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Protein

3-oxoadipate CoA-transferase subunit B

Gene

catJ

Organism
Pseudomonas knackmussii (strain DSM 6978 / LMG 23759 / B13)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Succinyl-CoA + 3-oxoadipate = succinate + 3-oxoadipyl-CoA.1 Publication

Kineticsi

  1. KM=0.4 mM for 3-oxoadipate1 Publication
  2. KM=0.2 mM for succinyl-CoA1 Publication

    pH dependencei

    Optimum pH is 8.4.1 Publication

    Pathway: beta-ketoadipate pathway

    This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA and succinyl-CoA from 3-oxoadipate.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. 3-oxoadipate CoA-transferase subunit B (catJ), 3-oxoadipate CoA-transferase subunit A (catI)
    2. Beta-ketoadipyl-CoA thiolase (pcaF)
    This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA and succinyl-CoA from 3-oxoadipate, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei51 – 511By similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism

    Enzyme and pathway databases

    BRENDAi2.8.3.6. 5180.
    SABIO-RKQ8VPF2.
    UniPathwayiUPA00157; UER00262.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-oxoadipate CoA-transferase subunit B (EC:2.8.3.6)
    Alternative name(s):
    3-oxoadipate:succinyl-CoA transferase subunit B
    Beta-ketoadipate:succinyl-CoA transferase subunit B
    Gene namesi
    Name:catJ
    OrganismiPseudomonas knackmussii (strain DSM 6978 / LMG 23759 / B13)
    Taxonomic identifieri1301098 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 2602593-oxoadipate CoA-transferase subunit BPRO_0000337674Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer composed of 2 A and 2 B subunits.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VPF2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR004165. CoA_trans_fam_I.
    [Graphical view]
    PANTHERiPTHR13707. PTHR13707. 1 hit.
    PfamiPF01144. CoA_trans. 1 hit.
    [Graphical view]
    SMARTiSM00882. CoA_trans. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8VPF2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSAYSTNEMM TVAAARRLKN GAVCFVGIGL PSKAANLARL TSSPDVVLIY
    60 70 80 90 100
    ESGPIGAKPT VLPLSIGDGE LAETADTVVP TGEIFRYWLQ GGRIDVGFLG
    110 120 130 140 150
    AAQVDRFGNI NTTVIGDYNK PKVRLPGAGG APEIAGSAKE VLIILKQSHR
    160 170 180 190 200
    TFVDKLAFIT SVGHGEGGDH RKQLGLPGKG PVAIITDLCI MEPEAGSNEF
    210 220 230 240 250
    IVTSLHPGVT REQVIENTGW AIRFAEQVKE TAAPTEVELE ALRALEARTA
    260
    AAHGQQGGEE
    Length:260
    Mass (Da):27,383
    Last modified:March 1, 2002 - v1
    Checksum:iCC8FABC48FC1D051
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY044272 Genomic DNA. Translation: AAL02406.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY044272 Genomic DNA. Translation: AAL02406.1.

    3D structure databases

    ProteinModelPortaliQ8VPF2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    UniPathwayiUPA00157; UER00262.
    BRENDAi2.8.3.6. 5180.
    SABIO-RKQ8VPF2.

    Family and domain databases

    InterProiIPR004165. CoA_trans_fam_I.
    [Graphical view]
    PANTHERiPTHR13707. PTHR13707. 1 hit.
    PfamiPF01144. CoA_trans. 1 hit.
    [Graphical view]
    SMARTiSM00882. CoA_trans. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    1. "Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: cloning, characterization, and analysis of sequences encoding 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase."
      Goebel M., Kassel-Cati K., Schmidt E., Reineke W.
      J. Bacteriol. 184:216-223(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: purification and characterization of 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase."
      Kaschabek S.R., Kuhn B., Mueller D., Schmidt E., Reineke W.
      J. Bacteriol. 184:207-215(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-26, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

    Entry informationi

    Entry nameiCATJ_PSEKB
    AccessioniPrimary (citable) accession number: Q8VPF2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: March 1, 2002
    Last modified: June 24, 2015
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.