Q8VQD7 (Q8VQD7_THEAQ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Anti-cleavage anti-GreA transcription factor Gfh1 EMBL AAL57610.1

Gene names

Name:

gfh1 EMBL AAL57610.1

Organism

Thermus aquaticus EMBL AAL57610.1

Taxonomic identifier

271 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as greA or greB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides By similarity. SAAS SAAS018151

Ontologies

Keywords
Biological process	Transcription Transcription regulation SAAS SAAS001437
Ligand	DNA-binding SAAS SAAS001437
Technical term	3D-structure PDB 2ETN
Gene Ontology (GO)
Biological process	regulation of transcription elongation, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q8VQD7 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: F705CA040959ED46	FASTA	157	17,228
	10 20 30 40 50 60 MAREVKLTKA GYERLMKQLE QERERLQEAT KILQELMESS DDYDDSGLEA AKQEKARIEA 70 80 90 100 110 120 RIDSLEDVLS RAVILEEGTG EVIGLGSVVE LEDPATGERL SVQVVSPAEA SVLENPMKIS 130 140 150 DASPMGKALL GHRVGDVLSL DTPKGKKEFR VVAIHGR

References

[1]	"Transcript cleavage by Thermus thermophilus RNA polymerase: Effects of GreA and anti-GreA factors." Erie D.A., Hogan B.P., Hartsch T. J. Biol. Chem. 0:0-0(2002) Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Crystal structure of Thermus aquaticus Gfh1, a Gre-factor paralog that inhibits rather than stimulates transcript cleavage." Lamour V., Hogan B.P., Erie D.A., Darst S.A. J. Mol. Biol. 356:179-188(2006) [PubMed: 16337964] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.30 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF456233 Genomic DNA. Translation: AAL57610.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ETN	X-ray	3.30	A/B/C	1-157	[»]

ProteinModelPortal

Q8VQD7.

SMR

Q8VQD7. Positions 2-156.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

InterPro

IPR018151. TF_GreA/GreB_CS.
IPR023459. Tscrpt_elong_fac_GreA/B.
IPR001437. Tscrpt_elong_fac_GreA/B_C.
IPR022691. Tscrpt_elong_fac_GreA/B_N.
[Graphical view]

Gene3D

G3DSA:1.10.287.180. GreA_GreB_reg. 1 hit.
G3DSA:3.10.50.30. GreA_GreB_reg. 1 hit.

Pfam

PF01272. GreA_GreB. 1 hit.
PF03449. GreA_GreB_N. 1 hit.
[Graphical view]

PIRSF

PIRSF006092. GreA_GreB. 1 hit.

SUPFAM

SSF46557. GreA_GreB. 1 hit.

PROSITE

PS00830. GREAB_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q8VQD7_THEAQ

Accession

Primary (citable) accession number: Q8VQD7

Entry history

Integrated into UniProtKB/TrEMBL:	March 1, 2002
Last sequence update:	March 1, 2002
Last modified:	July 27, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)