential
	Transmembrane	273 – 293	21	Helical; Potential
	Transmembrane	303 – 323	21	Helical; Potential
	Transmembrane	328 – 348	21	Helical; Potential
	Transmembrane	355 – 375	21	Helical; Potential
	Transmembrane	378 – 398	21	Helical; Potential
	Domain	3 – 414	412	PTS EIIC type-1
	Domain	425 – 506	82	PTS EIIB type-1
	Domain	547 – 651	105	PTS EIIA type-1
Sites
	Active site	447	1	Phosphocysteine intermediate; for EIIB activity By similarity
	Active site	599	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8VRH0 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: AD6EC55F6C15C4C2	FASTA	675	73,359
	10 20 30 40 50 60 MFKKLFGQMQ RIGKALMLPV AILPAAGLLL AIGTAFQGEA LQHYLPFIKN DIVQQIANML 70 80 90 100 110 120 TGAGGIIFDN LPIIFALGVA IGLAGGDGVA AIAAFVGFII LNKTMGAFLH VTPDKLSDPT 130 140 150 160 170 180 NGYANVLGIP TLQTGVFGGI IIGALAAWCY NKFYNITLPS YLGFFAGKRF VPIMMATTSF 190 200 210 220 230 240 ILAFPMAIIW PTIQNGLNAF SEGLLDSNTG LAVFLFGFIK RLLIPFGLHH IFHAPFWFEF 250 260 270 280 290 300 GSWKNAAGEI IRGDQRIFIE QIREGAHLTS GKFMQGEFPV MMFGLPAAAL AIYQTAKPEN 310 320 330 340 350 360 KKVVAGLMIS AALTSFLTGI TEPLEFSFLF VAPFLFVIHA VLDGLSFLTL YLLNVHLGYT 370 380 390 400 410 420 FSGGFIDYVL LGILPNKTAW WLVIPVGIIY AVIYYFVFRF LIVKFNYKTP GREDKKSSVT 430 440 450 460 470 480 TTSASQLPFD VLKAMGGKEN IKHLDACITR LRVEVNEKSK VDVAGLKSLG ASGVLEVGNN 490 500 510 520 530 540 MQAIFGPKSD QIKHDMAKII SGEITKPSET TIDEEVSDDP VHVEDIVETE IYAPGHGEII 550 560 570 580 590 600 PLSEVPDKVF SEKMMGDGIG FVPDSGEIVA PFDGTVKTIF PTKHAIGLES DSGVEVLIHI 610 620 630 640 650 660 GIDTVKLNGE GFESLVNTDE PVTQGQPLMK IDLEYLKEHA PSIITPVIIT NQEDKTLTIE 670 DVKQVDPGKA IMTIK

References

[1]	"EII-glucose permease GlcA influences expression of polysaccharide intercellular adhesin." Kiel K., Knobloch J.K.M., Mack D. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Clinical isolate 1457.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF443793 Genomic DNA. Translation: AAL38016.1.
3D structure databases
HSSP	HSSP built from PDB template 1O2F based on UniProtKB P69786.
ProteinModelPortal	Q8VRH0.
SMR	Q8VRH0. Positions 431-500.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR011055. Dup_hybrid_motif. IPR018113. PTrfase_EIIB/Cys_phosph_CS. IPR001127. PTS_EIIA_1_perm. IPR001996. PTS_EIIB_1. IPR003352. PTS_EIIC. IPR013013. PTS_EIIC_1. IPR011535. PTS_Glc-like_IIB_component. IPR011299. PTS_IIBC_glc. [Graphical view]
Gene3D	G3DSA:3.30.1360.60. PTS_EIIB. 1 hit.
Pfam	PF00358. PTS_EIIA_1. 1 hit. PF00367. PTS_EIIB. 1 hit. PF02378. PTS_EIIC. 1 hit. [Graphical view]
SUPFAM	SSF51261. Dup_hybrid_motif. 1 hit. SSF55604. PTS_EIIB. 1 hit.
TIGRFAMs	TIGR00826. EIIB_glc. 1 hit. TIGR00830. PTBA. 1 hit. TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITE	PS51093. PTS_EIIA_TYPE_1. 1 hit. PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit. PS51098. PTS_EIIB_TYPE_1. 1 hit. PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit. PS51103. PTS_EIIC_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTG3C_STAEP

Accession

Primary (citable) accession number: Q8VRH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	March 1, 2002
Last modified:	September 21, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program