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Q8VPF3 (CATI_PSESB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-oxoadipate CoA-transferase subunit A

EC=2.8.3.6
Alternative name(s):
3-oxoadipate:succinyl-CoA transferase subunit A
Beta-ketoadipate:succinyl-CoA transferase subunit A
Gene names
Name:catI
OrganismPseudomonas sp. (strain B13)
Taxonomic identifier65741 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the CoA transfer from succinate to 3-oxoadipate (beta-ketoadipate).

Catalytic activity

Succinyl-CoA + 3-oxoadipate = succinate + 3-oxoadipyl-CoA. Ref.2

Pathway

Aromatic compound metabolism; beta-ketoadipate pathway; acetyl-CoA and succinyl-CoA from 3-oxoadipate: step 1/2.

Subunit structure

Heterotetramer composed of 2 A and 2 B subunits. Ref.2

Sequence similarities

Belongs to the 3-oxoacid CoA-transferase subunit A family.

Biophysicochemical properties

Kinetic parameters:

KM=0.4 mM for 3-oxoadipate Ref.2

KM=0.2 mM for succinyl-CoA

pH dependence:

Optimum pH is 8.4.

Ontologies

Keywords
   Biological processAromatic hydrocarbons catabolism
   Molecular functionTransferase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processbeta-ketoadipate pathway

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_function3-oxoadipate CoA-transferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2
Chain2 – 2822813-oxoadipate CoA-transferase subunit A
PRO_0000337673

Sequences

Sequence LengthMass (Da)Tools
Q8VPF3 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: EFC6A128C8F8CB34

FASTA28230,973
        10         20         30         40         50         60 
MAELLTLREA VERFVNDGDT VALEGFTHLI PTAASHEIIR QGKKDLHLVR MTPDLVYDLL 

        70         80         90        100        110        120 
IGAGCARKLT FSWGGNPGVG SLHRLRDAVE KGWPNALEID EHSHADLANS YVAGASGLPF 

       130        140        150        160        170        180 
AVLRAYAGSD LPKVNPNIKF INCPFTGEQL AAVPSVRPDV TVIHAQKADR KGNVLLWGIL 

       190        200        210        220        230        240 
GVQKEAALAA KRCIVTVEEI VDDLNAPMNS CVLPTWALSA VCHVPGGSHP SYAHGYYERD 

       250        260        270        280 
NRFYQAWDPI ARDRETFTAW IDEYIRGTKD FSEFQAKIAE GK 

« Hide

References

[1]"Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: cloning, characterization, and analysis of sequences encoding 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase."
Goebel M., Kassel-Cati K., Schmidt E., Reineke W.
J. Bacteriol. 184:216-223(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: purification and characterization of 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase."
Kaschabek S.R., Kuhn B., Mueller D., Schmidt E., Reineke W.
J. Bacteriol. 184:207-215(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-31, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY044272 Genomic DNA. Translation: AAL02405.1.

3D structure databases

ProteinModelPortalQ8VPF3.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKQ8VPF3.
UniPathwayUPA00157; UER00262.

Family and domain databases

InterProIPR004165. CoA_trans_fam_I.
[Graphical view]
PANTHERPTHR13707. PTHR13707. 1 hit.
PfamPF01144. CoA_trans. 1 hit.
[Graphical view]
SMARTSM00882. CoA_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCATI_PSESB
AccessionPrimary (citable) accession number: Q8VPF3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 1, 2002
Last modified: April 16, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways