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Reviewed, UniProtKB/Swiss-Prot Q8VPB5 (ACSF_RHOGE)

Last modified January 20, 2009. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
      Short name=Aerobic Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81
Gene names
Name: acsF
OrganismRhodocyclus gelatinosus (Rhodopseudomonas gelatinosa)
Taxonomic identifier28068 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Rubrivivax

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Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis in aerobic conditions. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME). Ref.1

Catalytic activity

Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O. HAMAP MF_01840

131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O. HAMAP MF_01840

131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O. HAMAP MF_01840

Cofactor

Iron By similarity.

Pathway

Porphyrin biosynthesis; chlorophyll biosynthesis. HAMAP MF_01840

Disruption phenotype

No phenotype under photosynthesis or low-aeration respiratory growth conditions. In contrast, under highly aerated respiratory growth conditions, the absence of acsF leads to an accumulation of MgPMME. Ref.1

Sequence similarities

Belongs to the acsF family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 358358Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase HAMAP MF_01840
PRO_0000217533

Experimental info

Sequence conflict701A → P in BAA94050. Ref.2
Sequence conflict1281N → D in BAA94050. Ref.2
Sequence conflict1481N → D in BAA94050. Ref.2
Sequence conflict229 – 2313SGA → TGP in BAA94050. Ref.2
Sequence conflict2561Q → L in BAA94050. Ref.2
Sequence conflict2931Q → P in BAA94050. Ref.2
Sequence conflict3041R → H in BAA94050. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Q8VPB5-1 [UniParc].

Last modified July 5, 2004. Version 2.
Checksum: 299E24EFFD4BFD9C

FASTA35841,513
        10         20         30         40         50         60 
MLATPTIESP EEAARRAKES TLLSPRFYTT DYAAMNAIDV SSIRAEWDAM LAEYEGDNNH 

        70         80         90        100        110        120 
DHFQRTPEFA QEVAERFSQV SPELRQEFLD FLVSSVTSEF SGCVLYNEIQ KNVENPDVKA 

       130        140        150        160        170        180 
LMRYMARNES RHAGFINQAL RDFGLGINLG GLKRTKAYTY FKPKYIFYAT YLSEKIGYAR 

       190        200        210        220        230        240 
YITIYRQLER HPDKRFHPIF RWFERWCNDE FRHGESFALI LRAHPHLISG ANLLWVRFFL 

       250        260        270        280        290        300 
LAVYATMYVR DHMRPQLHEA MGLESTDYDY RVFQITNEIS KQVFPISLDI DHQAFRAGME 

       310        320        330        340        350 
RLVRVKTKVD AAKARGGLVG RLQQAAWAAA GAATFARMYL IPVRRHALPA QVRMAPAW

« Hide

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References

[1]"Rubrivivax gelatinosus acsF (previously orf358) codes for a conserved, putative binuclear-iron-cluster-containing protein involved in aerobic oxidative cyclization of Mg-protoporphyrin IX monomethylester."
Pinta V., Picaud M., Reiss-Husson F., Astier C.
J. Bacteriol. 184:746-753(2002) [PubMed: 11790744] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE.
Strain: S1.
[2]"Determination of nucleotide sequences of Rubrivivax gelatinosus Photosynthetic genes."
Nagashima K.V., Igarashi N., Harada J., Nagashima S., Matsuura K., Shimada K.
Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: IL144.

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Cross-references

Sequence databases

AY234384 Genomic DNA. Translation: AAL25840.2.
AB034704 Genomic DNA. Translation: BAA94050.1.
PIRT50897.

3D structure databases

ModBaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MON-13266.
BRENDA1.14.13.81. 271297.

Family and domain databases

HAMAPMF_01840.
[Tree]
InterProIPR008434. AcsF.
IPR003251. Rubrerythrin.
[Graphical view]
PfamPF02915. Rubrerythrin. 1 hit.
[Graphical view]
TIGRFAMsTIGR02029. AcsF. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameACSF_RHOGE
AccessionPrimary (citable) accession number: Q8VPB5
Secondary accession number(s): Q9JPB1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: July 5, 2004
Last modified: January 20, 2009
This is version 37 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents