Q8VPF3 (CATI_PSESB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoadipate CoA-transferase subunit A
EC=2.8.3.6

Alternative name(s):
3-oxoadipate:succinyl-CoA transferase subunit A
Beta-ketoadipate:succinyl-CoA transferase subunit A

Gene names

Name:

catI

Organism

Pseudomonas sp. (strain B13)

Taxonomic identifier

65741 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the CoA transfer from succinate to 3-oxoadipate (beta-ketoadipate).
Catalytic activity	Succinyl-CoA + 3-oxoadipate = succinate + 3-oxoadipyl-CoA. Ref.2
Pathway	Aromatic compound metabolism; beta-ketoadipate pathway; acetyl-CoA and succinyl-CoA from 3-oxoadipate: step 1/2.
Subunit structure	Heterotetramer composed of 2 A and 2 B subunits. Ref.2
Sequence similarities	Belongs to the 3-oxoacid CoA-transferase subunit A family.
Biophysicochemical properties	Kinetic parameters: KM=0.4 mM for 3-oxoadipate Ref.2 KM=0.2 mM for succinyl-CoA pH dependence: Optimum pH is 8.4.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	beta-ketoadipate pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	3-oxoadipate CoA-transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 282	281	3-oxoadipate CoA-transferase subunit A		PRO_0000337673

Sequences

	Sequence		Length	Mass (Da)
	Q8VPF3 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: EFC6A128C8F8CB34	FASTA	282	30,973
	10 20 30 40 50 60 MAELLTLREA VERFVNDGDT VALEGFTHLI PTAASHEIIR QGKKDLHLVR MTPDLVYDLL 70 80 90 100 110 120 IGAGCARKLT FSWGGNPGVG SLHRLRDAVE KGWPNALEID EHSHADLANS YVAGASGLPF 130 140 150 160 170 180 AVLRAYAGSD LPKVNPNIKF INCPFTGEQL AAVPSVRPDV TVIHAQKADR KGNVLLWGIL 190 200 210 220 230 240 GVQKEAALAA KRCIVTVEEI VDDLNAPMNS CVLPTWALSA VCHVPGGSHP SYAHGYYERD 250 260 270 280 NRFYQAWDPI ARDRETFTAW IDEYIRGTKD FSEFQAKIAE GK

References

[1]

"Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: cloning, characterization, and analysis of sequences encoding 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase." Goebel M., Kassel-Cati K., Schmidt E., Reineke W. J. Bacteriol. 184:216-223(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

[2]

"Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: purification and characterization of 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase." Kaschabek S.R., Kuhn B., Mueller D., Schmidt E., Reineke W. J. Bacteriol. 184:207-215(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-31, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY044272 Genomic DNA. Translation: AAL02405.1.
3D structure databases
HSSP	HSSP built from PDB template 1POI based on UniProtKB Q59111.
ProteinModelPortal	Q8VPF3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00157; UER00262.
Family and domain databases
InterPro	IPR004165. CoA_trans_fam_I. [Graphical view]
PANTHER	PTHR13707. PTHR13707. 1 hit.
Pfam	PF01144. CoA_trans. 1 hit. [Graphical view]
SMART	SM00882. CoA_trans. 1 hit. [Graphical view]
PROSITE	PS01273. COA_TRANSF_1. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CATI_PSESB

Accession

Primary (citable) accession number: Q8VPF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 1, 2002
Last modified:	April 3, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program