Reviewed, UniProtKB/Swiss-Prot Q8VPZ0 (HPPA_RHIRD)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase

Gene names

Name:	hppA
Synonyms:	vppA

Organism

Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter)

Taxonomic identifier

358 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	189 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity.
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01130
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 2 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›189	›189	Pyrophosphate-energized proton pump HAMAP MF_01130		PRO_0000217008
Regions
	Transmembrane	30 – 52	23	Potential
	Transmembrane	74 – 96	23	Potential
	Transmembrane	141 – 163	23	Potential
	Transmembrane	168 – 187	20	Potential
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	189	1

Sequences

	Sequence		Length	Mass (Da)
	Q8VPZ0-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: E8CEB6C2DD06A6CD	FASTA	189	19,439
	10 20 30 40 50 60 AGGIAEMAEL GPEVRKTTDK LDSVGNTTAA IAKGFAIGSA ALTALALFTA FAEEIAKNPK 70 80 90 100 110 120 LTGLLVDGHL VVNLTEPGVI IGIFLGATLP FLVCAFTMEA VGKAAFEMIE EVRRQFREIP 130 140 150 160 170 180 GIMEGTGRPD YAKCVDISTK AAIREMMLPG VFAVGAPLIV GFLLGAKALA GFLAGVTASG VLLAIFSPS

References

[1]

"Presence of proton-translocating pyrophosphatase genes in root nodule-making bacteria (Rhizobia) and pathogenic tumour-making bacteria (Agrobacterium)." Perez-Castineira J.R., Losada M., Serrano A. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: D44.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ251785 Genomic DNA. Translation: CAC80979.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.6.1.1. 2199.
Family and domain databases
HAMAP	MF_01130. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HPPA_RHIRD

Accession

Primary (citable) accession number: Q8VPZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	March 1, 2002
Last modified:	October 13, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)